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A chicken embryo gene expression database 		 
GEISHA - A Chicken Embryo Gene Expression Database
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Gene Expression Report

Probe 1

GEISHA IdTbx5.Peters.2002
 NCBIEnsembl
Gene Name TBX5_CHICK
Gene Description T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
Ensembl Genome Map
contig view
CitationPeters MA, Cepko CL (2002). The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments. Dev Biol. 2002 Nov 1;251(1):59-73.
Copyright"Reprinted from Developmental Biology, 251(1), Peters MA, Cepko CL, The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments, 59-73, Copyright (2002), with permission from Elsevier". Copyright © ( 2002) Elsevier B.V. All rights reserved.
CommentsAccording to authors , "Probes for Pax2, Bmp4, Tbx5, SOHo, ephrin-B1, ephrin-B2, EphB1-B3, ALDH3/ALDH6, and ALDH1 were prepared as described (Deitcher et al., 1994; Godbout et al., 1996; Holash et al., 1997; Logan et al., 1998; Golden et al., 1999; Grun et al., 2000)." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204173).
Complete Sequenceshow
CGGGGGGCTGCCGCCATGCGGCCCCACGGTGCGGGGGTTCCTCCGGGCAGGAAGGCGGGGGCCGACCCTGCTGTCCGCA
TCGCAGCGAGGAGCCGCAGCCACGCACCCCCCCGCACGCGCAGTGGGGGATTCGGCGAAGGAAGCTCGTAACATGGCGG
ACACCGAGGAAGGCTTCGGGCTCCCGAGCACGCCGGTTGACTCGGAGGCCAAGGAGCTGCAGGCTGAGGCCAAGCAGGA
TCCCCAGCTGGGGACCACCAGCAAGGCCCCCACCTCTCCACAGGCGGCCTTCACCCAGCAGGGCATGGAGGGGATCAAA
GTGTTTTTGCACGAGCGGGAGCTGTGGCTGAAATTTCACGAGGTGGGGACGGAGATGATCATAACAAAGGCTGGAAGGC
GTATGTTTCCCAGTTACAAAGTGAAGGTCACTGGACTCAATCCAAAAACGAAGTACATACTGTTGATGGATATTGTACC
AGCGGATGACCACAGATACAAATTTGCAGATAATAAATGGTCCGTGACCGGGAAGGCAGAACCGGCCATGCCCGGCCGC
CTCTACGTGCACCCCGACTCCCCCGCTACTGGAGCCCACTGGATGAGGCAGTTGGTTTCCTTCCAGAAGCTCAAGCTCA
CCAACAACCACCTTGACCCCTTCGGACATATCATCCTGAACTCCATGCACAAATACCAGCCCCGGCTCCACATCGTGAA
GGCGGATGAGAACAACGGCTTTGGCTCCAAGAACACTGCCTTCTGCACCCATGTCTTCCCCGAGACTGCCTTCATCGCT
GTTACCTCCTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTCGCAAAAGGTTTCCGCGGCAGCG
ATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACAGAA
AGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTACCAG
TGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCGCCCACCAACCCCTACCCGATCTCCCAGGAGCACAGCC
AGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGAAAC
TTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACTGAA
TCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCAGCT
GTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTACCA
GCATTTCTCTGCCCACTTCACCTCGGGGCCTCTGATGCCCCGGCTCAGCAGCGTGGCCAACCACACGTCCCCCCAGATA
GGAGACACCCATAGCATGTTCCAGCACCAGACCTCAGTTTCTCACCAACCCATTGTGCGGCAGTGTGGACCTCAGACCG
GCATCCAGTCTCCCCCCAGCAGCTTGCAGCCTGCAGAGTTCCTCTATTCCCACGGCGTGCCTCGAACCCTCTCGCCCCA
CCAGTACCACTCGGTGCACGGTGTGGGCATGGTGCCAGAGTGGAGCGAGAACAGCTAACGAGGCAGTCGATGGAAATGG
GAAAAAAAATAAAACGAAATGAAAGAAAAAAGTGAAGGGGGAAATAAGAAAAAAGGAAAGGGAAAACAAAACAAAACAA
AACAAAAAACCAGCACCCCATCAATAACAAAAACGAGAGCGTTTTGCAAGTCTCCTTTAACCGAATGTTCACCGTCAGC
ACTCGAGAGGGATGGACGCTGACCGTGCCACTGGTTGGAAGCACGGATGGAGCACTCATTGGCCATGGCACGATGTGCC
CCCCCCGGTGCTTTGGTTCAGAAGGCAAACCCAGCAGAGCGGAGCGCTGCCCCCACGTCCCCCCAGTCTGTGTCGCTGC
CACAGCCATCCCCTGCCTGGCCCTCCCCTGCCCCTCTCCTTCCCAAAGGGACTCGCGACTCCTGCTGCCTTCCTCAACA
CAGCCAGAGTGCTGGGATGAAAACAACTAGGATGTTAC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
13-18
stage 18

Eye

 
25-30
stage 22

Eye

 
31-44
stage 44
stage 44

Eye

 

Probe 2

GEISHA IdChEST59k10
 NCBIEnsembl
Gene NameTBX5TBX5_CHICK
AliasesTBX5, T-box 5, Official 
Gene DescriptionT-box 5T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003700 transcription factor activity ISS
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005634 nucleus ISS
GO:0005737 cytoplasm ISS
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008134 transcription factor binding IEA
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter IEA
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030336 negative regulation of cell migration ISS
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0055007 cardiac muscle cell differentiation IEA
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
show
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
NCBI Nucleotide HitNM_204173 show details
scoreevaluealignment lengthdescription
6790.0695Gallus gallus T-box 5 (TBX5), mRNA
Ensembl Genome Map
contig view
5' Sequenceshow
GCCACAGGACGGCTGTTTACCCTGCGCTGACCTATTCAACTTTACCCTCAAAATAATACATTAAAAAGAGAGCGAGAAG
GGAGAGGAGGAAGAGGCAGATAAAACCACGTCCCCGCTCCTCGCTGACGGTTTGATTATTTGAGCAGAGCGGAGGCTGC
GGGGTTGCGTACCCCAGCCGGGCAGCGCGTGGGGGATTCGGCGAAGGAAGCTCGTAACATGGCGGACACCGAGGAAGGC
TTCGGGCTCCCGAGCACGCCGGTTGACTCGGAGGCCAAGGAGCTGCAGGCTGAGGCCAAGCAGGATCCCCAGCTGGGGA
CCACCAGCAAGGCCCCCACCTCTCCACAGGCGGCCTTCACCCAGCAGGGCATGGAGGGGATCAAAGTGTTTTTGCACGA
GCGGGAGCTGTGGCTGAAATTTCACGAGGTGGGGACGGAGATGATCATAACAAAGGCTGGAAGGCGTATGTTTCCCAGT
TACAAAGTGAAGGTCACTGGACTCAATCCAAAAACGAAGTACATACTGTTGATGGATATTGTACCAGCGGATGACCACA
GATACAAATTTGCAGATAATAAATGGTCCGTGACCGGGAAGGCAGAACCGGCCATGCCCGGCCGCCTCTACGTGCACCC
CGACTCCCCCGCTACTGGAGCCCACTGGATGAGGCAGTTGGTTTCCTTCCAGAAGCTCAAGCTCACCAACAACCACCTT
GACCCCTTCGGACATATCATCCTGAACTCCATGCACAAATACCAGCCCCGGCTCCACATCGTGAAGGCGGGATGAGAAC
ACGGCTTTGGCTCCAAGAACACTGCCTTCTGCACCCATGTCTTCCCCGAGACTGCCTTCATCGCTGTTACCTCCTACCA
AAACCACAAGAGATGTAAGGGGGAAGCTACTTAACGTGCATGAAATGGTCGCGTTTTCTCTATCCATCGGTTGGGCCGG
GAGCTGCAAAAACGGAAGTAGGCTTGAAAAAAAAAAATGT
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 4
stage 6

Early Mesoderm

Primitive Streak

Splanchnic Mesoderm

 
7-12
stage 10
stage 10
stage 11
stage 12
stage 12

Atria

Heart Tube

Sinus Venosus

Telencephalon

Ventricles

 
13-18
stage 13
stage 13
stage 14
stage 17
stage 17
stage 17
stage 18
stage 18
stage 18

Allantois

Atria

Eye

Heart Tube

Lateral Plate Mesoderm

Retina

Sinus Venosus

Somatopleure

Telencephalon

Ventricles

Wing

Wing Mesenchyme

 
19-22
stage 19
stage 19
stage 20
stage 22
stage 22

Allantois

Atria

Eye

Retina

Somatopleure

Splanchnic Mesoderm

Ventricles

Wing Mesenchyme

hand transected

Probe 3

GEISHA IdTbx5.Canto-Soler.2006
 NCBIEnsembl
Gene NameTBX5TBX5_CHICK
AliasesTBX5, T-box 5, Official 
Gene DescriptionT-box 5T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003700 transcription factor activity ISS
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005634 nucleus ISS
GO:0005737 cytoplasm ISS
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008134 transcription factor binding IEA
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter IEA
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030336 negative regulation of cell migration ISS
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0055007 cardiac muscle cell differentiation IEA
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
show
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
NCBI Nucleotide HitNM_204173 show details
scoreevaluealignment lengthdescription
21710.02171Gallus gallus T-box 5 (TBX5), mRNA
Ensembl Genome Map
contig view
CitationCanto-Soler MV, Adler R. Optic cup and lens development requires Pax6 expression in the early optic vesicle during a narrow time window. Dev Biol. 2006 Jun 1;294(1):119-32. Epub 2006 Mar 27.
CopyrightReprinted from Developmental Biology, 294, Issue 1, M. Valeria Canto-Soler and Ruben Adler, Optic cup and lens development requires Pax6 expression in the early optic vesicle during a narrow time window, 119-132, 2006, with permission from Elsevier. Copyright © 2006 Elsevier B.V. All rights
CommentsThe authors state: Probes were generated from cDNAs generously provided by the following investigators: cTbx5 (Katherine Yutzey, Children's Hospital Medical Center, Cincinnati, OH). As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204173).
Complete Sequenceshow
CGGGGGGCTGCCGCCATGCGGCCCCACGGTGCGGGGGTTCCTCCGGGCAGGAAGGCGGGGGCCGACCCTGCTGTCCGCA
TCGCAGCGAGGAGCCGCAGCCACGCACCCCCCCGCACGCGCAGTGGGGGATTCGGCGAAGGAAGCTCGTAACATGGCGG
ACACCGAGGAAGGCTTCGGGCTCCCGAGCACGCCGGTTGACTCGGAGGCCAAGGAGCTGCAGGCTGAGGCCAAGCAGGA
TCCCCAGCTGGGGACCACCAGCAAGGCCCCCACCTCTCCACAGGCGGCCTTCACCCAGCAGGGCATGGAGGGGATCAAA
GTGTTTTTGCACGAGCGGGAGCTGTGGCTGAAATTTCACGAGGTGGGGACGGAGATGATCATAACAAAGGCTGGAAGGC
GTATGTTTCCCAGTTACAAAGTGAAGGTCACTGGACTCAATCCAAAAACGAAGTACATACTGTTGATGGATATTGTACC
AGCGGATGACCACAGATACAAATTTGCAGATAATAAATGGTCCGTGACCGGGAAGGCAGAACCGGCCATGCCCGGCCGC
CTCTACGTGCACCCCGACTCCCCCGCTACTGGAGCCCACTGGATGAGGCAGTTGGTTTCCTTCCAGAAGCTCAAGCTCA
CCAACAACCACCTTGACCCCTTCGGACATATCATCCTGAACTCCATGCACAAATACCAGCCCCGGCTCCACATCGTGAA
GGCGGATGAGAACAACGGCTTTGGCTCCAAGAACACTGCCTTCTGCACCCATGTCTTCCCCGAGACTGCCTTCATCGCT
GTTACCTCCTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTCGCAAAAGGTTTCCGCGGCAGCG
ATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACAGAA
AGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTACCAG
TGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCGCCCACCAACCCCTACCCGATCTCCCAGGAGCACAGCC
AGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGAAAC
TTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACTGAA
TCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCAGCT
GTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTACCA
GCATTTCTCTGCCCACTTCACCTCGGGGCCTCTGATGCCCCGGCTCAGCAGCGTGGCCAACCACACGTCCCCCCAGATA
GGAGACACCCATAGCATGTTCCAGCACCAGACCTCAGTTTCTCACCAACCCATTGTGCGGCAGTGTGGACCTCAGACCG
GCATCCAGTCTCCCCCCAGCAGCTTGCAGCCTGCAGAGTTCCTCTATTCCCACGGCGTGCCTCGAACCCTCTCGCCCCA
CCAGTACCACTCGGTGCACGGTGTGGGCATGGTGCCAGAGTGGAGCGAGAACAGCTAACGAGGCAGTCGATGGAAATGG
GAAAAAAAATAAAACGAAATGAAAGAAAAAAGTGAAGGGGGAAATAAGAAAAAAGGAAAGGGAAAACAAAACAAAACAA
AACAAAAAACCAGCACCCCATCAATAACAAAAACGAGAGCGTTTTGCAAGTCTCCTTTAACCGAATGTTCACCGTCAGC
ACTCGAGAGGGATGGACGCTGACCGTGCCACTGGTTGGAAGCACGGATGGAGCACTCATTGGCCATGGCACGATGTGCC
CCCCCCGGTGCTTTGGTTCAGAAGGCAAACCCAGCAGAGCGGAGCGCTGCCCCCACGTCCCCCCAGTCTGTGTCGCTGC
CACAGCCATCCCCTGCCTGGCCCTCCCCTGCCCCTCTCCTTCCCAAAGGGACTCGCGACTCCTGCTGCCTTCCTCAACA
CAGCCAGAGTGCTGGGATGAAAACAACTAGGATGTTAC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
7-12
stage 11

Retina

 
13-18
stage 18

Retina

 

Probe 4

GEISHA IdTbx5.Hurtado.2006
 NCBIEnsembl
Gene NameTBX5TBX5_CHICK
AliasesTBX5, T-box 5, Official 
Gene DescriptionT-box 5T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003700 transcription factor activity ISS
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005634 nucleus ISS
GO:0005737 cytoplasm ISS
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008134 transcription factor binding IEA
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter IEA
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030336 negative regulation of cell migration ISS
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0055007 cardiac muscle cell differentiation IEA
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
show
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
NCBI Nucleotide HitNM_204173 show details
scoreevaluealignment lengthdescription
21710.02171Gallus gallus T-box 5 (TBX5), mRNA
Ensembl Genome Map
contig view
CitationHurtado R, Mikawa T. Enhanced sensitivity and stability in two-color in situ hybridization by means of a novel chromagenic substrate combination. Dev Dyn. 2006 Oct;235(10):2811-6.
CopyrightCopyright © 2006 Wiley-Liss, Inc.
CommentsInsufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204173).
Complete Sequenceshow
CGGGGGGCTGCCGCCATGCGGCCCCACGGTGCGGGGGTTCCTCCGGGCAGGAAGGCGGGGGCCGACCCTGCTGTCCGCA
TCGCAGCGAGGAGCCGCAGCCACGCACCCCCCCGCACGCGCAGTGGGGGATTCGGCGAAGGAAGCTCGTAACATGGCGG
ACACCGAGGAAGGCTTCGGGCTCCCGAGCACGCCGGTTGACTCGGAGGCCAAGGAGCTGCAGGCTGAGGCCAAGCAGGA
TCCCCAGCTGGGGACCACCAGCAAGGCCCCCACCTCTCCACAGGCGGCCTTCACCCAGCAGGGCATGGAGGGGATCAAA
GTGTTTTTGCACGAGCGGGAGCTGTGGCTGAAATTTCACGAGGTGGGGACGGAGATGATCATAACAAAGGCTGGAAGGC
GTATGTTTCCCAGTTACAAAGTGAAGGTCACTGGACTCAATCCAAAAACGAAGTACATACTGTTGATGGATATTGTACC
AGCGGATGACCACAGATACAAATTTGCAGATAATAAATGGTCCGTGACCGGGAAGGCAGAACCGGCCATGCCCGGCCGC
CTCTACGTGCACCCCGACTCCCCCGCTACTGGAGCCCACTGGATGAGGCAGTTGGTTTCCTTCCAGAAGCTCAAGCTCA
CCAACAACCACCTTGACCCCTTCGGACATATCATCCTGAACTCCATGCACAAATACCAGCCCCGGCTCCACATCGTGAA
GGCGGATGAGAACAACGGCTTTGGCTCCAAGAACACTGCCTTCTGCACCCATGTCTTCCCCGAGACTGCCTTCATCGCT
GTTACCTCCTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTCGCAAAAGGTTTCCGCGGCAGCG
ATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACAGAA
AGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTACCAG
TGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCGCCCACCAACCCCTACCCGATCTCCCAGGAGCACAGCC
AGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGAAAC
TTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACTGAA
TCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCAGCT
GTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTACCA
GCATTTCTCTGCCCACTTCACCTCGGGGCCTCTGATGCCCCGGCTCAGCAGCGTGGCCAACCACACGTCCCCCCAGATA
GGAGACACCCATAGCATGTTCCAGCACCAGACCTCAGTTTCTCACCAACCCATTGTGCGGCAGTGTGGACCTCAGACCG
GCATCCAGTCTCCCCCCAGCAGCTTGCAGCCTGCAGAGTTCCTCTATTCCCACGGCGTGCCTCGAACCCTCTCGCCCCA
CCAGTACCACTCGGTGCACGGTGTGGGCATGGTGCCAGAGTGGAGCGAGAACAGCTAACGAGGCAGTCGATGGAAATGG
GAAAAAAAATAAAACGAAATGAAAGAAAAAAGTGAAGGGGGAAATAAGAAAAAAGGAAAGGGAAAACAAAACAAAACAA
AACAAAAAACCAGCACCCCATCAATAACAAAAACGAGAGCGTTTTGCAAGTCTCCTTTAACCGAATGTTCACCGTCAGC
ACTCGAGAGGGATGGACGCTGACCGTGCCACTGGTTGGAAGCACGGATGGAGCACTCATTGGCCATGGCACGATGTGCC
CCCCCCGGTGCTTTGGTTCAGAAGGCAAACCCAGCAGAGCGGAGCGCTGCCCCCACGTCCCCCCAGTCTGTGTCGCTGC
CACAGCCATCCCCTGCCTGGCCCTCCCCTGCCCCTCTCCTTCCCAAAGGGACTCGCGACTCCTGCTGCCTTCCTCAACA
CAGCCAGAGTGCTGGGATGAAAACAACTAGGATGTTAC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
13-18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18

Atria

Ear/Otic Placcode

Eye

Heart

Hindbrain

Limb Buds

Nephric Ducts

Ventricles

Wing

 

Probe 5

GEISHA IdTbx5.Liberatore.2000
 NCBIEnsembl
Gene NameTBX5TBX5_CHICK
AliasesTBX5, T-box 5, Official 
Gene DescriptionT-box 5T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003700 transcription factor activity ISS
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005634 nucleus ISS
GO:0005737 cytoplasm ISS
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008134 transcription factor binding IEA
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter IEA
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030336 negative regulation of cell migration ISS
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0055007 cardiac muscle cell differentiation IEA
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
show
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
NCBI Nucleotide HitAF033671 show details
scoreevaluealignment lengthdescription
6570.0695Gallus gallus T-Box protein 5 (TBX5) mRNA, complete cds
Ensembl Genome Map
contig view
CitationLiberatore CM, Searcy-Schrick RD, Yutzey KE. Ventricular expression of tbx5 inhibits normal heart chamber development. Dev Biol. 2000 Jul 1;223(1):169-80.
CopyrightReprinted with permission from Elsevier. Copyright © date Elsevier B.V. All rights reserved.
CommentsAccording to the authors, "Oligonucleotide primers for PCR were used to amplify nucleotides from +648 to +1361 of the reported chicken tbx5 sequence (Isaac et al., 1998) (GenBank Accession No. AF033671)." Sequence below was obtained from NCBI using this information.
Complete Sequenceshow
GAAGGCGGATGAGAACAACGGCTTTGGGTCCAAGAACACTGCCTTCTGCACCCATGTNTTCCCCGAGACTGCCTTCATT
GCTGTTACCTCNTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTTGCAAAAGGTTTCCGCGGCA
GCGATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACA
GAAAGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTAC
CAGTGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCACCCACCAACCCCTACCCGATCTCCCAGGAGCACA
GCCAGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGA
AACTTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACT
GAATCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCA
GCTGTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTA
CAG
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
7-12
stage 8
stage 9
stage 10
stage 12

Atria

Heart

Heart Tube

Mesoderm

A-V Junction

Cardiogenic Mesoderm

Posterior Atrial Region

13-14
stage 14
stage 14

Atria

Eye

Heart

Limb Buds

Pharyngeal Arches and Clefts

Wing

A-V Junction

Figure 1 compares the expression of Tbx5, Nkx2-5 and AMHC1 in the developing heart at stages HH10, HH12 and HH14.

Posterior Atrial Region

Probe 6

GEISHA IdTbx5.Lincoln.2004
 NCBIEnsembl
Gene NameTBX5TBX5_CHICK
AliasesTBX5, T-box 5, Official 
Gene DescriptionT-box 5T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003700 transcription factor activity ISS
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005634 nucleus ISS
GO:0005737 cytoplasm ISS
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008134 transcription factor binding IEA
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter IEA
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030336 negative regulation of cell migration ISS
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0055007 cardiac muscle cell differentiation IEA
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
show
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
NCBI Nucleotide HitNM_204173 show details
scoreevaluealignment lengthdescription
6900.0712Gallus gallus T-box 5 (TBX5), mRNA
Ensembl Genome Map
contig view
CitationLincoln J, Alfieri CM, Yutzey KE. Development of heart valve leaflets and supporting apparatus in chicken and mouse embryos. Dev Dyn. 2004 Jun;230(2):239-50.
CopyrightWiley-Liss, Inc. 2004
Complete Sequenceshow
GAAGGCGGATGAGAACAACGGCTTTGGGTCCAAGAACACTGCCTTCTGCACCCATGTNTTCCCCGAGACTGCCTTCATT
GCTGTTACCTCNTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTTGCAAAAGGTTTCCGCGGCA
GCGATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACA
GAAAGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTAC
CAGTGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCACCCACCAACCCCTACCCGATCTCCCAGGAGCACA
GCCAGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGA
AACTTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACT
GAATCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCA
GCTGTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTA
CAGCA
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
25-30
stage 29

Heart

Valves

atrial septa

31-36
stage 36

Heart

Valves

 

Probe 7

GEISHA IdTbx5.Maden.2007
 NCBIEnsembl
Gene Name TBX5_CHICK
Gene Description T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
Ensembl Genome Map
contig view
CitationMaden M, Blentic A, Reijntjes S, Seguin S, Gale E, Graham A. (2007). Retinoic acid is required for specification of the ventral eye field and for Rathke's pouch in the avian embryo. Int J Dev Biol. 2007;51(3):191-200.
Copyright"Reprinted from Int. J. Dev. Biol., 51, Maden M, Blentic A, Reijntjes S, Seguin S, Gale E, Graham A, Retinoic acid is required for specification of the ventral eye field and for Rathke's pouch in the avian embryo, 191-200, Copyright (2007), with permission from Copyright © 2007 UBC Press". All rights reserved.
CommentsInsufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_204173). Images are quail, but sequence and map shown are chick.
Complete Sequenceshow
CGGGGGGCTGCCGCCATGCGGCCCCACGGTGCGGGGGTTCCTCCGGGCAGGAAGGCGGGGGCCGACCCTGCTGTCCGCA
TCGCAGCGAGGAGCCGCAGCCACGCACCCCCCCGCACGCGCAGTGGGGGATTCGGCGAAGGAAGCTCGTAACATGGCGG
ACACCGAGGAAGGCTTCGGGCTCCCGAGCACGCCGGTTGACTCGGAGGCCAAGGAGCTGCAGGCTGAGGCCAAGCAGGA
TCCCCAGCTGGGGACCACCAGCAAGGCCCCCACCTCTCCACAGGCGGCCTTCACCCAGCAGGGCATGGAGGGGATCAAA
GTGTTTTTGCACGAGCGGGAGCTGTGGCTGAAATTTCACGAGGTGGGGACGGAGATGATCATAACAAAGGCTGGAAGGC
GTATGTTTCCCAGTTACAAAGTGAAGGTCACTGGACTCAATCCAAAAACGAAGTACATACTGTTGATGGATATTGTACC
AGCGGATGACCACAGATACAAATTTGCAGATAATAAATGGTCCGTGACCGGGAAGGCAGAACCGGCCATGCCCGGCCGC
CTCTACGTGCACCCCGACTCCCCCGCTACTGGAGCCCACTGGATGAGGCAGTTGGTTTCCTTCCAGAAGCTCAAGCTCA
CCAACAACCACCTTGACCCCTTCGGACATATCATCCTGAACTCCATGCACAAATACCAGCCCCGGCTCCACATCGTGAA
GGCGGATGAGAACAACGGCTTTGGCTCCAAGAACACTGCCTTCTGCACCCATGTCTTCCCCGAGACTGCCTTCATCGCT
GTTACCTCCTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTCGCAAAAGGTTTCCGCGGCAGCG
ATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACAGAA
AGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTACCAG
TGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCGCCCACCAACCCCTACCCGATCTCCCAGGAGCACAGCC
AGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGAAAC
TTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACTGAA
TCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCAGCT
GTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTACCA
GCATTTCTCTGCCCACTTCACCTCGGGGCCTCTGATGCCCCGGCTCAGCAGCGTGGCCAACCACACGTCCCCCCAGATA
GGAGACACCCATAGCATGTTCCAGCACCAGACCTCAGTTTCTCACCAACCCATTGTGCGGCAGTGTGGACCTCAGACCG
GCATCCAGTCTCCCCCCAGCAGCTTGCAGCCTGCAGAGTTCCTCTATTCCCACGGCGTGCCTCGAACCCTCTCGCCCCA
CCAGTACCACTCGGTGCACGGTGTGGGCATGGTGCCAGAGTGGAGCGAGAACAGCTAACGAGGCAGTCGATGGAAATGG
GAAAAAAAATAAAACGAAATGAAAGAAAAAAGTGAAGGGGGAAATAAGAAAAAAGGAAAGGGAAAACAAAACAAAACAA
AACAAAAAACCAGCACCCCATCAATAACAAAAACGAGAGCGTTTTGCAAGTCTCCTTTAACCGAATGTTCACCGTCAGC
ACTCGAGAGGGATGGACGCTGACCGTGCCACTGGTTGGAAGCACGGATGGAGCACTCATTGGCCATGGCACGATGTGCC
CCCCCCGGTGCTTTGGTTCAGAAGGCAAACCCAGCAGAGCGGAGCGCTGCCCCCACGTCCCCCCAGTCTGTGTCGCTGC
CACAGCCATCCCCTGCCTGGCCCTCCCCTGCCCCTCTCCTTCCCAAAGGGACTCGCGACTCCTGCTGCCTTCCTCAACA
CAGCCAGAGTGCTGGGATGAAAACAACTAGGATGTTAC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
25-30
stage 14

Eye

Quail

31-44
stage 44

Eye

Fig. 2. All images for comparison.

Quail

Probe 8

GEISHA IdTbx5.Plageman.2005
 NCBIEnsembl
Gene NameTBX5TBX5_CHICK
AliasesTBX5, T-box 5, Official 
Gene DescriptionT-box 5T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003700 transcription factor activity ISS
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005634 nucleus ISS
GO:0005737 cytoplasm ISS
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008134 transcription factor binding IEA
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter IEA
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030336 negative regulation of cell migration ISS
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0055007 cardiac muscle cell differentiation IEA
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
show
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
NCBI Nucleotide HitNM_204173 show details
scoreevaluealignment lengthdescription
21710.02171Gallus gallus T-box 5 (TBX5), mRNA
Ensembl Genome Map
contig view
CitationPlageman TF, Yutzey KE. T-Box genes and heart development: Putting the "T" in HearT. Dev Dyn. 2005 Jan;232(1):11-20.
CopyrightCopyright © 2005 Wiley-Liss, Inc.
Complete Sequenceshow
CGGGGGGCTGCCGCCATGCGGCCCCACGGTGCGGGGGTTCCTCCGGGCAGGAAGGCGGGGGCCGACCCTGCTGTCCGCA
TCGCAGCGAGGAGCCGCAGCCACGCACCCCCCCGCACGCGCAGTGGGGGATTCGGCGAAGGAAGCTCGTAACATGGCGG
ACACCGAGGAAGGCTTCGGGCTCCCGAGCACGCCGGTTGACTCGGAGGCCAAGGAGCTGCAGGCTGAGGCCAAGCAGGA
TCCCCAGCTGGGGACCACCAGCAAGGCCCCCACCTCTCCACAGGCGGCCTTCACCCAGCAGGGCATGGAGGGGATCAAA
GTGTTTTTGCACGAGCGGGAGCTGTGGCTGAAATTTCACGAGGTGGGGACGGAGATGATCATAACAAAGGCTGGAAGGC
GTATGTTTCCCAGTTACAAAGTGAAGGTCACTGGACTCAATCCAAAAACGAAGTACATACTGTTGATGGATATTGTACC
AGCGGATGACCACAGATACAAATTTGCAGATAATAAATGGTCCGTGACCGGGAAGGCAGAACCGGCCATGCCCGGCCGC
CTCTACGTGCACCCCGACTCCCCCGCTACTGGAGCCCACTGGATGAGGCAGTTGGTTTCCTTCCAGAAGCTCAAGCTCA
CCAACAACCACCTTGACCCCTTCGGACATATCATCCTGAACTCCATGCACAAATACCAGCCCCGGCTCCACATCGTGAA
GGCGGATGAGAACAACGGCTTTGGCTCCAAGAACACTGCCTTCTGCACCCATGTCTTCCCCGAGACTGCCTTCATCGCT
GTTACCTCCTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTCGCAAAAGGTTTCCGCGGCAGCG
ATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACAGAA
AGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTACCAG
TGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCGCCCACCAACCCCTACCCGATCTCCCAGGAGCACAGCC
AGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGAAAC
TTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACTGAA
TCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCAGCT
GTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTACCA
GCATTTCTCTGCCCACTTCACCTCGGGGCCTCTGATGCCCCGGCTCAGCAGCGTGGCCAACCACACGTCCCCCCAGATA
GGAGACACCCATAGCATGTTCCAGCACCAGACCTCAGTTTCTCACCAACCCATTGTGCGGCAGTGTGGACCTCAGACCG
GCATCCAGTCTCCCCCCAGCAGCTTGCAGCCTGCAGAGTTCCTCTATTCCCACGGCGTGCCTCGAACCCTCTCGCCCCA
CCAGTACCACTCGGTGCACGGTGTGGGCATGGTGCCAGAGTGGAGCGAGAACAGCTAACGAGGCAGTCGATGGAAATGG
GAAAAAAAATAAAACGAAATGAAAGAAAAAAGTGAAGGGGGAAATAAGAAAAAAGGAAAGGGAAAACAAAACAAAACAA
AACAAAAAACCAGCACCCCATCAATAACAAAAACGAGAGCGTTTTGCAAGTCTCCTTTAACCGAATGTTCACCGTCAGC
ACTCGAGAGGGATGGACGCTGACCGTGCCACTGGTTGGAAGCACGGATGGAGCACTCATTGGCCATGGCACGATGTGCC
CCCCCCGGTGCTTTGGTTCAGAAGGCAAACCCAGCAGAGCGGAGCGCTGCCCCCACGTCCCCCCAGTCTGTGTCGCTGC
CACAGCCATCCCCTGCCTGGCCCTCCCCTGCCCCTCTCCTTCCCAAAGGGACTCGCGACTCCTGCTGCCTTCCTCAACA
CAGCCAGAGTGCTGGGATGAAAACAACTAGGATGTTAC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
31-36
stage 36

Atria

Heart

Ventricles

atrial septum (as)

interventricular septum

Probe 9

GEISHA IdTbx5.Yamada.2000
 NCBIEnsembl
Gene NameTBX5TBX5_CHICK
AliasesTBX5, T-box 5, Official 
Gene DescriptionT-box 5T-box transcription factor TBX5 (T-box protein 5). [Source:Uniprot/SWISSPROT;Acc:Q9PWE8]
Gene Ontologyshow
GO:0002009 morphogenesis of an epithelium IEA
GO:0003700 transcription factor activity ISS
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005634 nucleus ISS
GO:0005737 cytoplasm ISS
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008134 transcription factor binding IEA
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter IEA
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030336 negative regulation of cell migration ISS
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0055007 cardiac muscle cell differentiation IEA
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
show
GO:0002009 morphogenesis of an epithelium IEA
GO:0003677 DNA binding IEA
GO:0003700 transcription factor activity IEA
GO:0003702 RNA polymerase II transcription factor activity ISS
GO:0005515 protein binding ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm ISS
GO:0006350 transcription IEA
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006917 induction of apoptosis ISS
GO:0007267 cell-cell signaling ISS
GO:0007389 pattern specification process IEA
GO:0007507 heart development ISS
GO:0008285 negative regulation of cell proliferation ISS
GO:0016563 transcription activator activity IEA
GO:0030324 lung development IEA
GO:0030326 embryonic limb morphogenesis ISS
GO:0030336 negative regulation of cell migration ISS
GO:0035115 embryonic forelimb morphogenesis IEA
GO:0035117 embryonic arm morphogenesis ISS
GO:0043565 sequence-specific DNA binding ISS
GO:0045449 regulation of transcription IEA
GO:0045893 positive regulation of transcription, DNA-dependent ISS
GO:0045941 positive regulation of transcription ISS
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0051891 positive regulation of cardioblast differentiation ISS
GO:0060039 pericardium development ISS
GO:0060044 negative regulation of cardiac muscle cell proliferation ISS
NCBI Nucleotide HitNM_204173 show details
scoreevaluealignment lengthdescription
13460.0695Gallus gallus T-box 5 (TBX5), mRNA
Ensembl Genome Map
contig view
CitationYamada M, Revelli JP, Eichele G, Barron M, Schwartz RJ. Expression of chick Tbx-2, Tbx-3, and Tbx-5 genes during early heart development: evidence for BMP2 induction of Tbx2. Dev Biol. 2000 Dec 1;228(1):95-105.
CopyrightReprinted with permission from Elsevier. Copyright © 2000 Elsevier B.V. All rights reserved.
CommentsInsufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204173).
Complete Sequenceshow
CGGGGGGCTGCCGCCATGCGGCCCCACGGTGCGGGGGTTCCTCCGGGCAGGAAGGCGGGGGCCGACCCTGCTGTCCGCA
TCGCAGCGAGGAGCCGCAGCCACGCACCCCCCCGCACGCGCAGTGGGGGATTCGGCGAAGGAAGCTCGTAACATGGCGG
ACACCGAGGAAGGCTTCGGGCTCCCGAGCACGCCGGTTGACTCGGAGGCCAAGGAGCTGCAGGCTGAGGCCAAGCAGGA
TCCCCAGCTGGGGACCACCAGCAAGGCCCCCACCTCTCCACAGGCGGCCTTCACCCAGCAGGGCATGGAGGGGATCAAA
GTGTTTTTGCACGAGCGGGAGCTGTGGCTGAAATTTCACGAGGTGGGGACGGAGATGATCATAACAAAGGCTGGAAGGC
GTATGTTTCCCAGTTACAAAGTGAAGGTCACTGGACTCAATCCAAAAACGAAGTACATACTGTTGATGGATATTGTACC
AGCGGATGACCACAGATACAAATTTGCAGATAATAAATGGTCCGTGACCGGGAAGGCAGAACCGGCCATGCCCGGCCGC
CTCTACGTGCACCCCGACTCCCCCGCTACTGGAGCCCACTGGATGAGGCAGTTGGTTTCCTTCCAGAAGCTCAAGCTCA
CCAACAACCACCTTGACCCCTTCGGACATATCATCCTGAACTCCATGCACAAATACCAGCCCCGGCTCCACATCGTGAA
GGCGGATGAGAACAACGGCTTTGGCTCCAAGAACACTGCCTTCTGCACCCATGTCTTCCCCGAGACTGCCTTCATCGCT
GTTACCTCCTACCAAAACCACAAGATCACTCAGCTGAAGATTGAGAACAACCCCTTCGCAAAAGGTTTCCGCGGCAGCG
ATGACATGGAGCTCCACAGGATGTCCAGGATGCAGAGTAAAGAGTACCCAGTTGTTCCCAGGAGCACAGTGAGACAGAA
AGTGTCCTCGAATCACAGCCCCTTCAGCGGTGAGACCAGGGTCCTTTCCACCTCCTCCAACCTGGGCTCCCAGTACCAG
TGTGAGAACGGGGTGTCAAGCACCTCCCAGGACCTGCTGCCGCCCACCAACCCCTACCCGATCTCCCAGGAGCACAGCC
AGATCTACCACTGCACCAAGAGAAAAGATGAGGAATGTTCCACCACCGAGCATCCCTACAAGAAGCCCTACATGGAAAC
TTCACCAGCGGAAGAGGATCCTTTCTACAGGTCCAGTTACCCCCAGCAACAGGGACTGAACACTTCGTACAGGACTGAA
TCAGCCCAGCGCCAGGCATGTATGTACGCCAGCTCTGCTCCCCCCACGGACCCCGTGCCCAGCCTGGAAGACATCAGCT
GTAACACGTGGCCCAGCGTGCCGTCCTACAGCAGTTGCACAGTGTCTGCCATGCAGCCCATGGACAGGTTACCCTACCA
GCATTTCTCTGCCCACTTCACCTCGGGGCCTCTGATGCCCCGGCTCAGCAGCGTGGCCAACCACACGTCCCCCCAGATA
GGAGACACCCATAGCATGTTCCAGCACCAGACCTCAGTTTCTCACCAACCCATTGTGCGGCAGTGTGGACCTCAGACCG
GCATCCAGTCTCCCCCCAGCAGCTTGCAGCCTGCAGAGTTCCTCTATTCCCACGGCGTGCCTCGAACCCTCTCGCCCCA
CCAGTACCACTCGGTGCACGGTGTGGGCATGGTGCCAGAGTGGAGCGAGAACAGCTAACGAGGCAGTCGATGGAAATGG
GAAAAAAAATAAAACGAAATGAAAGAAAAAAGTGAAGGGGGAAATAAGAAAAAAGGAAAGGGAAAACAAAACAAAACAA
AACAAAAAACCAGCACCCCATCAATAACAAAAACGAGAGCGTTTTGCAAGTCTCCTTTAACCGAATGTTCACCGTCAGC
ACTCGAGAGGGATGGACGCTGACCGTGCCACTGGTTGGAAGCACGGATGGAGCACTCATTGGCCATGGCACGATGTGCC
CCCCCCGGTGCTTTGGTTCAGAAGGCAAACCCAGCAGAGCGGAGCGCTGCCCCCACGTCCCCCCAGTCTGTGTCGCTGC
CACAGCCATCCCCTGCCTGGCCCTCCCCTGCCCCTCTCCTTCCCAAAGGGACTCGCGACTCCTGCTGCCTTCCTCAACA
CAGCCAGAGTGCTGGGATGAAAACAACTAGGATGTTAC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 5

Cardiovascular

Early Embryo

Cardiogenic Region

13-18
stage 8
stage 10

Heart Tube

 
19-20
stage 20
 

Figure 1 compares expression patterns of Tbx2, Tbx3, Tbx5 and bmp2 in early chick embryos. Figure includes HH stages 5-8, 10, 11, 16, 19 and 20.
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