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A chicken embryo gene expression database 		 
GEISHA - A Chicken Embryo Gene Expression Database
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Gene Expression Report

Probe 1

GEISHA IdWnt5b.Chapman.2004
 NCBIEnsembl
Gene NameWNT5BNP_001032346.1
AliasesWNT5B, wingless-type MMTV integration site family, member 5B, Official 
Gene Descriptionwingless-type MMTV integration site family, member 5Bwingless-type MMTV integration site family, member 5B [Source:RefSeq_peptide;Acc:NP_001032346]
Gene Ontologyshow
GO:0004871 signal transducer activity IEA
GO:0005515 protein binding IEA
GO:0005576 extracellular region IEA
GO:0005578 proteinaceous extracellular matrix IEA
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway IEA
GO:0007275 multicellular organismal development IEA
show
GO:0004871 signal transducer activity IEA
GO:0005515 protein binding ISS
GO:0005576 extracellular region IEA
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway IEA
GO:0007275 multicellular organismal development IEA
GO:0016055 Wnt receptor signaling pathway IEA
NCBI Nucleotide HitNM_001037269 show details
scoreevaluealignment lengthdescription
10920.01092Gallus gallus wingless-type MMTV integration site family, member 5B (WNT5B), mRNA
Ensembl Genome Map
contig view
CitationChapman, S, Brown, R, Lees, L, Schoenwolf, G, Lumsden, A. Expression Analysis of Chick Wnt and Frizzled Genes and Selected Inhibitors in Early Chick Patterning. Dev Dyn. 2004 Mar;229(3):668-76.
CopyrightWiley-Liss, Inc. 2004
Complete Sequenceshow
CACCATGACGGGGCCGAGGCTGGCGCTCGCCGCCGCGCTTCTCTGCAGCTGCACCTCGCCGGTGGCCGACGCCAACTCC
TGGTGGTCTTTGGCCATGAACCCCATCCAGAGGCCGGAGATGTACATCATCGGCGCGCAGCCCGTGTGCAGCCAGCTGC
CGGGGCTGTCCCCGGGGCAGCGCAAGCTGTGCCAGCTCTACCAGGAGCACATGGTGTTCATCGGCGAAGGGGCCCGCAG
TGCCATCAAGGAGTGCCAGTACCAGTTCCGGCAGCGCAGGTGGAACTGCAGCACGGTGGACAACACGTCCGTCTTCGGC
CGGGTCATGAAAATAGGTAGCCGAGAGACCGCTTTCACCTACGCCGTCAGCGCCGCCGGGGTGGTGAACGCCATCAGCC
GTGCCTGCCGTGAGGGAGAGCTGTCCAGCTGTGGCTGCAGCCGCACGGCCCGGCCCAAGGACTTGCCCCGAGACTGGCT
GTGGGGCGGCTGCGGGGATAACGTGGAGTACGGGTACCGTTTCGCCAAGGAGTTTGTGGATGCCAAGGAGAGGGAGAAG
AACTACGTGAGAGGCTCCGAGGAGCAGGCTCGCATGCTGATGAACCTGCAGAACAACGAGGCTGGGCGCAGGGCCGTGT
ACAAGCTGGCGGACGTGGCCTGCAAGTGCCACGGCGTGTCGGGCTCCTGCAGCCTCAAGACGTGCTGGCTGCAGCTGGC
AGACTTCCGCAAGGTGGGCGACCTGCTGAAGGAGAAGTACGACAGCGCGGCCGCCATGCGGATCAGTCGCAAGGGCAAG
CTGGAGCTCGTGAACAACCGCTTCAACATGCCCACGCAGGAGGACCTGGTCTACGTCGACCCCAGCCCGGACTATTGCC
TGCGCAATGAGACCACCGGCTCGCTGGGCACGCAGGGCCGCCTGTGCAACAAGACCTCAGAGGGCATGGATGGGTGTGA
GCTGATGTGCTGTGGACGGGGCTACGACCAATTCAAGAGCGTTCAGGTGGAGCGTTGCCACTGCAAGTTCCATTGGTGC
TGTTACGTCAAGTGTAAAAAGTGCACAGAGATCGTTGACCAGTACGTCTGTAAATGAAAAGAGCC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-5
stage 4
stage 4
stage 5

Primitive Streak

 

Probe 2

GEISHA IdWnt5b.Fokina.2006
 NCBIEnsembl
Gene NameWNT5BNP_001032346.1
AliasesWNT5B, wingless-type MMTV integration site family, member 5B, Official 
Gene Descriptionwingless-type MMTV integration site family, member 5Bwingless-type MMTV integration site family, member 5B [Source:RefSeq_peptide;Acc:NP_001032346]
Gene Ontologyshow
GO:0004871 signal transducer activity IEA
GO:0005515 protein binding IEA
GO:0005576 extracellular region IEA
GO:0005578 proteinaceous extracellular matrix IEA
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway IEA
GO:0007275 multicellular organismal development IEA
show
GO:0004871 signal transducer activity IEA
GO:0005515 protein binding ISS
GO:0005576 extracellular region IEA
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway IEA
GO:0007275 multicellular organismal development IEA
GO:0016055 Wnt receptor signaling pathway IEA
NCBI Nucleotide HitNM_001037269 show details
scoreevaluealignment lengthdescription
10920.01092Gallus gallus wingless-type MMTV integration site family, member 5B (WNT5B), mRNA
Ensembl Genome Map
contig view
CitationFokina VM, Frolova EI. Expression patterns of Wnt genes during development of an anterior part of the chicken eye. Dev Dyn. 2006 Feb;235(2):496-505.
CopyrightCopyright © 2008 Wiley-Liss, Inc.
Complete Sequenceshow
CACCATGACGGGGCCGAGGCTGGCGCTCGCCGCCGCGCTTCTCTGCAGCTGCACCTCGCCGGTGGCCGACGCCAACTCC
TGGTGGTCTTTGGCCATGAACCCCATCCAGAGGCCGGAGATGTACATCATCGGCGCGCAGCCCGTGTGCAGCCAGCTGC
CGGGGCTGTCCCCGGGGCAGCGCAAGCTGTGCCAGCTCTACCAGGAGCACATGGTGTTCATCGGCGAAGGGGCCCGCAG
TGCCATCAAGGAGTGCCAGTACCAGTTCCGGCAGCGCAGGTGGAACTGCAGCACGGTGGACAACACGTCCGTCTTCGGC
CGGGTCATGAAAATAGGTAGCCGAGAGACCGCTTTCACCTACGCCGTCAGCGCCGCCGGGGTGGTGAACGCCATCAGCC
GTGCCTGCCGTGAGGGAGAGCTGTCCAGCTGTGGCTGCAGCCGCACGGCCCGGCCCAAGGACTTGCCCCGAGACTGGCT
GTGGGGCGGCTGCGGGGATAACGTGGAGTACGGGTACCGTTTCGCCAAGGAGTTTGTGGATGCCAAGGAGAGGGAGAAG
AACTACGTGAGAGGCTCCGAGGAGCAGGCTCGCATGCTGATGAACCTGCAGAACAACGAGGCTGGGCGCAGGGCCGTGT
ACAAGCTGGCGGACGTGGCCTGCAAGTGCCACGGCGTGTCGGGCTCCTGCAGCCTCAAGACGTGCTGGCTGCAGCTGGC
AGACTTCCGCAAGGTGGGCGACCTGCTGAAGGAGAAGTACGACAGCGCGGCCGCCATGCGGATCAGTCGCAAGGGCAAG
CTGGAGCTCGTGAACAACCGCTTCAACATGCCCACGCAGGAGGACCTGGTCTACGTCGACCCCAGCCCGGACTATTGCC
TGCGCAATGAGACCACCGGCTCGCTGGGCACGCAGGGCCGCCTGTGCAACAAGACCTCAGAGGGCATGGATGGGTGTGA
GCTGATGTGCTGTGGACGGGGCTACGACCAATTCAAGAGCGTTCAGGTGGAGCGTTGCCACTGCAAGTTCCATTGGTGC
TGTTACGTCAAGTGTAAAAAGTGCACAGAGATCGTTGACCAGTACGTCTGTAAATGAAAAGAGCC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
25-30
stage 19
stage 19
stage 23

Eye

Lens

Retina

 
31
stage 31

Eye

 

Probe 3

GEISHA IdWnt5b.Hardy.2008
 NCBIEnsembl
Gene Name NP_001032346.1
Gene Description wingless-type MMTV integration site family, member 5B [Source:RefSeq_peptide;Acc:NP_001032346]
Gene Ontologyshow
GO:0004871 signal transducer activity IEA
GO:0005515 protein binding ISS
GO:0005576 extracellular region IEA
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway IEA
GO:0007275 multicellular organismal development IEA
GO:0016055 Wnt receptor signaling pathway IEA
Ensembl Genome Map
contig view
CitationHardy KM, Garriock RJ, Yatskievych TA, D'Agostino SL, Antin PB, Krieg PA. Non-canonical Wnt signaling through Wnt5a/b and a novel Wnt11 gene, Wnt11b, regulates cell migration during avian gastrulation. Dev Biol. 2008 Aug 15;320(2):391-401. Epub 2008 May 29.
Copyright"Reprinted from Developmental Biology, 320, Hardy, KM, Garriock, RJ, Yatskievych, TA, D'Agostino, SL, Antin, PB, Krieg, PA. Non-canonical Wnt signaling through Wnt5a/b and a novel Wnt11 gene, Wnt11b, regulates cell migration during avian gastrulation., Pages No., Copyright (2008), with permission from Elsevier". Copyright © 2008 Elsevier B.V. All rights reserved.
CommentsThe authors said: Wnt5a (Accession # AB006014) and Wnt5b (Accession # AY753289) templates were prepared by linearizing with EcoRI and transcribing with T3 polymerase. AY753289= NM_001037269.1
Complete Sequenceshow
CACCATGACGGGGCCGAGGCTGGCGCTCGCCGCCGCGCTTCTCTGCAGCTGCACCTCGCCGGTGGCCGACGCCAACTCC
TGGTGGTCTTTGGCCATGAACCCCATCCAGAGGCCGGAGATGTACATCATCGGCGCGCAGCCCGTGTGCAGCCAGCTGC
CGGGGCTGTCCCCGGGGCAGCGCAAGCTGTGCCAGCTCTACCAGGAGCACATGGTGTTCATCGGCGAAGGGGCCCGCAG
TGCCATCAAGGAGTGCCAGTACCAGTTCCGGCAGCGCAGGTGGAACTGCAGCACGGTGGACAACACGTCCGTCTTCGGC
CGGGTCATGAAAATAGGTAGCCGAGAGACCGCTTTCACCTACGCCGTCAGCGCCGCCGGGGTGGTGAACGCCATCAGCC
GTGCCTGCCGTGAGGGAGAGCTGTCCAGCTGTGGCTGCAGCCGCACGGCCCGGCCCAAGGACTTGCCCCGAGACTGGCT
GTGGGGCGGCTGCGGGGATAACGTGGAGTACGGGTACCGTTTCGCCAAGGAGTTTGTGGATGCCAAGGAGAGGGAGAAG
AACTACGTGAGAGGCTCCGAGGAGCAGGCTCGCATGCTGATGAACCTGCAGAACAACGAGGCTGGGCGCAGGGCCGTGT
ACAAGCTGGCGGACGTGGCCTGCAAGTGCCACGGCGTGTCGGGCTCCTGCAGCCTCAAGACGTGCTGGCTGCAGCTGGC
AGACTTCCGCAAGGTGGGCGACCTGCTGAAGGAGAAGTACGACAGCGCGGCCGCCATGCGGATCAGTCGCAAGGGCAAG
CTGGAGCTCGTGAACAACCGCTTCAACATGCCCACGCAGGAGGACCTGGTCTACGTCGACCCCAGCCCGGACTATTGCC
TGCGCAATGAGACCACCGGCTCGCTGGGCACGCAGGGCCGCCTGTGCAACAAGACCTCAGAGGGCATGGATGGGTGTGA
GCTGATGTGCTGTGGACGGGGCTACGACCAATTCAAGAGCGTTCAGGTGGAGCGTTGCCACTGCAAGTTCCATTGGTGC
TGTTACGTCAAGTGTAAAAAGTGCACAGAGATCGTTGACCAGTACGTCTGTAAATGAAAAGAGCC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 4
stage 4

Early Mesoderm

Epiblast

Primitive Streak

 
7-12
stage 7
stage 11

Early Mesoderm

Epiblast

Primitive Streak

 
13
stage 13
 

Fig. 2 compares expression for several Wnts at several stages.

Probe 4

GEISHA IdWnt5b.Chapman.2008
 NCBIEnsembl
Gene Name NP_001032346.1
Gene Description wingless-type MMTV integration site family, member 5B [Source:RefSeq_peptide;Acc:NP_001032346]
Gene Ontologyshow
GO:0004871 signal transducer activity IEA
GO:0005515 protein binding ISS
GO:0005576 extracellular region IEA
GO:0007223 Wnt receptor signaling pathway, calcium modulating pathway IEA
GO:0007275 multicellular organismal development IEA
GO:0016055 Wnt receptor signaling pathway IEA
Ensembl Genome Map
contig view
CommentsImages Provided by Susan Chapman.
Complete Sequenceshow
CACCATGACGGGGCCGAGGCTGGCGCTCGCCGCCGCGCTTCTCTGCAGCTGCACCTCGCCGGTGGCCGACGCCAACTCC
TGGTGGTCTTTGGCCATGAACCCCATCCAGAGGCCGGAGATGTACATCATCGGCGCGCAGCCCGTGTGCAGCCAGCTGC
CGGGGCTGTCCCCGGGGCAGCGCAAGCTGTGCCAGCTCTACCAGGAGCACATGGTGTTCATCGGCGAAGGGGCCCGCAG
TGCCATCAAGGAGTGCCAGTACCAGTTCCGGCAGCGCAGGTGGAACTGCAGCACGGTGGACAACACGTCCGTCTTCGGC
CGGGTCATGAAAATAGGTAGCCGAGAGACCGCTTTCACCTACGCCGTCAGCGCCGCCGGGGTGGTGAACGCCATCAGCC
GTGCCTGCCGTGAGGGAGAGCTGTCCAGCTGTGGCTGCAGCCGCACGGCCCGGCCCAAGGACTTGCCCCGAGACTGGCT
GTGGGGCGGCTGCGGGGATAACGTGGAGTACGGGTACCGTTTCGCCAAGGAGTTTGTGGATGCCAAGGAGAGGGAGAAG
AACTACGTGAGAGGCTCCGAGGAGCAGGCTCGCATGCTGATGAACCTGCAGAACAACGAGGCTGGGCGCAGGGCCGTGT
ACAAGCTGGCGGACGTGGCCTGCAAGTGCCACGGCGTGTCGGGCTCCTGCAGCCTCAAGACGTGCTGGCTGCAGCTGGC
AGACTTCCGCAAGGTGGGCGACCTGCTGAAGGAGAAGTACGACAGCGCGGCCGCCATGCGGATCAGTCGCAAGGGCAAG
CTGGAGCTCGTGAACAACCGCTTCAACATGCCCACGCAGGAGGACCTGGTCTACGTCGACCCCAGCCCGGACTATTGCC
TGCGCAATGAGACCACCGGCTCGCTGGGCACGCAGGGCCGCCTGTGCAACAAGACCTCAGAGGGCATGGATGGGTGTGA
GCTGATGTGCTGTGGACGGGGCTACGACCAATTCAAGAGCGTTCAGGTGGAGCGTTGCCACTGCAAGTTCCATTGGTGC
TGTTACGTCAAGTGTAAAAAGTGCACAGAGATCGTTGACCAGTACGTCTGTAAATGAAAAGAGCC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
1-3
stage 3

Primitive Streak

 
4-6
stage 4
stage 4
stage 4
stage 5
stage 5
stage 5
stage 5

Area Pellucida

Epiblast

Neural Plate/Tube

Primitive Streak

 
7-10
stage 8
stage 8
stage 10
stage 10
stage 10
stage 10

Anterior Intestinal Portal

Epiblast

Forebrain

Head Mesenchyme

Midbrain

Neural Plate/Tube

Paraxial Mesoderm

Primitive Streak

Somites

Trunk Mesenchyme

 
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