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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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HAS2
hyaluronan synthase 2

Gene

Official Symbol	HAS2
Official Full Name	hyaluronan synthase 2
CGNC ID	49391
also known as	hyaluronan synthase 2\|HA synthase 2\|hyaluronate synthase 2\|hyaluronic acid synthase 2, Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, CHAS2
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_204806
Polypeptide	NP_990137

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	3037	ENSG00000170961		MIM:601636
Mouse	15117	ENSMUSG00000022367	All phenotypic alleles (7):Targeted, knock-out(2) Targeted, other(5)	MGI:107821
Xenopus	100145711, 399146			5775580
Zebrafish	260350	ENSDARG00000036987	ZDB-GENE-020828-1	ZFIN:ZDB-GENE-020828-1

Gene Ontology

Molecular Function	hyaluronan synthase activity
Biological Process	atrioventricular canal development, endocardial cushion to mesenchymal transition, more...
Cellular Component	integral component of plasma membrane

Links to other databases

Entrez Gene	395594
Ensembl Gene
KEGG	gga:395594

Entry 1

GEISHA Id	V47	Data Source	GEISHA ISH Analysis
Forward cDNA Template Probe	show GAGTCCTATTTTGGCTGCGTACAGTGCATCAGTGGACCTCTGGGAATGTACAGAAACTCTTTACTCCATGAATTTGTGG AAGATTGGTACAATCAAGAATTTATGGGCTCCCAGTGCAGCTTTGGAGACGACAGGCATCTAACTAACCGAGTGCTAAG TCTGGGCTATGCAACAAAATACACAGCTAGATCCAAGTGCCTTACTGAAACACCAATAGAGTATCTCAGGTGGCTGAAT CAGCAGACCCGCTGGAGTAAATCGTATTTTAGAGAGTGGCTTTATAATGCAATGTGGTTCCACAAGCACCATTTGTGGA TGACCTATGAAGCTGTAATCACTGGATTCTTTCCTTTCTTCCTTATCGCTACAGTCATTCAGCTCTTCTACAGGGGAAA AATCTGGAACATCCTCCTTTTCTTGTTGACAGTTCAGTTAGTGGGCCTGATAAAGTCTTCCTTTGCCAGTTTCCTTAGG GGCAACATTGTCATGGTTTTCATGTCACTCTACTCAGTGTTGTATATGTCGAGTTT
Reverse cDNA Template Probe	show GACCTTGTAGTATCTGTGGCGTGGGACGAATGTTGCCAAGGGAAATGGATCACTGCTGCTGGGAATAGGACATTTATAT TCCTCCGGGTTCATTTTCATCCTGCCAAAGTAAGAAAATCAAAACCAATAGAAAATGATTTTTTTTTCCAAGGGGGGAA AAATCAAACCACACCGTTTCAACCTGTTCGCATGAAAATGGGAGAACTTCTTTGTTTATGCACTTTTTTATTGTGCATA CGCCTGACAGTGTTACGTTCTATATACCTCACTGGTCATGCTTATGTGTGGACAGGAAGAAAAGGAGTTTGGAGAATCA AGAAAAGGATCTTACAACCCCTTGCAACCTAATTTATGAACTTCTCTTTTTTATAGTTCTTTTTATAGGAAGGGTCTTT TGTGTTAGGCTGCCAAATGTAATGCCAAAGGTAAATTTTAAGAGGCCATTGGCTGAGCTGTATTTTTATATTATTGTAT TATTTGTGTGTGTGTATTTTTAAGCCAACTTTTTTTTTTTTTTTAAATCACATATTTTATATTTTACTATCTGCCAAAA AAAAAAAAAAAAAA

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 6

Heart

7-12

stage 8

stage 9

stage 11

stage 12

Ear/Otic Placcode
Heart
Tail

Entry 2

GEISHA Id	Has2.Klewer.2006	Data Source	Publication
Complete cDNA Template Probe	show TGCAATCGCCACGATAAACAAAGCAGGGTGGGGCACATCAGGAAGAAAAACCATTGTAGTTAATTTTATAGGACTCATT CCAGTCTCCATTTGGTTTACAATCCTCCTAGGTCGCGTAATTTTCACTATCTACAAGGAATCAAAAAAGCCATTCTCTG AGTCAAAAACAACAGTTCTCGTCATTGGTACAATCCTCTATGCATGTTACTGGGTTCTTCTATTGACTTTGTACTTGGT TCTCATCACCAAATGTGGCAGGCGGAAGAAAGAGCAACACTATGACATGGTGCTAGATGTATGATGTTTCTGTGGGAAG TTACCCAGAGAGTTTTAAAGCAACCCAAGGACCTTGTAGTATCTGTGGCATGGGACGAATGTTGCCAAGGGAAATGGAT CACTGCTGCTGGAATAGGACATTTATATTCTCCGGGTCCATTTTCATCCTGCCAAAGTAAGAAAATCAAAAACGATAGA AAATGATTTTTTTTTCCAAGGGGGGAAAAATCAAACCACACCGTTTCAACCTGTTCGGATGAAAATGGGAGAACTTCTT TGTTTATGCACTTTTTTATTGTCGATACGCCTGACAGTGTTACGTTCTATATACCTCACTGGTCATGCTTATGTGTGGA CAGGAAGAAAAGGAGTTTGGAGAATCAAGAAAAGGATCTTACAACCCCTTGCAACCTAATTTATGAACTTCTCTTTTTT ATAGTTCTTTTTATAGGAAGGGTCTTTTGTGTTAGGCTGCCAAATGTAATGCCAAAGGGAAATCTTAAGAGCGATTGGC TGAGG
Citation	Klewer SE, Yatskievych T, Pogreba K, Stevens MV, Antin PB, Camenisch TD. Has2 expression in heart forming regions is independent of BMP signaling. Gene Expr Patterns. 2006 Jun;6(5):462-70. Epub 2006 Feb 3.
Copyright	Reprinted with permission from Elsevier. Copyright © 2005 Elsevier B.V. All rights reserved.
Comments	Digoxigenin (DIG)-labeled antisense riboprobes were generated from linearized cDNA clones for chicken Has2 (bp 19212715; Genbank # AF106940).

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 6

Area Pellucida
Heart
Hensen's Node
Lateral Plate Mesoderm
Primitive Streak

anterior
cardiac crescent

7-12

stage 7

stage 8

stage 10

Heart
Heart Tube
Lateral Plate Mesoderm
Primitive Streak
Splanchnic Mesoderm
Tail

cardiac crescent
cardiogenic/hepatogenic crescent

13-18

stage 14

stage 15

stage 18

Atria
Ear/Otic Placcode
Endocardium
Heart Tube
Myocardium
Outflow Tract
Pharyngeal Arches and Clefts
Proepicardium
Somites
Surface Ectoderm
Ventricles

cushions

19-24

stage 20

stage 24

Atria
Ear/Otic Placcode
Endocardium
Outflow Tract
Pharyngeal Arches and Clefts
Surface Ectoderm
Ventricles

AV canal
cushions

Entry 3

GEISHA Id	Has2.Li.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGGAAAGGAACAGACGTCTGGAAAATATTACCCGTTTGCTGCTGCTGCTGCTGAGAACTAAAGCAGCTACGCGTCCGC AGGCATCACCAAAGAGCCTTTATCTGATGCTGCACATCCCAAACTTTCAGCCGCTGCCGTGCAACTCCTCCAGGCTCCT GCCAGCCGGGTTTGAAACGAGGTCTTCAGCTTCGACCACGAGAAACTTAACTCCTAATTTATTATTTCTAACTTTTTTT TTTTTAATTATTATTATTATTTGAAAAAAAAAAAAAAAGAAAAAAATCCCACGACTTCGAGCTAGAAACTGAAGACAAA TACATTGTAATAGGGAAAACACGTGGGAGAAAATGAATAGGACTGAATAGCCTTAAGCGATTTTTTTTATTATTTTCAT TTTTTTTAATTATTTTTTTTTATTCCTGAGCTTCTGAATAAGTCCCTGAAAAAGTGCGATTTGATCTTTTACCTCAAAC TTGGAAACTGTTCAGCGGGAGAACGGTACGTGAGACCACACAGCTGGCTGGAGCCCCTCTATGACCAAAGGACTGCCGG ACAAACAAGATGTATTGTGAGAGGTTTATATGTATCCTGAGAATACTTGGAACCACACTCTTTGGAGTGTCCCTCCTGC TGGGAATCACCGCTGCTTACATTGTGGGCTACCAGTTTATCCAAACAGACAATTACTACTTCTCCTTTGGACTCTATGG TGCTATCCTGGCATCACATCTCATCATCCAAAGCCTGTTTGCCTACCTAGAGCACAGGAAAATGAAGCGGTCGCTAGAG ACTCCAATCAAGCTGAACAAAACTGTTGCCCTTTGTATTGCTGCCTATCAAGAGGATCCTGACTACTTAAGAAAATGTT TACTTTCTGTGAAAAGATTGACCTACCCTGGAATTAAAGTTGTTATGGTCATTGATGGGAACTCAGAAGATGACGTTTA CATGATGGACATTTTTACTGAAATCATGGGGAGGGACAAATCTGCCACTTATATCTGGAGTAACAACTTCCATGACAAA GGTCCAGGTGAGACGGAGGAGTCTCACAGAGAGAGCATGCAGCACGTATCTCAGCTGGTCCTGTCCAACAAAAGTGTTT GCATCATGCAGAAATGGGGTGGAAAAAGAGAAGTAATGTACACAGCATTCAAAGCACTGGGAGAAGCGTGGAATTATGT ACAGGTCTGTGATTCAGACACAATGCTCGATCCAGCTTCATCAGTGGAAATGGTAAAGGTCTTAGAAGAAGATCCAATG GTTGGAGGAGTTGGAGGCGATGTGCAGATTTTGAACAAGTATGATTCCTGGATCTCCTTTCTGAGCAGTGTGAGATACT GGATGGCATTTAACATAGAAAGAGCCTGCCAGTCCTATTTTGGCTGCGTACAGTGCATCAGTGGACCTCTGGGAATGTA CAGAAACTCTTTACTCCATGAATTTGTGGAAGATTGGTACAATCAAGAATTTATGGGCTCCCAGTGCAGCTTTGGAGAC GACAGGCATCTAACTAACCGAGTGCTAAGTCTGGGCTATGCAACAAAATACACAGCTAGATCCAAGTGCCTTACTGAAA CACCAATAGAGTATCTCAGGTGGCTGAATCAGCAGACCCGCTGGAGTAAATCGTATTTTAGAGAGTGGCTTTATAATGC AATGTGGTTCCACAAGCACCATTTGTGGATGACCTATGAAGCTGTAATCACTGGATTCTTTCCTTTCTTCCTTATCGCT ACAGTCATTCAGCTCTTCTACAGGGGAAAAATCTGGAACATCCTCCTTTTCTTGTTGACAGTTCAGTTAGTGGGCCTGA TAAAGTCTTCCTTTGCCAGTTTCCTTAGGGGCAACATTGTCATGGTTTTCATGTCACTCTACTCAGTGTTGTATATGTC GAGTTTACTGCCAGCAAAGATGTTTGCAATCGCCACGATAAACAAAGCAGGGTGGGGCACATCAGGAAGAAAAACCATT GTAGTTAATTTTATAGGACTCATTCCAGTCTCCATTTGGTTTACAATCCTCCTAGGTCGCGTAATTTTCACTATCTACA AGGAATCAAAAAAGCCATTCTCTGAGTCAAAAACAACAGTTCTCGTCATTGGTACAATCCTCTATGCATGTTACTGGGT TCTTCTATTGACTTTGTACTTGGTTCTCATCACCAAATGTGGCAGGCGGAAGAAAGAGCAACACTATGACATGGTGCTA GATGTATGATGTTTCTGTGGGAAGTTACCCAGAGAGTTTTAAAGCAACCCAAGGACCTTGTAGTATCTGTGGCATGGGA CGAATGTTGCCAAGGGAAATGGATCACTGCTGCTGGAATAGGACATTTATATTCTCCGGGTCCATTTTCATCCTGCCAA AGTAAGAAAATCAAAAACGATAGAAAATGATTTTTTTTTCCAAGGGGGGAAAAATCAAACCACACCGTTTCAACCTGTT CGGATGAAAATGGGAGAACTTCTTTGTTTATGCACTTTTTTATTGTCGATACGCCTGACAGTGTTACGTTCTATATACC TCACTGGTCATGCTTATGTGTGGACAGGAAGAAAAGGAGTTTGGAGAATCAAGAAAAGGATCTTACAACCCCTTGCAAC CTAATTTATGAACTTCTCTTTTTTATAGTTCTTTTTATAGGAAGGGTCTTTTGTGTTAGGCTGCCAAATGTAATGCCAA AGGGAAATCTTAAGAGCGATTGGCTGAGGCGTATTTTTATATTATTGTATTATTTGTGTGTGTGTATTTTTAAGCGAAC TTTTTTTTTTTCAAATCACATATTTTATATTTTACTATCTGCGGG
Citation	Li Y, Toole BP, Dealy CN, Kosher RA. Hyaluronan in limb morphogenesis. Dev Biol. 2007 May 15;305(2):411-20. Epub 2007 Feb 24.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # AF106940.1).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Leg Mesenchyme
Wing Mesenchyme

19-24

stage 20

Leg AER
Leg Mesenchyme
Wing AER
Wing Mesenchyme

25-27

stage 25

stage 27

Leg Mesenchyme
Wing Mesenchyme

Entry 4

GEISHA Id	HAS2.Roddy.2011	Data Source	Publication
Complete cDNA Template Probe	show `TCCTAGGTCGCGTAATTTTCACTATCTACAAGGAATCAAAAAAGCCATTCTCTGAGTCAAAAACAACAGTTCTCGTCAT TGGTACAATCCTCTATGCATGTTACTGGGTTCTTCTATTGACTTTGTACTTGGTTCTCATCACCAAATGTGGCAGGCGG AAGAAAGAGCAACACTATGACATGGTGCTAGATGTATGATGTTTCTGTGGGAAGTTACCCAGAGAGTTTTAAAGCAACC CAAGGACCTTGTAGTATCTGTGGCATGGGACGAATGTTGCCAAGGGAAATGGATCACTGCTGCTGGAATAGGACATTTA TATTCTCCGGGTCCATTTTCATCCTGCCAAAGTAAGAAAATCAAAAACGATAGAAAATGATTTTTTTTTCCAAGGGGGG AAAAATCAAACCACACCGTTTCAACCTGTTCGGATGAAAATGGGAGAACTTCTTTGTTTATGCACTTTTTTATTGTCGA TACGCCTGA`
Citation	Roddy KA, Prendergast PJ, Murphy P. Mechanical influences on morphogenesis of the knee joint revealed through morphological, molecular and computational analysis of immobilised embryos. PLoS One. 2011 Feb 28;6(2):e17526.
Copyright	PLoS One is an open access journal.
Comments	The cDNA template used to generate the probe was obtained from EST - ChEST500e4 as described in Roddy et al.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-27

stage 27

Leg

il, intermediate layer

Entry 5

GEISHA Id	HAS2.Song.2011	Data Source	Publication
Complete cDNA Template Probe	show CAGGAAAGGAACAGACGTCTGGAAAATATTACCCGTTTGCTGCTGCTGCTGCTGAGAACTAAAGCAGCTACGCGTCCGC AGGCATCACCAAAGAGCCTTTATCTGATGCTGCACATCCCAAACTTTCAGCCGCTGCCGTGCAACTCCTCCAGGCTCCT GCCAGCCGGGTTTGAAACGAGGTCTTCAGCTTCGACCACGAGAAACTTAACTCCTAATTTATTATTTCTAACTTTTTTT TTTTTAATTATTATTATTATTTGAAAAAAAAAAAAAAAGAAAAAAATCCCACGACTTCGAGCTAGAAACTGAAGACAAA TACATTGTAATAGGGAAAACACGTGGGAGAAAATGAATAGGACTGAATAGCCTTAAGCGATTTTTTTTATTATTTTCAT TTTTTTTAATTATTTTTTTTTATTCCTGAGCTTCTGAATAAGTCCCTGAAAAAGTGCGATTTGATCTTTTACCTCAAAC TTGGAAACTGTTCAGCGGGAGAACGGTACGTGAGACCACACAGCTGGCTGGAGCCCCTCTATGACCAAAGGACTGCCGG ACAAACAAGATGTATTGTGAGAGGTTTATATGTATCCTGAGAATACTTGGAACCACACTCTTTGGAGTGTCCCTCCTGC TGGGAATCACCGCTGCTTACATTGTGGGCTACCAGTTTATCCAAACAGACAATTACTACTTCTCCTTTGGACTCTATGG TGCTATCCTGGCATCACATCTCATCATCCAAAGCCTGTTTGCCTACCTAGAGCACAGGAAAATGAAGCGGTCGCTAGAG ACTCCAATCAAGCTGAACAAAACTGTTGCCCTTTGTATTGCTGCCTATCAAGAGGATCCTGACTACTTAAGAAAATGTT TACTTTCTGTGAAAAGATTGACCTACCCTGGAATTAAAGTTGTTATGGTCATTGATGGGAACTCAGAAGATGACGTTTA CATGATGGACATTTTTACTGAAATCATGGGGAGGGACAAATCTGCCACTTATATCTGGAGTAACAACTTCCATGACAAA GGTCCAGGTGAGACGGAGGAGTCTCACAGAGAGAGCATGCAGCACGTATCTCAGCTGGTCCTGTCCAACAAAAGTGTTT GCATCATGCAGAAATGGGGTGGAAAAAGAGAAGTAATGTACACAGCATTCAAAGCACTGGGAGAAGCGTGGAATTATGT ACAGGTCTGTGATTCAGACACAATGCTCGATCCAGCTTCATCAGTGGAAATGGTAAAGGTCTTAGAAGAAGATCCAATG GTTGGAGGAGTTGGAGGCGATGTGCAGATTTTGAACAAGTATGATTCCTGGATCTCCTTTCTGAGCAGTGTGAGATACT GGATGGCATTTAACATAGAAAGAGCCTGCCAGTCCTATTTTGGCTGCGTACAGTGCATCAGTGGACCTCTGGGAATGTA CAGAAACTCTTTACTCCATGAATTTGTGGAAGATTGGTACAATCAAGAATTTATGGGCTCCCAGTGCAGCTTTGGAGAC GACAGGCATCTAACTAACCGAGTGCTAAGTCTGGGCTATGCAACAAAATACACAGCTAGATCCAAGTGCCTTACTGAAA CACCAATAGAGTATCTCAGGTGGCTGAATCAGCAGACCCGCTGGAGTAAATCGTATTTTAGAGAGTGGCTTTATAATGC AATGTGGTTCCACAAGCACCATTTGTGGATGACCTATGAAGCTGTAATCACTGGATTCTTTCCTTTCTTCCTTATCGCT ACAGTCATTCAGCTCTTCTACAGGGGAAAAATCTGGAACATCCTCCTTTTCTTGTTGACAGTTCAGTTAGTGGGCCTGA TAAAGTCTTCCTTTGCCAGTTTCCTTAGGGGCAACATTGTCATGGTTTTCATGTCACTCTACTCAGTGTTGTATATGTC GAGTTTACTGCCAGCAAAGATGTTTGCAATCGCCACGATAAACAAAGCAGGGTGGGGCACATCAGGAAGAAAAACCATT GTAGTTAATTTTATAGGACTCATTCCAGTCTCCATTTGGTTTACAATCCTCCTAGGTCGCGTAATTTTCACTATCTACA AGGAATCAAAAAAGCCATTCTCTGAGTCAAAAACAACAGTTCTCGTCATTGGTACAATCCTCTATGCATGTTACTGGGT TCTTCTATTGACTTTGTACTTGGTTCTCATCACCAAATGTGGCAGGCGGAAGAAAGAGCAACACTATGACATGGTGCTA GATGTATGATGTTTCTGTGGGAAGTTACCCAGAGAGTTTTAAAGCAACCCAAGGACCTTGTAGTATCTGTGGCATGGGA CGAATGTTGCCAAGGGAAATGGATCACTGCTGCTGGAATAGGACATTTATATTCTCCGGGTCCATTTTCATCCTGCCAA AGTAAGAAAATCAAAAACGATAGAAAATGATTTTTTTTTCCAAGGGGGGAAAAATCAAACCACACCGTTTCAACCTGTT CGGATGAAAATGGGAGAACTTCTTTGTTTATGCACTTTTTTATTGTCGATACGCCTGACAGTGTTACGTTCTATATACC TCACTGGTCATGCTTATGTGTGGACAGGAAGAAAAGGAGTTTGGAGAATCAAGAAAAGGATCTTACAACCCCTTGCAAC CTAATTTATGAACTTCTCTTTTTTATAGTTCTTTTTATAGGAAGGGTCTTTTGTGTTAGGCTGCCAAATGTAATGCCAA AGGGAAATCTTAAGAGCGATTGGCTGAGGCGTATTTTTATATTATTGTATTATTTGTGTGTGTGTATTTTTAAGCGAAC TTTTTTTTTTTCAAATCACATATTTTATATTTTACTATCTGCGGG
Citation	Song G, Seo HW, Choi JW, Rengaraj D, Kim TM, Lee BR, Kim YM, Yun TW, Jeong JW, Han JY. Discovery of candidate genes and pathways regulating oviduct development in chickens. Biol Reprod. 2011 Aug;85(2):306-14. Epub 2011 May 4.
Copyright	Copyright © 2012 by the Biology of Reproduction
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204806.1).