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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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WNT16
Wnt family member 16

Gene

Official Symbol	WNT16
Official Full Name	Wnt family member 16
CGNC ID	53210
also known as	protein Wnt-16\|wingless-type MMTV integration site family, member 16, WNT16
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC

Sequence Information

Genomic
RNA	XM_001233677
Polypeptide	XP_001233678

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	51384	ENSG00000002745		MIM:606267
Mouse	93735	ENSMUSG00000029671	All phenotypic alleles (3):Targeted, knock-out(1) Targeted, other(2)	MGI:2136018
Xenopus	100125197			484266
Zebrafish	791563	ENSDARG00000094973	ZDB-GENE-040426-2330	ZFIN:ZDB-GENE-040426-2330

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	427856
Ensembl Gene
KEGG	gga:427856

Entry 1

GEISHA Id	WNT16.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	CGAGTGCTCCTGTGACACCAAAC
Reverse cDNA Template Primer	ATTAACCCTCACTAAAGGCAGCCATCGGGTCCATCTG	cDNA Template Length	525
Forward cDNA Template Probe	show GGAAAAGTGGGTCTTCCTGCCCTGATCCCAGGGACCCCAGTTTCTGATCTTCGACGCAGTAATTGGGCGATTTGTTCAC GTACAGCAGGTCTTCTTTCTGGATTGGTATTTTTCTCTGGCTCTTCTCTTTCCTGCGTAACTTTTTTTTCAGCTTGTCT GATACCTGTATGCTGTTTTCATACTTATCCTTTAAAAACCGGCCAATCTTTTCAAAGGAGGACATGGTTTTCCAGCAAG TTTTCACAGCACACGACCCAGAAACACCATGACAACGGCAGTCCACTGACATCAGCTTTGCTACAGCCTGCCTCCCAGC CTCGTTGTTGTGCAGGTTCATCGCCAGCAGCCCGTTGCCACTCTTTCCTGACGCATTCCTGATGGGCGCATCCAGGAAG CTCCTGCTGAAGGCCATCCCATAGTGGATGTCGTCGGAGCAGCCGCCCCAGTGCCAGCCCTCGCTGGCCGAGCCCCCGC CCTGCAGTTTGGTGTCACAGAAGNCACTCGAA
Comments	No expression detected at or before stage 8.

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Unlabeled

Stages 4-6.

7-12

stage 8

stage 9

stage 10

stage 12

Forebrain
Neural Plate/Tube
Pineal Gland
Somites
Unlabeled

Dorsal view.
Low level expression.
Ventral view.

13-18

stage 13

stage 16

Neural Plate/Tube
Pineal Gland
Somites

Dorsal view.
Ventral view.

19-24

stage 21

stage 23

stage 24

Eye
Heart
Leg Mesenchyme
Myotome
Nasal Placode/Nerve
Pharyngeal Arches and Clefts
Pineal Gland
Somites
Spinal Ganglia and Nerves

stage 25

Eye
Leg Mesenchyme
Nasal Placode/Nerve
Pharyngeal Arches and Clefts
Pineal Gland
Spinal Ganglia and Nerves
Wing Mesenchyme

Entry 2

GEISHA Id	Wnt16.Fokina.2006	Data Source	Publication
Complete cDNA Template Probe	show CGAAGGAGTCCGCGTTTGTGTATGCGGTGACGGCAGCAGGGCTGGTGCATGCCGTGACCCGCTCCTGCAGCGCAGGAAA CGTGACCGAGTGCTCCTGTGACACCAAACTGCAGGGCGGGGGCTCGGCCAGCGAGGGCTGGCACTGGGGCGGCTGCTCC GACGACATCCACTATGGGATGGCCTTCAGCAGGAGCTTCCTGGATGCGCCCATCAGGAATGCGTCAGGAAAGAGTGGCA ACGGGCTGCTGGCGATGAACCTGCACAACAACGAGGCTGGGAGGCAGGCTGTAGCAAAGCTGATGTCAGTGGACTGCCG TTGTCATGGTGTTTCTGGGTCGTGTGCTGTGAAAACTTGCTGGAAAACCATGTCCTCCTTTGAAAAGATTGGCCGGTTT TTAAAGGATAAGTATGAAAACAGCATACAGGTATCAGACAAGCTGAAAAAAAAGTTACGCAGGAAAGAGAAGAGCCAGA GAAAAATACCAATCCAGAAAGAAGACCTGCTGTACGTGAACAAATCGCCCAATTACTGCGTCGAAGATCAGAAACTGGG GATCCCTGGGACTCAGGGCAGGGAATGCAACCGCACGTCAGATGGACCCGATGGCTGCAACCTGCTGTGCTGCGGGCGC GGGTACAACACGCACGTGGTCAGGCACGTGGAGAGGTGTGAGTGCAAGTTTGTCTGGTGCTGCTACGTGCGCTGTCGGC GGTGCGAGACCATGACTGACGTACACACCTGCAAGTAGGGCTCAGCACAGAAACAG
Citation	Fokina VM, Frolova EI. Expression patterns of Wnt genes during development of an anterior part of the chicken eye. Dev Dyn. 2006 Feb;235(2):496-505.
Copyright	Copyright © 2008 Wiley-Liss, Inc.

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Eye
Notochord

25-30

stage 26

Eye
Retina

stage 31

Eye

Entry 3

GEISHA Id	WNT16.Geetha-Loganathan.2009	Data Source	Publication
Complete cDNA Template Probe	show CGAAGGAGTCCGCGTTTGTGTATGCGGTGACGGCAGCAGGGCTGGTGCATGCCGTGACCCGCTCCTGCAGCGCAGGAAA CGTGACCGAGTGCTCCTGTGACACCAAACTGCAGGGCGGGGGCTCGGCCAGCGAGGGCTGGCACTGGGGCGGCTGCTCC GACGACATCCACTATGGGATGGCCTTCAGCAGGAGCTTCCTGGATGCGCCCATCAGGAATGCGTCAGGAAAGAGTGGCA ACGGGCTGCTGGCGATGAACCTGCACAACAACGAGGCTGGGAGGCAGGCTGTAGCAAAGCTGATGTCAGTGGACTGCCG TTGTCATGGTGTTTCTGGGTCGTGTGCTGTGAAAACTTGCTGGAAAACCATGTCCTCCTTTGAAAAGATTGGCCGGTTT TTAAAGGATAAGTATGAAAACAGCATACAGGTATCAGACAAGCTGAAAAAAAAGTTACGCAGGAAAGAGAAGAGCCAGA GAAAAATACCAATCCAGAAAGAAGACCTGCTGTACGTGAACAAATCGCCCAATTACTGCGTCGAAGATCAGAAACTGGG GATCCCTGGGACTCAGGGCAGGGAATGCAACCGCACGTCAGATGGACCCGATGGCTGCAACCTGCTGTGCTGCGGGCGC GGGTACAACACGCACGTGGTCAGGCACGTGGAGAGGTGTGAGTGCAAGTTTGTCTGGTGCTGCTACGTGCGCTGTCGGC GGTGCGAGACCATGACTGACGTACACACCTGCAAGTAGGGCTCAGCACAGAAACAG
Citation	Geetha-Loganathan, P, Nimmagadda, S, Antoni, L, Fu, K, Whiting, C, Francis-West, P and Richman, J. Expression of WNT signalling pathway genes during chicken craniofacial development. DEVELOPMENTAL DYNAMICS Volume 238, Issue 5, May 2009, Pages: 11501165.
Copyright	Dev. Dyn Copyright © 2009 John Wiley & Sons, Inc. All Rights Reserved.
Comments	This paper indicated the probe sequence was obtained using the accession number AY753296 for WNT16.

Stage

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Location (click to highlight)

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13-18

stage 18

Pharyngeal Arches and Clefts
Pineal Gland

19-24

stage 20

Pharyngeal Arches and Clefts
Surface Ectoderm

epithelium of the lateral nasal prominence

25-28

stage 28

Pharyngeal Arches and Clefts
Surface Ectoderm

epithelium lining the nasal
slit

Entry 4

GEISHA Id	WNT16.Kee.2007	Data Source	Publication
Complete cDNA Template Probe	show CCGCCCGCCGCCTTCGGGCAGCAGCTGAGCAGCGGCACGAAGGAGTCCGCGTTTGTGTATGCGGTGACGGCAGCAGGGC TGGTGCATGCCGTGACCCGCTCCTGCAGCGCAGGAAACGTGACCGAGTGCTCCTGTGACACCAAACTGCAGGGCGGGGG CTCGGCCAGCGAGGGCTGGCACTGGGGCGGCTGCTCCGACGACATCCACTATGGGATGGCCTTCAGCAGGAGCTTCCTG GATGCGCCCATCAGGAATGCGTCAGGAAAGAGTGGCAACGGGCTGCTGGCGATGAACCTGCACAACAACGAGGCTGGGA GGCAGGCTGTAGCAAAGCTGATGTCAGTGGACTGCCGTTGTCATGGTGTTTCTGGGTCGTGTGCTGTGAAAACTTGCTG GAAAACCATGTCCTCCTTTGAAAAGATTGGCCGGTTTTTAAAGGATAAGTATGAAAACAGCATACAGGTATCAGACAAG CTGAAAAAAAAGTTACGCAGGAAAGAGAAGAGCCAGAGAAAAATACCAATCCAGAAAGAAGACCTGCTGTACGTGAACA AATCGCCCAATTACTGCGTCGAAGATCAGAAACTGGGGATCCCTGGGACTCAGGGCAGGGAATGCAACCGCACGTCAGA TGGACCCGATGGCTGCAACCTGCTGTGCTGCGGGCGCGGGTACAACACGCACGTGGTCAGGCACGTGGAGAGGTGTGAG TGCAAGTTTGTCTGGTGCTGCTACGTGCGCTGTCGGCGGTGCGAGACCATGACTGACGTACACACCTGCAAGTAGGGCT CAGCACAGAAACAG
Citation	Yun Kee, Byung Joon Hwang, Paul W. Sternberg, Marianne Bronner-Fraser. Evolutionary conservation of cell migration genes: from nematode neurons to vertebrate neural crest. Genes Dev. 2007 February 15; 21(4): 391396.
Copyright	Copyright © 2007, Cold Spring Harbor Laboratory Press
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #XM_001233677.2 ).