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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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ERG
ERG, ETS transcription factor

Gene

Official Symbol	ERG
Official Full Name	ERG, ETS transcription factor
CGNC ID	49062
also known as	transcriptional regulator Erg\|v-ets avian erythroblastosis virus E26 oncogene homolog\|v-ets erythroblastosis virus E26 oncogene homolog
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_204280
Polypeptide	NP_989611

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	2078	ENSG00000157554		MIM:165080
Mouse	13876	ENSMUSG00000040732	All phenotypic alleles (2):Chemically induced(1) Targeted, other(1)	MGI:95415
Xenopus	100216056, 378618, 378619			6085046
Zebrafish	494073		ZDB-GENE-041212-40	ZFIN:ZDB-GENE-041212-40

Gene Ontology

Molecular Function	sequence-specific DNA binding, sequence-specific DNA binding transcription factor activity
Biological Process	transcription, DNA-templated
Cellular Component	nucleus, ribonucleoprotein complex

Links to other databases

Entrez Gene	374146
Ensembl Gene	ENSGALG00000016058
KEGG	gga:374146

Entry 1

GEISHA Id	pgm2n.pk011.i24	Data Source	GEISHA ISH Analysis
Forward cDNA Template Probe	show AACCAATGAACGAAGAGTTATTGTGCCAGCAGATCCTACGTTATGGAGCACAGACCATGTACGGCAGTGGCTGGAGTGG GCAGTGAAGGAGTATGGTCTTCCAGACGTGGACATCTTGTTGTTCCAGAACATTGATGGGAAAGAGTTGTGTAAAATGA CCAAAGATGACTTCCAGAGACTCACGCCGAGCTATAACGCAGATATCCTCCTGTCACACCTACACTACCTCAGAGAGAC TCCTCTTCCACATTTGACTTCAGATGATGTTGATAAGGCCTTACAAAACTCTCCACGGTTAATGCATGCTAGAAACACA GGAGGAGCCACTTTTATTTTTCCAAATACATCAGTTTACCCAGAAGCAACGCAAAGAATAACAACAAGGCCAGATTTAC CTTATGAGCAAGCGAGGAGATCAGCGTGGACGAGTCACAGCCATCCCACTCAGTCAAAAGCTACCCAACCATCATCTTC AACAGTGCCCAAAACAGAAGACCAGCGTCCTCAGTTAGATCCTTATCAGATTCTTGGACCGACCAGCAGCCGTCTTGCA AATCCAGGGAGTGGGCAGATACAGCTATGGCAGTTCCTACTGGAGCTTCTGTCGGACAGCTCCAACTCCAACTGCATCA CCTGGGAGGGCACAAATGGGGAGTTCAAGATGACAGACCCTGATGAAGTGGCTCGGCGTTGGGGAGAGAGGAAAAGCAA ACCTAACATGAACTATGACAAACTCAGCCGTGCACTTCGCTACTACTATGACAAAAATATTATGACTAAAGTTCATGGT AAACGCTATGCCTACAAATTTGATTTCCACGGAATCGCTCAGGCCCTCCAGCCTCACCCTCAGAATCATCCATGTACAA ATACCCATCAGACCTCCCCTACATGAGTTTCCTACATGCACACCCCCAAAAGAGAACTTTGTAGCTCCCCATCCCCCTG

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 25

Head Mesenchyme
Heart
Leg Mesenchyme
Pharyngeal Arches and Clefts
Somites
Wing Mesenchyme

Entry 2

GEISHA Id	ERG.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATGCAAAAGGAGGGAAAATGGTTAGC
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGATGAGGTTACGGGCAAAGCAGG	cDNA Template Length	1063
Complete cDNA Template Probe	show TATGGAGCACAGACCATGTACGGCAGTGGCTGGAGTGGGCAGTGAAGGAGTATGGTCTTCCAGACGTGGACATCTTGTT GTTCCAGAACATTGATGGGAAAGAGTTGTGTAAAATGACCAAAGATGACTTCCAGAGACTCACGCCGAGCTATAACGCA GATATCCTCCTGTCACACCTACACTACCTCAGAGAGACTCCTCTTCCACATTTGACTTCAGATGATGTTGATAAGGCCT TACAAAACTCTCCACGGTTAATGCATGCTAGAAACACAGGAGGAGCCACTTTTATTTTTCCAAATACATCAGTTTACCC AGAAGCAACGCAAAGAATAACAACAAGGCCAGATTTACCTTATGAGCAAGCGAGGAGATCAGCGTGGACGAGTCACAGC CATCCCACTCAGTCAAAAGCTACCCAACCATCATCTTCAACAGTGCCCAAAACAGAAGACCAGCGTCCTCAGTTAGATC CTTATCAGATTCTTGGACCGACCAGCAGCCGTCTTGCAAATCCAGGGAGTGGGCAGATACAGCTATGGCAGTTCCTACT GGAGCTTCTGTCGGACAGCTCCAACTCCAACTGCATCACCTGGGAGGGCACAAATGGGGAGTTCAAGATGACAGACCCT GATGAAGTGGCTCGGCGTTGGGGAGAGAGGAAAAGCAAACCTAACATGAACTATGACAAACTCAGCCGTGCACTTCGCT ACTACTATGACAAAAATATTATGACTAAAGTTCATGGTAAACGCTATGCCTACAAATTTGATTTCCACGGAATCGCTCA GGCCCTCCAGCCTCACCCTCCAGAATCATCCATGTACAAATACCCATCAGACCTCCCCTACATGAGTTCCTACCATGCA CACCCCCAGAAGATGAACTTTGTAGCTCCCCATCCCCCTGCTTTGCCCGTAACCTCATCCAGCTTTTTTGCTGCCCCTA ATCCATACTGGAATTCACCAACTGGAGGCATCTACCCCAATACCAGGCTGCCAGCTGCTCATATGCCTTCCCATCTTGG CACCTACTACTAAGTGGGGAAAGAAAGAAA
Comments	No specific expression detected at or below stage 6.

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Unlabeled

Stages 5-6.

7-12

stage 8

stage 9

stage 11

stage 12

Area Opaca
Blood Islands
Blood Vessels
Head Mesenchyme

Dorsal view.
Ventral view.

13-18

stage 13

Blood Islands
Blood Vessels
Head Mesenchyme

Dorsal view.
Ventral view.

19-23

stage 21

stage 23

Allantois
Head Mesenchyme
Leg
Pharyngeal Arches and Clefts
Somites
Wing

Entry 3

GEISHA Id	Erg.Iwamotoa.2000	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGCGAACGAATAATTATTATTAGCAATTATTAGCGATCAATAATCTTGATCACATTATGGCAAGCACTATTAA GGAAGCATTATCAGTGGTGAGTGAAGACCAGTCCTTGTTTGAGTGTGCCTACGGATCGCCCCACCTTGCAAAGACAGAA ATGACAGCCTCCTCTTCCAGTGAATATGGGCAAACATCAAAGATGAGCCCGCGCGTTCCCCAGCAGGACTGGTTATCAC AGCCCCCGGCCAGAGTTACCATTAAGATGGAGTGTAACCCAAACCAGGTTAATGGGTCAAGGAATTCACCTGATGACTG CAGCGTGGCAAAAGGAGGGAAAATGGTTAGCAGTTCAGACAATGTTGGGATGAACTATGGAAGCTACATGGAAGAGAAG CATATTCCGCCTCCAAATATGACAACCAATGAACGAAGAGTTATTGTGCCAGCAGATCCTACGTTATGGAGCACAGACC ATGTACGGCAGTGGCTGGAGTGGGCAGTGAAGGAGTATGGTCTTCCAGACGTGGACATCTTGTTGTTCCAGAACATTGA TGGGAAAGAGTTGTGTAAAATGACCAAAGATGACTTCCAGAGACTCACGCCGAGCTATAACGCAGATATCCTCCTGTCA CACCTACACTACCTCAGAGAGACTCCTCTTCCACATTTGACTTCAGATGATGTTGATAAGGCCTTACAAAACTCTCCAC GGTTAATGCATGCTAGAAACACAGGAGGAGCCACTTTTATTTTTCCAAATACATCAGTTTACCCAGAAGCAACGCAAAG AATAACAACAAGGCCAGATTTACCTTATGAGCAAGCGAGGAGATCAGCGTGGACGAGTCACAGCCATCCCACTCAGTCA AAAGCTACCCAACCATCATCTTCAACAGTGCCCAAAACAGAAGACCAGCGTCCTCAGTTAGATCCTTATCAGATTCTTG GACCGACCAGCAGCCGTCTTGCAAATCCAGGGAGTGGGCAGATACAGCTATGGCAGTTCCTACTGGAGCTTCTGTCGGA CAGCTCCAACTCCAACTGCATCACCTGGGAGGGCACAAATGGGGAGTTCAAGATGACAGACCCTGATGAAGTGGCTCGG CGTTGGGGAGAGAGGAAAAGCAAACCTAACATGAACTATGACAAACTCAGCCGTGCACTTCGCTACTACTATGACAAAA ATATTATGACTAAAGTTCATGGTAAACGCTATGCCTACAAATTTGATTTCCACGGAATCGCTCAGGCCCTCCAGCCTCA CCCTCCAGAATCATCCATGTACAAATACCCATCAGACCTCCCCTACATGAGTTCCTACCATGCACACCCCCAGAAGATG AACTTTGTAGCTCCCCATCCCCCTGCTTTGCCCGTAACCTCATCCAGCTTTTTTGCTGCCCCTAATCCATACTGGAATT CACCAACTGGAGGCATCTACCCCAATACCAGGCTGCCAGCTGCTCATATGCCTTCCCATCTTGGCACCTACTACTAAGT GGGGAAAGAAAGAAA
Citation	Iwamoto M, Higuchi Y, Koyama E, Enomoto-Iwamoto M, Kurisu K, Yeh H, Abrams WR, Rosenbloom J, and Pacifici M. Transcription Factor ERG Variants and Functional Diversification of Chondrocytes during Limb Long Bone Development. The Journal of Cell Biology, Volume 150, Number 1, July 10, 2000 27-40.
Copyright	NO PERMISSION YET
Comments	Authors said: " To prepare a ch-ERGspecific probe, oligomers containing an EcoRI site at 5′ end and HindIII site at 3′ end were used for PCR of the 81-bp region present in ch-ERG. The PCR product was then subcloned into pGEM3Z vector. A ch-ERG/C-1-1 probe was prepared by amplification of nucleotide 429901 region and subcloning into pGEM-T easy vector (Promega)." As insufficient information is found to determine the exact sequence of the probe used, the sequence shown here is from Acc# NM_204280.1.

no images

Entry 4

GEISHA Id	ERG.Singh.2016	Data Source	Publication
Complete cDNA Template Probe	show ACCTTATGAGCAAGCGAGGAGATCAGCGTGGACGAGTCACAGCCATCCCACTCAGTCAAAAGCTACCCAACCATCATCT TCAACAGTGCCCAAAACAGAAGACCAGCGTCCTCAGTTAGATCCTTATCAGATTCTTGGACCGACCAGCAGCCGTCTTG CAAATCCAGGGAGTGGGCAGATACAGCTATGGCAGTTCCTACTGGAGCTTCTGTCGGACAGCTCCAACTCCAACTGCAT CACCTGGGAGGGCACAAATGGGGAGTTCAAGATGACAGACCCTGATGAAGTGGCTCGGCGTTGGGGAGAGAGGAAAAGC AAACCTAACATGAACTATGACAAACTCAGCCGTGCACTTCGCTACTACTATGACAAAAATATTATGACTAAAGTTCATG GTAAACGCTATGCCTACAAATTTGATTTCCACGGAATCGCTCAGGCCCTCCAGCCTCACCCTCCAGAATCATCCATGTA CAAATACCCATCAGACCTCCCCTACATGAGTTCCTACCATGCACACCCCCAGAAGATGAACTTTGTAGCTCCCCATCCC CCTGCTTTGCCCGTAACCTCATCCAGCTTTTTTGCTGCCCCTAATCCATACTGGAATTCACCAACTGGAGGCATCTACC CCAATACCAGGCTG
Citation	Singh PN, Ray A, Azad K, Bandyopadhyay A. A comprehensive mRNA expression analysis of developing chicken articular cartilage. Gene Expr Patterns. 2016 Jan;20(1):22-31.
Copyright	Copyright © 2015 Elsevier B.V. All rights reserved.
Comments	The complete cDNA template sequence was obtained from the information from the publication as described in Singh et al. 2016.