For cOsr2, two probes were designed based on the predicted sequence XM_418353. We amplified the 5′-coding region without the zinc-finger domain and the full-length ORF for use as probes, respectively, using the primers cOsr2-F: ATGGGCAGCAAGGCGCTGC; cOsr2-R1: GGTTGGCGAAGTCGAAGCG; and cOsr2-R2: TCAGAAGTCCTGCCGCGGGGT. Both probes were PCR amplified from 5-day whole chicken embryonic cDNA and cloned into pCRII-TOPO (Invitrogen). Both probes yielded identical results.
)
