The goals of the GEISHA project are to present whole mount in situ hybridization information and corresponding metadata for genes expressed in the chicken embryos between 0.5 and six days of embryogenesis, and to integrate this information with other genomics resources. In situ hybridization expression information is obtained from three sources:
- In house high throughput in situ hybridization screening. cDNAs are obtained from several embryonic cDNA libraries, from the University of Delaware and the BBSRC EST repositories, and from individual investigators.
- Literature curation. Through agreements with most journals, published in situ hybridization images and information is posted on the GEISHA site.
- Unpublished in situ hybridization information: Laboratories generally publish only a small percentage of their in situ hybridization data. High quality images for which probe identity can be documented are welcome additions to GEISHA.
The GEISHA project is funded by NIH P41HD064559 to Parker Antin
Parker Antin: Principal Investigator
Diana Darnell: Database Support
Sean Davey: Programmer and Database Manager
Tania Yatskievych: Laboratory Support
Maricela Pier: Laboratory Support
Terry Sesepasara: Laboratory Support
Sam Simmers: Laboratory Support
Stella Zhang: Laboratory Support
Jacob Klewer: Laboratory Support
Anne Marie Sjursen
Bell, G.W., Yatskievych, T.A., and Antin, P.B. (2004) GEISHA, a high throughput whole mount in situ hybridization screen in chick embryos. Devel. Dynamics 229: 677-687.
Darnell, D.K., Kaur, S., Stanislaw, S., Davey, S., Konieczka, J.H., Yatskievych, T.A., and Antin, P.B. (2007). GEISHA: An In situ hybridization gene expression resource for the chicken embryo. CytogeNet. Genome Res. 117:30-35
To reference a specific entry in the database:
Data for this paper was retrieved from the GEISHA database, University of Arizona, Tucson, AZ 85724; World Wide Web URL: http://geisha.arizona.edu; [date data was retrieved].
Additional References arising from GEISHA in situ hybridization screens:
Darnell, D.K. Kaur, S., Stanislaw, S., Konieczka, J.H., Yatskievych, T.A., and Antin, P.B. MicroRNAs during chicken embryo development.(2006) Devel. Dyn 235:3156-3165.
Ason, B., Darnell, D.K., Wittbrodt, B., Berezikov, E., Kloosterman, W.P., Wittbrodt, J., Antin, P.B., and Plasterk, R.H.A. (2006). Comparative Analysis of miRNA expression. Proc. Natl. Acad. Sci. USA 103:14385-14389.
Darnell, D.D., Stanislaw, S., Kaur, S., and Antin, P.B. (2010) Whole mount in situ hybridization detection of mRNAs using short LNA Containing DNA Oligonucleotide Probes. RNA 16:632-7. PMCID: PMC2822927
Antin, P.B., Pier, M., Sesepasara, T., Yatskievych, T.A., and Darnell, D.K. (2010). Embryonic Expression of the Chicken Krüppel-like (KLF) Transcription Factor Gene Family. Devel. Dyn. 239:1879-87 PMCID: PMC2925473
Using GEISHA Images:
All site content, including but not limited to, images, movies and text, are copyrighted by the University of Arizona or with the individuals or publications from which data was obtained. You are welcome to download images for personal use. However, no materials from the GEISHA site may be reproduced without permission. Some previously copyrighted materials have been used in the creation of this resource, and in such instances the existing copyright is clearly noted.
Obtaining cDNAs for In situ Hybridization Probes:
cDNAs used to generate probes have been obtained from several sources. These includes embryonic cDNA libraries prepared in our labs, those obtained from other researchers as well as from the BBSRC and University of Delaware (U.D) EST repositories. Due to budgetary and personnel limitations, we are unable to supply cDNAs to the scientific community. However, cDNAs corresponding to almost all probes used to generate data are available for purchase from the BBSRC and U.D EST repositories.