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About
GEISHA:
Background: The GEISHA project began in 1998 to investigate using high throughput whole mount in situ hybridization to identify novel, differentially expressed genes in chicken embryos. An initial expression screen of approximately 900 genes demonstrated feasibility of the approach, and also highlighted the need for a centralized repository of in situ hybridization expression data. Funding was eventually obtained for this purpose. Objectives: The goals of the GEISHA project are to obtain whole mount in situ hybridization expression information for all differentially expressed genes in the chicken embryo between HH stages 1-25, to integrate expression data with the chicken genome browsers, and to offer this information through a user-friendly graphical user interface. In situ hybridization images are obtained from three sources: 1. In house high throughput in situ hybridization screening: cDNAs obtained from several embryonic cDNA libraries or from EST repositories are screened for expression using high throughput in situ hybridization approaches. 2. Literature curation: Agreements with journals permit posting of published in situ hybridization images and related information on the GEISHA site. 3. Unpublished in situ hybridization information from other laboratories: laboratories generally publish only a small fraction of their in situ hybridization data. High quality images for which probe identity can be verified are welcome additions to GEISHA. Funding: The GEISHA project is funded by NIH R01HD044767 to Parker Antin. GEISHA Personnel: Parker Antin: Principal Investigator Diana Darnell: Laboratory Supervisor Tania Yatskievych: Laboratory Support Maricela Pier: Laboratory Support Terry Sesepasara: Laboratory Support Anne Marie Sjursen: Laboratory Support Sean Davey: Internal and Public Web Databases, design and implementation Jay Konieczka: Bioinformatic support Citing GEISHA: For a general reference we recommend citing: Bell, G.W., Yatskievych, T.A., and Antin, P.B. (2004). GEISHA, a gallus EST in situ hybridization approach to large scale gene discovery. Dev. Dyn. 229:677-687. To reference a specific entry in the database: Data for this paper was retrieved from the GEISHA database, University of Arizona, Tucson, AZ 85724; World Wide Web URL: http://geisha.arizona.edu; [date data was retrieved]. Additional References arising from GEISHA in situ hybridization screens: Darnell, D.K. Kaur, S., Stanislaw, S., Konieczka, J.H., Yatskievych, T.A., and Antin, P.B. MicroRNAs during chicken embryo development.(2006) Devel. Dyn 235:3156-3165. Ason, B., Darnell, D.K., Wittbrodt, B., Berezikov, E., Kloosterman, W.P., Wittbrodt, J., Antin, P.B., and Plasterk, R.H.A. (2006). Comparative Analysis of miRNA expression. Proc. Natl. Acad. Sci. USA 103:14385-14389. Using GEISHA Images: All site content, including but not limited to, images, movies and text, are copyrighted by the University of Arizona or with the individuals or publications from which data was obtained. You are welcome to download images for personal use. However, no materials from the GEISHA site may be reproduced without permission. Some previously copyrighted materials have been used in the creation of this resource, and in such instances the existing copyright is clearly noted. Obtaining cDNAs for In situ Hybridization Probes: cDNAs used to generate probes have been obtained from several sources. These includes embryonic cDNA libraries prepared in our labs, those obtained from other researchers as well as from the BBSRC and University of Delaware (U.D) EST repositories. Due to budgetary and personnel limitations, we are unable to supply cDNAs to the scientific community. However, cDNAs corresponding to almost all probes used to generate data are available for purchase from the BBSRC and U.D EST repositories. |
