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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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IRX2
iroquois homeobox 2

Gene

Official Symbol	IRX2
Official Full Name	iroquois homeobox 2
CGNC ID	48986
also known as	iroquois-class homeodomain protein IRX-2\|iroquois homeobox protein 2\|iroquois homologue 2
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_001030336
Polypeptide	NP_001025507

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	153572	ENSG00000170561		MIM:606198
Mouse	16372	ENSMUSG00000001504	All phenotypic alleles (3):Targeted, other(3)	MGI:1197526
Xenopus	493475, 378497			480617
Zebrafish	394032	ENSDARG00000001785	ZDB-GENE-040426-1446	ZFIN:ZDB-GENE-040426-1446

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	373975
Ensembl Gene
KEGG	gga:373975

Entry 1

GEISHA Id	IRX2.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATTCCCCTTACGACGCCCATAC
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGTTTGAAGCCACTGCCGCCGAGTTG	cDNA Template Length	518
Complete cDNA Template Probe	show GGGGATGACCGGGGCCATCAGTACCACCGTAGGCAGCCCTGCCTACCCCTCCAGTCAAGACCCCGCGTACAGGAAGAAT GCCACCCGGACGCCACGGCCACGCTGAAGGCCTGGCTGCAGGAGCACGCAAGAACCCCTACCCCACCAAGGGCGAGAAG ATCATGTGGCCATCATCACCAAGTGCCCTCACCCAGGTCTCCACCTGGTTTGCCAATGCCGCCGCCGCCTCAAGAAGGA GAACAAGATGACGTGGGCCCCGCGGAACAAGAGTGAGGACGAGGACGACGACGATGGCGACGGGGCGAGGATAAGAGAG AGCCCTGACAAGATACCGAAGCACGAGACCTGCCGAGGAGAAGGGATCAGCTTGCGTGGACTCGTGACGGACCACTCGT GCTCCCGAGTCGGACAGCGCTGCTACTCCCTCCTCTCTGGGGAGGCATATTTATTACTCACCTTTTTATAGCAATTACA CAATATGGGAATTTAACGCTCTCCAAGCAGGGGATCCTGAGATA

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Early Mesoderm
Lateral Plate Mesoderm
Paraxial Mesoderm
Unlabeled

Low level expression.

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

stage 12

Anterior Intestinal Portal
Anterior/Second Heart Field
Head Mesenchyme
Heart
Hindbrain
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Paraxial Mesoderm
Twins

Cardiogenic mesoderm
Dorsal view.
Sections pending.

13-18

stage 14

stage 17

Head Mesenchyme
Heart Tube
Hindbrain
Lateral Plate Mesoderm
Liver
Midbrain
Neural Plate/Tube

Sections pending.

19-24

stage 22

Head Mesenchyme
Heart
Hindbrain
Lateral Plate Mesoderm
Limb Buds
Midbrain
Paraxial Mesoderm
Pharyngeal Arches and Clefts
Retina

Sections pending.

stage 25

Head Mesenchyme
Heart
Hindbrain
Lateral Plate Mesoderm
Leg
Midbrain
Myocardium
Paraxial Mesoderm
Pharyngeal Arches and Clefts
Retina
Wing
Wing Mesenchyme

Sections pending.

Entry 2

GEISHA Id	IRX2.Anderson.2021	Data Source	Direct Submission
Complete cDNA Template Probe	show ATGTCCTATCCTCAGGGTTACCTCTACCAGCCCCCCGGCTCGCTGGCTCTGTACTCCTGCCCGGCGTACGGAGCGTCGG CGCTGGCGGCCCCCAGGAGCGAGGAGCTGGCCAGGTCTTCGTCGGGCTCGGCGTTCAGCCCTTATCCGGGATCGGCAGC TTTCACCGCCCAGGCGGCGGCCACAGGCTTCACCAGCCCGCTCCAGTACTCCACAGACCCCGCCACGGGATTCCCCTCC TACATGGGCTCCCCTTACGACGCCCATACGACGGGGATGACCGGGGCCATCAGCTACCACCCGTACGGCAGCCCTGCCT ACCCCTACCAGCTCAACGACCCCGCGTACAGGAAGAATGCCACCCGCGACGCCACGGCCACGCTGAAGGCCTGGCTGCA GGAGCACCGCAAGAACCCCTACCCCACCAAGGGCGAGAAGATCATGCTGGCCATCATCACCAAGATGACCCTCACCCAG GTCTCCACCTGGTTTGCCAACGCCCGCCGCCGCCTCAAGAAGGAGAACAAGATGACGTGGGCCCCGCGAAACAAGAGTG AGGACGAGGACGACGACGATGGCGACGGGGCGAGGAGTAAAGAGGAGAGCCCTGACAAGATACCCGAGAGCAACGAGAC CTCCGCCGAGGACGAAGGGATCAGCTTGCAAGTGGACTCGCTGACGGACCACTCGTGCTCCGCCGAGTCGGACGGCGAG AAGCTGCCGTGCCGGGCCGGCGACCCCCTGTGCGAGTCGGGTTCGGAGTGCAAGGACAAGTACGAGGACATCGAAGAGG AAGAGGAAGACGAGGACGATGACGAGGAGGACATCGAGGAGGACGACGGCGGCGGCGGGGAGCGCGACCCGCCGGCCAA GCCAGCCACCTCCTCGCCTCTGGCCGCCGTGGAGGCCCCTCTGCTCGGCCACCCGCACGCCGACGCCGCCCGCAGCGCC AGCAAGGCGGCGCTGGGCGGCCGCGCCTCCCCCGGGCCCCCGACGCCGGCCAGCAAGCCCAAGCTCTGGTCGCTGGCCG AAATCGCCACCTCGGACCTCAAGAGCCAGAACTTGGGCCAAGGCTGCCCGCCGGCAGCGTTGGCCTCGGCCACCCCCGC CTCCGCCCCGCACAGCGCTGCCTACTCTCCCTCCTCCCTTCTGGGGAGGCATATTTATTACACCTCACCTTTTTATAGC AATTACACAAACTATGGGAACTTTAACGCTCTCCAGAGCCAGGGGATCCTGAGATACAACTCGGCGGCAGTGGCTTCAA ACGAGGGACTCAGTCAGACTGTCCTAAATGCCAGCTCTGTCCACAAGCAGAGCAGTGACTCTTTGAAAACGATCACTAA CCAGCTAGAACAACATTACAGGCCCTCTAGTTATGACTCTAAGAAAGATCCCACTGAAGTCTGCACAGTAGGAGTACAA CCATACCTATAG
Comments	Images provided by Claire Anderson. As insufficient information was provided to verify sequence used to synthesize probe, the sequence above was obtained from NCBI (accession number NM_001030336.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Area Opaca
Lateral Plate Mesoderm
Paraxial Mesoderm

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

stage 12

Amnion
Anterior Intestinal Portal
Atria
Ear/Otic Placode
Ectoderm
Endoderm
Forebrain
Foregut
Head Mesenchyme
Head Process
Heart
Hindbrain
Intermediate Mesoderm
Lateral Plate Mesoderm
Mesencephalon
Midbrain
Myocardium
Neural Crest
Neural Plate/Tube
Paraxial Mesoderm
Pharynx
Sinus Venosus
Somatic Mesoderm
Somites
Splanchnic Mesoderm
Ventricles
Widespread Expression
extraembryonic ectoderm
extraembryonic endoderm
extraembryonic mesoderm

HH12+
HH8-
HH9+
Ventral view
section 1
section 26
section 34
section 56
section 66
section 72

stage 13

Widespread Expression

Entry 3

GEISHA Id	IRX2.Trevers.2023	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show GGGAGAGAGGGAGAGCACGAAAGCAGCGCCGGGAGCAGCGGCAGCAGCAGGGCCGGTCACCCTCGCCCCCTCCCTGCTC CTGTGCTCGCCAGCGCAAGCCATGTCCTATCCTCAGGGTTACCTCTACCAGCCCCCCGGCTCGCTGGCTCTGTACTCCT GCCCGGCGTACGGAGCGTCGGCGCTGGCGGCCCCCAGGAGCGAGGAGCTGGCCAGGTCTTCGTCGGGCTCGGCGTTCAG CCCTTATCCGGGATCGGCAGCTTTCACCGCCCAGGCGGCGGCCACAGGCTTCACCAGCCCGCTCCAGTACTCCACAGAC CCCGCCACGGGATTCCCCTCCTACATGGGCTCCCCTTACGACGCCCATACGACGGGGATGACCGGGGCCATCAGCTACC ACCCGTACGGCAGCCCTGCCTACCCCTACCAGCTCAACGACCCCGCGTACAGGAAGAATGCCACCCGCGACGCCACGGC CACGCTGAAGGCCTGGCTGCAGGAGCACCGCAAGAACCCCTACCCCACCAAGGGCGAGAAGATCATGCTGGCCATCATC ACCAAGATGACCCTCACCCAGGTCTCCACCTGGTTTGCCAACGCCCGCCGCCGCCTCAAGAAGGAGAACAAGATGACGT GGGCCCCGCGGAACAAGAGTGAGGACGAGGACGACGACGATGGCGACGGGGCGAGGAGTAAAGAGGAGAGCCCTGACAA GATACCCGAGAGCAACGAGACCTCCGCCGAGGACGAAGGGATCAGCTTGCAAGTGGACTCGCTGACGGACCACTCGTGC TCCGCCGAGTCGGACGGCGAGAAGCTGCCGTGCCGGGCCGGCGACCCCCTGTGCGAGTCGGGTTCGGAGTGCAAGGACA AGTACGAGGACATCGAAGAGGAAGAGGAAGACGAGGACGATGACGAGGAGGACATCGAGGAGGACGACGGCGGCGGCGG GGAGCGCGACCCGCCGGCCAAGCCAGCCACCTCCTCGCCTCTGGCCGCCGTGGAGGCCCCTCTGCTCGGCCACCCGCAC GCCGACGCCGCCCGCAGCGCCAGCAAGGCGGCGCTGGGCGGCCGCGCCTCCCCCGGGCCCCCGACGCCGGCCAGCAAGC CCAAGCTCTGGTCGCTGGCCGAAATCGCCACCTCGGACCTCAAGAGCCAGAACTTGGGCCAAGGCTGCCCGCCGGCAGC GTTGGCCTCGGCCACCCCCGCCTCCGCCCCGCACAGCGCTGCCTACTCTCCCTCCTCCCTTCTGGGGAGGCATATTTAT TACACCTCACCTTTTTATAGCAATTACACAAACTATGGGAACTTTAACGCTCTCCAGAGCCAGGGGATCCTGAGATACA ACTCGGCGGCAGTGGCTTCAAACGAGGGACTCAGTCAGACTGTCCTAAATGCCAGCTCTGTCCACAAGCAGAGCAGTGA CTCTTTGAAAACGATCACTAACCAGCTAGAACAACATTACAGGCCCTCTAGTTATGACTCTAAGAAAGATCCCACTGAA GTCTGCACAGTAGGAGTACAACCATACCTATAGAAGTACAATCACGCAGCAATGCAAGTAAGTGAGAGCGTTTTCTATT ACGCTGATCATTTGGCACCAGGTCTAAGACAGCGCGCAGATCGAGACTTGCTTAGTGTTAATTTCTTGCTTCATTACAT GAGGCTCTGATTACAGAGTAAGACACTCTTTTCTGCTATTTTTAAGCCTCTCATTACAGCTTTTCTTTCTCGTGTTTGT CTTCATTTTAAAGTCAGGTTAGACTTCCTTCACCTCGCGTACTCTTTGCTTGTGTGAGGCAAACCTGAAAGGACTATTT AAATGCTAAGGAAAAGCAAGCTTGAAAGAATATTTTTGAAGGCGTTGTTCAGAAAAGTTCTCGTGCTCACGTGGCAGTG CTGCAATGGCACATTTTAAGCGAGGTTTCGCACGGCGATCGTGTATTTGAAATGAGAAGGGACGATTATCAGAGATCCA CATGCATTTCAGCAGGGTGTGTTGTCTGTGACTTGCTCCTTGCCCGGCTATCAGTTCAGTGCTTAATACCCCCCATTAC CTGGGGGGGCTAAAGAAACAAAAAAAAATCACCTTGTCGATACTTTTCCTTTTCTTTTTTCTTTCTTTCTTTTATTTAT TTTATTTTATTTTTTGTTAGGAGAAAAAAAAGTAGACGAGTAAGAGTTTTGCCTTACTTCCAGTATTTTTCGAGTTTAA TGGCGTTAGTGTGCAATTGAAGGATTTTTGGATGATCTGGTTTTTTCCTTGGTCCACAGGTAGGTGTCACAATTGCTTT GAAAAAAGTCAGCAAGCCATCATCTCTCCCTGAGCTAATATATATAACAAATCTCTAAATCCGGATCACATCTTGTGCC ACTGTATAAAAGAAGACCACAGAGCACTATTAAATCTTCAGACTCTAATTATTAAACCAGAATTTTGTCGGACATAATG TGAGTTTGCTGGTATAGTATAGTTTCCCCAATGTACGTGTGACTTTTGAAAACAGATCTGTTTTTCTCAGGATATCTCG AATTGATCACTTCATTGCCACGGTATATATTCGATAGGTGTGATAGGATTTATTGTAAAATTTATTTGTAAAAGATGTA TACAGTAGAGATAATAAAGACGTGATTGGAGAACTTGAGTCCTTACAAAGTGGAAACAAATTTGATATTTATTTTTGTA ACGATATAAAGCTTCTAGTAATTTATGCAAGTTGTATTGCAATGGAATCTGAACTTTTTGTAAATAAATTTTGCCTGGT TTTTATTTGAAACGTTGATGGTTATACAATCTTTGGTTGTTTAACGAGAATTCTTTACTAATTTATATCTCTAATTGTA AATAAAAACAGACTAGTCTGCAACAA
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001030336 ).