GEISHA 2.0

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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
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Gene Expression Report

Probe 1

GEISHA Id I38

NCBI Ensembl

Gene Name FLRT3 FLRT3

Aliases FLRT3, fibronectin leucine rich transmembrane protein 3, Official

Gene Description fibronectin leucine rich transmembrane protein 3 Leucine-rich repeat transmembrane protein FLRT3 precursor (Fibronectin-like domain-containing leucine-rich transmembrane protein 3). [Source:Uniprot/SWISSPROT;Acc:Q9NZU0]

Gene Ontology

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NCBI Nucleotide Hit

XM_426107 show details

Ensembl Genome Map contig view

Complete Sequence

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Stage

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1-3

stage 3

Unlabeled

4-6

stage 4

stage 6

7-12

stage 11

stage 12

Anterior Intestinal Portal

13-18

stage 14

Ear/Otic Placcode

Somites

Surface Ectoderm

19-22

stage 22

Probe 2

GEISHA Id	Flrt3.Smith.2006
	NCBI	Ensembl
Gene Name		FLRT3
Gene Description		Leucine-rich repeat transmembrane protein FLRT3 precursor (Fibronectin-like domain-containing leucine-rich transmembrane protein 3). [Source:Uniprot/SWISSPROT;Acc:Q9NZU0]
Ensembl Genome Map	contig view
Citation	Smith TG, Tickle C. The expression of Flrt3 during chick limb development. Int J Dev Biol. 2006;50(8):701-4.
Copyright	"Reprinted from Int. J. Dev. Biol., 50, Smith TG, Tickle C, The expression of Flrt3 during chick limb development, 701-4, Copyright (2006), with permission from Copyright © 2006 UBC Press". All rights reserved.
Comments	According to authors , "The chick Flrt3 orthologue was discovered from a search inthe ENSEMBL Gallus gallus genome browser: Identification ENSGALG00000008716. PCR was performed on stage HH16 whole-embryo cDNA to generate two probes that hybridized to different regions of the cFlrt3 gene. Primers used were 1) For: 5’- AGATGAATTCCCCACTAACCTC -3’ and Rev: 5’- ACGTGCGGCCGCATCAGCCCACGTACGTTCAC -3’. 2) For: 5’- ACGTGAATTCATGACATGAGCACCATCCAG -3’ Rev: 5’- ACGTGCGGCCGCGGAATACCACTGTCTCTGTAG -3’. Each PCR product was cloned into pBluescript (KS)." As information provided was insufficient to verify exact probe used for photos, the two probes are shown as 5' and 3' reads drawn from NCBI (acc # XM_426107).
5' Sequence	show AGATGAATTCCCCACTAACCTCCCTAAGTACGTCAAAGAACTGCATTTGCAGGAGAATAATATAAGGACCATCACTTAC GATTCACTTTCACAAATTCCTTATCTGGAAGAACTGCATTTGGATGATAATTCTGTTTCCGCTGTTAGCATTGAAGATG GAGCTTTCAGGGACAACATCTATCTCAGACTTCTTTTTCTCTCTCGAAATCACCTTAGCACCATTCCCTGGGGTTTGCC TAAAACAATAGAAGAGCTACGCTTGGATGATAATCGTATTTCCACGATTTCAGAGCTGTCCCTTCAAGACCTTACAAAT CTAAAACGCCTTGTTCTAGATGGAAATCTTCTAAATAACCACGGATTAGGAGACAAGGTCTTTATGAATCTAGTCAATC TTACAGAACTGTCATTGGTTCGCAATTCACTTACAGCCGCACCAGTAAATTTGCCAGGAACAAACCTAAGGAAGCTTTA TCTGCAAGAAAACCATATCAATCACGTGCCACCCAATGCTTTCTCTTACTTAAGGCAGTTGTATCGACTAGATATGTCC AATAACAATCTCAGCAATTTACCTCAGGGTGTCTTTGATGACCTGGACAACATAACTCAGCTGTTTCTTCGCAACAACC CTTGGCACTGTGGGTGCAAAATGAAGTGGGTCCGTGACTGGTTACAGTCATTGCCTTTGAAAGTGAACGTACGTGGGCT GATG
3' Sequence	show ATGACATGAGCACCATCCAGATCACTACTGCGGTACCCAACACGCTGTACCCGGCCCAGGGACACTGGCCGGTTTCTGT GACCAAACAACCAGACATCAAGACCCCCAACCTAAACAAGAACTACAGAACCACGGCCAGCCCAGTACGCAAGATCATT ACAATATTTGTGAAATCGGTGAGCACAGAGACCATCCACATCTCCTGGAAAGTTGCGCTGCCAATGACAGCCTTACGGC TCAGCTGGCTCAAGATGGGCCACAGCCCTGCCTTTGGATCTATAACTGAAACCATCGTAACAGGGGACAGGAGTGACTA CCTGCTTACAGCACTCGAACCAGAGTCGCCATACCGCGTGTGCATGGTCCCCATGGAAACCAGCAACATCTATCTCTCT GACGAGACACCTGAATGCATCGAGACCGAGACAGCTCCCCTCAAGATGTACAACCCGACAACCACACTCAACCGGGAGC AGGAAAAGGAGCCCTACAAGAACTCCAGCGTGCCTCTGGCCGCCATCATCGGTGGCGCGGTGGCACTGGTGGCCCTGGC ACTGCTGGCCCTGGTGTGCTGGTACGTCCACAGGAATGGGGCCCTCTTCTCCCGGCACTGCGCCTACAGCAAGGGGCGC AGGAGGAAGGACGACTATGCTGAGGCGGGTACCAAGAAGGACAACTCCATCCTAGAAATCAGGGAGACTTCTTTTCAGA TGATACCCATCACTAACGACCAGGTGTCCAAGGAGGAGTTTGTAATACACACCATTTTCCCCCCTAATGGCATGAATCT GTATAAGAACAGCCACAGTGAAAGCAGTAGTAACAGGAGCTACAGAGACAGTGGTATTCC