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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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ACTA1
actin, alpha 1, skeletal muscle

The human orthologue of this gene is associated
with the following human diseases:

Gene

Official Symbol	ACTA1
Official Full Name	actin, alpha 1, skeletal muscle
CGNC ID	8420
also known as	actin, alpha skeletal muscle\|a-actin\|alpha-actin-1, Alpha-actin-1, Actin, alpha skeletal muscle
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_001031063
Polypeptide	NP_001026234

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	58	ENSG00000143632	MIM:102610	MIM:102610
Mouse	11459	ENSMUSG00000031972	All phenotypic alleles (1):Targeted, knock-out(1)	MGI:87902
Xenopus	448346, 432314, 394698, 379752			491937, 480346
Zebrafish	407658, 550445	ENSDARG00000055618, ENSDARG00000036371	ZDB-GENE-030131-55, ZDB-GENE-050417-267	ZFIN:ZDB-GENE-030131-55, ZFIN:ZDB-GENE-050417-267

Gene Ontology

Molecular Function	frizzled binding
Biological Process	Wnt signaling pathway, camera-type eye photoreceptor cell differentiation, more...
Cellular Component	proteinaceous extracellular matrix

Links to other databases

Entrez Gene	421534
Ensembl Gene	ENSGALG00000011086
KEGG	gga:421534

Entry 1

GEISHA Id	ACTA1.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	GAACACGGCATCATCACAAACTG
Reverse cDNA Template Primer	ATTAACCCTCACTAAAGGATCTGCTGGAAGGTGGACAGGGAG	cDNA Template Length	851
Forward cDNA Template Probe	show AAGAAGGAACCACCGAATCCAGNACAGAGTACTTGCGCTCAGGGTGGGGCAATGATCTTGATCTTCATTGGTGCTGGGG GCCAGGGCTGTGATCTCCTTTTGCATGCGGTCAGCAATACCTGGGTACATGGTGGTACCCCCAGACATGACGTTGTTGG CATACAGGTCCTTCCTGATGTCAATGTCGCATTTCATGATGCTGTTGTAAGTTGTCTCATGGATCCCAGCGGACTCCAT ACCAATGAAGGAAGGCTGGAANAGGGTTTCTGGGCANCGGAAACGCTCATTGCCGATGGTGATGACCTGCCCATCAGGC AGCTCATAGCTCTTCTCCAGGGAGGAGGAGGAGGCAGCGGTGGCCATCTCGTTCTCAAAGTCCAGAGCCACATAGCACA GCTTCTCCTTGATGTCACGGACAATCTCACGTTCAGCTGTGGTGACAAAGGAATAGCCACGCTCAGTCAGGATCTTCAT CAGGTAGTCAGTGAGGTCGCGGCCAGCCAGATCCAAGCGCATGATGGCATGGGGCAGGGCATACCCTTCATAGATGGGG ACGTTGTGTGTCACACCATCGCCGGAGTCCAGCACAATACCGGTGGTACGGCCAGAGGCATACAGGGACAGCACAGCCT GGATGGCCACGTACATGGCGGGCACNTTAAAGGTCTCAAACATGATTTGGGTCATCTTTTCACGGTTAGCTTTGGGATT AAGGGGGGCCTCANTGANCAGGGTGGGGTGCTCCTCAGGGGCCACACGCANCTCATTGTAAAAGGTGTGGTGCCAAATC TTCTCCATGTCGTCCCAGTTTGTGATGAGCCCNTGTTCAA

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Area Opaca
Early Endoderm
Early Mesoderm
Ectoderm
Endoderm
Extraembryonic Mesoderm
Lateral Plate Mesoderm
Neural Plate/Tube
Primitive Streak

7-12

stage 8

stage 9

stage 11

Amnion
Blood Vessels
Ectoderm
Endoderm
Extraembryonic
Extraembryonic Blood Vessels
Extraembryonic Mesoderm
Forebrain
Heart
Hindbrain
Intermediate Mesoderm
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Notochord
Primitive Streak
Somites
Ubiquitous

13-18

stage 14

stage 17

stage 18

Ectoderm
Endoderm
Extraembryonic Blood Vessels
Heart
Lateral Plate Mesoderm
Sclerotome
Somites
Spinal Cord
Ubiquitous

Entry 2

GEISHA Id	ACTA1.Bertola.2008	Data Source	Publication
Complete cDNA Template Probe	show ATGTGTGACGAGGACGAGACCACCGCGCTCGTGTGCGACAACGGCTCCGGCCTAGTGAAGGCTGGCTTCGCCGGGGATG ACGCCCCCAGGGCCGTGTTCCCTTCCATCGTGGGCCGGCCCCGCCACCAAGGTGTCATGGTGGGTATGGGTCAGAAGGA CTCCTACGTAGGAGATGAAGCTCAGAGCAAGAGAGGTATCCTCACCCTGAAGTACCCCATTGAACACGGCATCATCACA AACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAATGAGCTGCGTGTGGCCCCTGAGGAGCACCCCACCC TGCTCACTGAGGCCCCCCTTAATCCCAAAGCTAACCGTGAAAAGATGACCCAAATCATGTTTGAGACCTTTAACGTGCC CGCCATGTACGTGGCCATCCAAGCTGTGCTGTCCCTGTATGCCTCTGGCCGTACCACCGGTATTGTGCTGGACTCCGGC GATGGTGTGACACACAACGTCCCCATCTATGAAGGGTATGCCCTGCCCCATGCCATCATGCGCTTGGATCTGGCTGGCC GCGACCTCACTGACTACCTGATGAAGATCCTGACTGAGCGTGGCTATTCCTTTGTCACCACAGCTGAACGTGAGATTGT CCGTGACATCAAGGAGAAGCTGTGCTATGTGGCTCTGGACTTTGAGAACGAGATGGCCACCGCTGCCTCCTCCTCCTCC TTGGAGAAGAGCTATGAGCTGCCTGATGGGCAGGTCATCACCATCGGCAATGAGCGTTTCCGCTGCCCAGAAACCCTCT TCCAGCCTTCCTTCATTGGTATGGAGTCCGCTGGGATCCATGAGACAACTTACAACAGCATCATGAAATGCGACATTGA CATCAGGAAGGACCTGTATGCCAACAACGTCATGTCTGGGGGTACCACCATGTACCCAGGTATTGCTGACCGCATGCAA AAGGAGATCACAGCCCTGGCCCCCAGCACAATGAAGATCAAGATCATTGCCCCACCTGAGCGCAAGTACTCTGTCTGGA TCGGTGGCTCCATCTTGGCCTCCCTGTCCACCTTCCAGCAGATGTGGATCACAAAGCAGGAGTATGATGAAGCCGGCCC ATCCATTGTCCACCGTAAATGCTTCTAA
Citation	Bertola LD, Ott EB, Griepsma S, Vonk FJ, Bagowski Developmental expression of the alpha-skeletal actin gene. CP.BMC Evol Biol. 2008 Jun 2;8:166.
Copyright	© 2008 Bertola et al; licensee BioMed Central Ltd.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001031063).

Stage

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Location (click to highlight)

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19-24

stage 20

Heart
Lens
Limb Buds
Mesencephalon
Metencephalon
Pharyngeal Arches and Clefts
Somites

stage 25

Ear/Otic Placode
Heart
Limb Buds
Pharyngeal Arches and Clefts
Somites

Entry 3

GEISHA Id	ACTAskel.Ruzicka.1988	Data Source	Publication
Complete cDNA Template Probe	show ATGTGTGACGAGGACGAGACCACCGCGCTCGTGTGCGACAACGGCTCCGGCCTAGTGAAGGCTGGCTTCGCCGGGGATG ACGCCCCCAGGGCCGTGTTCCCTTCCATCGTGGGCCGGCCCCGCCACCAAGGTGTCATGGTGGGTATGGGTCAGAAGGA CTCCTACGTAGGAGATGAAGCTCAGAGCAAGAGAGGTATCCTCACCCTGAAGTACCCCATTGAACACGGCATCATCACA AACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAATGAGCTGCGTGTGGCCCCTGAGGAGCACCCCACCC TGCTCACTGAGGCCCCCCTTAATCCCAAAGCTAACCGTGAAAAGATGACCCAAATCATGTTTGAGACCTTTAACGTGCC CGCCATGTACGTGGCCATCCAAGCTGTGCTGTCCCTGTATGCCTCTGGCCGTACCACCGGTATTGTGCTGGACTCCGGC GATGGTGTGACACACAACGTCCCCATCTATGAAGGGTATGCCCTGCCCCATGCCATCATGCGCTTGGATCTGGCTGGCC GCGACCTCACTGACTACCTGATGAAGATCCTGACTGAGCGTGGCTATTCCTTTGTCACCACAGCTGAACGTGAGATTGT CCGTGACATCAAGGAGAAGCTGTGCTATGTGGCTCTGGACTTTGAGAACGAGATGGCCACCGCTGCCTCCTCCTCCTCC TTGGAGAAGAGCTATGAGCTGCCTGATGGGCAGGTCATCACCATCGGCAATGAGCGTTTCCGCTGCCCAGAAACCCTCT TCCAGCCTTCCTTCATTGGTATGGAGTCCGCTGGGATCCATGAGACAACTTACAACAGCATCATGAAATGCGACATTGA CATCAGGAAGGACCTGTATGCCAACAACGTCATGTCTGGGGGTACCACCATGTACCCAGGTATTGCTGACCGCATGCAA AAGGAGATCACAGCCCTGGCCCCCAGCACAATGAAGATCAAGATCATTGCCCCACCTGAGCGCAAGTACTCTGTCTGGA TCGGTGGCTCCATCTTGGCCTCCCTGTCCACCTTCCAGCAGATGTGGATCACAAAGCAGGAGTATGATGAAGCCGGCCC ATCCATTGTCCACCGTAAATGCTTCTAA
Citation	Ruzicka DL, Schwartz RJ. Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation. J Cell Biol. 1988 Dec;107(6 Pt 2):2575-86.
Copyright	© The Rockefeller University Press,
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001031063.1).