Authors state: Whole-mount and section in situ hybridizations using digoxigenin- and fluorescein-labeled antisense RNA probes were performed as previously described ( Adams et al., 2000). Because insufficient information provided to verify exact probe sequence, sequence was obtained from NCBI (Acc# AB278131).
TCATACCAGCACCTAACAGTGCCTTCTCTATCACGGGCGCTGCTGCCAGCATGAAGGACAAGCCATGCAGCCCCACCAG CGGGTCACCCACAGCTGGTACAGCAGCCAGTTCAGAACACATAATGCAACCTAAACCTACCTCAGTGGTGTTGGCTGCC ACCGGCGGTGAAGAAGCCATGAATCTCATTAAAAGCAAGAGGAATGTGACTGGTTACAAAACCCTCCCATACCCACTGA AGA
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The probe was amplified by PCR using the following primers:
5_-TCATACCAGCACCTAACAGTGCCT-3_ and 5_-TCTTCAGTGGGTATGGGAGGGTTT-3_ and was extracted from NCBI Acc# XM_003641038.1
Zwane TB, Nikitina NV. Spatiotemporal expression analysis of Prdm1 and Prdm1 binding partners in early chick embryo. Gene Expr Patterns. 2015 Jan;17(1):56-68. Epub 2015 Feb 17. DOI: 10.1016/j.gep.2014.12.003.