Geisha

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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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LBH
limb bud and heart development

Gene

Official Symbol	LBH
Official Full Name	limb bud and heart development
CGNC ID	51464
also known as	protein LBH\|limb bud and heart development homolog, limb bud and heart development homolog
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_001031038
Polypeptide	NP_001026209
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.1108

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	81606	ENSG00000213626		MIM:611763
Mouse	77889	ENSMUSG00000024063	All phenotypic alleles (3):Targeted, other(3)	MGI:1925139
Xenopus	496858, 397879			5945475
Zebrafish

Gene Ontology

Molecular Function
Biological Process	regulation of transcription, DNA-templated, transcription, DNA-templated
Cellular Component	cytoplasm, nucleus

Links to other databases

Entrez Gene	421301
Ensembl Gene	ENSGALG00000009052
KEGG	gga:421301

Entry 1

GEISHA Id	ChEST353o13	Data Source	GEISHA ISH Analysis
Forward cDNA Template Probe	show GCTCCTGCAGGCACCTGCAGAAGGGGATCGCTGTCAGACTGGAAGTAATACAAAGACTCAACACCGGACAGTGTGGATT ATCAGTGAGGAGGTGGAGGGGAGCTCTCTGCGGAGTTGTTAGCCTGAGCATCCTGAGACAAGGCATCTGTTTTGTGGCA AGGATGAGATAGCTCTTCATGGGTGTTTAATGGAGCACAGACTGTTCTGTGAAGTCTGTTTGGGTCCTTCTCACCACAC CATATCCTGCTCCAGTGGGGAGTGGTCTGTCTCTGTCTGGGGAGGAGGGCAGTCTGCTGCCATGTGGGGTAACTCACCA TTTTCCTGGAAGGTGATGCTCTTTGTGGCTTTGTGGGCCAGCTCAGGGAGCCAGTTGCTCTTGGAATAGGATGAGAACA GTTACTACCTAAACCAAATCCGGGGTCACAGAAGAGTAAAAGAAATGTCAAGGCCTTAACCAAACTTTCATAATAATCC CAATGCCACCCTCCTTTGCTTGTAACAGGTTCCATTTTTAAAATCTATTTGAACATGTGTATTTGTATTTTTAATACCT CATGAGTAGTTGAACGCTGGCAGGGGTCGGGAACTGCTTTTAACATATTAGCAGGATCATTTTAGTCTTGTCTTGCTTG TGTTTTAAACATAATGGCAATAACTTTTCAGCTCTACATAGCTGTTTTCAGCAAATAGGAGTTTGGAGATGAGCTTGTG CAAAGCAGGAGAGA

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 11

stage 12

Atria
Heart Tube
Outflow Tract
Ventricles

13-18

stage 13

stage 14

stage 15

Atria
Extraembryonic
Heart Tube
Outflow Tract
Ventricles

19-24

stage 21

stage 24

Lateral Plate Mesoderm
Outflow Tract
Ventricles

Entry 2

GEISHA Id	LBH.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATGGGTCTGTAGCAAAGCATCACG
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGCACAAAGAGCATCACCTTCCAGG	cDNA Template Length	927
Complete cDNA Template Probe	show TTTTTTAAAAAACCTGCGGCCGCAGAATATGGTCTGTAGCAAAGCATCCGAAATGCTCTAACGCCTGGGCCTGTAGCTT TCCCGTTCAGCACCTCCTAGGGTCCAGTGAGCATCCTAGGAGTAGTGGGCTTCTTGCTCTCGCTCTTGAGCCTGTACCA GTCACTTTCCAAAAGCTGTCTTTTCACCCTCTGTTTGCCTTTAGACCTTTCAATGCTGAACAGCTGTATTTGCAGGTAA TGTTAGGCTGCTGCTTGTCATCTCTACTGCTGCAAGGCCCTAGTTGATCTTGCAGGCGGTTAGCTTGAGGTAGTACAAA TTCGATGTAGAGACATCTGTTTGCAGCATGAACTAATGTAGATTTGAACACGTGGATTTTTCTCTCCTATTTGCCAAGG CTACTGTGGTTGGTCTCGTCTTGTTCCGGTGCCCAAAGCTGAAGGGTGAGGATTGCACCTGCTTGCCTCGATGTCTTTG AGGCTGTAGGGTTTCATATCTCAACTTGCTGGTCAGTGGGCTTGCGAGGGTTGGAGACGGGCTTGGCTGCTCCTGCAGG CACCTGCAGAAGGGGATCGCTGTCAGACTGGAAGTAATACAAAGACTCAACACCGGACAGTGTGGATTATCAGTGAGGA GGTGGAGGGGAGCTCTTGCGGAGTTGTTAGCCTGAGCATCCTGAGACAAGGCATCTGTTTTGTGGCAAGGATGAGATAG CTCTTCATGGGTGTTTAATGGAGCACAGACTGTTCTGTGAAGTCTGTGTTTGGGTCCTTCTCACCACACCATATCCTGC TCCAGTGGGAAGACGGTCTGTCTCTGTTTGGGGAGGAGGGCAGTCCTGCTGCCCACGTGGGGTAACCTCACCTTTGGAA GGATGCTCTTTGTGCCTTATGAGGGTATTTTAAAAAAAA

Stage

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Location (click to highlight)

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1-3

stage 3

Hensen's Node

4-6

stage 4

stage 5

stage 6

Head Process
Hensen's Node

Sections pending.

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

stage 12

Anterior Intestinal Portal
Blood Islands
Forebrain
Head Mesenchyme
Head Process
Heart Tube
Paraxial Mesoderm

HH 8-
Sections pending.

13-18

stage 17

Ear/Otic Placode
Eye
Head Mesenchyme
Heart
Lateral Plate Mesoderm
Somites

sections pending.

19-24

stage 20

stage 21

stage 24

Allantois
Endoderm
Head Mesenchyme
Heart
Lateral Plate Mesoderm
Lens

Sections pending.

Entry 3

GEISHA Id	LBH.Conen.2009	Data Source	Publication
Complete cDNA Template Probe	show GACTTCATGTCTGTGCTTTGTCCCCTGCCCTGCCCTGATTACCTGAGATCAGCCGAGATGACCGAAGTGATGATGAGCA CCCCGGCTATGGATGAGATCGGACTCAGCCCCCGTAAAGATGGGCTCTCGTATCAGATTTTCCCTGATCCCTCTAACTT TGACTGTTACTGCAAGCTGAAGGACCGCCTGCCCTCCATCGTGGTGGAGCCCACGGAGGGTGATGTGGAGAGCGGGGAG CTGAGATGGCCGCCTGAGGAGTTCCTCGTCCAGGAGGAGGAAGAGGAGGAAAGCTGTGAAGATGCAAAGGAGGACAACA AGGAGCAATAAATGGCATCTGAGGAAAGGCAAGGGACTGCACCCTTCTGAAGGAAGAGCCACACTTTTTTTGAAAGTCT TTTTAAAGAGAAAAGACAAGTTCAACTTTGCACCTGCAAGATTTTAGTTTTTTCGTATTCTCTGTATTGAGAACTGAAG CCCTTGGTGTCACCAGACCTTCTGAGGAAGGAAGGAAGTAACGCAGAGAAATGTTTGCTGTTCTCGAGATCACTATCTG TATTCTTCTGAGGTGGAGGCAGCAGTGATGTTCCAAGCACGTGGCGGAGGGTGACCACCTGAGGGATGCTGGAGGGGGG AACGTGGGGATGGGGAGTGCAGAGGGAAGCCAAGCAGAGCAGAGTTCAGCTGGGAGCTGCCTCTGACCAGAAGCCTGAG AACAGAGCAGTCATCTCAGATTTGCTTTTCCTAAAGCTTCCATTTTCTGTGTCTGGAGGTGGAAGGGTCTGTAGCAAAG CATCACGAAATGCTCTAACGCTGAGGGACCTGTAGCTTTCCCCAGTTCAGCACCTCCTAGGGTCCAGTGAGCATCCTTA GGAGTAGTGGGCTTCTTGCTCTCGCTCTTGAGCCTGTACCAGTCACTTTCCAAAAGCTGTCTTTTCACCCTCTGTTTGC CTTTAGACCTTTCAATGCTGAACAGCTGTATTTGCAGGGTAATGTTAGGCTGCTGCTTGTCATCTCTACTGCTGCAAGG CCCTAGTTGATCTTGCAGGCGGTTAGCTTGAGGTAGTACAAATTTGATGTAGAGACATCTGTTTGCAGCATGAACTAAT GTAGATTTGAACACGTGGATTTTTCTCTCCTATTTGCCAAGGCTACTGTGGTTGGTCTCGTCTTGTTCCGGTGCCCAAA GCTGAAGGGTGAGGATTGCACCTGCTTGCCTCTATGTCTTTGAGGCTGTAGGGTTTCATATCTCAACTTGCTGGTCAGT GGGCTTGCGAGGGTTGGAGACGGGCTTGGCTGCTCCTGCAGGCACCTGCAGAAGGGGATCGCGGTCAGACTGGAAGTAA TACAAAGACTCAACACCGGACAGTGTGGATTATCAGTGAGGAGGTGGAGGGGAGCTCTCTGCGGAGTGTTAGCCTGAGC ATCCTGAGACAAGGCATCTGTTTTGTGGCAAGGATGAGATAGCTCTTCATGGGTGTTTAATGGAGCACAGACTGTTCTG TGAAGTCTGTGTTTGGGTCCTTCTCACCACACCATATCCTGCTCCAGTGGGGAGCGGTCTGTCTCTGTTTGGGGAGGAG GGCAGTCTGCTGCCATGTGGGGTAACTCACCATTTTCCTGGAAGGTGATGCTCTTTGTGGCTTTGTGGGCCAGCTCAGG GAGCCAGTTGCTCTTGGAATAGGATGAGAACAGTTACTACCTAAACCAAATCCGGGGTCACAGAAGAGTAAAAGAAATG TCAAGGCCTTAACCAAACTTTCATAATAATCCCAATGCCACCCTCCTTTGCTTGTAACAGGTTCCATTTTTAAAATCTA TTTGAACATGT
Citation	Conen KL, Nishimori S, Provot S, Kronenberg HM. The transcriptional cofactor Lbh regulates angiogenesis and endochondral bone formation during fetal bone development. Dev Biol. 2009 Sep 15;333(2):348-58. Epub 2009 Jul 14.
Copyright	Copyright © 2009 Elsevier B.V. All rights reserved.
Comments	F- 5'CATGTCTGTGCTTTG R- 5' ACATGT

Stage

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Location (click to highlight)

Comments (click to highlight)

31-39

stage 39

Wing
Wing Bones

Entry 4

GEISHA Id	LBH.Alev.2010	Data Source	Publication
Complete cDNA Template Probe	show CTCTAACGCTGAGGGACCTGTAGCTTTCCCCAGTTCAGCACCTCCTAGGGTCCAGTGAGCATCCTTAGGAGTAGTGGGC TTCTTGCTCTCGCTCTTGAGCCTGTACCAGTCACTTTCCAAAAGCTGTCTTTTCACCCTCTGTTTGCCTTTAGACCTTT CAATGCTGAACAGCTGTATTTGCAGGGTAATGTTAGGCTGCTGCTTGTCATCTCTACTGCTGCAAGGCCCTAGTTGATC TTGCAGGCGGTTAGCTTGAGGTAGTACAAATTTGATGTAGAGACATCTGTTTGCAGCATGAACTAATGTAGATTTGAAC ACGTGGATTTTTCTCTCCTATTTGCCAAGGCTACTGTGGTTGGTCTCGTCTTGTTCCGGTGCCCAAAGCTGAAGGGTGA GGATTGCACCTGCTTGCCTCTATGTCTTTGAGGCTGTAGGGTTTCATATCTCAACTTGCTGGTCAGTGGGCTTGCGAGG GTTGGAGACGGGCTTGGCTGCTCCTGCAGGCACCTGCAGAAGGGGATCGCGGTCAGACTGGAAGTAATACAAAGACTCA ACACCGGACAGTGTGGATTATCAGTGAGGAGGTGGAGGGGAGCTCTCTGCGGAGTGTTAGCCTGAGCATCCTGAGACAA GGCATCTGTTTTGTGGCAAGGATGAGATAGCTCTTCATGGGTGTTTAATGGAGCACAGACTGTTCTGTGAAGTCTGTGT TTGGGTCCTTCTCACCACACCATATCCTGCTCCAGTGGGGAGCGGTCTGTCTCTGTTTGGGGAGGAGGGCAGTCTGCTG CCATGTGGGGTAACTCACCATTTTCCTGGAAGGTGATGCTCTTTGTGGCTTTG
Citation	Alev C, Wu Y, Kasukawa T, Jakt LM, Ueda HR, Sheng G. Transcriptomic landscape of the primitive streak. Development. 2010 Sep 1;137(17):2863-74. Epub 2010 Jul 28.
Copyright	Published by The Company of Biologists 2010. This material is protected by a copyright retained by the authors. Permission granted by Dr. G Sheng.
Comments	The exact sequence used to synthesize probe was obtained using RT-PCR primers Forward- CTCTAACGCTGAGGGACCTG Reverse- CAAAGCCACAAAGAGCATCA