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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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DLX5
distal-less homeobox 5

Gene

Official Symbol	DLX5
Official Full Name	distal-less homeobox 5
CGNC ID	48983
also known as	homeobox protein DLX-5\|cDlx\|distal-less homeo box 5, Homeobox protein DLX-5 , cDlx, Distal-less 5
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_204159
Polypeptide	NP_989490

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	1749	ENSG00000105880		MIM:600028
Mouse	13395	ENSMUSG00000029755		MGI:101926
Xenopus	447997, 399265			853056
Zebrafish	30569	ENSDARG00000042296	ZDB-GENE-990415-49	ZFIN:ZDB-GENE-990415-49

Gene Ontology

Molecular Function	HMG box domain binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding, more...
Biological Process	BMP signaling pathway, anatomical structure formation involved in morphogenesis, axon guidance, more...
Cellular Component	cytoplasm, nuclear chromatin

Links to other databases

Entrez Gene	373969
Ensembl Gene
KEGG	gga:373969

Entry 1

GEISHA Id	Dlx5.Bhattacharyya.2004	Data Source	Publication
Complete cDNA Template Probe	show GGGCGCCGAGCCGCCTCCCCCGGCCATGTCCGCCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGG TCCCCGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCC CACTTTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCT ACCTCGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCG CCAAAGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGAGCCTACGGCCGCGGGCAAAGCTCCGC GGGGCAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGG ACTATTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGG CCGAGCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAA GATCATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCG CCGGCGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGT CCCCCGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGG CTCCCTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGTGCTTCTTTTGTATTCCGC TCCCTGGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAA CAAACAAACGACGACGACAACGACAAACAAACAAACAAACAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTT ATTGCATTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTC AAATCTATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAAT TAAACTGTATATTCTGAATAAAATAAAATTATTTCGACC
Citation	Bhattacharyya S, Bailey AP, Bronner-Fraser M, Streit A. Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression. Dev Biol. 2004 Jul 15;271(2):403-14.
Copyright	Reprinted from Developmental Biology, Volume 271, Issue 2, Sujata Bhattacharyya, Andrew P. Bailey, Marianne Bronner-Fraser, and Andrea Streit, Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression, Pages 403-414, Copyright 2004, with permission from Elsevier. Copyright © 2004 Elsevier B.V. All rights reserved.
Comments	Authors indicated the probe came from cDNAs for Dlx-5 Ferrari et al., 1995 who said:Dlx-5 is Acc# U25274.

Stage

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Location (click to highlight)

Comments (click to highlight)

7-10

stage 7

stage 10

Lens
Nasal Placcode/Nerve
Neural Plate/Tube
Surface Ectoderm

Entry 2

GEISHA Id	Dlx5.Zhu.2006	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Zhu H, Bendall AJ. Dlx3 is expressed in the ventral forebrain of chicken embryos: implications for the evolution of the Dlx gene family. Int J Dev Biol. 2006;50(1):71-5.
Copyright	"Reprinted from Int. J. Dev. Biol. Copyright © 2007 UBC Press". All rights reserved.
Comments	According to authors , "Antisense chicken Dlx5 riboprobe was synthesized with T7 RNA polymerase from a pBluescriptSK-Dlx5 template linearized with BamHI (ref)" As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204159).

Stage

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Location (click to highlight)

Comments (click to highlight)

25-29

stage 27

stage 28

stage 29

Diencephalon
Heart
Pharyngeal Arches and Clefts
Telencephalon

Hypothalamus
Sub-ventricular zone
Ventral thalamus

Entry 3

GEISHA Id	Dlx5.Bendall.2003	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Bendall AJ, Hu G, Levi G, Abate-Shen C. Dlx5 regulates chondrocyte differentiation at multiple stages. Int J Dev Biol. 2003 Jun;47(5):335-44.
Copyright	"Reprinted from Int. J. Dev. Biol., 47, Bendall AJ, Hu G, Levi G, Dlx5 regulates chondrocyte differentiation at multiple stages, 335-44, Copyright (2003), with permission from Copyright © 2003 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_204159).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 23

Unlabeled

25-30

stage 26

Wing
Wing Bones

31-44

stage 32

stage 38

stage 44

Wing
Wing Bones

Comparison with other genes
Perichondrium

Entry 4

GEISHA Id	Dlx5.Holleville.2007	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Holleville N, Matéos S, Bontoux M, Bollerot K, Monsoro-Burq AH. Dlx5 drives Runx2 expression and osteogenic differentiation in developing cranial suture mesenchyme. Dev Biol. 2007 Apr 15;304(2):860-74.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204159.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-30

stage 30

Face Mesenchyme

31-38

stage 38

Face Mesenchyme

Entry 5

GEISHA Id	Dlx5.Bendall.2004	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Comments	Insufficient information given in the paper to determine the probe. This sequence taken from NCBI Acc.# NM_204159.1.

no images

Entry 6

GEISHA Id	DLX5.Streit.2002	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Streit, A. Extensive cell movements accompany formation of the otic placode. 2002 DEVELOPMENTAL BIOLOGY 249(2), 237-54.
Copyright	"Reprinted from Developmental Biology, 249(2), Streit A., Extensive cell movements accompany formation of the otic placode, 237-54, Copyright (2002), with permission from Elsevier". Copyright © (2002) Elsevier B.V. All rights reserved.
Comments	According to the authors, "cDNAs ... were kind gifts (see txt for refs)." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence was obtained from NCBI (acc# NM_204159).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

stage 6

Epiblast
Headfold
Neural Plate/Tube
Surface Ectoderm

double labeled

7-12

stage 7

stage 8

Headfold
Lateral Plate Mesoderm
Neural Crest
Surface Ectoderm

double label

31-44

stage 44

Entry 7

GEISHA Id	DLX5.McLarren.2003	Data Source	Publication
Complete cDNA Template Probe	show `GCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGGCGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGG CCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCCCGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCC GCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCCCTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCT ACTGAGGGCCGAA`
Citation	McLarren KW, Litsiou A, Streit A. DLX5 positions the neural crest and preplacode region at the border of the neural plate. Dev Biol. 2003 Jul 1;259(1):34-47.
Copyright	Copyright © 2003 Elsevier Science (USA). All rights reserved.
Comments	The sequence below was obtained from NCBI (NM_204159) using the information provided in the publication.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-8

stage 8

Ectoderm
Neural Plate/Tube

Neural folds

Entry 8

GEISHA Id	DLX5.Holleville.2003	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Holleville N, Quilhac A, Bontoux M, Monsoro-Burq AH. BMP signals regulate Dlx5 during early avian skull development. Dev Biol. 2003 May 1;257(1):177-89.
Copyright	Copyright © 2003 Elsevier Science (USA) All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204159).

Stage

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Location (click to highlight)

Comments (click to highlight)

31-42

stage 35

stage 38

stage 42

Head Mesenchyme

Bone Blastemas
Skull

Entry 9

GEISHA Id	DLX5.Riley.2003	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Riley BB, Phillips BT. Ringing in the new ear: resolution of cell interactions in otic development. Dev Biol. 2003 Sep 15;261(2):289-312. Review.
Copyright	Copyright © 2003 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204159).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Early Embryo
Ectoderm
Headfold

Medial Preplacodal Domain

Entry 10

GEISHA Id	DLX5.Khudyakov.2009	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Khudyakov J, Bronner-Fraser M. Comprehensive spatiotemporal analysis of early chick neural crest network genes. Dev Dyn. 2009 Mar;238(3):716-23.
Copyright	Published Online: 22 Feb 2009 Copyright © 2009 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204159).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Ectoderm
Placcode

Neural plate border
Non-neural ectoderm
Preplacodal region

7-10

stage 7

stage 8

stage 9

stage 10

Ectoderm
Endoderm
Nasal Placcode/Nerve

Non-neural ectoderm

Entry 11

GEISHA Id	DLX5.Bhattacharyya.2008	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204159).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 8

stage 11

Ear/Otic Placcode
Ectoderm
Nasal Placcode/Nerve
Neural Crest
Neural Plate/Tube

Anterior ectoderm
Anterior neural folds
Dorsal neural tube
Emigrating neural crest
Olfactory precursors

13-18

stage 15

Ectoderm
Nasal Placcode/Nerve
Neural Crest

Olfactory placodes

19-20

stage 19

stage 20

Cranial Ganglion
Ear/Otic Placcode
Nasal Placcode/Nerve
Pharyngeal Arches and Clefts

Entry 12

GEISHA Id	DLX5.Bailey.2006	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Bailey AP, Bhattacharyya S, Bronner-Fraser M, Streit A. Lens specification is the ground state of all sensory placodes, from which FGF promotes olfactory identity. Dev Cell. 2006 Oct;11(4):505-17.
Copyright	Copyright © 2006, Elsevier
Comments	Insufficient information provided in publication to verify exact sequence to synthesize probe. Sequence below was obtained from NCBI (NM_204159).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Area Opaca
Early Embryo

7-12

stage 10

Ectoderm
Nasal Placcode/Nerve

presumptive olfactory ectoderm

19-20

stage 20

Entry 13

GEISHA Id	DLX5.Basch.2006	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Basch ML, Bronner-Fraser M, García-Castro MI. Specification of the neural crest occurs during gastrulation and requires Pax7. Nature. 2006 May 11;441(7090):218-22.
Copyright	Copyright © 2006, Nature Publishing Group

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-5

stage 4

stage 5

Placode

double labeled

Entry 14

GEISHA Id	DLX5.Paxton.2010	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Paxton CN, Bleyl SB, Chapman SC, Schoenwolf GC. Identification of differentially expressed genes in early inner ear development. Gene Expr Patterns. 2010 Jan;10(1):31-43. Epub 2009 Nov 11.
Copyright	2009 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204159).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

Early Mesoderm
Epiblast
Neural Plate/Tube
Primitive Streak

7-11

stage 7

stage 8

stage 11

Anterior Intestinal Portal
Ear/Otic Placode
Ectoderm
Endoderm
Head Mesenchyme
Mesoderm
Neural Plate/Tube
Primitive Streak
Tail

Entry 15

GEISHA Id	DLX5.Gibson.2011	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Gibson A, Robinson N, Streit A, Sheng G, Stern CD. Regulation of programmed cell death during neural induction in the chick embryo.Int J Dev Biol. 2011;55(1):33-43.
Copyright	"Reprinted from Int. J. Dev. Biol., 55(1), Copyright (2011), with permission from the authors and IJDB, Copyright © 2002 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204159).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Epiblast
Germinal Crescent

STAGE 4+

Entry 16

GEISHA Id	DLX5.SzaboRogers.2009	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Szabo-Rogers HL, Geetha-Loganathan P, Whiting CJ, Nimmagadda S, Fu K, Richman JM. Novel skeletogenic patterning roles for the olfactory pit. Development. 2009 Jan;136(2):219-29. Epub 2008 Dec 4.
Copyright	Published by The Company of Biologists 2009. This material is protected by a copyright retained by the authors. Permission requested from Dr. Richman.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204159.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 15

Ear/Otic Placode
Face Mesenchyme
Nasal Placode/Nerve
Pharyngeal Arches and Clefts

C' and right side show normal expression

19-20

stage 20

Nasal Placode/Nerve
Pharyngeal Arches and Clefts

D' and right side show normal expression

Entry 17

GEISHA Id	DLX5.Acloque.2011	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Hervé Acloque, Oscar H. Ocaña, Ander Matheu, Karine Rizzoti, Clare Wise, Robin Lovell-Badge, M. Angela Nieto Reciprocal Repression between Sox3 and Snail Transcription Factors Defines Embryonic Territories at Gastrulation. Dev Cell. 2011 September 13; 21(3): 546558.
Copyright	Copyright © 2011 Published by Elsevier Inc.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204159.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Epiblast

Entry 18

GEISHA Id	DLX5.Sanchez-Arrones.2012	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Sanchez-Arrones L, Stern CD, Bovolenta P, Puelles L. Sharpening of the anterior neural border in the chick by rostral endoderm signaling. Development. 2012 Mar;139(5):1034-44.
Copyright	Published by The Company of Biologists © 2012.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_204995.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Epiblast

DLX5(purple)
SIX3(brown)
control embryo
double-labeled in situ:

Entry 19

GEISHA Id	DLX5.Bardet.2008	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Bardet SM, Martinez-de-la-Torre M, Northcutt RG, Rubenstein JL, Puelles L. Conserved pattern of OTP-positive cells in the paraventricular nucleus and other hypothalamic sites of tetrapods. Brain Res Bull. 2008 Mar 18;75(2-4):231-5. Epub 2007 Nov 20.
Copyright	Copyright © 2012 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204159.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

31-34

stage 31

stage 34

Forebrain

Entry 20

GEISHA Id	DLX5.Ferrari.2006	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Ferrari D, Kosher RA. Expression of Dlx5 and Dlx6 during specification of the elbow joint. Int J Dev Biol. 2006;50(8):709-13.
Copyright	© UBC Press
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_204159.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 23

stage 24

Wing

25-26

stage 25

stage 26

Wing

Entry 21

GEISHA Id	cDLX5.Adamska.2001	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Adamska M, Herbrand H, Adamski M, Krüger M, Braun T, Bober E. FGFs control the patterning of the inner ear but are not able to induce the full ear program. Mech Dev. 2001 Dec;109(2):303-13.
Copyright	Copyright © 2001 Elsevier Science Ireland Ltd.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence was obtained from NCBI (NM_204159.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 15

Ear/Otic Placode
Pharyngeal Arches and Clefts

31-44

stage 44

Entry 22

GEISHA Id	Dlx‐5.Ferrari.1999	Data Source	Publication
Complete cDNA Template Probe	show CCATGGCCTGCAACTCCCCGCAGTCGCCGGCGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCA CGGGCACCCGCCCGCCGCCAACCCGTCCCCCGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCC CTCGGCTCCCACCTGCAGCCCCACGGCTCCCTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGA ACCCTCGTGCTTCTTTTGTATTCCGCTCCCTGGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGA TGGGGGATTTTTAAAGGGAAACGAAACAAACAAACGACGACGACAACGACAAACAAACAAACAAACAAAAAAGGTTAAA ATGTGTAAAGCTTGTGCATGTAACTTATTGCATTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAG CTGTGGACACTGTCAATGGTGCCTTCAAATCTATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCC TTGTAAATAATTGTAGATAGAGGAATTAAACTGTATATTCTGAATAAAATAAAATTATTTCGACC
Citation	Ferrari D, Harrington A, Dealy CN, Kosher RA. Dlx-5 in limb initiation in the chick embryo. Dev Dyn. 1999 Sep;216(1):10-5.
Copyright	Copyright © 1999 WILEY-LISS, INC.
Comments	The complete cDNA template sequence was obtained from the information in the publication as described in Ferrari et al. 1999.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

Intermediate Mesoderm
Lateral Plate Mesoderm
Leg Ectoderm
Somites
Surface Ectoderm
Wing Ectoderm

flank ectoderm

13-18

stage 14

stage 15

stage 16

stage 18

Leg Ectoderm
Limb Buds
Wing AER
Wing Ectoderm

19-21

stage 21

Wing AER
Wing Ectoderm

all

Entry 23

GEISHA Id	DLX5.Anderson.2020	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Comments	Images provided by Claire Anderson. As insufficient information was provided to verify sequence used to synthesize probe, the sequence was obtained from NCBI (accession number: NM_204159). Related paper: Nat Commun. 2016 7:12656. doi: 10.1038/ncomms12656. A strategy to discover new organizers identifies a putative heart organizer. Anderson C, et al. PMID:27557800.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XII

Unlabeled

stage EGK XII+

1-3

stage 3

Epiblast
Primitive Streak

4-6

stage 4

stage 5

stage 6

Early Mesoderm
Ectoderm
Epiblast
Headfold
Lateral Plate Mesoderm
Neural Plate/Tube
Primitive Streak

stage 4+

7-12

stage 7

stage 8

stage 9

stage 11

stage 12

Anterior Neuropore
Ear/Otic Placode
Ectoderm
Eye
Forebrain
Headfold
Lateral Plate Mesoderm
Nasal Placode/Nerve
Neural Crest
Neural Plate/Tube
Notochord
Primitive Streak
Tail
extraembryonic ectoderm

8b
Section 4
olfactory placode precursor
stage 12+
stage 8+
stage 8-

13-18

stage 14

Ear/Otic Placode
Ectoderm
Head Mesenchyme
Nasal Placode/Nerve
Neural Crest
Neural Plate/Tube
Notochord
Pharyngeal Arches and Clefts
Tail

stage 14+

19-24

stage 21

stage 24

Ear/Otic Placode
Head Mesenchyme
Leg Mesenchyme
Nasal Placode/Nerve
Pharyngeal Arches and Clefts
Tail
Wing Mesenchyme

Entry 24

GEISHA Id	DLX5.Freter.2012	Data Source	Publication
Complete cDNA Template Probe	show CGCCGACCGCCTCCCCCGGCCATGTCCGNCTGAGCCCGGCGATCCGCGCTATGACAGCAGTGTTTGACAGAAGGGTCCC CGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCACCCGTCCCAGGAATCTCCCACT TTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCGCACGGCTACTGCTCGCCTACCT CGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCTCCGCCGGCACCTACCCCGCCAA AGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGGCCGCGGGCAAAGCTCCGCGGGG CAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAGAAAGTGCGCAAACCGCGGACTA TTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACCTCGCCCTGCCCGAGCGGGCCGA GCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAAAAGGTCCAAGATCAAGAAGATC ATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCCTGCAACTCCCCGCAGTCGCCGG CGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACCCGCCCGCCGCCAACCCGTCCCC CGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTCCCACCTGCAGCCCCACGGCTCC CTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGNGCTTCTTTGTATTCCGCTCCCT GGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGATTTTTAAAGGGAAACGAAACAAAC AAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAGGTTAAAATGTGTAAAGCTTGTGCATGTAACTTATTGCA TTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTCGGCTCAGCTGTGGACACTGTCAATGGTGCCTTCAAATCT ATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAATAATTGTAGATAGAGGAATTAAACT GTATTTCTGAATAAAATAAAATTATTTCGNCCNAAAAAAAAAAAAAAAAAA
Citation	Freter S, Muta Y, O'Neill P, Vassilev VS, Kuraku S, Ladher RK. Pax2 modulates proliferation during specification of the otic and epibranchial placodes. Dev Dyn. 2012 Nov;241(11):1716-28
Copyright	Copyright © 2012 Wiley Periodicals, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence was obtained from NCBI (NM_204159.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-14

stage 14

Cranial Nerve
Ear/Otic Placode

control = left side of embryo
control = left side of embryo
cross section of figure 4J;
neuroblast
stage 13/14, 16 to 22 somites;

all

Entry 25

GEISHA Id	DLX5.Brown.2005	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show AGCAGTCTGCCGCAGCCGCCGCGCCGAGCCGCCTCCCCCGGCCATGTCCGCCTGAGCCCGGCGATCCGCGCTATGACAG CAGTGTTTGACAGAAGGGTCCCCGGCATCAGGTCCTCCGACTTCCAGCCGCCTTTCCAGAGCGCCGCAGCCATGCACCA CCCGTCCCAGGAATCTCCCACTTTACCCGAGTCCTCGGCTACGGATTCCGACTACTACAGCCCGACGGGGGCGGCCCCG CACGGCTACTGCTCGCCTACCTCGGCTTCCTACGGCAAAGCGCTCAACCCCTACCAGTACCAGTACGGCATGAACGGCT CCGCCGGCACCTACCCCGCCAAAGCCTACGCGGACTACGGCTACGGCAGCCCCTACCACCAGTACGGAGGCGCCTACGG CCGCGGGCAAAGCTCCGCGGGGCAGCCAGAGAAGGAGGTGGCGGAGCCCGAGGTGAGGATGGTGAACGGCAAGCCAAAG AAAGTGCGCAAACCGCGGACTATTTATTCCAGCTTTCAGCTGGCGGCGTTGCAGAGGAGGTTCCAGAAGACCCAATACC TCGCCCTGCCCGAGCGGGCCGAGCTGGCCGCCTCGCTGGGACTCACGCAGACGCAGGTGAAAATCTGGTTCCAGAACAA AAGGTCCAAGATCAAGAAGATCATGAAGAACGGGGAGATGCCCCCGGAGCACAGCCCCAGCTCCAGCGACCCCATGGCC TGCAACTCCCCGCAGTCGCCGGCGGTGTGGGAACCTCAGGGCTCGTCGCGCTCCCTCGGCCACCACGGGCACGGGCACC CGCCCGCCGCCAACCCGTCCCCCGGCAGCTACCTGGAGAGCCCCTCGGCCTGGTACCCCGCCGCCAGCCCCCTCGGCTC CCACCTGCAGCCCCACGGCTCCCTACAGCATCCCTTGGCGCTGCCCTCCGGGACGATCTACTGAGGGCCGAACCCTCGT GCTTCTTTTGTATTCCGCTCCCTGGACTCCTGTGTTTTACTGTTAAGGAAGAATAACGAAAGGAATGCGGATGGGGGAT TTTTAAAGGGAAACGAAACAAACAAACGACGACGACAACGACAAACAAACAAACAAAAAAAAAAGGTTAAAATGTGTAA AGCTTGTGCATGTAACTTATTGCATTTCAAAGGAACCCTTTTTTATACCGTGCGGACGTTTTTTCGGCTCAGCTGTGGA CACTGTCAATGGTGCCTTCAAATCTATGACCTCAACTTTTCCAGAGACTTTTTTTCAATGTTATTTTAACCCTTGTAAA TAATTGTAGATAGAGGAATTAAACTGTATATTCTGAATAAAATAAAATTATTTCG
Citation	Stephen T Brown 1, Juemei Wang, Andrew K Groves. Dlx gene expression during chick inner ear development. J Comp Neurol. 2005 Feb 28;483(1):48-65.0
Copyright	© 2005 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence was obtained from NCBI (NM_204159.2).