GEISHA - A Chicken Embryo Gene Expression Database
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EFNA2
ephrin A2

Gene
Official SymbolEFNA2
Official Full Nameephrin A2
CGNC ID49502
also known asELF-1, ephrin-A2|EPH-related receptor tyrosine kinase ligand 6|Eph ligand family-1|LERK-6
gene typeprotein-coding
Genomic Map
 Ensembl ID not known
NCBI
UCSC
Gallus-Browse
Sequence Information
Genomic
RNANM_204983
PolypeptideNP_990314
Sequence Clusters/ESTsUnigene
Gene Expression
In Situ HybridizationGEISHA
EST ProfileGga.2416
Orthology
Entrez GeneEnsembl GeneGenetic PhenotypesMOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish
Gene Ontology
Molecular Function
Biological Processephrin receptor signaling pathway
Cellular Componentanchored component of membrane, plasma membrane
Links to other databases
Entrez Gene395831
Ensembl Gene
KEGGgga:395831

Entry 1

GEISHA IdEFNA2.UApcrData SourceGEISHA ISH Analysis
Forward cDNA Template PrimerTTATAAAAGCTTGCGGCCGCAGAATATACCGCTACGCCGTCTATTGG
Reverse cDNA Template PrimerGCTCTAGAAATTAACCCTCACTAAAGGAGTTGTTGCTGGTGAAGATGGGcDNA Template Length444
Forward cDNA Template Probeshow
AACGCAGCAACCCCAGGGTTCACCGGNGGGGGATTACACCGNTGGAGGTGAGCATCAATGACTACCTGGGACATCTACT
GCCCTCACTACGAGGAGCCGCTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGC
GTCCTGCGACCACCGGCAGAAGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTC
TCAGAGAAGTTCCAGCTCTTCACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTG
CGTCTCCCCCGAACGTGGTGGACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGA
GTCCCCAGAGCCCATCTTCACCAGCAACAACTCCTTTAGTGAGGGTTAATTTCTAGAGCAAA
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 5
stage 6
Early Mesoderm
Extraembryonic Mesoderm
Headfold
Lateral Plate Mesoderm
Neural Plate/Tube
 
7-12
stage 7
stage 9
stage 10
stage 11
stage 12
Head Mesenchyme
Headfold
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Somites
 
13-18
stage 17
stage 18
Head Mesenchyme
Lateral Plate Mesoderm
Midbrain
Wing
 
19-24
stage 20
stage 21
Head Mesenchyme
Limb Buds
Midbrain
Pharyngeal Arches and Clefts
 
25
stage 25
Head Mesenchyme
Leg Mesenchyme
Pharyngeal Arches and Clefts
Wing Mesenchyme
 

Entry 2

GEISHA IdEphrinA2.Baker.2003Data SourcePublication
Complete cDNA Template Probeshow
CCGCGCTGGGAGGCGGCCGCGCTGCTCGCCGCGATCGTCGGCGTCTGCGTGTGGAGCGACGACCCCGGCAAAGTGATCT
CGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAGGTTCCACCGCGGGGATTACACCGTGGAGGTGAGCATCAA
TGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCGCTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATG
GTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGAAGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCC
CCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTTCACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCA
CGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTGGACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCG
ACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCACCAGCAACAACTCGTGCTGCAGCCTTGCAGTGCCGCGTG
CCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGGCTCTTAG
CitationBaker RK, Antin PB. Ephs and ephrins during early stages of chick embryogenesis Dev Dyn. 2003 Sep;228(1):128-42.
Copyright2003 Wiley-Liss, Inc.
CommentsEphrinA2 transcripts are present throughout the mesoderm (as determined from sectioned embryos, not shown) during stages 4-7; during this period, low levels of transcripts are also present in the primitive streak (Fig. 1A-C). Between stages 7 and 12, ephrinA2 is strongly expressed throughout the neurogenic ectoderm, particularly in the posterior half of the mesencephalon (the prospective midbrain). After stage 7, ephrinA2 expression continues in the head mesenchyme and cardiogenic mesoderm but is down-regulated in the mesoderm posterior to the heart-forming region.
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 4
Area Pellucida
Epiblast
Mesoderm, early
area pellucida
early mesoderm
7-10
stage 7
stage 10
Heart
Mesoderm, early
Midbrain
Neural Plate/Tube
Primitive Streak
cardiogenic mesoderm
head mesenchyme
rostral mesoderm

Entry 3

GEISHA IdELF-1.Dube.2007Data SourcePublication
Complete cDNA Template Probeshow
CCGCCGCCCGCTGAGCCGCTCCGCTCCGCCGGGACGATGCCGCGCTGGGAGGCGGCCGCGCTGCTCGCCGCGATCGTCG
GCGTCTGCGTGTGGAGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAG
GTTCCACCGCGGGGATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCG
CTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGA
AGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTT
CACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTG
GACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCA
CCAGCAACAACTCGTGCTGCAGCCTTGCAGTGCCGCGTGCCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGG
CTCTTAGTGCCGCGGGACGGACGGACGGGACGG
CitationDube A, Thai S, Gaspar J, Rudders S, Libermann TA, Iruela-Arispe L, Oettgen P. Elf-1 is a transcriptional regulator of the Tie2 gene during vascular development. Circ Res. 2001 Feb 2;88(2):237-44.
CopyrightCopyright © 2007 UBC Press
CommentsAuthors said The cELF-1 cDNA fragment used to generate the probe is 700 bp in length and encodes the first 100 amino acids of the cELF-1 protein and 100 bp of the 5' untranslated region. The sequence listed above is NCBI Acc# NM_204983.
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
31-36
stage 36
stage 36
Blood Vessels
Heart Tube
Yolk Sack or Stalk
 

Entry 4

GEISHA IdEPHRINA2.Takahashi.2003Data SourcePublication
Complete cDNA Template Probeshow
AGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAGGTTCCACCGCGGGG
ATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCGCTGCCCGCCGAGCG
CATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGAAGGGCTTCAAACGT
TGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTTCACCCCCTTCTCTT
TGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTGGACAGACCCTGCCT
CAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCACCAGCAACAACTCG
TGCTGCAGCCTTGCAGTGCCGCGTGCCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGGCT
CitationTakahashi H, Shintani T, Sakuta H, Noda M. CBF1 controls the retinotectal topographical map along the anteroposterior axis through multiple mechanisms. Development. 2003 Nov;130(21):5203-15. Epub 2003 Sep 3.
CopyrightPublished by The Company of Biologists 2007. This material is protected by a copyright retained by the authors.
CommentsAuthors indicated the probe was the 541 bp fragment of chick ephrin A2 (94-634; L40932).
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
13-18
stage 18
stage 18
stage 18
stage 18
Retina
 
19-24
stage 23
Retina
 
31-34
stage 34
stage 34
stage 34
stage 34
stage 34
stage 34
stage 34
Retina
 

Entry 5

GEISHA IdEPHA2.Jayasena.2005Data SourcePublication
Complete cDNA Template Probeshow
TGGAGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAGGTTCCACCGCG
GGGATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCGCTGCCCGCCGA
GCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGAAGGGCTTCAAA
CGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTTCACCCCCTTCT
CTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTGGACAGACCCTG
CCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTAT
CitationJayasena CS, Flood WD, Koblar SA. High EphA3 expressing ophthalmic trigeminal sensory axons are sensitive to ephrin-A5-Fc: implications for lobe specific axon guidance. Neuroscience. 2005;135(1):97-109.
CopyrightCopyright © 2005 IBRO. Published by Elsevier Ltd. All rights reserved.
CommentsThe complete cDNA template sequence was obtained from the information provided in the publication as described in Jayasena et al., 2005.
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
13-15
stage 13
stage 13
stage 13
stage 15
stage 15
Eye
Head Mesenchyme
Midbrain
mandibular and maxillary processes
mandibular process
all
 
  

Entry 6

GEISHA Idephrin‐A2.Iwamasa.1999Data SourcePublication
Complete cDNA Template Probeshow
CCGCCGCCCGCTGAGCCGCTCCGCTCCGCCGGGACGATGCCGCGCTGGGAGGCGGCCGCGCTGCTCGCCGCGATCGTCG
GCGTCTGCGTGTGGAGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAG
GTTCCACCGCGGGGATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCG
CTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGA
AGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTT
CACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTG
GACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCA
CCAGCAACAACTCGTGCTGCAGCCTTGCAGTGCCGCGTGCCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGG
CTCTTAGTGCCGCGGGACGGACGGACGGGACGG
CitationIwamasa H, Ohta K, Yamada T, Ushijima K, Terasaki H, Tanaka H. Expression of Eph receptor tyrosine kinases and their ligands in chick embryonic motor neurons and hindlimb muscles. Dev Growth Differ. 1999 Dec;41(6):685-98.
CopyrightCopyright © 1999 John Wiley & Sons, Inc.
CommentsInsufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204983.1).
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
25-27
stage 27
stage 27
stage 27
stage 27
Spinal Cord
brachial
cervical
lumbar
thoracic
 Data from NCBI Unigene EST Profile
Unigene ID: Gga.2416

Mouse

Emage
Eurexpress
MGI GXD

Frog

Xenbase

Fruit Fly

BDGP

Zebra Finch

ZEBrA

Zebrafish

ZFIN
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