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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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MECOM
MDS1 and EVI1 complex locus

Gene

Official Symbol	MECOM
Official Full Name	MDS1 and EVI1 complex locus
CGNC ID	7174
also known as	EVI1, MDS1 and EVI1 complex locus protein EVI1\|MDS1 and EVI1 complex locus protein\|ecotropic viral integration site 1, Ecotropic virus integration 1 site protein.
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI

Sequence Information

Genomic
RNA	XM_422804
Polypeptide	XP_422804

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	2122	ENSG00000085276	MIM:165215	MIM:165215
Mouse	14013	ENSMUSG00000027684	All phenotypic alleles (4):Chemically induced(1) Targeted, knock-out(2) Targeted, other(1)	MGI:95457
Xenopus	734157, 100301954			980194
Zebrafish	497407	ENSDARG00000060808	ZDB-GENE-050208-123	ZFIN:ZDB-GENE-050208-123

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	424997
Ensembl Gene
KEGG	gga:424997

Entry 1

GEISHA Id	MECOM.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	5’-TCAGTGCCTTGAAGCCAGAG-3’
Reverse cDNA Template Primer	5’-AATTAACCCTCACTAAAGGCAGGGGAAGCTTTGCAGTTC-3’	cDNA Template Length	753
Complete cDNA Template Probe	show TCAGTGCCTTGAAGCCAGAGGCCAATGAGATGATCCAGTCAATGCCATCCATGTTCAACTTCCGAGCCCCACCAAGTGC TCTGCCTGAGACCCTCCTGCGCAAGGGCAAGGAGCGCTACACCTGCAGATACTGTGGCAAGATATTCCCCAGATCTGCA AACCTAACCCGTCACCTGAGGACGCACACTGGAGAGCAGCCCTACAGATGCAAATACTGTGACAGGTCCTTTAGCATAT CTTCCAACCTGCAGAGACATGTCCGTAATATCCACAATAAAGAGAAGCCATTCAAGTGTCACTTGTGTGACAGGTGTTT TGGTCAACAAACCAATCTTGACAGACATCTAAAAAAGCATGAGAATGGCAACATGTCTGGTACTGCAACATCTTCACCT CACTCAGAACTGGAAAGCACAGGGGCCATCCTAGATGACAAGGAAGATTCTTACTTCACAGAAATTAGGAATTTTATTG GAAACAGCAACCACAACAATCAATCCCCCCGAAACTCAGAGGAGAGAATGAATGGCAGCCACTTTAAGGATGAAAAAGC CATGGTAACCAGCCAGAACTCAGATTTGTTGGATGACGAAGAGGCAGAAGATGAAGTAATGATGGATGAAGAAGACGAA GAGAGTGAAATCGCAGGGAAAACAGTCAAAGAGCCTGTTACAAGTGACATACACGAGGGGACTCCTGAGGAGGATTACG AGGAAACGAGTACTTTGGACATGAACTGCAAAGCTTCCCCTG

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

stage 11

Aorta
Forebrain
Unlabeled

13-18

stage 16

Aorta
Eye
Foregut
Head Mesenchyme
Hindbrain
Outflow Tract
Pharyngeal Arches and Clefts
Pronephros
Sclerotome

19-24

stage 19

stage 20

stage 23

Anterior/Second Heart Field
Aorta
Blood Vessels
Eye
Foregut
Head Mesenchyme
Hindbrain
Leg Mesenchyme
Lung
Midbrain
Nephric Ducts
Neural Plate/Tube
Outflow Tract
Pharyngeal Arches and Clefts
Sclerotome
Sinus Venosus
Splanchnic Mesoderm
Valves
Ventricles
Wing Mesenchyme

25-26

stage 26

Cranial Ganglia
Ear/Otic Placode
Head Mesenchyme
Leg Mesenchyme
Mesonephros
Myotome
Oral Pharynx
Outflow Tract
Pharyngeal Arches and Clefts
Sclerotome
Spinal Cord
Spinal Ganglia and Nerves
Ventricles
Wing Mesenchyme

Entry 2

GEISHA Id	EVI1.Kee.2007	Data Source	Publication
Complete cDNA Template Probe	show AAAAGCCCCCCCCGCCCCCCACGCCGGCATCGGCCGCGGGACTGGCAGCATGCGATCCAAAGGCAGGGCGAGGAAGCTG GCCGCAAGTGATGAATGCCTTTATGAAAGCTTTCCTGAGCTTCCCTTGGAGGATGTGCCAGAAGCTGATGGAGAGGCTG CAAACGCCCAGTGCCCAGCATCTGTCAGCATCCAGGAGCCATCCTCTCCAACCACCTCCAGCGAAGCTTTCACACCAAA GGAGAGCTCTCCTTACAAGGCTACGATATACATTCCCGATGACATTCCCATTCCTTCTGAATTTGAGCTCCGAGAATCC AACATTCCTGGAGCGGGATTAGGAATATGGACCAAAAGGAAGATTGAAGTAGGAGAAAAGTTCGGCCCTTTTGTAGGAG AGCAACGGCCACACCTCAAAGATCCCAGTTACGGTTGGGAGATCTTGGATGAGTTTTGCAATGTGAAGTTCTGCATAGA CGCCAGCCAACCAGATGTAGGAAACTGGCTCAAATACATCAAGTTTGCTGGATGCTACGATCAGCACAACCTTGTTGCA TGTCAGATAAGTGATCAGATATTCTACAGAGTAGTGGCGGACATTGAGCCAGGAGAGGAGCTATTACTGTTCATGAAGA GTGAAGATTATTCACATGAAACAATGGCTCCGGACATTCACGAGGAGCGCCAGTATCGCTGCGAGGACTGCGACCAGCT CTTTGAGTCCAAGGCTGAGCTGGTGGACCACCAGAAGTTCCCCTGCAGCACACCACACTCCGCCTTCTCCATGGTGGAG GAGGACTTCCAGAAAAAGCTTGAGAATGAGAATGACCTTCGGGATGTGCATGAAATCCAGGAGTGTAAAGAGTGTGATC AAGTCTTCCCAGACTTACAAAGCCTGGAGAAGCACATGCTGTCCCACAGCGAGGAGCGGGAGTACAAGTGCGACCAGTG CCCCAAAGCTTTCAACTGGAAGTCCAACTTGATACGGCACCAAATGTCGCATGACAGCGGCAAGCACTACGAGTGTGAG AACTGTGCCAAGGTTTTCACGGACCCCAGCAACCTCCAGAGGCACATTCGTTCCCAGCATGTTGGGGCTCGTGCTCACG CTTGTCCAGAGTGTGGCAAAACCTTTGCCACATCTTCAGGCCTCAAACAGCATAAACACATCCACAGCAGTGTCAAACC CTTTATATGTGAGGTCTGCCATAAATCCTATACTCAGTTTTCAAACCTTTGTCGTCATAAGCGCATGCATGCTGATTGC AGAACCCAAATCAAGTGCAAAGACTGTGGACAAATGTTCAGCACTACGTCTTCCTTAAATAAACACAGGAGATTTTGTG AGGGCAAGAACCATTTTGCAGCAGGAGGATTTTTTGGCCAAGGCATTTCACTTCCTGGAACCCCAGCTATGGATAAAAC GTCCATGGTTAATATGAATCATGCAAATCCGGGCCTTGCTGACTATTTTGGAGCCAATAGGCATCCTGCTGGTCTTACC TTTCCAACAGCTCCTGGATTTTCTTTTAGCTTCCCCGGTCTGTTTCCTTCAGGCTTGTACCACAGGCCACCTTTGCTAC CTACTAGCTCTCCTGTTAAAGGACTACCAAGCGCAGATCAGACAAACAAAAGTCAAAGTCCCCTCATGACCAACCCTCA GATACTGCCAGCCACCCAGGATATTTTGAAGGCACTAAATAAGCAACCATCTGTAGGGGAAAATAAGCCAGTGGAGCTT CAACCAGAGAGGTCCTCTGAAGAGAGGCCGCATGAGAAACTCAGTGACCAGTCAGAGAGTAGTGACCTTGACGATGTCA GTACCCCCAGTGGCAGTGACCTGGAAACCACCTCTGGCTCTGATCTGGAAAGTGACATTGAAAGTGAGAAAGAGAAATT TAAAGAAAATGGTAAAATGTTCAAAGACAAAGTAAGCTCTCTGCAGAGTTTGGCTTCAATAAATAATAAGAAAGAACAC AGCAATCATTCCATTTTCTCACCATCTTTAGAGGAGCAGACTGCGGTGTCAGGAGCGGTGAATGATTCTATAAAGGCTA TTGCTTCTATTGCTGAAAAATACTTTGGTTCAACAGGACTTGTGGGGCTGCAAGACAAAAAAGTTGGAGCCTTACCTTA CCCTTCCATGTTTCCCCTCCCATTTTTTCCAGCATTCTCTCAATCAATGTATCCATTTCCTGATAGAGACTTGAGACCG TTACCCTTGAAAGTGGAGCCCCAATCACCCAGTGAACTCAAGAAGATCCCAAAGGGAAGCTCTGAGTCTCCCTTTGACC TCACCACTAAGCGTAAGGAGGAGAAGCCCGTTACTCCCATACCCTCAAAGCCAGCAGCTCCGCCTGCGGCAAGCCAGGA CCAGCCTCTAGATCTGAGCATGGGCAGTAGAAGTCGAGCCAGCAGCACAAAACAGGCCGAGCCCCGGAAAAACCACGTC TTTGGTGAGAAGAAAGGAGGTGACCTCGAGCAGAGGAAAACGTCGGAGGCCTCTTTGCAGCACGCCCGACCAACTCCCT TCTTTATGGATCCCATTTACAGAGTAGAGAAAAGAAAGTTAACTGACCCACTTGAAGCTTTAAAAGAGAAATACTTGAG ACCTTCCCCGGGATTCTTGTTTCATCCACAATTCCAATTGCCTGATCAAAGAATTTGGATGTCAGCTATAGAGAACATG GCCGAAAAGCTGGAGAGTTTCAGTGCCTTGAAGCCAGAGGCCAATGAGATGATCCAGTCAATGCCATCCATGTTCAACT TCCGAGCCCCACCAAGTGCTCTGCCTGAGACCCTCCTGCGCAAGGGCAAGGAGCGCTACACCTGCAGATACTGTGGCAA GATATTCCCCAGATCTGCAAACCTAACCCGTCACCTGAGGACGCACACTGGAGAGCAGCCCTACAGATGCAAATACTGT GACAGGTCCTTTAGCATATCTTCCAACCTGCAGAGACATGTCCGTAATATCCACAATAAAGAGAAGCCATTCAAGTGTC ACTTGTGTGACAGGTGTTTTGGTCAACAAACCAATCTTGACAGACATCTAAAAAAGCATGAGAATGGCAACATGTCTGG TACTGCAACATCTTCACCTCACTCAGAACTGGAAAGCACAGGGGCCATCCTAGATGACAAGGAAGATTCTTACTTCACA GAAATTAGGAATTTTATTGGAAACAGCAACCACAACAATCAATCCCCCCGAAACTCAGAGGAGAGAATGAATGGCAGCC ACTTTAAGGATGAAAAAGCCATGGTAACCAGCCAGAACTCAGATTTGTTGGATGACGAAGAGGCAGAAGATGAAGTAAT GATGGATGAAGAAGACGAAGAGAGTGAAATCGCAGGGAAAACAGTCAAAGAGCCTGTTACAAGTGACATACACGAGGGG ACTCCTGAGGAGGATTACGAGGAAACGAGTACTTTGGACATGAACTGCAAAGCTTCCCCTGGCAGGTATAAAGAGGAGG AATATAATAAGACTGGACTTTCAGCTTTGGACCACATAAGGCACTTCACAGATAGCCTCAAAATGAGGAAAATGGAAGA AAATCAATATAGTGAAGCTGAATTGGCAGCTTTCAATACTTCCCAGCTGCCAGAGGACCTAAAACAACCATTGTACAGG AAATCCAAATCCCAGGCATACGCCATGATGCTCTCTCTCTCTGACAAGGACTCCCTTCATTCTGCATCCCATAATTCTC CCAATATGTGGCACAGCATGGCGAGAGCTGCTGCAGAATCCAGCGCCATCCAATCCATCAGTCACGTATGACATTGTGA AGTCTTACCAAGAATGGGACCAAGTCCAAACCAGTAGCATGGCTCTTTCATATATGACTATTTAAAAGACTGCTGAGCA GAATGCCTTATAAATCTGCAGGGTCACTCATTTAAAGTCTGGTGACCTTAAACTGAATAATTTTAAAAAGAAAGAAACT ATTTATTCTCAATATTTTGTTTTGCACAGCAAAGGCAGCTGCTGACTTCTGGAGGATCAATGCGACTTAAAATAAAGAG TTTCAGTGGATTATGTAAACCAGGGCCGGGAAAAAGTGTCTTCCAGTTCACCCGGCTTTGAGAGGCAGGGGCAAAAGGG TTAAGACTGCATTGATACACACATGGGCACTCCTTTAAAGTAGTAACTGAATTGATTTTCTTTTTGTATAAAATAGTTT GACACAGGATTGGGAACAGTTGCTTTTATGTACAAATCTCTTCATTAAAGCTTTATGCATTTTAGCCCTTCAAAGAAAG AAAGAAAATAGTTATATGAAATGCACACTATCCATCATAAATGTAGAAAGAAGTAACAGGGAATATTTTGTTCTCTCCG TAATTCTTTTGCCTTCTCAACTGCATTGTTAAAAAAAAAAGGAAAAATGAGAAAAAAAAAAGAAAAAAAAAAAAAGGAA CTATGAGAAACAATACATACG
Citation	Yun Kee, Byung Joon Hwang, Paul W. Sternberg, Marianne Bronner-Fraser. Evolutionary conservation of cell migration genes: from nematode neurons to vertebrate neural crest. Genes Dev. 2007 February 15; 21(4): 391396.
Copyright	Copyright © 2007, Cold Spring Harbor Laboratory Press
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # XM_422804.3).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-11

stage 11

Forebrain
Hindbrain
Midbrain
Neural Crest
Neural Plate/Tube
Spinal Cord

Entry 3

GEISHA Id	Evi1.Celá.2013	Data Source	Publication
Complete cDNA Template Probe	show GCGCAGATCAGACAAACAAAAGTCAAAGTCCCCTCATGACCAACCCTCAGATACTGCCAGCCACCCAGGATATTTTGAA GGCACTAAATAAGCAACCATCTGTAGGGGAAAATAAGCCAGTGGAGCTTCAACCAGAGAGGTCCTCTGAAGAGAGGCCG CATGAGAAACTCAGTGACCAGTCAGAGAGTAGTGACCTTGACGATGTCAGTACCCCCAGTGGCAGTGACCTGGAAACCA CCTCTGGCTCTGATCTGGAAAGTGACATTGAAAGTGAGAAAGAGAAATTTAAAGAAAATGGTAAAATGTTCAAAGACAA AGTAAGCTCTCTGCAGAGTTTGGCTTCAATAAATAATAAGAAAGAACACAGCAATCATTCCATTTTCTCACCATCTTTA GAGGAGCAGACTGCGGTGTCAGGAGCGGTGAATGATTCTATAAAGGCTATTGCTTCTATTGCTGAAAAATACTTTGGTT CAACAGGACTTGTGGGGCTGCAAGACAAAAAAGTTGGAGCCTTACCTTACCCTTCCATGTTTCCCCTCCCATTTTTTCC AGCATTCTCTCAATCAATGTATCCATTTCCTGATAGAGACTTGAGACCGTTACCCTTGAAAGTGGAGCCCCAATCACCC AGTGAACTCAAGAAGATCCCAAAGGGAAGCTCTGAGTCTCCCTTTGACCTCACCACTAAGCGTAAGGAGGAGAAGCC
Citation	Celá P, Balková SM, Bryjová A, Horáková D, Míšek I, Richman JM, Buchtová M. Expression, function and regulation of Evi-1 during embryonic avian development. Gene Expr Patterns. 2013 Dec;13(8):343-53. Epub 2013 Jul 3. DIO: 10.1016/j.gep.2013.06.002.
Copyright	Copyright © 2013 Elsevier B.V.
Comments	Authors indicated that probes were synthesized from three different regions (E2, E4 and E8) of chicken Evi1 (XM_422804). The sequence of probe provided here was from E4 region. The probe was amplified by PCR using the following primers: 5'-GCGCAGATCAGACAAACAAA-3' and 5'-GGCTTCTCCTCCTTACGCTT-3'.