Geisha

Anterior Intestinal Portal
Endoderm
Forebrain
Head Mesenchyme
Heart Tube
Intermediate Mesoderm
Isthmus/MHB
Lateral Plate Mesoderm
Neural Plate/Tube
Paraxial Mesoderm
Pharyngeal Arches and Clefts
Primitive Streak
Surface Ectoderm

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

Cardiogenic mesoderm
Dorsal
Dorsal view. Anterior Ectoderm
Ventral View.

13-18

stage 13

stage 14

stage 15

Anterior/Second Heart Field
Eye
Face Mesenchyme
Forebrain
Intermediate Mesoderm
Isthmus/MHB
Midbrain
Myotome
Paraxial Mesoderm
Pharyngeal Arches and Clefts
Primitive Streak
Somites
Tail

Dorsal view.

19-24

stage 19

stage 20

stage 22

stage 23

Head Mesenchyme
Leg AER
Nasal Placode/Nerve
Pharyngeal Arches and Clefts
Somites
Wing AER

stage 25

Head Mesenchyme
Leg AER
Nasal Placode/Nerve
Pharyngeal Arches and Clefts
Somites
Wing AER

Entry 2

GEISHA Id	Fgf8.Mikawa.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Mikawa, T, Poh, A, Kelly, K, Ishii, Y, Reese, D. Induction and Patterning of the Primitive Streak, an Organizing Center of Gastrulation in the Amniote. Dev Dyn. 2004 Mar;229(3):422-32.
Copyright	Copyright © 2004 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767).

Stage

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1-3

stage 2

stage 3

4-6

stage 4

stage 6

Fgf8 (bluish purple), Chordin (red)

Entry 3

GEISHA Id	Fgf8.Narita.2005	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Narita T, Sasaoka S, Udagawa K, Ohyama T, Wada N, Nishimatsu S, Takada S, Nohno T. Wnt10a is involved in AER formation during chick limb development. Dev Dyn. 2005 Jun;233(2):282-7.
Copyright	Wiley-Liss, Inc. 2005
Comments	compared to Wnt10a and 3a in the paper.

Stage

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13-18

stage 18

Forebrain
Pharyngeal Arches and Clefts
Somites
Wing AER

19-21

stage 21

Wing AER

Entry 4

GEISHA Id	Fgf8.Chapman.2002	Data Source	Publication
Complete cDNA Template Probe	show GTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTCAGTCCCCACCTAATTTTACACAGCATGTGA GGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCACCTACCAGCTGTACAGCCGGACCAGCGGGAA GCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGACGGGGATGTGCACGCCAAGCTCATCGTCGAG ACCGACACCTTTGGGAGCCGTGTGCGCATCAAAGGGGCGGCCACGGGGTTCTACATCTGCATGAACAAGAAGGGGAAAC TGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACCGAGATCGTGCTGGAGAACAACTACACGGCGCTGCA GAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGCCCGCGCAAGGGCTCCAAGACCCGGCAGCAC CAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCACCGAGCCCCACCGGCGCTTCGAGTTCCTCA ACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCAGGGTGCGCCCATGACGCGTGCCCCTCGGTGG ACTGACGTTGAGACATTGTGATAGGGTTACTAAAGGAAAAAAAAAACCCAACGGGGGATGGGGGGAGGATACGAGAACT ACATGCTCTAGTTGTTGGTAATGTGGGGGTTGTTCTGTTTTTACAAAAAAAAAAACCAAACAAACAAAAAAAAAAAAAA AAAACTCGAG
Citation	Chapman SC, Schubert FR, Schoenwolf GC, Lumsden A. Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos. Dev Biol. 2002 May 1;245(1):187-99.
Copyright	2002 Elsevier Science (USA). All rights reserved.
Comments	Author said U41467 is probe.

Stage

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X-XIV

stage XIV

Kohler's Sickle

1-3

stage 2

stage 3

Hensen's Node
Primitive Streak

4-6

stage 4

stage 6

Entry 5

GEISHA Id	Fgf8.Karabagli.2002	Data Source	Publication
Complete cDNA Template Probe	show GTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTCAGTCCCCACCTAATTTTACACAGCATGTGA GGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCACCTACCAGCTGTACAGCCGGACCAGCGGGAA GCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGACGGGGATGTGCACGCCAAGCTCATCGTCGAG ACCGACACCTTTGGGAGCCGTGTGCGCATCAAAGGGGCGGCCACGGGGTTCTACATCTGCATGAACAAGAAGGGGAAAC TGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACCGAGATCGTGCTGGAGAACAACTACACGGCGCTGCA GAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGCCCGCGCAAGGGCTCCAAGACCCGGCAGCAC CAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCACCGAGCCCCACCGGCGCTTCGAGTTCCTCA ACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCAGGGTGCGCCCATGACGCGTGCCCCTCGGTGG ACTGACGTTGAGACATTGTGATAGGGTTACTAAAGGAAAAAAAAAACCCAACGGGGGATGGGGGGAGGATACGAGAACT ACATGCTCTAGTTGTTGGTAATGTGGGGGTTGTTCTGTTTTT
Citation	Karabagli H, Karabagli P, Ladher RK, Schoenwolf GC. Comparison of the expression patterns of several fibroblast growth factors during chick gastrulation and neurulation. Anat Embryol (Berl). 2002 Oct;205(5-6):365-70. Epub 2002 Sep 4.
Copyright	Copyright © Springer-Verlag 2002

Stage

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4-6

stage 4

Early Mesoderm
Primitive Streak

7-11

stage 9

stage 11

Ear/Otic Placcode
Endoderm
Forebrain
Primitive Streak
Tail

Entry 6

GEISHA Id	Fgf8.Karabagli.2002b	Data Source	Publication
Complete cDNA Template Probe	show GTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTCAGTCCCCACCTAATTTTACACAGCATGTGA GGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCACCTACCAGCTGTACAGCCGGACCAGCGGGAA GCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGACGGGGATGTGCACGCCAAGCTCATCGTCGAG ACCGACACCTTTGGGAGCCGTGTGCGCATCAAAGGGGCGGCCACGGGGTTCTACATCTGCATGAACAAGAAGGGGAAAC TGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACCGAGATCGTGCTGGAGAACAACTACACGGCGCTGCA GAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGCCCGCGCAAGGGCTCCAAGACCCGGCAGCAC CAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCACCGAGCCCCACCGGCGCTTCGAGTTCCTCA ACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCAGGGTGCGCCCATGACGCGTGCCCCTCGGTGG ACTGACGTTGAGACATTGTGATAGGGTTACTAAAGGAAAAAAAAAACCCAACGGGGGATGGGGGGAGGATACGAGAACT ACATGCTCTAGTTGTTGGTAATGTGGGGGTTGTTCTGTTTTT
Citation	Karabagli H, Karabagli P, Ladher RK, Schoenwolf GC. Survey of fibroblast growth factor expression during chick organogenesis. Anat Rec. 2002 Sep 1;268(1):1-6.
Copyright	Copyright © 2002 Wiley-Liss Inc.

Stage

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stage 19

Eye
Isthmus/MHB
Myotome
Nasal Placcode/Nerve
Pharyngeal Arches and Clefts
Primitive Streak
Somites
Wing AER

Entry 7

GEISHA Id	FGF8.Wu.2006	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Wu P, Jiang TX, Shen JY, Widelitz RB, Chuong CM. Morphoregulation of avian beaks: comparative mapping of growth zone activities and morphological evolution. Dev Dyn. 2006 May;235(5):1400-12.
Copyright	Copyright © 2006 Wiley-Liss, Inc.

Stage

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19-23

stage 20

stage 23

Eye
Nasal Placcode/Nerve

FNM Ectoderm
FNM ectoderm

Entry 8

GEISHA Id	FGF8.Hurtado.2006	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Hurtado R, Mikawa T. Enhanced sensitivity and stability in two-color in situ hybridization by means of a novel chromagenic substrate combination. Dev Dyn. 2006 Oct;235(10):2811-6.
Copyright	Copyright © 2006 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

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13-18

stage 18

stage 19

Isthmus/MHB
Surface Ectoderm

Entry 9

GEISHA Id	Fgf8.Goodnough.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Goodnough LH, Brugmann SA, Hu D, Helms JA. Stage-dependent craniofacial defects resulting from Sprouty2 overexpression. Dev Dyn. 2007 Jul;236(7):1918-28.
Copyright	Copyright © 2007 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI(NM_001012767).

Stage

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7-12

stage 10

13-18

stage 17

Isthmus/MHB
Pharyngeal Arches and Clefts
Surface Ectoderm
Telencephalon

19-20

stage 20

Nasal Placcode/Nerve
Surface Ectoderm
Telencephalon

Entry 10

GEISHA Id	FGF8.Aglyamova.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Aglyamova GV, Agarwala S. Gene expression analysis of the hedgehog signaling cascade in the chick midbrain and spinal cord. Dev Dyn. 2007 May;236(5):1363-73
Copyright	Copyright © 2007 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

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7-12

stage 10

13-16

stage 16

Forebrain
Hindbrain
Isthmus/MHB
Midbrain
Neural Plate/Tube

Entry 11

GEISHA Id	FGF8.Gimeno.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Gimeno L, Martinez S. Expression of chick Fgf19 and mouse Fgf15 orthologs is regulated in the developing brain by Fgf8 and Shh. Dev Dyn. 2007 Aug;236(8):2285-97.
Copyright	Copyright © 2007 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

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19-24

stage 19

stage 20

stage 22

stage 24

25-26

stage 26

Isthmus/MHB
Neural Plate/Tube

Entry 12

GEISHA Id	Fgf8.vonFrowein.2006	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_001012767).

Stage

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19-22

stage 22

Telencephalon

Entry 13

GEISHA Id	FGF8.Kruithof.2006	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Kruithof BP, van Wijk B, Somi S, Kruithof-de Julio M, Pérez Pomares JM, Weesie F, Wessels A, Moorman AF, van den Hoff MJ. BMP and FGF regulate the differentiation of multipotential pericardial mesoderm into the myocardial or epicardial lineage. Dev Biol. 2006 Jul 15;295(2):507-22. Epub 2006 Apr 3.
Copyright	Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_001012767).

Stage

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13-16

stage 16

Splanchnic Mesoderm

peri-proepicardium

Entry 14

GEISHA Id	Fgf8.Sanchez-Calderon.2007	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767 ).

Stage

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13-18

stage 14

stage 15

Ear/Otic Placcode
Sensory Organs

Figure 1. includes HH stage 14 and 15. Also, includes comparison of Fgf19 expression to Fgf8, Fgf10 and Otx2 expression in chick embryos.

19-24

stage 20

stage 24

Ear/Otic Placcode
Sensory Organs

Figure 2 includes comparison of Fgf19 expression to Fgf8, Otx2 and Bmp4 expression in stage 20 chick embryos.
Figure includes comparison of Fgf19 expression to Fgf8, Otx2 and Bmp4 expression in stage 24 chick embryos.
bp, basilar papilla; ml macula lagenda
ml, macula lagenda
mu, macula utricula; ms macula sacculi

25-27

stage 27

Ear/Otic Placcode

Figure includes comparison of Fgf19 expression to Fgf8 and Bmp4 expression in stage 27 chick embryos.
macula lagenda
ml, macula lagenda; bp, basilar papilla

Entry 15

GEISHA Id	Fgf8.Ha.2003	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Ha AS, Riddle RD. cBlimp-1 expression in chick limb bud development. Gene Expr Patterns. 2003 Jun;3(3):297-300.
Copyright	Reprinted from Gene Expr Patterns, Volume 3, Issue 3, Alice S. Ha and Robert D. Riddle, cBlimp-1 expression in chick limb bud development, Pages 297-300, Copyright 2003, with permission from Elsevier. Copyright © 2003 Elsevier Science B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_001012767).

Stage

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Location (click to highlight)

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13-18

stage 17

Wing AER

19-20

stage 19

stage 20

Leg AER
Wing AER

Entry 16

GEISHA Id	Fgf8.Badde.2005	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Badde A, Bumsted-O'Brien KM, Schulte D. Chick receptor protein tyrosine phosphatase lambda/psi (cRPTPlambda/cRPTPpsi) is dynamically expressed at the midbrain-hindbrain boundary and in the embryonic neural retina. Gene Expr Patterns. 2005 Aug;5(6):786-91.
Copyright	Copyright © 2005 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-16

stage 15

stage 16

Ectoderm
Forebrain
Pharyngeal Arches and Clefts
Surface Ectoderm

Entry 17

GEISHA Id	FGF8.Shigetani.2000	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Shigetani Y, Nobusada Y, Kuratani S. (2000). Ectodermally derived FGF8 defines the maxillomandibular region in the early chick embryo: epithelial-mesenchymal interactions in the specification of the craniofacial ectomesenchyme. Dev Biol. 2000 Dec 1;228(1):73-85.
Copyright	Reprinted with permission from Elsevier. Copyright © 2000 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

stage 11

Anterior Neuropore
Maxillomandibular Domain
Pharyngeal
Ventral

13-18

stage 13

stage 14

stage 17

stage 18

Ectoderm
Forebrain
Isthmus/MHB
Pharyngeal Arches and Clefts
Surface Ectoderm

Anterior Neuropore
Double Label
Figure 2 compares expression patterns of Fgf8 and Bmp4 in early chick embryos at HH stages 10, 11-14 and 18.
Mandibular Arch
Maxillary Process
Maxillomandibular Domain
Pharyngeal Slit Ectoderm

19-24

stage 19

stage 23

stage 24

Ectoderm
Pharyngeal Arches and Clefts

Double Label
Figure 3 compares expression patterns of mesenchymally expressed homeobox genes (Msx1, Fgf8, Dlx1, and Barx1) in chick embryo at HH stages 13, 15, 17, 19, 20, 23 and 24.
Mandibular Arch
Maxillary Process
Pharyngeal Slit Ectoderm

Entry 18

GEISHA Id	Fgf8.Maden.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Maden M, Blentic A, Reijntjes S, Seguin S, Gale E, Graham A. (2007). Retinoic acid is required for specification of the ventral eye field and for Rathke's pouch in the avian embryo. Int J Dev Biol. 2007;51(3):191-200.
Copyright	"Reprinted from Int. J. Dev. Biol., 51, Maden M, Blentic A, Reijntjes S, Seguin S, Gale E, Graham A, Retinoic acid is required for specification of the ventral eye field and for Rathke's pouch in the avian embryo, 191-200, Copyright (2007), with permission from Copyright © 2007 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_001012767). Images are quail, but sequence and map shown are chick.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-20

stage 20

Eye
Forebrain
Isthmus/MHB
Oral Pharynx
Pharyngeal Arches and Clefts
Pituitary (Rudiment)

Rathke's pouch
quail

Entry 19

GEISHA Id	Fgf8.Christ.2002	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Christ B, Brand-Saberi B. Limb muscle development. Int J Dev Biol. 2002;46(7):905-14.
Copyright	"Reprinted from Int. J. Dev. Biol., 46, Christ B, Brand-Saberi B, Limb muscle development, 905-14, Copyright (2002), with permission from Copyright © 2002 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 22

Somites
Wing AER

double labeled with MyoD

31-44

stage 44

Limb Buds
Somites

Compare with other genes

Entry 20

GEISHA Id	Fgf8.Chapman.2008	Data Source	Direct Submission
Complete cDNA Template Probe	show GTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTCAGTCCCCACCTAATTTTACACAGCATGTGA GGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCACCTACCAGCTGTACAGCCGGACCAGCGGGAA GCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGACGGGGATGTGCACGCCAAGCTCATCGTCGAG ACCGACACCTTTGGGAGCCGTGTGCGCATCAAAGGGGCGGCCACGGGGTTCTACATCTGCATGAACAAGAAGGGGAAAC TGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACCGAGATCGTGCTGGAGAACAACTACACGGCGCTGCA GAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGCCCGCGCAAGGGCTCCAAGACCCGGCAGCAC CAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCACCGAGCCCCACCGGCGCTTCGAGTTCCTCA ACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCAGGGTGCGCCCATGACGCGTGCCCCTCGGTGG ACTGACGTTGAGACATTGTGATAGGGTTACTAAAGGAAAAAAAAAACCCAACGGGGGATGGGGGGAGGATACGAGAACT ACATGCTCTAGTTGTTGGTAATGTGGGGGTTGTTCTGTTTTTACAAAAAAAAAAACCAAACAAACAAAAAAAAAAAAAA AAAACTCGAG
Comments	Images are using a chick probe unless noted. Mouse probe NM_010205.1 was used before the chick probe became available. Images Provided by Susan Chapman.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Anterior Intestinal Portal
Forebrain
Head Mesenchyme
Isthmus/MHB
Lateral Plate Mesoderm
Primitive Streak

Mouse probe

7-12

stage 7

stage 8

stage 9

stage 11

Mouse probe
anterior neuropore

13-17

stage 17

Forebrain
Isthmus/MHB
Pharyngeal Arches and Clefts
Primitive Streak
Tail

Mouse probe

Entry 21

GEISHA Id	FGF8.Vieira.2005	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Vieira C, Garda AL, Shimamura K, Martinez S. Thalamic development induced by Shh in the chick embryo. Dev Biol. 2005 Aug 15;284(2):351-63.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 25

Forebrain
Isthmus/MHB

mantle layer of the alar plate
zona limitans intrathalamica

Entry 22

GEISHA Id	Fgf8.Canning.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Canning CA, Lee L, Irving C, Mason I, Jones CM. Sustained interactive Wnt and FGF signaling is required to maintain isthmic identity. Dev Biol. 2007 May 1;305(1):276-86. Epub 2007 Feb 16.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #NM_001012767.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 8

stage 9

stage 10

stage 11

stage 12

Isthmus/MHB
Neural Plate/Tube
Tail

Double label, FGF8 in red
Double label, FGF8 red

13-16

stage 16

Forebrain
Isthmus/MHB
Neural Plate/Tube
Pharyngeal Arches and Clefts

Entry 23

GEISHA Id	Fgf8.Haworth.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Haworth KE, Wilson JM, Grevellec A, Cobourne MT, Healy C, Helms JA, Sharpe PT, Tucker AS. Sonic hedgehog in the pharyngeal endoderm controls arch pattern via regulation of Fgf8 in head ectoderm. Dev Biol. 2007 Mar 1;303(1):244-58.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	As information provided is insufficient to verify exact sequence used to synthesize the probe, the sequence below was obtained from NCBI (Acc# NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 8

stage 10

stage 11

Isthmus/MHB
Neural Plate/Tube
Pharyngeal Arches and Clefts
Surface Ectoderm

13-18

stage 14

Isthmus/MHB
Nasal Placcode/Nerve
Pharyngeal Arches and Clefts
Surface Ectoderm

Oral Ectoderm

19-22

stage 20

stage 22

Nasal Placcode/Nerve
Pharyngeal Arches and Clefts
Surface Ectoderm

oral ectoderm

Entry 24

GEISHA Id	Fgf8.Firnberg.2002	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Firnberg N, Neubüser A (2005).FGF signaling regulates expression of Tbx2, Erm, Pea3, and Pax3 in the early nasal region. Dev Biol. 2002 Jul 15;247(2):237-50.
Copyright	"Reprinted from Developmental Biology, 247(2), Firnberg N, Neubüser A, FGF signaling regulates expression of Tbx2, Erm, Pea3, and Pax3 in the early nasal region, 237-50., Copyright (2002), with permission from Elsevier". Copyright © (2002) Elsevier B.V. All rights reserved.
Comments	According to authors, "The plasmids used to prepare the antisense riboprobes employed in this study have previously been described (refs given) As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Forebrain
Nasal Placcode/Nerve
Surface Ectoderm

31-44

stage 44

Ectoderm
Face Mesenchyme
Forebrain

Entry 25

GEISHA Id	Fgf8.Edom-Vovard.2002	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Edom-Vovard F, Schuler B, Bonnin MA, Teillet MA, Duprez D (2002). Fgf4 positively regulates scleraxis and tenascin expression in chick limb tendons. Dev Biol. 2002 Jul 15;247(2):351-66.
Copyright	"Reprinted from Developmental Biology, 247(2), Edom-Vovard F, Schuler B, Bonnin MA, Teillet MA, Duprez D, Fgf4 positively regulates scleraxis and tenascin expression in chick limb tendons., Copyright (2002), with permission from Elsevier". Copyright © (2002) Elsevier B.V. All rights reserved.
Comments	According to authors , "The digoxigeninlabelled mRNA probes were prepared as described: cFgf4, mFgf4, and MyoD (Edom-Vovard et al., 2001b), and scleraxis and Fgf8 (Edom-Vovard et al., 2001a)." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

31-44

stage 35

stage 44

Wing Muscles

Entry 26

GEISHA Id	FGF8.Streit.2000	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Streit A, Berliner AJ, Papanayotou C, Sirulnik A, Stern CD. Initiation of neural induction by FGF signalling before gastrulation.Nature. 2000 Jul 6;406(6791):74-8.
Copyright	Copyright Nature Publishing Group. Nature Publishing Group is a division of Macmillan Publishers Limited, of Brunel Road, Houndmills, Basingstoke, Hampshire, RG21 6XS. Registered Number 785998 England.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage X

stage XII

Area Pellucida
Hypoblast
Kohler's Sickle

Stage XII

31-44

stage 44

Area Pellucida

Entry 27

GEISHA Id	FGF8.Streit.1999	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Streit A, Stern CD. Establishment and maintenance of the border of the neural plate in the chick: involvement of FGF and BMP activity. Mech Dev. 1999 Apr;82(1-2):51-66.
Copyright	Copyright © 1999 Elsevier Science Ireland Ltd. All rights reserved
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Hensen's Node
Primitive Streak

7-8

stage 8

Anterior Intestinal Portal
Primitive Streak

segmental plate

Entry 28

GEISHA Id	FGF8.Wilm.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Wilm B, James RG, Schultheiss TM, Hogan BL (2004). The forkhead genes, Foxc1 and Foxc2, regulate paraxial versus intermediate mesoderm cell fate. Dev Biol. 2004 Jul 1;271(1):176-89.
Copyright	Copyright © 2004 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

Intermediate Mesoderm
Paraxial Mesoderm
Somites

Neural Folds
Presomitic Mesoderm

Entry 29

GEISHA Id	FGF8.Huang.2003	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Huang R, Stolte D, Kurz H, Ehehalt F, Cann GM, Stockdale FE, Patel K, Christ B. Ventral axial organs regulate expression of myotomal Fgf-8 that influences rib development. Dev Biol. 2003 Mar 1;255(1):30-47.
Copyright	Copyright © 2003 Elsevier Science (USA) All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 16

Myotome
Somites

19-21

stage 21

Leg AER
Myotome
Somites
Wing AER

Entry 30

GEISHA Id	FGF8.LeDouarin.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Le Douarin NM, Creuzet S, Couly G, Dupin E. Neural crest cell plasticity and its limits. Development. 2004 Oct;131(19):4637-50.
Copyright	Published by The Company of Biologists 2004
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-15

stage 15

Ectoderm
Forebrain
Isthmus/MHB
Pharyngeal Arches and Clefts
Surface Ectoderm

Nasofrontal Ectoderm
Neuroectoderm

Entry 31

GEISHA Id	FGF8.Bailey.2006	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Bailey AP, Bhattacharyya S, Bronner-Fraser M, Streit A. Lens specification is the ground state of all sensory placodes, from which FGF promotes olfactory identity. Dev Cell. 2006 Oct;11(4):505-17.
Copyright	Copyright © 2006, Elsevier
Comments	Insufficient information provided in publication to verify exact sequence to synthesize probe. Sequence below was obtained from NCBI (NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-10

stage 8

stage 10

Ectoderm
Nasal Placcode/Nerve
Neural Plate/Tube
Primitive Streak

Entry 32

GEISHA Id	FGF8.Lopez-Sanchez.2009	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Lopez-Sanchez C, Garcia-Masa N, Gañan CM, Garcia-Martinez V. Movement and commitment of primitive streak precardiac cells during cardiogenesis. Int J Dev Biol. 2009;53(8-10):1445-55.
Copyright	Reprinted from Int. J. Dev. Biol. Copyright (2009), with permission from UBC Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_001012767.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Early Endoderm
Endoderm
Primitive Streak

7-8

stage 7

stage 8

Early Endoderm
Primitive Streak
Splanchnic Mesoderm

Entry 33

GEISHA Id	FGF8.Olivera-Martinez.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Olivera-Martinez I, Storey KG., Wnt signals provide a timing mechanism for the FGF-retinoid differentiation switch during vertebrate body axis extension. Development. 2007 Jun;134(11):2125-35.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. Permission granted by Dr. Storey.
Comments	As insufficient information is provided in the publication to verify the exact sequence used to synthesize the probe, the sequence below was obtained from NCBI (acc# NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

Neural Plate/Tube
Primitive Streak
Tail

Entry 34

GEISHA Id	FGF8.DiezdelCorral.2002	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Diez del Corral R, Breitkreuz DN, Storey KG., Onset of neuronal differentiation is regulated by paraxial mesoderm and requires attenuation of FGF signalling. Development. 2002 Apr;129(7):1681-91.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. Permission granted by Dr. Storey
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-9

stage 9

Neural Plate/Tube

Entry 35

GEISHA Id	FGF8.Berthoud.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Berthoud VM, Singh R, Minogue PJ, Ragsdale CW, Beyer EC. Highly restricted pattern of connexin36 expression in chick somite development. Anat Embryol (Berl). 2004 Nov;209(1):11-8.
Copyright	© Springer-Verlag 2004
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

19-24

stage 24

Myotome

Entry 36

GEISHA Id	FGF8.Hardy.2011	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Hardy KM, Yatskievych TA, Konieczka J, Bobbs AS, Antin PB. FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression. BMC Dev Biol. 2011 Mar 21;11:20.
Copyright	© 2011 Hardy et al; licensee BioMed Central Ltd.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Epiblast
Primitive Streak

preingression epiblast

Entry 37

GEISHA Id	FGF8.Fuchs.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Fuchs A, Inthal A, Herrmann D, Cheng S, Nakatomi M, Peters H, Neubüser A. Regulation of Tbx22 during facial and palatal development. Dev Dyn. 2010 Nov;239(11):2860-74.
Copyright	© 2010 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Face Mesenchyme

19-24

stage 19

stage 23

Face Mesenchyme

mx, maxilla ; np, nasal placode

25-26

stage 26

Face Mesenchyme

Entry 38

GEISHA Id	FGF8.Parkinson.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Parkinson N, Collins MM, Dufresne L, Ryan AK. Expression patterns of hormones, signaling molecules, and transcription factors during adenohypophysis development in the chick embryo. Dev Dyn. 2010 Apr;239(4):1197-210.
Copyright	Copyright © 2010 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 21

Diencephalon
Pituitary (Rudiment)

oe, oral epithelium

25-30

stage 28

Pituitary (Rudiment)

31-38

stage 38

Pituitary (Rudiment)

Entry 39

GEISHA Id	FGF8.Ferrer-Vaquer.2008	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Ferrer-Vaquer A, Maurey P, Werzowa J, Firnberg N, Leibbrandt A, Neubüser A. Expression and regulation of HTRA1 during chick and early mouse development. Dev Dyn. 2008 Jul;237(7):1893-900.
Copyright	© 2008 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Ectoderm
Forebrain
Nasal Placode/Nerve

Entry 40

GEISHA Id	FGF8.Halilagic.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Halilagic A, Ribes V, Ghyselinck NB, Zile MH, Dollé P, Studer M. Retinoids control anterior and dorsal properties in the developing forebrain. Dev Bio 2007 Mar 1;303(1):244-58
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_001012767.1). Images are quail, but sequence and map shown are chick.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 14

Eye

quail embryo

stage 19

Anterior Neuropore
Eye
Nasal Placode/Nerve
Pharyngeal Arches and Clefts

anr, anterior neural ridge
double hybridized with FGF8(red) and BMP4(blue)
quail embryo

Entry 41

GEISHA Id	FGF8.Mao.2009	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Mao J, McGlinn E, Huang P, Tabin CJ, McMahon AP. Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb. Dev Cell. 2009 Apr;16(4):600-6.
Copyright	don't have permission yet
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-17

stage 17

Wing Ectoderm

ST 17/18
double-hybridization: SHH expression (black arrow), FGF8 expression (white arrow)

Entry 42

GEISHA Id	FGF8.Maier.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Maier, E, von Hofsten, J, Nord, H, Fernandes, M, Paek, H, Hebert, JM, Gunhaga, L, Opposing Fgf and Bmp activities regulate the specification of olfactory sensory and respiratory epithelial cell fates. Development. 2010 May 15; 137(10): 16011611.
Copyright	Copyright © 2010. Held by the authors. Requested

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 14

Nasal Placode/Nerve

19-22

stage 22

Nasal Placode/Nerve

Entry 43

GEISHA Id	FGF8.Tossell.2011	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Tossell K, Kiecker C, Wizenmann A, Lang E, Irving C. Notch signalling stabilises boundary formation at the midbrain-hindbrain organiser. Development. 2011 Sep;138(17):3745-57. Epub 2011 Jul 27.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 9

stage 10

stage 12

Foregut
Isthmus/MHB
Midbrain
Rhombomeres

24 hours after electroporation
Fgf8 (blue) and Krox20 (red)
GFP control embryo
double in situ: Fgf8 (red) and Hoxa2 (blue)
prosencephalon

Entry 44

GEISHA Id	FGF8.Bothe.2011	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Bothe I, Tenin G, Oseni A, Dietrich S., Dynamic control of head mesoderm patterning., Development. 2011 Jul;138(13):2807-21.
Copyright	Permission from the authors and the Company of Biologists Ltd. Copyright held by the authors.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

Hensen's Node
Primitive Streak

7-12

stage 8

stage 9

Early Endoderm
Endoderm
Forebrain
Isthmus/MHB

stage 13

Eye
Forebrain
Isthmus/MHB
Nasal Placode/Nerve
Pharyngeal Arches and Clefts
Surface Ectoderm

Entry 45

GEISHA Id	FGF8.Tossell.2011B	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Tossell K, Andreae LC, Cudmore C, Lang E, Muthukrishnan U, Lumsden A, Gilthorpe JD, Irving C. Lrrn1 is required for formation of the midbrain-hindbrain boundary and organiser through regulation of affinity differences between midbrain and hindbrain cells in chick. Dev Biol. 2011 Apr 15;352(2):341-52.
Copyright	Copyright © 2011 Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

no images

Entry 46

GEISHA Id	FGF8.SzaboRogers.2009	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Szabo-Rogers HL, Geetha-Loganathan P, Whiting CJ, Nimmagadda S, Fu K, Richman JM. Novel skeletogenic patterning roles for the olfactory pit. Development. 2009 Jan;136(2):219-29. Epub 2008 Dec 4.
Copyright	Published by The Company of Biologists 2009. This material is protected by a copyright retained by the authors. Permission requested from Dr. Richman.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (Acc.#NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-15

stage 15

Entry 47

GEISHA Id	FGF8.Tomas.2011	Data Source	Publication
Complete cDNA Template Probe	show CTCAGCCGGCGGCTTGTGCGCACCTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGA AAATCAATGCGATGGCCGAGGACGGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCG CATCAAAGGGGCGGCCACGGGGTTCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGC AAGGACTGCGTCTTCACCGAGATCGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACA TGGCCTTCACCCGCAAGGGCCGCCCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCG GCTCCCCAAGGGCCACCAGACCACCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAA AGGACTCGAAACTCCAGCGCCAGGGTGCGCCCATGACGCGTGCCCCTCGGTGGACTGACGTAGAGACATTGTGATAGGG TTACTAAAGGAAAAAAAAAACCCAACGGGGGATGGGGGGAGGATACGAGAACTACATGCTCTAGNTGTGGTAATGTGGG GGTAGTCTGTTTTTACAAAAAAAAAAACCAAACAGACAAAAA
Citation	Tomás AR, Certal AC, Rodríguez-León J. FLRT3 as a key player on chick limb development. Dev Biol. 2011 Jul 15;355(2):324-33.
Comments	Probe for fgf8 was obtained from chicken ChEST320b9.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-14

stage 14

Limb Buds
Wing AER

control embryo

Entry 48

GEISHA Id	FGF8.Grevellec.2011	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Grevellec A, Graham A, Tucker AS. Shh signalling restricts the expression of Gcm2 and controls the position of the developing parathyroids. Dev Biol. 2011 May 15;353(2):194-205. Epub 2011 Feb 22.
Copyright	Copyright © 2011 Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Pharyngeal Arches and Clefts

Entry 49

GEISHA Id	FGF8.Abello.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Abelló G, Khatri S, Radosevic M, Scotting PJ, Giráldez F, Alsina B., Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode. Dev Biol. 2010 Mar 1;339(1):166-78. Epub 2010 Jan 4.
Copyright	Copyright © 2009 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_001012767.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-9

stage 9

Foregut
Neural Plate/Tube

Entry 50

GEISHA Id	FGF8.Lorente-Cánovas.2012	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Lorente-Cánovas B, Marín F, Corral-San-Miguel R, Hidalgo-Sánchez M, Ferrán JL, Puelles L, Aroca P. Multiple origins, migratory paths and molecular profiles of cells populating the avian interpeduncular nucleus. Dev Biol. 2012 Jan 1;361(1):12-26.
Copyright	Copyright © 2011 Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-26

stage 26

FGF8 and HOXA2 (blue)
FGF8, HOXA2, and DBH in situ hybridization (blue)
PAX7 antibody (brown)

Entry 51

GEISHA Id	FGF8.Gros.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Gros J, Feistel K, Viebahn C, Blum M, Tabin CJ. Cell movements at Hensens node establish Left/Right asymmetric gene expression in the chick. Science. 2009 May 15; 324(5929): 941944.
Copyright	© 2012 American Association for the Advancement of Science. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

4-5

stage 4

stage 5

Anterior Neuropore
Isthmus/MHB

Entry 52

GEISHA Id	FGF8.Adams.2000	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Adams KA, Maida JM, Golden JA, Riddle RD. The transcription factor Lmx1b maintains Wnt1 expression within the isthmic organizer. Development. 2000 May;127(9):1857-67.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

13-15

stage 15

Ear/Otic Placode
Forebrain
Isthmus/MHB
Pharyngeal Arches and Clefts

Entry 53

GEISHA Id	FGF8.Farrell.2001	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Farrell MJ, Burch JL, Wallis K, Rowley L, Kumiski D, Stadt H, Godt RE, Creazzo TL, Kirby ML. FGF-8 in the ventral pharynx alters development of myocardial calcium transients after neural crest ablation. J Clin Invest. 2001 June 15; 107(12): 15091517.
Copyright	Copyright © 2001, American Society for Clinical Investigation
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 14

stage 18

Entry 54

GEISHA Id	FGF8.Darnell.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Darnell DK, Stanislaw S, Kaur S, Antin PB. Whole mount in situ hybridization detection of mRNAs using short LNA containing DNA oligonucleotide probes. RNA. 2010 March; 16(3): 632637.
Copyright	Copyright © 2010 RNA Society
Comments	Probes were multiple LNAs whose sequences can be found at http://rnajournal.cshlp.org/content/suppl/2010/01/08/rna.1775610.DC1/Supp_figs_S1_S3.pdf. NCBI (acc #NM_001012767.1) is given for reference only.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 11

Anterior Neuropore
Rhombomeres
Tail

13-18

stage 14

Forebrain
Isthmus/MHB
Tail

19-23

stage 20

stage 23

Limb Buds
Nasal Placode/Nerve
Pharyngeal Arches and Clefts
Wing AER

Entry 55

GEISHA Id	FGF8.Fisher.2008	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Fisher ME, Clelland AK, Bain A, Baldock RA, Murphy P, Downie H, Tickle C, Davidson DR, Buckland RA. Integrating technologies for comparing 3D gene expression domains in the developing chick limb. Dev Biol. 2008 May 1;317(1):13-23. Epub 2008 Feb 7.
Copyright	Copyright © 2008 Elsevier Inc.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 22

stage 23

stage 24

Leg AER
Limb Buds
Placode
Wing AER

double label

Entry 56

GEISHA Id	FGF8.Sabado.2012	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Sabado V, Barraud P, Baker CV, Streit A. Specification of GnRH-1 neurons by antagonistic FGF and retinoic acid signaling. Dev Biol. 2012 Feb 15;362(2):254-62.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 15

stage 17

stage 18

Eye
Face Mesenchyme
Midbrain
Nasal Placode/Nerve

Entry 57

GEISHA Id	FGF8.Altabef.2002	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Altabef M, Tickle C. Initiation of dorso-ventral axis during chick limb development. Mech Dev. 2002 Aug;116(1-2):19-27.
Copyright	Copyright © 2012 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767.1 ). The probe for FGF8 was a gift.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-17

stage 16

stage 17

Leg AER
Myotome
Wing AER
Wing Muscles

Entry 58

GEISHA Id	FGF8.Bertrand.2000	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Bertrand N, Médevielle F, Pituello F FGF signalling controls the timing of Pax6 activation in the neural tube. Development. 2000 Nov;127(22):4837-43.
Copyright	copyright held by the authors. Published by the Company of Biologists, Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_001012767.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

Paraxial Mesoderm
Tail

Entry 59

GEISHA Id	FGF8.Sanchez-Arrones.2009	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Sánchez-Arrones L, Ferrán JL, Rodríguez-Gallardo L, Puelles L. Incipient forebrain boundaries traced by differential gene expression and fate mapping in the chick neural plate. Dev Biol. 2009 Nov 1;335(1):43-65. Epub 2009 Aug 19.
Copyright	Copyright © 2009 Elsevier Inc. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_001012767.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-8

stage 8

Anterior Intestinal Portal
Gut
Neural Plate/Tube

Entry 60

GEISHA Id	FGF8.Masuda.2012	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Masuda T, Sakuma C, Taniguchi M, Kanemoto A, Yoshizawa M, Satomi K, Development of the dorsal ramus of the spinal nerve in the chick embryo: a close relationship between development and expression of guidance cues. Brain Res. 2012 Oct 22;1480:30-40. .
Copyright	Copyright © 2012 Elsevier B.V. All rights reserved
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 20

stage 22

Myotome

In situ: FGF8 (blue)
In situ: FGF8(blue)
antibody: MF20 (brown)
antibody: Islet1/2 (brown)
antibody: MF20(brown)

25-29

stage 26

stage 29

Myotome
Unlabeled

In situ: FGF8 (blue)
antibody: MF20 (brown)
antibody: Islet1/2 (brown)
antibody: MF20 (brown)

Entry 61

GEISHA Id	FGF8.Wade.2012	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Wade C, Brinas I, Welfare M, Wicking C, Farlie PG. Twist2 contributes to termination of limb bud outgrowth and patterning through direct regulation of Grem1. Dev Biol. 2012 Oct 1;370(1):145-53.
Copyright	Copyright © 2012 Elsevier Inc. All rights reserved
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767.1).

Stage

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19-24

stage 22

Leg AER

Left hand side is control hindlimb - not infected
Right hand side - 48 hr after infection
Right hand side - 72 hr after infection

stage 25

Leg

Left side: control hindlimb

Entry 62

GEISHA Id	FGF8.Kumar.2012B	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Kumar M, Ray P, Chapman SC. Fibroblast growth factor and bone morphogenetic protein signaling are required for specifying prechondrogenic identity in neural crest-derived mesenchyme and initiating the chondrogenic program. Dev Dyn. 2012 Jun;241(6):1091-103
Copyright	Copyright © 2012 Wiley Periodicals, Inc.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001012767.1).

Stage

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stage 13

Endoderm
Pharyngeal Arches and Clefts
Surface Ectoderm

Entry 63

GEISHA Id	FGF8.Tsikolia.2012	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Comments	According to the authors, "Digoxigenin-labeled mRNA probes for nodal, shh, fgf8 were produced using the plasmid DNAs previously published ( Boettger et al., 1999, Essner et al., 2002 and Levin et al., 1995)" As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence was obtained from NCBI (acc# NM_001012767.1).

Stage

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4-5

stage 4

stage 5

Epiblast
Lateral Plate Mesoderm
Primitive Streak

HH4+ stage embryo
HH5+ stage embryo
HH5- stage embryo

Entry 64

GEISHA Id	FGF8.Tenin.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Tenin G, Wright D, Ferjentsik Z, Bone R, McGrew MJ, Maroto M. The chick somitogenesis oscillator is arrested before all paraxial mesoderm is segmented into somites. BMC Dev Biol. 2010 Feb 25;10:24.
Copyright	authors retain copyright of their article, and grant any third party the right to use reproduce and disseminate the article, subject to the terms of BMC Developmental Biology copyright and license agreement
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_001012767.1

Stage

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13-16

stage 16

Tail

guessed on stage

Entry 65

GEISHA Id	FGF8.Sánchez-Guardado.2009	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Sánchez-Guardado LO, Ferran JL, Mijares J, Puelles L, Rodríguez-Gallardo L, Hidalgo-Sánchez M. Raldh3 gene expression pattern in the developing chicken inner ear. J Comp Neurol. 2009 May 1;514(1):49-65. doi: 10.1002/cne.21984.
Copyright	Copyright © 19992013 John Wiley & Sons, Inc. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_001012767.1

Stage

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19-20

stage 20

Ear/Otic Placode

Entry 66

GEISHA Id	FGF8.Hirashima.2008	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Hirashima M, Kobayashi T, Uchikawa M, Kondoh H, Araki M. Anteroventrally localized activity in the optic vesicle plays a crucial role in the optic development. Dev Biol. 2008 May 15;317(2):620-31. doi: 10.1016/j.ydbio.2008.03.010. Epub 2008 Mar 20.
Copyright	Copyright © 2008 Elsevier Inc. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_001012767.1

Stage

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Location (click to highlight)

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7-11

stage 11

Eye
Forebrain
Isthmus/MHB
Pharyngeal Arches and Clefts

Entry 67

GEISHA Id	FGF8.Marcucio.2005	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Marcucio RS, Cordero DR, Hu D, Helms JA. Molecular interactions coordinating the development of the forebrain and face. Dev Biol. 2005 Aug 1;284(1):48-61.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence was obtained from NCBI (NM_001012767.1).

Stage

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Location (click to highlight)

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19-20

stage 20

Nasal Placode/Nerve

frontalnasal ectoderm

all

Entry 68

GEISHA Id	FGF8.Creuzet.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Creuzet S, Schuler B, Couly G, Le Douarin NM. Reciprocal relationships between Fgf8 and neural crest cells in facial and forebrain development. Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4843-7.
Copyright	Copyright © 2004, The National Academy of Sciences
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_001305272.1).

Stage

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Location (click to highlight)

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13-18

stage 15

prosencephalon neuroepithelium

stage 31

Entry 69

GEISHA Id	Fgf8.Abzhanov.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Abzhanov A, Tabin CJ. Shh and Fgf8 act synergistically to drive cartilage outgrowth during cranial development. Dev Biol 2004 Sep 1;273(1):134-48.
Copyright	Copyright © 2004 Elsevier Inc.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the complete sequence was obtained from NCBI (acc # NM_001012767).

Stage

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Location (click to highlight)

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31-36

stage 36

Unlabeled

No Fgf8 expression in epithelium or mesenchyme of the beak.

Entry 70

GEISHA Id	Fgf8.Abzhanov.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Abzhanov, A., Cordero, D. R., Sen, J., Tabin, C. J. and Helms, J. A. Cross–regulatory interactions between Fgf8 and Shh in the avian frontonasal prominence. Congenit Anom (Kyoto). 2007 Dec;47(4):136-48.
Copyright	Copyright © 2007 The Authors, Journal compilation © 2007 Japanese Teratology Society
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence above was obtained from NCBI (acc # NM_001012767).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 15

Pharynx
Surface Ectoderm

dorsal neuroectoderm, dorsal facial ectoderm, pharyngeal endoderm
facial ectoderm

19-24

stage 20

stage 22

Surface Ectoderm
Telencephalon

dorsal facial ectoderm, dorsal telencephalon, optic recess.
nasal pits, maxillae, mandibles, hyoid arches
optic recess
surface of the head, maxillae, mandibles, hyoid arches

stage 25

Nasal Placode/Nerve
Pharynx
Telencephalon

maxilla
optical recess

all

Entry 71

GEISHA Id	FGF8.Yuan.1999B	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Yuan S, Schoenwolf GC. Reconstitution of the organizer is both sufficient and required to re-establish a fully patterned body plan in avian embryos. Development. 1999 Jun;126(11):2461-73
Copyright	Great Britain © The Company of Biologists Limited 1999
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

Ectoderm
Notochord

neuroectroderm

31-44

stage 44

Entry 72

GEISHA Id	Fgf8.Jungbluth.2001	Data Source	Publication
Complete cDNA Template Probe	show ATGGACCCCTGCTCCTCGCTCTTCAGCTACGTGTTCATGCACTTGTTCGTCCTCTGCCTGCAAGCCCAGGTAACTGTTC AGTCCCCACCTAATTTTACACAGCATGTGAGGGAGCAGAGCCTGGTGACAGATCAGCTCAGCCGGCGGCTCGTGCGCAC CTACCAGCTGTACAGCCGGACCAGCGGGAAGCACGTGCAGATCTTGGACAACAAGAAAATCAATGCGATGGCCGAGGAC GGGGATGTGCACGCCAAGCTCATCGTCGAGACCGACACCTTTGGGAGCCGCGTGCGCATCAAAGGGGCGGCCACGGGGT TCTACATCTGCATGAACAAGAAGGGGAAACTGATCGGCAAGAGTAACGGCAAAGGCAAGGACTGCGTCTTCACTGAGAT CGTGCTGGAGAACAACTACACGGCGCTGCAGAACGCCAAGTACGAGGGCTGGTACATGGCCTTCACCCGCAAGGGCCGC CCGCGCAAGGGCTCCAAGACCCGGCAGCACCAACGCGAGGTGCACTTCATGAAGCGGCTCCCCAAGGGCCACCAGACCA CCGAGCCCCACCGGCGCTTCGAGTTCCTCAACTACCCCTTCAACCGCAGGAGCAAAAGGACTCGAAACTCCAGCGCCTC CCTGCGCCCATGA
Citation	Stefan Jungbluth, Chris Phelps, Andrew Lumsden. CEPU-1 expression in the early embryonic chick brain. Mech Dev. 2001 Mar;101(1-2):195-7.
Copyright	Copyright © 2001 Elsevier Science Ireland Ltd.
Comments	Authors indicated that Fgf8 probe was a gift of I. Mason. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence above was obtained from NCBI (accession NM_001012767.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12