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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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GBX2
gastrulation brain homeobox 2

Gene

Official Symbol	GBX2
Official Full Name	gastrulation brain homeobox 2
CGNC ID	10048
also known as	homeobox protein GBX-2\|gastrulation and brain-specific homeobox protein 2\|gastrulation brain homeo box 2\|gastrulation brain homeobox 2 protein
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_205068
Polypeptide	NP_990399
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.565

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	2637	ENSG00000168505		MIM:601135
Mouse	14472		All phenotypic alleles (9):Targeted, knock-out(1) Targeted, other(8)	MGI:95668
Xenopus	100485559, 399181			855770
Zebrafish	245948	ENSDARG00000002933	ZDB-GENE-020509-2	ZFIN:ZDB-GENE-020509-2

Gene Ontology

Molecular Function	sequence-specific DNA binding, sequence-specific DNA binding transcription factor activity
Biological Process	transcription, DNA-templated
Cellular Component	nucleus

Links to other databases

Entrez Gene	395950
Ensembl Gene
KEGG	gga:395950

Entry 1

GEISHA Id	GBX2.Vieira.2005	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Vieira C, Garda AL, Shimamura K, Martinez S. Thalamic development induced by Shh in the chick embryo. Dev Biol. 2005 Aug 15;284(2):351-63.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205068).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-23

stage 23

Central Nervous System

Thalamus

Entry 2

GEISHA Id	GBX2.Bok.2005	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Bok J, Bronner-Fraser M, Wu DK. Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear. Development. 2005 May;132(9):2115-24. Epub 2005 Mar 23.
Copyright	Copyright for articles in Development are retained by the authors. Permission granted by MBF.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205068).

no images

Entry 3

GEISHA Id	GBX2.Garcia-Lopez.2009	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	García-López R, Soula C, Martínez S. Expression analysis of Sulf1 in the chick forebrain at early and late stages of development. Dev Dyn. 2009 Sep;238(9):2418-29.
Copyright	Copyright © 2009 John Wiley & Sons, Inc. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205068.1).

Stage

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Location (click to highlight)

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31-40

stage 40

Diencephalon

double label

Entry 4

GEISHA Id	GBX2.Sanchez-Calderon.2004	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Sánchez-Calderón H, Martín-Partido G, Hidalgo-Sánchez M. Otx2, Gbx2, and Fgf8 expression patterns in the chick developing inner ear and their possible roles in otic specification and early innervation. Gene Expr Patterns. 2004 Oct;4(6):659-69.
Copyright	Reprinted with permission from Elsevier. Copyright © 2004 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_205068).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Ear/Otic Placode

double labeled

25-30

stage 27

Ear/Otic Placode

double labeled

31-34

stage 34

Ear/Otic Placode

double labeled

Entry 5

GEISHA Id	GBX2.Paxton.2010	Data Source	Publication
Complete cDNA Template Probe	show GGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAGGCGGGCAACGCCAGCTCCAAGGCG GGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCCGCTTCGCCATCAGGAGTCAGCACC AGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACAACCGGGGTTTTCGCCGGACCCGCT CTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAGCGAACGACCCAAACGAACGCCTCC CCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGCGATACGGCGGAGCCCCACGCCGGC AGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGGACCGCATCGCCCCGCAGCGCTACG GGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGTTGAGAGGAGAGGGGGAGAGAGAGA GAGAGGAGGGGAGGGGGGGGAGGAGGACGGACGGACGGACAGACGGACACCGAGGAAAGCACGAATTCAGCGTTGTGGT TTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAATTTAAGGAGCCAACTCCCGTCTGTGAAT
Citation	Paxton CN, Bleyl SB, Chapman SC, Schoenwolf GC. Identification of differentially expressed genes in early inner ear development. Gene Expr Patterns. 2010 Jan;10(1):31-43. Epub 2009 Nov 11.
Copyright	2009 Elsevier B.V. All rights reserved.
Comments	The cDNA template used to generate the probe was obtained by PCR as described 5-ggtgcaggtgaagatctggt-3; 5-attcacaagacgggagttgg −3

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

Early Mesoderm
Epiblast
Head Process
Primitive Streak

7-11

stage 7

stage 9

stage 11

Ear/Otic Placode
Early Mesoderm
Heart Tube
Hindbrain
Isthmus/MHB
Midbrain
Neural Plate/Tube
Splanchnic Mesoderm

Entry 6

GEISHA Id	GBX2.Merchan.2011	Data Source	Publication
Complete cDNA Template Probe	show AGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTGG AGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGTT CACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGATC GCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAGG CGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCCG CTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACAA CCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAGC GAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGCG ATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGGA CCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGTT GAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGACG GACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAATT TAAGGAGCCAACTCCCGTCTTGTGAAT
Citation	Merchán,P, Bardet, SM, Puelles, L, Ferran, JL, Comparison of Pretectal Genoarchitectonic Pattern between Quail and Chicken Embryos. Front Neuroanat. 2011; 5: 23. Prepublished online 2010 December 29. Published online 2011 April 5
Copyright	Copyright © 2011 Merchán, Bardet, Puelles and Ferran.
Comments	The probe was amplified by PCR using the following primers: 5′-AGCGACCTCGACTACAGCTC-3' and 5′-ATTCACAAGACGGGAGTTGG-3′and was extracted form NCBI acc. # NM_205068.1.

Stage

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Location (click to highlight)

Comments (click to highlight)

25-27

stage 26

stage 27

Diencephalon
Mesencephalon
Unlabeled Embryonic

PcP precommissural pretectum, JcP juxtacommissural pretectum, CoP commissural pretectum, Th Thalamus , mes mesencephalon, Hab habenular region
PcP precommissural pretectum, JcP juxtacommissural pretectum, CoP commissural pretectum, Th Thalamus, mes mesencephalon, Hab habenular region
Quail

Entry 7

GEISHA Id	GBX2.Tossell.2011	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Tossell K, Kiecker C, Wizenmann A, Lang E, Irving C. Notch signalling stabilises boundary formation at the midbrain-hindbrain organiser. Development. 2011 Sep;138(17):3745-57. Epub 2011 Jul 27.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205068).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-10

stage 9

stage 10

Isthmus/MHB
Rhombomeres

24 hours after electroporation
double in situ: OTX2(red) and GBX2(blue)
double in situ: Otx2 (red) and Gbx2 (blue)

Entry 8

GEISHA Id	GBX2.Tossell.2011B	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Tossell K, Andreae LC, Cudmore C, Lang E, Muthukrishnan U, Lumsden A, Gilthorpe JD, Irving C. Lrrn1 is required for formation of the midbrain-hindbrain boundary and organiser through regulation of affinity differences between midbrain and hindbrain cells in chick. Dev Biol. 2011 Apr 15;352(2):341-52.
Copyright	Copyright © 2011 Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205068).

no images

Entry 9

GEISHA Id	GBX2.Larsen.2001	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Larsen CW, Zeltser LM, Lumsden A. Boundary formation and compartition in the avian diencephalon. J Neurosci. 2001 Jul 1;21(13):4699-711.
Copyright	Copyright © 2012 by the Society for Neuroscience
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205068.1).

no images

Entry 10

GEISHA Id	GBX2.Sánchez-Guardado.2009	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Sánchez-Guardado LO, Ferran JL, Mijares J, Puelles L, Rodríguez-Gallardo L, Hidalgo-Sánchez M. Raldh3 gene expression pattern in the developing chicken inner ear. J Comp Neurol. 2009 May 1;514(1):49-65. doi: 10.1002/cne.21984.
Copyright	Copyright © 19992013 John Wiley & Sons, Inc. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_205068.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-20

stage 20

Ear/Otic Placode

Entry 11

GEISHA Id	Gbx2.Jungbluth.2001	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Stefan Jungbluth, Chris Phelps, Andrew Lumsden. CEPU-1 expression in the early embryonic chick brain. Mech Dev. 2001 Mar;101(1-2):195-7.
Copyright	Copyright © 2001 Elsevier Science Ireland Ltd.
Comments	Authors indicated that Gbx2 probe was a gift of A. Leutz. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence above was obtained from NCBI (accession NM_205068.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-11

stage 11

Isthmus/MHB

double in situ: Gbx2 in red, CEPU-1 in blue.

all

Entry 12

GEISHA Id	Gbx2.Hidalgo-Sánchez.1999	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Hidalgo-Sánchez M, Millet S, Simeone A, Alvarado-Mallart RM. Comparative analysis of Otx2, Gbx2, Pax2, Fgf8 and Wnt1 gene expressions during the formation of the chick midbrain/hindbrain domain. Mech Dev 1999 Mar;81(1-2):175-8.
Copyright	Copyright © 1999 Elsevier Science Ireland Ltd.
Comments	Authors indicated the probe was synthesized from the full-length chicken GBX2 cDNA. Above sequence is obtained from NCBI Accession number NM_205068.1.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

Isthmus/MHB
Mesencephalon
Rhombomeres

Double ISH, Gbx-2 in purple.

19-20

stage 20

Isthmus/MHB
Rhombomeres

Double ISH, Gbx-2 in purple.
Double ISH, Gbx-2 in purple.

Entry 13

GEISHA Id	GBX2.Freter.2012	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGGGGAGGAGGAAGAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGC TGATGATGATGCAGCGCCCGCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGC CCCCGGCCACTTCGTCTACACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCC CTGCCGCAGGCCGCCCTGCAGCCGCCGCTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCC CCGGGCTGGCGCAGGGCATGGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCG CCCCGAGGCCGCCAGGAAGTTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGC GGCGGCGGAGACGACGGCAAAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCG GGGCCCTCCGCGGCGGCCCCAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGA CAGCGACCTCGACTACAGCTCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTG GAGGAGAACCCCCCCAGCGCCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGT TCACCAGCGAGCAGCTGCTGGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGAT CGCGCACGCCCTGAAGCTGAGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAG GCGGGCAACGCCAGCTCCAAGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCC GCTTCGCCATCAGGAGTCAGCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACA ACCGGGGTTTTCGCCGGACCCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAG CGAACGACCCAAACGAACGCCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGC GATACGGCGGAGCCCCACGCCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGG ACCGCATCGCCCCGCAGCGCCACGGGGTAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGT TGAGAGGAGAGGGGGAGAGAGAGAGAGAGAGGAGGGGAGGGGGGGGGGGGGAGGAGGACGGACGGACGGACGGACAGAC GGACACCGAGGAAAGCACGAATTCAGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAAT TTAAGGAGCCAACTCCCGTCTTGTGAATAAAAGCCGACAAAGAAACCCGGAATTC
Citation	Freter S, Muta Y, O'Neill P, Vassilev VS, Kuraku S, Ladher RK. Pax2 modulates proliferation during specification of the otic and epibranchial placodes. Dev Dyn. 2012 Nov;241(11):1716-28
Copyright	Copyright © 2012 Wiley Periodicals, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence was obtained from NCBI (NM_205068.1). GBX2 is also known as: GBX1 in NCBI.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-14

stage 14

Central Nervous System
Ear/Otic Placode
Placode

control = left side of embryo
cross section of figure 4M.
stage 13/14, 16 to 22 somites

all

Entry 14

GEISHA Id	GBX2.Trevers.2023	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show AAAATGTGAATGGGCGCGGGGGAGCGGGCGGGCAGAGGCAGCGCGGTGCGGGGCGGCGGGGGGCGGCCGGCCCCGATGT GCGGGCGGCGGCGGCCCCGCTCCGCCCCGGCTCGGCCCCACGCGTGGGCGCTGCGCGGCGCTGAGCTGCGGGAGCCGGA GCCCGAGCCCGAGCAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAGGAA GAAGGTGGTCGCCGCTGCTCCTCCGCCGGCCCGGCATGAGCGCGGCTTTCCAGCCCTCGCTGATGATGATGCAGCGCCC GCTGGGAAGCAGCACGGCCTTCAGCATCGATTCGCTCATCGGCAGCCCCCCGCCGCCCGCCCCCGGCCACTTCGTCTAC ACCGGCTACCCCATGTTCATGCCGTACCGGCCCGTCGTGCTGCCCCCCCCGCCGCCCGCCCTGCCGCAGGCCGCCCTGC AGCCGCCGTTGCCCCCCGCGCCGCCCCCGCCGCTGCCCGCGTTGCCCGGAGCCTTCTGCCCCGGGCTGGCGCAGGGCAT GGCCCTCACCTCCACGCTGATGGCCGCGCTGCCCGGCTCCTTCCCCGCCTCCCCGCCGCGCCCCGAGGCCGCCAGGAAG TTCGCCCCCCCGGGGAACTTCGACAAGGCCGACGGACTGCCTCCCCCGGACGGCGGCGGCGGCGGCGGAGACGACGGCA AAACCGGGGGGGGGCTGCTACCCTTCCCCGCTGCCGACGCCGTGCACGCATCGCTGGCCGGGGCCCTCCGCGGCGGCCC CAAAGACGATCCCAAAGCGGAGGAGGAGGCGAAGGGCCGCGAGGAGAACTTCTCCATGGACAGCGACCTCGACTACAGC TCCGACGAGAACGGCCCCGCGCCGGCGGCCCCGCGGGAGGAGGACTGTGGCACCGCGTTGGAGGAGAACCCCCCCAGCG CCGCCAACGCCGCCGCCAACGCCGCGGCCACGGGGAAGAACCGACGGCGGCGGACGGCGTTCACCAGCGAGCAGCTGCT GGAGCTGGAGAAGGAGTTCCACTGCAAGAAGTACCTCTCGCTGACGGAGCGCTCGCAGATCGCGCACGCCCTGAAGCTG AGCGAGGTGCAGGTGAAGATCTGGTTCCAGAACAGGCGAGCCAAATGGAAGCGGGTGAAGGCGGGCAACGCCAGCTCCA AGGCGGGGGAACCGTCGCGGAACCCCAAGATCGTCGTGCCCATCCCCGTGCACGTCAGCCGCTTCGCCATCAGGAGTCA GCACCAGCAGCTGGAGCAGGCGCGGCCCTGAGCGCCGCTGGGGGGGCAAAAAAGTTGACAACCGGGGTTTTCGCCGGAC CCGCTCTGAAAAGCGAAACGAGCGACGGAAACCAACCCAAACAGACGAAAACGACCCAAGCGAACGACCCAAACGAACG CCTCCCCGCCCCCCCAAAATGAACCTAGAAGCGACGCGACTCCACCGGCGACCCACGAGCGATACGGCGGAGCCCCACG CCGGCAGCGAGCGGCAGAACTGAAGGCGGAACGCCGCGCGGAGCGGGGCCGGGGACCGGGACCGCATCGCCCCGCAGCG CCACGGGATAACGGCGCTGGGGGAGCCGTCGGTGCGGGGCCGCCCCTCGCAGAGCCGCGTTGAGAGGAGAGGGGGAGAG AGAGAGAGAGAGGAGGGGAGGGGGGGGAGGAGGACGGACGGACGGACGGACAGACGGACACCGAGGAAAGCACGAATTC AGCGTTGTGGTTTAATTTATTTCGATGTAGCCCGTTTCCTTCTAAGTTATGAAATTTAAGGAGCCAACTCCCGTCTTGT GAATAAAAGCCGACAAAGAAAC
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205068.2 ).