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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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GDNF
glial cell derived neurotrophic factor

The human orthologue of this gene is associated
with the following human diseases:

Gene

Official Symbol	GDNF
Official Full Name	glial cell derived neurotrophic factor
CGNC ID	2717
also known as	glial cell line-derived neurotrophic factor\|glial cell line-derived neurotrophic factor long form, GDNF, Glial cell line-derived neurotrophic factor short form
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC

Sequence Information

Genomic
RNA	XM_425018
Polypeptide	XP_425018

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	2668	ENSG00000168621	MIM:209880	MIM:600837
Mouse	14573	ENSMUSG00000022144	All phenotypic alleles (7):Targeted, knock-out(2) Targeted, other(5)	MGI:107430
Xenopus	100498061, 779087, 100337571			483794
Zebrafish	79379	ENSDARG00000039959	ZDB-GENE-010226-1, ZDB-GENE-110609-2	ZFIN:ZDB-GENE-010226-1, ZFIN:ZDB-GENE-110609-2

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	395569
Ensembl Gene
KEGG	gga:395569

Entry 1

GEISHA Id	GDNF.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATCCAGCAAAAAGGGAAGGAGG
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGAGGACAGGTCATCGTCAAAGGC	cDNA Template Length	261
Forward cDNA Template Probe	show `AAACAAAAGGGCAAAATCGGGGATGTGTCTTAACAGAAATACATTTAAATGTGACTGACTTGGATTTGGGATACGAAAC CAAAGAAGAGCTCATCTTCCGGTATTGCAGTGGATCTTGTGATGCAGTTGAGACCACGTATGACAAAATTTTGAAAAAC TTAACCAGAAAGAAAAAACTAGTCAATGACAAAGTGAGGCAAGCTTGCTGCAGACCCACAGCCTTTGACGATGACCTGT CCTCCTTTAGTGAGGGTTAATTTCTAGAGCAAA`
Reverse cDNA Template Probe	show `AAAATGAAGAAAAAAAATTGTGGGGTCTGCAGCAAGCTTGCCTCACTTTGTCATTGACTAGTTTTTTCTTTCTGGTTAA GTTTTTCAAAATTTTGTCATACGTGGTCTCAACTGCATCACAAGATCCACTGCAATACCGGAAGATGAGCTCTTCTTTG GTTTCGTATCCCAAATCCAAGTCAGTCACATTTAAATGTATTTCTGTTAAGACACATCCCCGATTTTTGCCCTTTTGGT TCCTCCTTCCCTTTTTGCTGGATATTCTGCGGCCGCAAGCTTTTATAAAA`

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 13

Intermediate Mesoderm

Staining observed in neural tube may be due to trapping.

Entry 2

GEISHA Id	GDNF.Nagy.2006	Data Source	Publication
Complete cDNA Template Probe	show `TGCCAGAGGATTACCCAGATCAGTTCGATGAGGTAGTGGACTTTATTCAAGCCACTATTAAGAGACTGAGGAGATCTCC AGATAAACAGACTCCGATTTTTTCTAGGCGGGAGAGGAACCGGCAAAGTGCAGCCACAAACGTAGAGAATTCCAGCAAA AAGGGAGGAGGAACCAAAAGGGCAAAAATCGGGGATGTGTCTTAACAGAAATACATTTAAATGTGACTGACTTGGATTT GGGATAGAAACCAAAGAAGAGCTCATCTTCCGGTATTGCAGTGGATCTTGTGATGCAGTTGAGACCACGTATGACAAAA TTTTGAAAAACTTAACCAGAAAGAAAAAACTAGTCAATGACAAAGTGAGGCAAGCTTGCTGCAGACCCACAGCCTTTGA CGATGACCT`
Citation	Nagy N, Goldstein AM. Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric nervous system. Dev Biol. 2006 May 1;293(1):203-17. Epub 2006 Mar 6.
Copyright	"Reprinted from Developmental Biology, 293, Nagy N, Goldstein AM, Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric nervous system., 203-17, Copyright (2006), with permission from Elsevier". Copyright © (2006) Elsevier B.V. All rights reserved.
Comments	According to the authors: Antisense GDNF RNA probe was generated by amplifying a fragment of GDNF sequence from total RNA prepared from E9 chick gut using Trizol Reagent (Invitrogen). The following oligonucleotide primers were used: upstream primer, 5′-tgccagaggattacccagat-3′; downstream primer, 5′-aggtcatcgtcaaaggctgt-3′.

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Renal

nephrogenic mesenchyme

25-30

stage 26

stage 30

Hindgut
Stomach

cloaca and cecal primordia
mesenchymal cells
proventriculus
throughout the intestine

31-35

stage 35

Hindgut

throughout the intestine

Entry 3

GEISHA Id	GDNF.Homma.2000	Data Source	Publication
Complete cDNA Template Probe	show ATGTTACAAAGCATTTTTTCTCTTCTTCAAACAGCTAATATGCCAGAGGATTACCCAGATCAGTTCGATGAGGTAGTGG ACTTTATTCAAGCCACTATTAAGAGACTGAGGAGATCTCCAGATAAACAGACTCCGATTTTTTCTAGGCGGGAGAGGAA CCGGCAAAGTGCAGCCACAAACGTAGAGAATTCCAGCAAAAAGGGAAGGAGGAACCAAAAGGGCAAAAATCGGGGATGT GTCTTAACAGAAATACATTTAAATGTGACTGACTTGGATTTGGGATACGAAACCAAAGAAGAGCTCATCTTCCGGTATT GCAGTGGATCTTGTGATGCAGTTGAGACCACGTATGACAAAATTTTGAAAAACTTAACCAGAAAGAAAAAACTAGTCAA TGACAAAGTGAGGCAAGCTTGCTGCAGACCCACAGCCTTTGACGATGACCTGTCCTTTTTGGATGATAACCTGGTTTAC CACATACTGAAAAAACATTCCGCAAAAAGGTGTGGATGTGTCTGA
Citation	Homma S, Oppenheim RW, Yaginuma H, Kimura S. Expression pattern of GDNF, c-ret, and GFRalphas suggests novel roles for GDNF ligands during early organogenesis in the chick embryo. Dev Biol. 2000 Jan 1;217(1):121-37.
Copyright	Reprinted with permission from Elsevier. Copyright © 2000 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (XM_425018).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 9

Intermediate Mesoderm
Pharyngeal Arches and Clefts
Pharynx

Pronephrogenic IM

13-18

stage 13

stage 18

Allantois
Cranial Ganglion
Foregut
Head Mesenchyme
Hindgut
Intermediate Mesoderm
Neural Plate/Tube
Pharyngeal Arches and Clefts
Pharynx
Sinus Venosus
Veins

Distal Ganglia of the Facial (VII) and Glossopharyngeal (IX) Nerves
Epithelial Lining of the Pharyngeal Cleft
Floor Plate
Nephrogenic Cord
Primary Head Veins

Entry 4

GEISHA Id	GDNF.Burns.2005	Data Source	Publication
Complete cDNA Template Probe	show ATGTTACAAAGCATTTTTTCTCTTCTTCAAACAGCTAATATGCCAGAGGATTACCCAGATCAGTTCGATGAGGTAGTGG ACTTTATTCAAGCCACTATTAAGAGACTGAGGAGATCTCCAGATAAACAGACTCCGATTTTTTCTAGGCGGGAGAGGAA CCGGCAAAGTGCAGCCACAAACGTAGAGAATTCCAGCAAAAAGGGAAGGAGGAACCAAAAGGGCAAAAATCGGGGATGT GTCTTAACAGAAATACATTTAAATGTGACTGACTTGGATTTGGGATACGAAACCAAAGAAGAGCTCATCTTCCGGTATT GCAGTGGATCTTGTGATGCAGTTGAGACCACGTATGACAAAATTTTGAAAAACTTAACCAGAAAGAAAAAACTAGTCAA TGACAAAGTGAGGCAAGCTTGCTGCAGACCCACAGCCTTTGACGATGACCTGTCCTTTTTGGATGATAACCTGGTTTAC CACATACTGAAAAAACATTCCGCAAAAAGGTGTGGATGTGTCTGA
Citation	Burns AJ, Delalande JM (2005). Neural crest cell origin for intrinsic ganglia of the developing chicken lung. Dev Biol. 2005 Jan 1;277(1):63-79.
Copyright	Copyright © 2004 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (XM_425018).