Geisha

home

contact

A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

Quick Search

Data Class

Enter Text:

Gene Family QuickSearch

	Transcription Factors
	Growth Factors
	Receptors
	MicroRNAs

Human Disease Gene Search

	Downloads
	Protocols
	About GEISHA
	Contact Us

	Transgenic Birds
	Model Organism Databases
	Gene Expression Databases
	Genomic Resources
	Anatomical Atlases
	Chicken Stage Series

GFRA4
GDNF family receptor alpha 4

Gene

Official Symbol	GFRA4
Official Full Name	GDNF family receptor alpha 4
CGNC ID	49506
also known as	GDNF family receptor alpha-4\|GDNF receptor alpha-4\|GDNFR-alpha-4\|GFR receptor alpha 4\|GFR-alpha-4, GFR-alpha-4, GDNF family receptor alpha-4 precursor
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_204991
Polypeptide	NP_990322

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus	100487002			854947
Zebrafish	565651, 100144564	ENSDARG00000074582, ENSDARG00000056651	ZDB-GENE-080402-10	ZFIN:ZDB-GENE-080402-10

Gene Ontology

Molecular Function	receptor activity
Biological Process
Cellular Component	anchored component of membrane, plasma membrane

Links to other databases

Entrez Gene	395839
Ensembl Gene
KEGG	gga:395839

Entry 1

GEISHA Id	GFRA4.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATCGGCAGTGTATCGCAGGTAATG
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGCAATGTAGTTGGGAGTGATGGGG	cDNA Template Length	748
Complete cDNA Template Probe	show CGGCAGTGTATCGCAGGTAATGGAGCCAACAAGCTGGGCCCCGACGCCAAGAACCAGTGTCGGAGCACAGTGACTGCCC TGCTCTCCAGCCAGCTCTACGGCTGCAAGTGCAAGCGAGGCATGAAAAAGGAGAAGCATTGCTTGAGTGTCTACTGGAG CATCCACCATACGCTGATGGAAGGCATGAACGTGCTGGAAAGTTCTCCTTATGAGCCATTCATCAGGGGCTTTGATTAT GTCCGGCTGGCCTCCATCACTGCAGGGTCTGAGAACGAGGTGACCCAGGTGAACCGCTGCCTGGACGCCGCCAAAGCCT GCAACGTGGACGAGATGTGCCAACGGCTGCGGACGGAATATGTCTCCTTCTGCATCCGGCGCCTGGCCCGGGCCGACAC CTGCAACCGCTCCAAGTGCCATAAGGCCTTACGCAAGTTCTTCGACCGCGTGCCGCCCGAGTACACCCACGAGCTGCTC TTCTGCCCCTGCGAAGACACAGCCTGTGCTGAACGCCGGCGGCAGACCATCGTTCCGGCCTGCTCCTATGAGTCCAAGG AGAAACCCAACTGCCTGGCACCGCTGGACTCCTGCCGCGAGAACTACGTCTGTAGGTCGCGTTATGCAGAGTTCCAGTT TAATTGCCAACCATCCCTGCAGACTGCGAGTGGCTGCCGGAGGGACAGCTATGCTGCCTGCCTGCTGGCTTACACCGGG ATCATAGGCAGCCCCATCACTCCCAACTACATTG

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 6

Area Pellucida
Headfold
Hensen's Node
Lateral Plate Mesoderm
Paraxial Mesoderm
Primitive Streak

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

Area Opaca
Forebrain
Head Mesenchyme
Headfold
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Notochord
Paraxial Mesoderm
Primitive Streak
Somites

Dorsal side.
Ventral side.
Ventral view.

13-18

stage 13

stage 14

stage 16

stage 17

Cranial Ganglion
Cranial PNS
Ear/Otic Placcode
Eye
Head Mesenchyme
Intermediate Mesoderm
Neural Plate/Tube
Notochord
Paraxial Mesoderm
Somites
Spinal Cord
Spinal Ganglia and Nerves

Dorsal view.

19-24

stage 22

stage 23

Leg
Midbrain
Paraxial Mesoderm
Somites
Spinal Cord
Wing

stage 25

Atria
Ear/Otic Placcode
Head Mesenchyme
Heart
Hindbrain
Leg Mesenchyme
Limb Buds
Midbrain
Somites
Spinal Cord
Tail
Wing Mesenchyme

Entry 2

GEISHA Id	GFRA4.Rothermel.2004	Data Source	Publication
Complete cDNA Template Probe	show CAGGGGCTTTGATTATGTCCGGCTGGCCTCCATCACTGCAGGGTCTGAGAACGAGGTGACCCAGGTGAACCGCTGCCTG GACGCCGCCAAAGCCTGCAACGTGGACGAGATGTGCCAACGGCTGCGGACGGAATATGTCTCCTTCTGCATCCGGCGCC TGGCCCGGGCCGACACCTGCAACCGCTCCAAGTGCCATAAGGCCTTACGCAAGTTCTTCGACCGCGTGCCGCCCGAGTA CACCCACGAGCTGCTCTTCTGCCCCTGCGAAGACACAGCCTGTGCTGAACGCCGGCGGCAGACCATCGTTCCGGCCTGC TCCTATGAGTCCAAGGAGAAACCCAACTGCCTGGCACCGCTGGACTCCTGCCGCGAGAACTACGTCTGTAGGTCGCGTT ATGCAGAGTTCCAGTTTAATTGCCAACCATCCCTGCAGACTGCGAGTGGCTGCCGGAGGGACAGCTATGCTGCCTGCCT GCTGGCTTACACCGGGATCATAGGCAGCCCCATCACTCCCAACTACATTGACAACTCGACCTCCAGCATAGCACCCTGG TGCACGTGCAACGCAAGTGGCAACCGGCAGGAAGAGTGCGAGAGCTT
Citation	Rothermel A, Volpert K, Schlichting R, Huhn J, Stotz-Reimers M, Robitzki AA, Layer PG. Spatial and temporal expression patterns of GDNF family receptor alpha4 in the developing chicken retina. Gene Expr Patterns. 2004 Jan;4(1):59-63.
Copyright	Reprinted with permission from Elsevier. Copyright © 2003 Elsevier B.V. All rights reserved.
Comments	According to the authors, " The primers for GFRα4 are 5'-CAGGGGCTTTGATTATGTCC-3' and 5'-AAGCTCTCGCACTCTTCCTG-3', which correspond to nucleotide positions 360379 and 940959 (GenBank Accession Number AF045162)."

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 23

Unlabeled

25-30

stage 28

Retina
Unlabeled

Ganglion Cell Layer

31-44

stage 34

stage 38

stage 40

stage 42

stage 44

Retina
Unlabeled

Ganglion Cell Layer
Hybridization using the sense GFRα4 riboprobe showed no signal.
Inner Nuclear Layer
Neuroepithelial Cells of Ciliary Marginal Zone
Ora serrata
Outer Nuclear Layer
Photoreceptors
Retinal Periphery
sense

Entry 3

GEISHA Id	GFRA4.Homma.2000	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCATCCTCTACTTCTGCACGCTGATCCTCCTGGAAGGTATGGCAGAAGCTGTCAGCTCAAGCAGGGACTGCC TTCAGGCAGGTGAGTCCTGCACCAACGACCCCATCTGCAGCTCCAAATTCAGGACCCTCCGGCAGTGTATCGCAGGTAA TGGAGCCAACAAGCTGGGCCCCGACGCCAAGAACCAGTGTCGGAGCACAGTGACTGCCCTGCTCTCCAGCCAGCTCTAC GGCTGCAAGTGCAAGCGAGGCATGAAAAAGGAGAAGCATTGCTTGAGTGTCTACTGGAGCATCCACCATACGCTGATGG AAGGCATGAACGTGCTGGAAAGTTCTCCTTATGAGCCATTCATCAGGGGCTTTGATTATGTCCGGCTGGCCTCCATCAC TGCAGGGTCTGAGAACGAGGTGACCCAGGTGAACCGCTGCCTGGACGCCGCCAAAGCCTGCAACGTGGACGAGATGTGC CAACGGCTGCGGACGGAATATGTCTCCTTCTGCATCCGGCGCCTGGCCCGGGCCGACACCTGCAACCGCTCCAAGTGCC ATAAGGCCTTACGCAAGTTCTTCGACCGCGTGCCGCCCGAGTACACCCACGAGCTGCTCTTCTGCCCCTGCGAAGACAC AGCCTGTGCTGAACGCCGGCGGCAGACCATCGTTCCGGCCTGCTCCTATGAGTCCAAGGAGAAACCCAACTGCCTGGCA CCGCTGGACTCCTGCCGCGAGAACTACGTCTGTAGGTCGCGTTATGCAGAGTTCCAGTTTAATTGCCAACCATCCCTGC AGACTGCGAGTGGCTGCCGGAGGGACAGCTATGCTGCCTGCCTGCTGGCTTACACCGGGATCATAGGCAGCCCCATCAC TCCCAACTACATTGACAACTCGACCTCCAGCATAGCACCCTGGTGCACGTGCAACGCAAGTGGCAACCGGCAGGAAGAG TGCGAGAGCTTCCTGCACCTCTTCACTGACAACGTCTGTCTCCAAAATGCCATCCAGGCCTTTGGCAACGGGACCTACC TGAACGCAGCCACAGCCCCATCCATCTCCCCCACCACGCAGATGTACAAGCAGGAGCGAAACGCCAACAGAGCTGCAGC AACCCTCAGCGAGAACATCTTCGAGCACCTCCAGCCTACCAAGGTGGCTGGAGAGGAGAGGCTCCTGCGGGGCTCCACT CGTCTGTCATCAGAGACCTCCAGCCCAGCAGCTCCTTGCCACCAGGCAGCCTCCCTGCTGCAGCTGTGGCTTCCCCCCA CTCTTGCTGTGCTGAGCCACTTCATGATGTGA
Citation	Homma S, Oppenheim RW, Yaginuma H, Kimura S. Expression pattern of GDNF, c-ret, and GFRalphas suggests novel roles for GDNF ligands during early organogenesis in the chick embryo. Dev Biol. 2000 Jan 1;217(1):121-37.
Copyright	Reprinted with permission from Elsevier. Copyright © 2000 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204991).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 9

Hensen's Node
Notochord
Somites

13-18

stage 14

stage 18

Cranial Ganglion
Dermomyotome
Ear/Otic Placcode
Somites
Spinal Cord
Splanchnic Mesoderm

Motoneurons
Ophthalmic and Maxillo-Mandibular Divisions of the Trigeminal Ganglion (V)
Ventral Lip of the Dermamyotome
Vestibuloacoustic Ganglion (VIII)

Entry 4

GEISHA Id	GFRα4.Tatsumi.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCATCCTCTACTTCTGCACGCTGATCCTCCTGGAAGGTATGGCAGAAGCTGTCAGCTCAAGCAGGGACTGCC TTCAGGCAGGTGAGTCCTGCACCAACGACCCCATCTGCAGCTCCAAATTCAGGACCCTCCGGCAGTGTATCGCAGGTAA TGGAGCCAACAAGCTGGGCCCCGACGCCAAGAACCAGTGTCGGAGCACAGTGACTGCCCTGCTCTCCAGCCAGCTCTAC GGCTGCAAGTGCAAGCGAGGCATGAAAAAGGAGAAGCATTGCTTGAGTGTCTACTGGAGCATCCACCATACGCTGATGG AAGGCATGAACGTGCTGGAAAGTTCTCCTTATGAGCCATTCATCAGGGGCTTTGATTATGTCCGGCTGGCCTCCATCAC TGCAGGGTCTGAGAACGAGGTGACCCAGGTGAACCGCTGCCTGGACGCCGCCAAAGCCTGCAACGTGGACGAGATGTGC CAACGGCTGCGGACGGAATATGTCTCCTTCTGCATCCGGCGCCTGGCCCGGGCCGACACCTGCAACCGCTCCAAGTGCC ATAAGGCCTTACGCAAGTTCTTCGACCGCGTGCCGCCCGAGTACACCCACGAGCTGCTCTTCTGCCCCTGCGAAGACAC AGCCTGTGCTGAACGCCGGCGGCAGACCATCGTTCCGGCCTGCTCCTATGAGTCCAAGGAGAAACCCAACTGCCTGGCA CCGCTGGACTCCTGCCGCGAGAACTACGTCTGTAGGTCGCGTTATGCAGAGTTCCAGTTTAATTGCCAACCATCCCTGC AGACTGCGAGTGGCTGCCGGAGGGACAGCTATGCTGCCTGCCTGCTGGCTTACACCGGGATCATAGGCAGCCCCATCAC TCCCAACTACATTGACAACTCGACCTCCAGCATAGCACCCTGGTGCACGTGCAACGCAAGTGGCAACCGGCAGGAAGAG TGCGAGAGCTTCCTGCACCTCTTCACTGACAACGTCTGTCTCCAAAATGCCATCCAGGCCTTTGGCAACGGGACCTACC TGAACGCAGCCACAGCCCCATCCATCTCCCCCACCACGCAGATGTACAAGCAGGAGCGAAACGCCAACAGAGCTGCAGC AACCCTCAGCGAGAACATCTTCGAGCACCTCCAGCCTACCAAGGTGGCTGGAGAGGAGAGGCTCCTGCGGGGCTCCACT CGTCTGTCATCAGAGACCTCCAGCCCAGCAGCTCCTTGCCACCAGGCAGCCTCCCTGCTGCAGCTGTGGCTTCCCCCCA CTCTTGCTGTGCTGAGCCACTTCATGATGTGA
Citation	Tatsumi N, Miki R, Katsu K, Yokouchi Y. Neurturin-GFRalpha2 signaling controls liver bud migration along the ductus venosus in the chick embryo. Dev Biol. 2007 Jul 1;307(1):14-28. Epub 2007 Apr 3.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Authors said: GFRα4 (Genbank accession no. AF045162)