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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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FOXA2
forkhead box A2

Gene

Official Symbol	FOXA2
Official Full Name	forkhead box A2
CGNC ID	49366
also known as	HNF-3BETA, hepatocyte nuclear factor 3-beta\|hepatocyte nuclear factor-3beta\|transcription factor Foxa2, FOX2A, HNF-3b , HNF3b
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_204770
Polypeptide	NP_990101

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	395539
Ensembl Gene
KEGG	gga:395539

Entry 1

GEISHA Id	HNF-3b	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGCATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGC CAACATGACGGCAGCCACCTCCATGAACATGTCCTACGCCAACACGGGCATGAGCCCTTCACTCGCCGGCATGTCCCCA GGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGTGGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCA TGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCCTACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATC CAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGCGGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCG CTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGATGCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCT TCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAACAGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAA GGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCTTCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAAC GGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGAGAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGG GGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGCAGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTC ACCAGCGAGTGCCTCGCCATGCCGGGACCACAAGCGTGCCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCC GGGGAGCCGTCGGCGGCCTCTCCAGCGCAGCACCTGCTGGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCA AGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCCATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCA CCACCCGCACCACCACCACCACCCCTCGCACAAAATGGACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTAC GCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGTAACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAG AGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATCATGAACTCCTCCTAA

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Hensen's Node

7-9

stage 8

stage 9

Anterior Intestinal Portal
Notochord

Entry 2

GEISHA Id	Hnf-3beta.Yanai.2005	Data Source	Publication
Complete cDNA Template Probe	show `GTGGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGATCTCCATGGGAGGACAAGCGGGCTCTATGAACGCCCTGGCCC CCTACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCG GCGGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAG ATGCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAGCGCTGGCAGA ACAGCATCCGCCACTCGCTTTCCTTCAATGACTG`
Citation	Yanai M, Tatsumi N, Endo F, Yokouchi Y. Analysis of gene expression patterns in the developing chick liver. Dev Dyn. 2005 Jul;233(3):1116-22.
Copyright	Wiley-Liss, Inc. 2005

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Anterior Intestinal Portal
Endoderm

13-18

stage 17

Liver
Pancreas

19-24

stage 22

Duodenum
Liver
Pancreas

25-30

stage 25

Liver
Pancreas

cystic duct

31-42

stage 42

Anterior Intestinal Portal
Endoderm
Liver
Pancreas
Splanchnic Mesoderm

extrahepatic bile ducts, gall bladder epithelium

Entry 3

GEISHA Id	HNF3b.Chapman.2002	Data Source	Publication
Complete cDNA Template Probe	show CGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGCATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGC CAACATGACGGCAGCCACCTCCATGAACATGTCCTACGCCAACACGGGCATGAGCCCTTCACTCGCCGGCATGTCCCCA GGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGTGGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCA TGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCCTACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATC CAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGCGGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCG CTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGATGCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCT TCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAACAGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAA GGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCTTCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAAC GGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGAGAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGG GGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGCAGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTC ACCAGCGAGTGCCTCGCCATGCCGGGACCACAAGCGTGCCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCC GGGGAGCCGTCGGCGGCCTCTCCAGCGCAGCACCTGCTGGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCA AGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCCATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCA CCACCCGCACCACCACCACCACCCCTCGCACAAAATGGACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTAC GCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGTAACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAG AGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATCATGAACTCCTCCTAA
Citation	Chapman SC, Schubert FR, Schoenwolf GC, Lumsden A. Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos. Dev Biol. 2002 May 1;245(1):187-99.
Copyright	2002 Elsevier Science (USA). All rights reserved.

Stage

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Location (click to highlight)

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X-XIV

stage XII

Hypoblast
Kohler's Sickle

1-3

stage 3

Endoderm
Germinal Crescent
Head Process
Hensen's Node
Notochord

4-6

stage 4

stage 5

stage 6

Ectoderm
Endoderm
Head Process
Hensen's Node
Notochord
Primitive Streak

Entry 4

GEISHA Id	Hnf3β.Abe.2006	Data Source	Publication
Complete cDNA Template Probe	show TTAATGGCTGATCTGGGAGAGACAGAGCACCTGTAGACCAGGCATAATAATTGTACGCTAGAAGATGCAGAAGTCCTGC CAGTAACTAAAAGAGGGAGTCCATCTGCATCTTCTAGCGTACAAATATTATGCCTGGTCTACAGGTGCTCTGTCTCTCC CAGATCAGCCATTAACTGAACCCCCTGGTGGCCTCAGAACGGAATTCCGGAATTCCACCGAGGGAAAAGGTCGGTGCCG CGCTGCCCCGCCTGGTGCCCGGGCAGGTACCTGAGGCAGAGCCGCGGGAAGGCATGAGGCAGAGCCGGGCCAGGGCCGG GAGGAAGGCGGCCTGAGGCCGAGCAGCCGCGGGGAGAAAGTATGCTGGGAGCGGTGAAAATGGAAGGCCATGAGCACAC GGACTGGAGCAACTACTACGGGGAGCCCGAGAGCTACTCCTCGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGC ATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGCCAACATGACGGCAGCCACCTCCATGAACATGTCCTACG CCAACACAGGCATGAGCCCTTCACTCGCCGGCATGTCCCCAGGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGT GGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCATGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCC TACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGA GGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGAT GCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAAC AGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAAGGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCT TCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAACGGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGA GAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGGGGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGC AGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTCACCAGCGAGTGCCTCGCCATGCCGGGACAACAAGCGTG CCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCCGGGGAGCCGTCGGCGGCCTCTCCAGCGCATCACCTGCT GGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCAAGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCC ATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCACCACCCGCACCACCACCACCACCCCTCGCACAAAATGG ACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTACGCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGT AACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAGAGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATC ATGAACTCCTCCTAAAGGCAAAGGGGGAAAAACCCCCCCACCCCGCGCGGTCCCCTAGCAGCGTGATGGACTGTGAGCG CTGCCGAGCACGGTACCTACCCATATAGCTATCTATCTAGTTTTTTGTTTTGTTCCCTGCTGGCTCCAGGTGGTTGCAT CCACACGGGGAGCAGCGGGCGCACCTCTGCCTGGCACCCCCTAACCCTGACCCTGCGGTCCCCTCGGTTTGCCTTGCGG GGTGGTTCGGTTTGGTGGTGGGGGGTGTAGGGGAGAGGGTATTTTCTGTTGGGGCTGGTTTCTTTATTTTCGTTGTCGT TGTCGCAAGGAGGTCTAAATATATCTATTTTTTTGTGTGGAAGTGATGGGGAAAAGAGAAAAACAAACAGACAAAAATG GACGCAAAGAAAACGAAGCCCTCGTTCGGTGGGAAATCCGTGCGAGTTTGGGATCTGAGGATGGGAAGTCTTGTAAATT GTACAAAGTCGAC
Citation	Abe Y, Chen W, Huang W, Nishino M, Li YP. CNBP regulates forebrain formation at organogenesis stage in chick embryos. Dev Biol. 2006 Jul 1;295(1):116-27. Epub 2006 Apr 19.
Copyright	Reprinted from Developmental Biology, 295, Yoko Abea, Wei Chena, Wei Huangc, Mizuho Nishinod and Yi-Ping Li, CNBP regulates forebrain formation at organogenesis stage in chick embryos, 116-127, Copyright 2006, with permission from Elsevier. Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	Authors said: En1 and Hnf3β cDNA were linearized and transcribed with T7-RNA polymerase. As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #AF150749)

Stage

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stage 19

Forebrain
Head Mesenchyme

The figure caption has been modified to reflect the control result.

Entry 5

GEISHA Id	Hnf3b.Chapman.2008	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGCATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGC CAACATGACGGCAGCCACCTCCATGAACATGTCCTACGCCAACACGGGCATGAGCCCTTCACTCGCCGGCATGTCCCCA GGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGTGGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCA TGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCCTACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATC CAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGCGGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCG CTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGATGCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCT TCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAACAGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAA GGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCTTCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAAC GGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGAGAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGG GGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGCAGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTC ACCAGCGAGTGCCTCGCCATGCCGGGACCACAAGCGTGCCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCC GGGGAGCCGTCGGCGGCCTCTCCAGCGCAGCACCTGCTGGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCA AGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCCATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCA CCACCCGCACCACCACCACCACCCCTCGCACAAAATGGACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTAC GCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGTAACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAG AGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATCATGAACTCCTCCTAA
Comments	Images Provided by Susan Chapman

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XII

Area Pellucida

1-3

stage 3

Hensen's Node
Primitive Streak

4-6

stage 4

stage 5

stage 6

Early Mesoderm
Head Mesenchyme
Head Process
Headfold
Hensen's Node
Notochord
Primitive Streak

7-12

stage 8

stage 9

stage 10

Anterior Intestinal Portal
Head Process
Headfold
Hensen's Node
Lateral Plate Mesoderm
Notochord

13-17

stage 17

Gut
Head Process
Notochord

Entry 6

GEISHA Id	FOXA2.Agarwala.2005	Data Source	Publication
Complete cDNA Template Probe	show TTAATGGCTGATCTGGGAGAGACAGAGCACCTGTAGACCAGGCATAATAATTGTACGCTAGAAGATGCAGAAGTCCTGC CAGTAACTAAAAGAGGGAGTCCATCTGCATCTTCTAGCGTACAAATATTATGCCTGGTCTACAGGTGCTCTGTCTCTCC CAGATCAGCCATTAACTGAACCCCCTGGTGGCCTCAGAACGGAATTCCGGAATTCCACCGAGGGAAAAGGTCGGTGCCG CGCTGCCCCGCCTGGTGCCCGGGCAGGTACCTGAGGCAGAGCCGCGGGAAGGCATGAGGCAGAGCCGGGCCAGGGCCGG GAGGAAGGCGGCCTGAGGCCGAGCAGCCGCGGGGAGAAAGTATGCTGGGAGCGGTGAAAATGGAAGGCCATGAGCACAC GGACTGGAGCAACTACTACGGGGAGCCCGAGAGCTACTCCTCGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGC ATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGCCAACATGACGGCAGCCACCTCCATGAACATGTCCTACG CCAACACAGGCATGAGCCCTTCACTCGCCGGCATGTCCCCAGGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGT GGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCATGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCC TACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGA GGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGAT GCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAAC AGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAAGGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCT TCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAACGGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGA GAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGGGGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGC AGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTCACCAGCGAGTGCCTCGCCATGCCGGGACAACAAGCGTG CCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCCGGGGAGCCGTCGGCGGCCTCTCCAGCGCATCACCTGCT GGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCAAGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCC ATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCACCACCCGCACCACCACCACCACCCCTCGCACAAAATGG ACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTACGCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGT AACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAGAGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATC ATGAACTCCTCCTAAAGGCAAAGGGGGAAAAACCCCCCCACCCCGCGCGGTCCCCTAGCAGCGTGATGGACTGTGAGCG CTGCCGAGCACGGTACCTACCCATATAGCTATCTATCTAGTTTTTTGTTTTGTTCCCTGCTGGCTCCAGGTGGTTGCAT CCACACGGGGAGCAGCGGGCGCACCTCTGCCTGGCACCCCCTAACCCTGACCCTGCGGTCCCCTCGGTTTGCCTTGCGG GGTGGTTCGGTTTGGTGGTGGGGGGTGTAGGGGAGAGGGTATTTTCTGTTGGGGCTGGTTTCTTTATTTTCGTTGTCGT TGTCGCAAGGAGGTCTAAATATATCTATTTTTTTGTGTGGAAGTGATGGGGAAAAGAGAAAAACAAACAGACAAAAATG GACGCAAAGAAAACGAAGCCCTCGTTCGGTGGGAAATCCGTGCGAGTTTGGGATCTGAGGATGGGAAGTCTTGTAAATT GTACAAAGTCGAC
Citation	Agarwala S, Aglyamova GV, Marma AK, Fallon JF, Ragsdale CW. Differential susceptibility of midbrain and spinal cord patterning to floor plate defects in the talpid2 mutant. Dev Biol. 2005 Dec 1;288(1):206-20. Epub 2005 Oct 21.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204770).

Stage

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Location (click to highlight)

Comments (click to highlight)

25-26

stage 26

Midbrain

Medial Floor Plate

Entry 7

GEISHA Id	HNF-3BETA.Foley.2000	Data Source	Publication
Complete cDNA Template Probe	show TTAATGGCTGATCTGGGAGAGACAGAGCACCTGTAGACCAGGCATAATAATTGTACGCTAGAAGATGCAGAAGTCCTGC CAGTAACTAAAAGAGGGAGTCCATCTGCATCTTCTAGCGTACAAATATTATGCCTGGTCTACAGGTGCTCTGTCTCTCC CAGATCAGCCATTAACTGAACCCCCTGGTGGCCTCAGAACGGAATTCCGGAATTCCACCGAGGGAAAAGGTCGGTGCCG CGCTGCCCCGCCTGGTGCCCGGGCAGGTACCTGAGGCAGAGCCGCGGGAAGGCATGAGGCAGAGCCGGGCCAGGGCCGG GAGGAAGGCGGCCTGAGGCCGAGCAGCCGCGGGGAGAAAGTATGCTGGGAGCGGTGAAAATGGAAGGCCATGAGCACAC GGACTGGAGCAACTACTACGGGGAGCCCGAGAGCTACTCCTCGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGC ATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGCCAACATGACGGCAGCCACCTCCATGAACATGTCCTACG CCAACACAGGCATGAGCCCTTCACTCGCCGGCATGTCCCCAGGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGT GGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCATGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCC TACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGA GGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGAT GCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAAC AGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAAGGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCT TCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAACGGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGA GAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGGGGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGC AGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTCACCAGCGAGTGCCTCGCCATGCCGGGACAACAAGCGTG CCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCCGGGGAGCCGTCGGCGGCCTCTCCAGCGCATCACCTGCT GGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCAAGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCC ATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCACCACCCGCACCACCACCACCACCCCTCGCACAAAATGG ACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTACGCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGT AACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAGAGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATC ATGAACTCCTCCTAAAGGCAAAGGGGGAAAAACCCCCCCACCCCGCGCGGTCCCCTAGCAGCGTGATGGACTGTGAGCG CTGCCGAGCACGGTACCTACCCATATAGCTATCTATCTAGTTTTTTGTTTTGTTCCCTGCTGGCTCCAGGTGGTTGCAT CCACACGGGGAGCAGCGGGCGCACCTCTGCCTGGCACCCCCTAACCCTGACCCTGCGGTCCCCTCGGTTTGCCTTGCGG GGTGGTTCGGTTTGGTGGTGGGGGGTGTAGGGGAGAGGGTATTTTCTGTTGGGGCTGGTTTCTTTATTTTCGTTGTCGT TGTCGCAAGGAGGTCTAAATATATCTATTTTTTTGTGTGGAAGTGATGGGGAAAAGAGAAAAACAAACAGACAAAAATG GACGCAAAGAAAACGAAGCCCTCGTTCGGTGGGAAATCCGTGCGAGTTTGGGATCTGAGGATGGGAAGTCTTGTAAATT GTACAAAGTCGAC
Citation	Foley AC, Skromne I, Stern CD. Reconciling different models of forebrain induction and patterning: a dual role for the hypoblast. Development. 2000 Sep;127(17):3839-54.
Copyright	Printed in Great Britain © The Company of Biologists Limited 2000
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204770).

Stage

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Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XII

Hypoblast

Stage XII-XIII EG&K

1-3

stage 2

Hypoblast

stage 4

Hypoblast
Primitive Streak

Entry 8

GEISHA Id	HNF3-B.Levin.1995	Data Source	Publication
Complete cDNA Template Probe	show TTAATGGCTGATCTGGGAGAGACAGAGCACCTGTAGACCAGGCATAATAATTGTACGCTAGAAGATGCAGAAGTCCTGC CAGTAACTAAAAGAGGGAGTCCATCTGCATCTTCTAGCGTACAAATATTATGCCTGGTCTACAGGTGCTCTGTCTCTCC CAGATCAGCCATTAACTGAACCCCCTGGTGGCCTCAGAACGGAATTCCGGAATTCCACCGAGGGAAAAGGTCGGTGCCG CGCTGCCCCGCCTGGTGCCCGGGCAGGTACCTGAGGCAGAGCCGCGGGAAGGCATGAGGCAGAGCCGGGCCAGGGCCGG GAGGAAGGCGGCCTGAGGCCGAGCAGCCGCGGGGAGAAAGTATGCTGGGAGCGGTGAAAATGGAAGGCCATGAGCACAC GGACTGGAGCAACTACTACGGGGAGCCCGAGAGCTACTCCTCGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGC ATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGCCAACATGACGGCAGCCACCTCCATGAACATGTCCTACG CCAACACAGGCATGAGCCCTTCACTCGCCGGCATGTCCCCAGGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGT GGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCATGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCC TACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGA GGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGAT GCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAAC AGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAAGGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCT TCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAACGGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGA GAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGGGGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGC AGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTCACCAGCGAGTGCCTCGCCATGCCGGGACAACAAGCGTG CCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCCGGGGAGCCGTCGGCGGCCTCTCCAGCGCATCACCTGCT GGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCAAGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCC ATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCACCACCCGCACCACCACCACCACCCCTCGCACAAAATGG ACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTACGCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGT AACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAGAGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATC ATGAACTCCTCCTAAAGGCAAAGGGGGAAAAACCCCCCCACCCCGCGCGGTCCCCTAGCAGCGTGATGGACTGTGAGCG CTGCCGAGCACGGTACCTACCCATATAGCTATCTATCTAGTTTTTTGTTTTGTTCCCTGCTGGCTCCAGGTGGTTGCAT CCACACGGGGAGCAGCGGGCGCACCTCTGCCTGGCACCCCCTAACCCTGACCCTGCGGTCCCCTCGGTTTGCCTTGCGG GGTGGTTCGGTTTGGTGGTGGGGGGTGTAGGGGAGAGGGTATTTTCTGTTGGGGCTGGTTTCTTTATTTTCGTTGTCGT TGTCGCAAGGAGGTCTAAATATATCTATTTTTTTGTGTGGAAGTGATGGGGAAAAGAGAAAAACAAACAGACAAAAATG GACGCAAAGAAAACGAAGCCCTCGTTCGGTGGGAAATCCGTGCGAGTTTGGGATCTGAGGATGGGAAGTCTTGTAAATT GTACAAAGTCGAC
Citation	Levin M, Johnson RL, Stern CD, Kuehn M, Tabin C (1995). A molecular pathway determining left-right asymmetry in chick embryogenesis. Cell. 1995 Sep 8;82(5):803-14.
Copyright	Copyright © 1995 Elsevier Inc. All Rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204770.1)

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Hensen's Node

31-44

stage 44

Entry 9

GEISHA Id	FOXA2.Agarwala.2001	Data Source	Publication
Complete cDNA Template Probe	show TTAATGGCTGATCTGGGAGAGACAGAGCACCTGTAGACCAGGCATAATAATTGTACGCTAGAAGATGCAGAAGTCCTGC CAGTAACTAAAAGAGGGAGTCCATCTGCATCTTCTAGCGTACAAATATTATGCCTGGTCTACAGGTGCTCTGTCTCTCC CAGATCAGCCATTAACTGAACCCCCTGGTGGCCTCAGAACGGAATTCCGGAATTCCACCGAGGGAAAAGGTCGGTGCCG CGCTGCCCCGCCTGGTGCCCGGGCAGGTACCTGAGGCAGAGCCGCGGGAAGGCATGAGGCAGAGCCGGGCCAGGGCCGG GAGGAAGGCGGCCTGAGGCCGAGCAGCCGCGGGGAGAAAGTATGCTGGGAGCGGTGAAAATGGAAGGCCATGAGCACAC GGACTGGAGCAACTACTACGGGGAGCCCGAGAGCTACTCCTCGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGC ATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGCCAACATGACGGCAGCCACCTCCATGAACATGTCCTACG CCAACACAGGCATGAGCCCTTCACTCGCCGGCATGTCCCCAGGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGT GGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCATGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCC TACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGA GGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGAT GCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAAC AGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAAGGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCT TCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAACGGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGA GAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGGGGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGC AGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTCACCAGCGAGTGCCTCGCCATGCCGGGACAACAAGCGTG CCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCCGGGGAGCCGTCGGCGGCCTCTCCAGCGCATCACCTGCT GGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCAAGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCC ATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCACCACCCGCACCACCACCACCACCCCTCGCACAAAATGG ACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTACGCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGT AACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAGAGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATC ATGAACTCCTCCTAAAGGCAAAGGGGGAAAAACCCCCCCACCCCGCGCGGTCCCCTAGCAGCGTGATGGACTGTGAGCG CTGCCGAGCACGGTACCTACCCATATAGCTATCTATCTAGTTTTTTGTTTTGTTCCCTGCTGGCTCCAGGTGGTTGCAT CCACACGGGGAGCAGCGGGCGCACCTCTGCCTGGCACCCCCTAACCCTGACCCTGCGGTCCCCTCGGTTTGCCTTGCGG GGTGGTTCGGTTTGGTGGTGGGGGGTGTAGGGGAGAGGGTATTTTCTGTTGGGGCTGGTTTCTTTATTTTCGTTGTCGT TGTCGCAAGGAGGTCTAAATATATCTATTTTTTTGTGTGGAAGTGATGGGGAAAAGAGAAAAACAAACAGACAAAAATG GACGCAAAGAAAACGAAGCCCTCGTTCGGTGGGAAATCCGTGCGAGTTTGGGATCTGAGGATGGGAAGTCTTGTAAATT GTACAAAGTCGAC
Citation	Agarwala S, Sanders TA, Ragsdale CW. Sonic hedgehog control of size and shape in midbrain pattern formation. Science. 2001 Mar 16;291(5511):2147-50. Epub 2001 Mar 1.
Copyright	© 2011 American Association for the Advancement of Science. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc NM_204770.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

Midbrain

Normal on the left, double labeled
Normal on left, double labeled
Normal on the left, double labeled
normal on left, double labeled

13-18

stage 13

Forebrain
Hindbrain
Isthmus/MHB
Midbrain

Double label

25-26

stage 26

Forebrain
Hindbrain
Isthmus/MHB
Midbrain

Double label

Entry 10

GEISHA Id	FOXA2.GarciaCampmany.2007	Data Source	Publication
Complete cDNA Template Probe	show TTAATGGCTGATCTGGGAGAGACAGAGCACCTGTAGACCAGGCATAATAATTGTACGCTAGAAGATGCAGAAGTCCTGC CAGTAACTAAAAGAGGGAGTCCATCTGCATCTTCTAGCGTACAAATATTATGCCTGGTCTACAGGTGCTCTGTCTCTCC CAGATCAGCCATTAACTGAACCCCCTGGTGGCCTCAGAACGGAATTCCGGAATTCCACCGAGGGAAAAGGTCGGTGCCG CGCTGCCCCGCCTGGTGCCCGGGCAGGTACCTGAGGCAGAGCCGCGGGAAGGCATGAGGCAGAGCCGGGCCAGGGCCGG GAGGAAGGCGGCCTGAGGCCGAGCAGCCGCGGGGAGAAAGTATGCTGGGAGCGGTGAAAATGGAAGGCCATGAGCACAC GGACTGGAGCAACTACTACGGGGAGCCCGAGAGCTACTCCTCGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAGC ATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGCCAACATGACGGCAGCCACCTCCATGAACATGTCCTACG CCAACACAGGCATGAGCCCTTCACTCGCCGGCATGTCCCCAGGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCGT GGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCATGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCCC TACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGGA GGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGAT GCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAACGCTGGCAGAAC AGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAAGGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCCT TCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAACGGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTGA GAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGGGGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGGC AGCTCCTCGGGGGGCTCCGAGGGCTCGGCGGGAGCTGAGTCACCAGCGAGTGCCTCGCCATGCCGGGACAACAAGCGTG CCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCCGGGGAGCCGTCGGCGGCCTCTCCAGCGCATCACCTGCT GGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCAAGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTCC ATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCACCACCCGCACCACCACCACCACCCCTCGCACAAAATGG ACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTACGCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCGT AACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAGAGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCATC ATGAACTCCTCCTAAAGGCAAAGGGGGAAAAACCCCCCCACCCCGCGCGGTCCCCTAGCAGCGTGATGGACTGTGAGCG CTGCCGAGCACGGTACCTACCCATATAGCTATCTATCTAGTTTTTTGTTTTGTTCCCTGCTGGCTCCAGGTGGTTGCAT CCACACGGGGAGCAGCGGGCGCACCTCTGCCTGGCACCCCCTAACCCTGACCCTGCGGTCCCCTCGGTTTGCCTTGCGG GGTGGTTCGGTTTGGTGGTGGGGGGTGTAGGGGAGAGGGTATTTTCTGTTGGGGCTGGTTTCTTTATTTTCGTTGTCGT TGTCGCAAGGAGGTCTAAATATATCTATTTTTTTGTGTGGAAGTGATGGGGAAAAGAGAAAAACAAACAGACAAAAATG GACGCAAAGAAAACGAAGCCCTCGTTCGGTGGGAAATCCGTGCGAGTTTGGGATCTGAGGATGGGAAGTCTTGTAAATT GTACAAAGTCGAC
Citation	García-Campmany L, Martí E. The TGFbeta intracellular effector Smad3 regulates neuronal differentiation and cell fate specification in the developing spinal cord. Development. 2007 Jan;134(1):65-75. Epub 2006 Nov 30.
Copyright	Copyright by the authors. Published by the Company of Biologists Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_204770.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-20

stage 20

Spinal Cord

Entry 11

GEISHA Id	FOXA2.Anderson.2021	Data Source	Direct Submission
Complete cDNA Template Probe	show GCGCTGCCCCGCCTGGTGCCCGGGCAGGTACCTGAGGCAGAGCCGCGGGAAGGCATGAGGCAGAGCCGGGCCAGGGCCG GGAGGAAGGCGGCCTGAGGCCGAGCAGCCGCGGGGAGAAAGTATGCTGGGAGCGGTGAAAATGGAAGGCCATGAGCACA CGGACTGGAGCAACTACTACGGGGAGCCCGAGAGCTACTCCTCGGTGAGCAACATGAACGCGGGGCTGGGCATGAACAG CATGAACACGTACATGACCATGTCGGCCATGAGCACCACGGCCAACATGACGGCAGCCACCTCCATGAACATGTCCTAC GCCAACACAGGCATGAGCCCTTCACTCGCCGGCATGTCCCCAGGTGCGGGGGCCATGGCCGGCATGGGCTCGGCCGGCG TGGCGGGGATGGGTGCCCACCTGAGCCCCAGCATGAGCCCCATGGGAGGCCAAGCGGGCTCTATGAACGCCCTGGCCCC CTACACCAACATGAATTCCATGAGCCCCATCTATGGGCAATCCAACCTCAACCGCTCGCGGGACCCCAAGACGTACCGG CGGAGCTACACGCACGCCAAGCCCCCCTACTCCTACATCTCGCTCATCACCATGGCCATCCAGCAGTCGCCCAACAAGA TGCTGACGCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGCCAGAACCAGCAGCGCTGGCAGAA CAGCATCCGCCACTCGCTTTCCTTCAATGACTGCTTCCTCAAGGTGCCCCGCTCCCCAGACAAGCCGGGCAAAGGCTCC TTCTGGACGCTGCACCCCGACTCGGGCAACATGTTTGAGAACGGCTGCTACCTGCGGCGCCAGAAGCGCTTCAAGTGTG AGAAGCAGCTGGCGACCAAGGACGGCGGCGGGGGCAAGAAGGGGCCGGGGCAGCCCCCCAGCCAGCCCCTGGGGGAGGG CAGCTCCTCGGGGGGCTCCGAGGGCTCGGCAGGAGCTGAGTCACCAGCGAGTGCCTCGCCGTGCCGGGACCACAAGCGT GCCCTGGCCGAGCTGAAGGGTGCGGCGGCGGGGCCGAGTCCCGGGGAGCCGTCGGCGGCCTCTCCAGCGCAGCACCTGC TGGCCCCGCCGCACGCCGGGCTGCCCCACGACGCCCACCTCAAGCCCGAGCACCACTACGCCTTCAACCACCCCTTCTC CATCAACAACCTGATGTCGTCCTCCGAGCAGCAGCACCACCACCACCCGCACCACCACCACCACCCCTCGCACAAAATG GACCTCAAGGCCTACGAGCAGGTGATGCACTACTCGGGCTACGCCTCGCCCGTGCCCGCCAGCCTGGCCATGGGGCCCG TAACGAACAAAAACCCATTGGAGTCCTCCCCTTTGGCCGGAGAGACTTCTTACTACCAAGGTGTGTATTCCCGGCCCAT CATGAACTCCTCCTAAAGGCAAAGGGGGAAAAACCCCCCCACCCCGCACGGTCCCCTAGCAGCGTGATGGACTGTGAGC GCTGCCGAGCACGGTACCTACCCATATAGCTATCTATCTATTTTTTGTTTTGTTCCCTGCTGGCTCCAGGTGGTTGCAT CCACATGGGGAGCAGCGGGCGCACCTCGCCTGGCACCCCCTAACCCTGACCCTGCGGTCCCCTCGGTTTGCCTTGCGGG GTGGTTCGGTTTGGTGGGAGGGGGGTGTAGGGGAGAGGGATTTTTCTGTTGGGGCTGGTTTCTTATTTTTTTCGTTGTC GTTGTCGCAAGGAGGTCTAAATATATCTATTTTTTTGTGTGGAAGTGATGGGGAAAAGAGAAAAACAAACAGACAAAAA TGGACGCAAAGAAAACGAAGCCCTCGTTCGGTGGGAAATCCGTGCGAGTTTGGGATCTGAGGATGGAAAGTCTTGTAAA ATTGTACAAAGGGGGAGAAAAAAAAAAACAAAAGAAAACGGGGGAAAAAAAAAGCGAAAAAAAAAAAAGTCAGGATAGA TCGTTGTAATTGAAACAAACGCTTGATGTTACCGGGAGAGTAAACTACTTGGATCTGATTAATTTATCGTTTCTGTATG CTTTATTTATGGCTTATAAATGTGTATTCTGGTAGCGACTAGCCAGGTTGCCACAGAACTCTATTAAAGTGTTATTGGC GGTTTT
Comments	Images provided by Claire Anderson. As insufficient information was provided to verify sequence used to synthesize probe, the sequence above was obtained from NCBI (accession number NM_204770.2). Reference paper: A Ruiz i Altaba, M Placzek, M Baldassare, J Dodd, T M Jessell. Early stages of notochord and floor plate development in the chick embryo defined by normal and induced expression of HNF-3 beta. Dev Biol. 1995 Aug;170(2):299-313. doi: 10.1006/dbio.1995.1216.