Geisha

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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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HOXC8
homeobox C8

Gene

Official Symbol	HOXC8
Official Full Name	homeobox C8
CGNC ID	49437
also known as	HOXC-8, homeobox protein Hox-C8\|homeo box C8
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204893
Polypeptide	NP_990224
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.413

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function	sequence-specific DNA binding transcription factor activity, more...
Biological Process	multicellular organismal development, transcription, DNA-templated
Cellular Component	nucleus

Links to other databases

Entrez Gene	395711
Ensembl Gene
KEGG	gga:395711

Entry 1

GEISHA Id	HOXC8.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	ACCACGGAACCTCCAGCATC
Reverse cDNA Template Primer	AATTAACCCTCACTAAAGGGGGGAAGCAAAAAGTAGGGG	cDNA Template Length	721
Forward cDNA Template Probe	show TGGGGGTNCCAATAGGAANTNCTATGACATAAAAAAGGCTACAACTGGTTTGACAGGTAGTTACTGCTGTGTGAGCCTC TCCATTCTATACTCTATAAAAGCAAATGACCGTCGTAAACATCTCGATTTATCATCTGCCATAAAACGAGACTTCAAGG GGCTGAGAACTCAGTCCTTGCTTTCTTCTTTTTCCTCCTCTTCTTTTTCCTCTTCCTCATTCCCTTCTTCTTCCGTTTT CTCCTCGTCTCTGGCCCCCGGCAGCTTATCTTTGTTGTTTTCTTTTTTCCACTTCATCCTCCTGTTCTGGAACCAGATC TTCACTTGTCTCTCGGTCAGTCCCAGGGCGTGAGAGACCTCAATCCGTCGCTTCCGTGTCAAATACGGGTTGAAGAGGA ACTCCTTTTCTAACTCCAGGGTCTGGTACCGGCTGTAAGTTTGTCTGCCACTGCGTCTTCCGGGAGCGTGAGGTCTCAT CCATGGGAACATGAGACTGGGAGACGAATTTTGATTTAAATGCCCTTGTCCCTCGCTAGAGTTACTGTTGGAAGACGAT TTACAGTCAGGATATTGTACAACAGTCGTCTCTTGCTGAGCACCATAAAGCGATTGCCTCGGCAGAGCTTCATATCCAT AGAATTTAGAAGCGTCTCCGTGACACGCCAAGGCGCACGGGTTTTGCTGGTATCCAGAGTTGGAGATGCTGGAGGTTCC GTGGT
Comments	No expression detected at or before stage 7.

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 9

stage 10

stage 11

Lateral Plate Mesoderm
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak
Spinal Cord
Unlabeled

Stage 9+ to 10-.
Stages 6-7.
Staining observed in neural tube may be due to trapping.

13-18

stage 13

stage 17

Lateral Plate Mesoderm
Limb Buds
Paraxial Mesoderm
Somatic Mesoderm
Somites
Spinal Cord
Tail

Staining observed in neural tube may be due to trapping.

19-24

stage 19

stage 21

stage 23

Leg
Limb Buds
Paraxial Mesoderm
Somatic Mesoderm
Somites
Spinal Cord
Tail
Wing

25-29

stage 25

stage 29

Leg
Somites
Spinal Cord
Surface Ectoderm
Tail
Wing

epidermis

Entry 2

GEISHA Id	HOXC8.Reid.2002	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGTTGCTGTCGGCTCTACCTACCGACAGATTACAAGAGAAGTCAAGAGCACCGGGAACGCACCGTGCGGGCAC CAGAGGCACCATGCAACGCCTTTGAGATCGCCTTTGAAATATATATATTAATAATAATTTTATAATAATAATAAAAAAG CCACCTTTTTTATATCTATATATATCTATCTCTCTATTTTAAAAAAAAAAAAAAGACAACAACAACAACAACAAAATAA ATAAATAAATAAATAATAAAAAAAAGCCGGACTGGTAAACAGAGAGAGAGAGAGAGAGAGAGAGAGAAGAACAGTCCGG GACCGTCAGCACTCATCCAGAATTAAAGCCGTCTTTTTAATTGTTTTTAATTGTTTTTTTTTTTAATTATTTTTTTCAA TTTTTAATTTTTTTTCAATTTTTATATATATATATGGTTTTTTTTTTTGCATGCGAGGGTCATGAGTTCCTACTTTGTA AATCCTCTCTTTTCCAAGTACAAAGGCGGCGAATCGCTGGAACCAACTTACTACGACTGCAGATTTCCACAAAGTGTTA GCAGGAGCCATGCTCTCGTGTATGGTCCGGGCACCACTGCTCCCACCTTCCAGCACCCATCACACCACGTCCAAGAGTT TTTCCACCACGGAACCTCCAGCATCTCCAACTCTGGATACCAGCAAAACCCGTGCGCCTTGGCGTGTCACGGAGACGCT TCTAAATTCTATGGATATGAAGCTCTGCCGAGGCAATCGCTTTATGGTGCTCAGCAAGAGACGACTGTTGTACAATATC CTGACTGTAAATCGTCTTCCAACAGTAACTCTAGCGAGGGACAAGGGCATTTAAATCAAAATTCGTCTCCCAGTCTCAT GTTCCCATGGATGAGACCTCACGCTCCCGGAAGACGCAGTGGCAGACAAACTTACAGCCGGTACCAGACCCTGGAGTTA GAAAAGGAGTTCCTCTTCAACCCGTATTTGACACGGAAGCGACGGATTGAGGTCTCTCACGCCCTGGGACTGACCGAGA GACAAGTGAAGATCTGGTTCCAGAACAGGAGGATGAAGTGGAAAAAAGAAAACAACAAAGATAAGCTGCCGGGGGCCAG AGACGAGGAGAAAACGGAAGAAGAAGGGAATGAGGAAGAGGAAAAAGAAGAGGAGGAAAAAGAAGAAAGCAAGGACTGA GTTCTCAGCCCTTGAAGTCTCGTTTTATGGCAGATGATAAATCGAGATGTTTACGACGGTCATTTGCTTTTATAGAGTA TAGAATGGAGAGGCTCACACAGCAGTAACTACCTGTCAAACAGTTGTAGCCTTTTTTATTGTCATAGAAACTTCCCCTA CTTTTTGCTTCCCCCCCCCCCCCCACTCTTTTTGTTTTGTTTTGTTTTGCTTTGCTTTGCTTTTTAATAGCGGCGTTTT TAGCTCCATAAATACATAGTTGGCTTCAGTGCAGCGACGGTATAAGCGGATGGG
Citation	Reid AI, Gaunt SJ. Colinearity and non-colinearity in the expression of Hox genes in developing chick skin. Int J Dev Biol. 2002 Mar
Copyright	Copyright © 2007 UBC Press
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204893).

Stage

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Location (click to highlight)

Comments (click to highlight)

25-30

stage 30

Dermis
Surface Ectoderm

31-44

stage 34

stage 44

Dermis
Spinal Cord
Surface Ectoderm
Vertebrae

Comparison of several genes

Entry 3

GEISHA Id	HOXC8.Hughes.2011	Data Source	Publication
Complete cDNA Template Probe	show GAATTCCGTTGCTGTCGGCTCTACCTACCGACAGATTACAAGAGAAGTCAAGAGCACCGGGAACGCACCGTGCGGGCAC CAGAGGCACCATGCAACGCCTTTGAGATCGCCTTTGAAATATATATATTAATAATAATTTTATAATAATAATAAAAAAG CCACCTTTTTTATATCTATATATATCTATCTCTCTATTTTAAAAAAAAAAAAAAGACAACAACAACAACAACAAAATAA ATAAATAAATAAATAATAAAAAAAAGCCGGACTGGTAAACAGAGAGAGAGAGAGAGAGAGAGAGAGAAGAACAGTCCGG GACCGTCAGCACTCATCCAGAATTAAAGCCGTCTTTTTAATTGTTTTTAATTGTTTTTTTTTTTAATTATTTTTTTCAA TTTTTAATTTTTTTTCAATTTTTATATATATATATGGTTTTTTTTTTTGCATGCGAGGGTCATGAGTTCCTACTTTGTA AATCCTCTCTTTTCCAAGTACAAAGGCGGCGAATCGCTGGAACCAACTTACTACGACTGCAGATTTCCACAAAGTGTTA GCAGGAGCCATGCTCTCGTGTATGGTCCGGGCACCACTGCTCCCACCTTCCAGCACCCATCACACCACGTCCAAGAGTT TTTCCACCACGGAACCTCCAGCATCTCCAACTCTGGATACCAGCAAAACCCGTGCGCCTTGGCGTGTCACGGAGACGCT TCTAAATTCTATGGATATGAAGCTCTGCCGAGGCAATCGCTTTATGGTGCTCAGCAAGAGACGACTGTTGTACAATATC CTGACTGTAAATCGTCTTCCAACAGTAACTCTAGCGAGGGACAAGGGCATTTAAATCAAAATTCGTCTCCCAGTCTCAT GTTCCCATGGATGAGACCTCACGCTCCCGGAAGACGCAGTGGCAGACAAACTTACAGCCGGTACCAGACCCTGGAGTTA GAAAAGGAGTTCCTCTTCAACCCGTATTTGACACGGAAGCGACGGATTGAGGTCTCTCACGCCCTGGGACTGACCGAGA GACAAGTGAAGATCTGGTTCCAGAACAGGAGGATGAAGTGGAAAAAAGAAAACAACAAAGATAAGCTGCCGGGGGCCAG AGACGAGGAGAAAACGGAAGAAGAAGGGAATGAGGAAGAGGAAAAAGAAGAGGAGGAAAAAGAAGAAAGCAAGGACTGA GTTCTCAGCCCTTGAAGTCTCGTTTTATGGCAGATGATAAATCGAGATGTTTACGACGGTCATTTGCTTTTATAGAGTA TAGAATGGAGAGGCTCACACAGCAGTAACTACCTGTCAAACAGTTGTAGCCTTTTTTATTGTCATAGAAACTTCCCCTA CTTTTTGCTTCCCCCCCCCCCCCCACTCTTTTTGTTTTGTTTTGTTTTGCTTTGCTTTGCTTTTTAATAGCGGCGTTTT TAGCTCCATAAATACATAGTTGGCTTCAGTGCAGCGACGGTATAAGCGGATGGG
Citation	Hughes MW, Wu P, Jiang TX, Lin SJ, Dong CY, Li A, Hsieh FJ, Widelitz RB, Chuong CM. In search of the Golden Fleece: unraveling principles of morphogenesis by studying the integrative biology of skin appendages Integr Biol (Camb). 2011 Apr;3(4):388-407. Epub 2011 Mar 24.
Copyright	© Royal Society of Chemistry 2011
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204893.1).

Stage

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Location (click to highlight)

Comments (click to highlight)

25-29

stage 26

stage 29

Feathers/Scutae
Surface Ectoderm

epidermis

Entry 4

GEISHA Id	Hoxc-8.Nelson.1996	Data Source	Publication
Complete cDNA Template Probe	show CTCTACCTACCGACAGATTACAAGAGAAGTCAAGAGCACCGGGAACGCACCGTGCGGGCACGCAGAGGCACCATGCAAC GCCTTTGAGATCGCCTTTGAAATATATATATTAATATTAATAATAATAATAATAATAAAAAAGCCACCTTTTTATATCT ATATATATCTATCTCTCTATTTAAAAAAAAAAAAAAAGACAACAACAACAACAACAAAATAAATAAATAAATAAATAAT AAAAAAAAGCCGGACTGGAAACAGAGAGAGAGAGAGAGAGAGGGAGAGAGGAAAACAGTCCGGGACCGTCAGCACTCAT CCAGAATTAAAGCCGTCTTTTTAATTGTTTTTTTTTTTTAATTATTTTTTTTTCAATTTTTTAAATTTTTTTTTTCAAT TTTTTTATTATTATTATTATTATTGTTTTTTTTTTTTTTTTTGCATGCGAGGGTCATGAGTTCCTACTTTGTAAATCCT CTCTTTTCCAAGTACAAAGGCGGCGAATCGCTGGAACCAACTTACTACGACTGCAGATTTCCACAAAGTGTTAGCAGGA GCCATGCTCTCGTGTATGGTCCGGGCACCACTGCTCCCACCTTCCAGCACCCATCACACCACGTCCAAGAGTTTTTCCA CCACGGAACCTCCAGCATCTCCAACTCTGGATACCAGCAAAACCCGTGCGCCTTGGCGTGTCACGGAGACGCTTCTAAA TTCTATGGATATGAAGCTCTGCCGAGGCAATCGCTTTATGGTGCTCAGCAAGAGACGACTGTTGTACAATATCCTGACT GTAAATCGTCTTCCAACAGTAACTCTAGCGAGGGACAAGGGCATTTAAATCAAAATTCGTCTCCCAGTCTCATGTTCCC ATGGATGAGACCTCACGCTCCCGGAAGACGCAGTGGCAGACAAACTTACAGCCGGTACCAGACCCTGGAGTTAGAAAAG GAGTTCCTCTTCAACCCGTATTTGACACGGAAGCGACGGATTGAGGTCTCTCACGCCCTGGGACTGACCGAGAGACAAG TGAAGATCTGGTTCCAGAACAGGAGGATGAAGTGGAAAAAAGAAAACAACAAAGATAAGCTGCCGGGGGCCAGAGACGA GGAGAAAACGGAAGAAGAAGGGAATGAGGAAGAGGAAAAAGAAGAGGAGGAAAAAGAAGAAAGCAAGGACTGAGTTCTC AGCCCCTTGAAGTCTCGTTTTATGGCAGATGATAAATCGAGATGTTTACGACGGTCATTTGCTTTTATAGAGTATAGAA TGGAGAGGCTCACACAGCAGTAACTACCTGTCAAACAGTTGTAGCCTTTTTTATTGTCATAGAAACTTCCCCTACTTTT TGCTCCCCCCCCCCCCCACTCTTTTTGTTTTGTTTTGTTTTGCTTTGCTTTGCTTTTTAATAGCGGCGTTTTTAGCTCC ATAAATACATAGTTGGCTTCAGTGCAGCGACGGTATAAGCGGATGGGGGGGGGGGAACGGTGCAGCAAAACCCGAATCC CCCCCCCCTCCCGTCCAAAGGCCGCAGCGCGTCCCCGGCTGCACAAAATGTATTTATTTGAACAGCTTTGACTTTGCCT TTTTCCGCTTGGTTTTGCCTCTCGCGAATGACCCCGAGGGAAGCGCGGGGGTGTCGGTGCTGCGGCCGTCTATGGGGGC CGATGGCCGTTCCTAAAAGCCGTTGATCACTCATTGAAGGCGAGATAAGTGGGGGCTCGGAGCCCACCTGGCAGCGCCC GCACCTCCTCCCCATTGCGTTCCCATTGGGTTCCCATTGAGTACCCATAGTGTTCCTATTGGGTTCCCGATGTGCTCCC ACTGGGTTCCCATTGCGTTCCCATTGGGTTCCCATCGCGTTCCCGCTGTGTTCCCACTGGGTTCCCATCGTGTTCCCAC TGGGTTCCCATTGCGTACCCATAGCGTTCCTATTGTGTTCCCATTGGGTTCCCATTGGGTTCCCATAGCGTTCCCATTG TGTTCCCATTGGGTTCCCATAGGGCTGAGTGTAGAAAATGCCTATATTTTTTTTCTGCTTGGGACTCAATAGGCCGAAA TTCGCCCCC
Citation	Nelson CE, Morgan BA, Burke AC, Laufer E, DiMambro E, Murtaugh LC, Gonzales E, Tessarollo L, Parada LF, Tabin C. Analysis of Hox gene expression in the chick limb bud. Development. 1996 May;122(5):1449-66.
Copyright	Copyright © 1996 by Company of Biologists
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI ( NM_204893).