GEISHA - A Chicken Embryo Gene Expression Database
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MCF2L
MCF.2 cell line derived transforming sequence like

Gene
Official SymbolMCF2L
Official Full NameMCF.2 cell line derived transforming sequence like
CGNC ID12632
also known asguanine nucleotide exchange factor DBS
gene typeprotein-coding
Genomic Map
 Ensembl
NCBI
UCSC
Gallus-Browse
Sequence Information
Genomic
RNANM_001252063
PolypeptideNP_001238992
Sequence Clusters/ESTsUnigene
Gene Expression
In Situ HybridizationGEISHA
EST ProfileGga.12805
Orthology
Entrez GeneEnsembl GeneGenetic PhenotypesMOD
Fruit Fly
Human23263ENSG00000126217MIM:609499
Mouse17207ENSMUSG00000031442All phenotypic alleles (1):Targeted, other(1) MGI:103263
Xenopus1004977306042030
Zebrafish100148439ENSDARG00000075859ZDB-GENE-090312-122, ZDB-GENE-081104-416ZFIN:ZDB-GENE-090312-122, ZFIN:ZDB-GENE-081104-416
Gene Ontology
Molecular Function
Biological Process
Cellular Component
Links to other databases
Entrez Gene418748
Ensembl GeneENSGALG00000016834
KEGGgga:418748

Entry 1

GEISHA IdMCF2L.UApcrData SourceGEISHA ISH Analysis
Forward cDNA Template PrimerGGTTAGCAGTAGAACAGGGAGACG
Reverse cDNA Template PrimerATTAACCCTCACTAAAGGGAGATTTTACAAAGGCTTCGGGTCcDNA Template Length879
Forward cDNA Template Probeshow
TTAGGTGCACTGGCTGAACAGGGACGTGTTTGCTTAGCTGCCAGTTTCTTAAGACTTACTTGTCTCTTTTGAAACATTT
CTTCCATGCTTTCTTGCTTCTGGAAAACCTTTTGTATATGCTCCTTTAGGTCCTCATTGAGGATGTGTGCATACTCTCT
GTAAACTTTATTCAACTCCTTAATTTTATTGCCTGCACCAGTGTCTAGGAATTTCTCAATCTCCTGTAATGCTGATTCT
GCACCATCCTGAGACTGACACTTATCTACAGGTTGTGAAGCCAGAAGATAAATTCCTTCATCGCACCACTTCATAGACT
TTTCCAGTAAGCCATGCAGTTCTAGAGATTTATTAAGAACATTCCTCCTCTGTTCCATCTCAGTAGTGAAGGCATCACA
TAGATGATGAAGCTCACTGCATTTTGGAATAATGGAATCCACAGCATAATGATTGCTCTGAATAAAAGCATCACCCTCT
GAAGCTAGCATTCTGGCTTTTGCTACAGTTTCACATGATTTTTC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 4
stage 5
stage 6
Area Pellucida
Extraembryonic Mesoderm
Hensen's Node
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak
 
7-12
stage 7
stage 10
stage 11
stage 12
Anterior Neuropore
Blood Islands
Extraembryonic Mesoderm
Head Mesenchyme
Hensen's Node
Intermediate Mesoderm
Lateral Plate Mesoderm
Neural Plate/Tube
Notochord
Paraxial Mesoderm
Primitive Streak
Somites
 
13-18
stage 13
stage 13
stage 16
stage 16
Blood Islands
Eye
Face Mesenchyme
Forebrain
Head Mesenchyme
Hindbrain
Intermediate Mesoderm
Lung
Midbrain
Myotome
Neural Plate/Tube
Notochord
Somites
 
19-24
stage 23
stage 23
stage 23
Forebrain
Gut
Head Mesenchyme
Midbrain
Somatic Mesoderm
Somites
Trunk Mesenchyme
 
25
stage 25
Eye
Forebrain
Head Mesenchyme
Midbrain
Notochord
Somites
Spinal Cord
Trunk Mesenchyme
 

Entry 2

GEISHA IdMCF2L.Anderson.2022Data SourceGEISHA ISH Analysis
Complete cDNA Template Probeshow
TAAAAGATCTGTATGTCAAAGGCAATCTGTAAATCAAGGTCATCGCCCTTGTCCAGGTCCAAATTTGTTGGAGATGGAT
TGATTGTCTTAATTTTCTTCCAATAAACAGTCCTTCAAGGAACAACATCAGAAACGTGAAAAAAATGGATGAGAGAAAA
GCTGATCTCACAAGTCTAGAAGGTTATGTCACCACAGTAGCACCAAAGTACCCCGAGAAAGGCAAAGGTTGGGCTAAAA
CGTCTCATTCTCTTGATGCATCTGAAGAAAATGATGGCTGGTCCAGTGCTGAGGAGCCGCTGAATTCATCGGATGCAGA
AGAAGAAGGAGGAGGAGGAGGAGAGAGAAATGAGATGAAGCTGACTCCTGGTAAATATACAGTTGTGTCTGACTACGAG
AAAGGAGTTTCTGAAGAATTTGCAGTGAAAGGTGGAGATCTAGTTCAACTGATTCGTGAAGGGGAGGAGGGACTTTGGT
ATGTGAAGAATCTGACCACTGGTAGAGAAGGTTGGATCCCAGCAAACAATCTGCTGATGCTCATAGGCAATTCAAAATC
AGCTCAATCTTTGAGCAGCTCTGAATCAGGCACTGCTTCCAGCACTTTGAGCACGTCATCAAGTTGCAGTGATAGCTGC
AATGCCAGCAGCACCAGCTTCTCAGACATTAAGGGCTAATGTCATATGTTCACCCCACCTCCACGGAAAGTAAATCTGG
AGTTTGAGCCCAACATCCAAAACTCGAGATGGCCATCAGAGAACTATCTGTTCTGAATTGCTGGTTTTTCAGGTGTTTT
CTCTGATTTTCAGAGATTCTCACAGAAGATCCTTATGCAAAAGTGCTTGAATG
CitationAnderson C, Khan MAF, Wong F, Solovieva T, Oliveira NMM, Baldock RA, Tickle C, Burt DW, Stern CD. A strategy to discover new organizers identifies a putative heart organizer. Nat Commun. 2016 Aug 25;7:12656. doi: 10.1038/ncomms12656.
CopyrightCopyright © 2016, The Author(s)
CommentsImages and probe sequence provided by Claire Anderson. The probe was synthesized from cDNA clone ChEST103i7. Related paper: Anderson C, Khan MAF, Wong F, Solovieva T, Oliveira NMM, Baldock RA, Tickle C, Burt DW, Stern CD. A strategy to discover new organizers identifies a putative heart organizer. Nat Commun. 2016 7:12656. doi: 10.1038/ncomms12656.
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 4
stage 4
stage 6
Extraembryonic Mesoderm
Hensen's Node
Notochord
Primitive Streak
 
7-12
stage 8
stage 12
Ear/Otic Placode
Forebrain
Hensen's Node
Hindbrain
Midbrain
Neural Plate/Tube
Notochord
Somites
 
19-24
stage 21
stage 24
Myotome
 
 Data from NCBI Unigene EST Profile
Unigene ID: Gga.12805

Mouse

EmageMGI:103263
EurexpressMGI:103263
MGI GXDMGI:103263

Frog

Xenbase6042030

Fruit Fly

BDGP

Zebra Finch

ZEBrA

Zebrafish

ZFINZDB-GENE-090312-122, ZDB-GENE-081104-416
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