The following probes were used in this study: MyoD, clone CMD9 1.5-kb full-length fragment (a gift from Professor Bruce Patterson); Pax-3, a 645-bp fragment corresponding to nucleotides 468-1,113 (a gift from Dr. Martin Goulding); Myf-5, a 1.1-kb fragment and Myogenin, a 1.2-kb fragment (both kind gifts from Professor Antony Graham); Pax-7 a 582-bp fragment (a kind gift from Dr. Susanne Dietrich).
According to authors , "MyoD, Pax3 and Myf5 anti-sense probes were a kind gift of Philippa Francis-West.." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001030363).
According to authors, "Total RNA preparation, RT-PCR and oligonucleotides for amplification of Myf5 cDNA were as described (Montarras et al., 1991)" As access to Montarras et al., 1991 paper was unavailable to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_001030363).
According to the authors, "The reported Myf-5 expression patterns were confirmed with two in situ hybridization probes. One from accession # X73250..." The sequences below was obtained from NCBI (NM_001030363 and X73250) using the primers provided in the paper.