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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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SPP1
secreted phosphoprotein 1

Gene

Official Symbol	SPP1
Official Full Name	secreted phosphoprotein 1
CGNC ID	8301
also known as	osteopontin\|SPP-1\|bone sialoprotein 1\|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), Osteopontin, Secreted phosphoprotein 1, SPP-1, OP
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204535
Polypeptide	NP_989866
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.3551

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function	cytokine activity
Biological Process	biomineral tissue development, cell adhesion, ossification
Cellular Component	extracellular matrix, extracellular space

Links to other databases

Entrez Gene	395210
Ensembl Gene	ENSGALG00000010926
KEGG	gga:395210

Entry 1

GEISHA Id	Osteopontin.Holleville.2003	Data Source	Publication
Complete cDNA Template Probe	show GGAGAAAGCCAGAGCCTCACTCAGCCCGCAGTAGGAGTTGCTGCTGGGATTGCCGGAGCCCCTTTGAGCAGCTGACTTC TCCAGCACAGGAAAGGTAAAACATCACAATGAAGCTGGCATTTCTTTGCTTGTGCTTTATCAGCATTGCTGCTGCATGG CCAGTGAGCAAATCCAGGCAGCATGCCATTTCTGCCAGCTCTGAAGAAAAATACGACCCCAGGAGCCACCACACACACA GGTACCACCAGGATCACGTGGATTCTCAGTCTCAGGAGCACCTGCAGCAGACACAGAATGACCTGGCTTCACTTCAGCA GACTCATTACTCTTCAGAAGAAAATGCTGATGTCCCAGAACAGCCGGACTTTCCTGACATTCCTAGCAAGAGCCAAGAG GCCGTGGATGATGATGATGATGATGACAATGATTCCAATGACACTGACGAGTCTGATGAAGTCGTCACAGACTTCCCCA CAGAGGCACCCGTGACACCTTTCAACCGTGGGGACAACGCAGGCCGGGGTGACAGTGTGGCATATGGCTTCAGAGCCAA AGCCCACGTGGTGAAGGCGAGCAAGCTCCGCAAAGCTGCCAGGAAGCTCATTGAGGATGACGCCACCGCGGAGGTTGGG GACAGCCAGCTAGCGGGCCTCTGGTTGCCCAAGGAGAGCCGCGAACAGGACAGCCGTGAGCTGGCCCAACATCAGAGCG TAGAGAACGACAGCCGGCCAAGATTTGACAGCCCTGAGGTGGACGGAGGAGACAGCAAGGCCAGTGCTGGGGTGGACAG CAGAGAGAGCCTGGCTAGCGCGTCCGCCGTCGACGCCAGCAACCAGACGCTAGAGAGCGCTGAGGATGCCGAAGATCGC CACAGCATTGAAAACAACGAGGTCACCCGTTAAGAGGAACCTGTGCATTGCTTCTTTCTTTTTTTTGTCATTGCTGTGC AAGCGCAGGGCTGGGGGGAGGGAGGAGAGCCATAGCGACGTGTGTTTGTAAATGTAGAGCGCGTGGCGGTGCTTAGGTC GAGGCGCCGTGGTGCCATTGAGCCGTACCGAGACGGGGCGAGCGCTGCCTGCGGGGATGCTGCGCGTCCCGCTGCTCCT GTGTATATTTTGTAACCGGATGGAAATAAAAACCACAAAAACCTCAAAAAAAAAAAAA
Citation	Holleville N, Quilhac A, Bontoux M, Monsoro-Burq AH. BMP signals regulate Dlx5 during early avian skull development. Dev Biol. 2003 May 1;257(1):177-89.
Copyright	Copyright © 2003 Elsevier Science (USA) All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204535).

Stage

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Location (click to highlight)

Comments (click to highlight)

31-42

stage 35

stage 38

stage 42

Head Mesenchyme

Osteoblasts

Entry 2

GEISHA Id	OP.Conen.2009	Data Source	Publication
Complete cDNA Template Probe	show GGAGAAAGCCAGAGCCTCACTCAGCCCGCAGTAGGAGTTGCTGCTGGGATTGCCGGAGCCCCTTTGAGCAGCTGACTTC TCCAGCACAGGAAAGGTAAAACATCACAATGAAGCTGGCATTTCTTTGCTTGTGCTTTATCAGCATTGCTGCTGCATGG CCAGTGAGCAAATCCAGGCAGCATGCCATTTCTGCCAGCTCTGAAGAAAAATACGACCCCAGGAGCCACCACACACACA GGTACCACCAGGATCACGTGGATTCTCAGTCTCAGGAGCACCTGCAGCAGACACAGAATGACCTGGCTTCACTTCAGCA GACTCATTACTCTTCAGAAGAAAATGCTGATGTCCCAGAACAGCCGGACTTTCCTGACATTCCTAGCAAGAGCCAAGAG GCCGTGGATGATGATGATGATGATGACAATGATTCCAATGACACTGACGAGTCTGATGAAGTCGTCACAGACTTCCCCA CAGAGGCACCCGTGACACCTTTCAACCGTGGGGACAACGCAGGCCGGGGTGACAGTGTGGCATATGGCTTCAGAGCCAA AGCCCACGTGGTGAAGGCGAGCAAGCTCCGCAAAGCTGCCAGGAAGCTCATTGAGGATGACGCCACCGCGGAGGTTGGG GACAGCCAGCTAGCGGGCCTCTGGTTGCCCAAGGAGAGCCGCGAACAGGACAGCCGTGAGCTGGCCCAACATCAGAGCG TAGAGAACGACAGCCGGCCAAGATTTGACAGCCCTGAGGTGGACGGAGGAGACAGCAAGGCCAGTGCTGGGGTGGACAG CAGAGAGAGCCTGGCTAGCGCGTCCGCCGTCGACGCCAGCAACCAGACGCTAGAGAGCGCTGAGGATGCCGAAGATCGC CACAGCATTGAAAACAACGAGGTCACCCGTTAAGAGGAACCTGTGCATTGCTTCTTTCTTTTTTTTGTCATTGCTGTGC AAGCGCAGGGCTGGGGGGAGGGAGGAGAGCCATAGCGACGTGTGTTTGTAAATGTAGAGCGCGTGGCGGTGCTTAGGTC GAGGCGCCGTGGTGCCATTGAGCCGTACCGAGACGGGGCGAGCGCTGCCTGCGGGGATGCTGCGCGTCCCGCTGCTCCT GTGTATATTTTGTAACCGGATGGAAATAAAAACCACAAAAACCTCAAAAAAAAAAAAA
Citation	Conen KL, Nishimori S, Provot S, Kronenberg HM. The transcriptional cofactor Lbh regulates angiogenesis and endochondral bone formation during fetal bone development. Dev Biol. 2009 Sep 15;333(2):348-58. Epub 2009 Jul 14.
Copyright	Copyright © 2009 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204535.4).

Stage

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Location (click to highlight)

Comments (click to highlight)

31-36

stage 36

Wing Bones

Entry 3

GEISHA Id	OPN.Kimura.2011	Data Source	Publication
Complete cDNA Template Probe	show CTCCAGCACAGGAAAGGTAAAACATCACAATGAAGCTGGCATTTCTTTGCTTGTGCTTTATCAGCATTGCTGCTGCATG GCCAGTGAGCAAATCCAGGCAGCATGCCATTTCTGCCAGCTCTGAAGAAAAATACGACCCCAGGAGCCACCACACACAC AGGTACCACCAGGATCACGTGGATTCTCAGTCTCAGGAGCACCTGCAGCAGACACAGAATGACCTGGCTTCACTTCAGC AGACTCATTACTCTTCAGAAGAAAATGCTGATGTCCCAGAACAGCCGGACTTTCCTGACATTCCTAGCAAGAGCCAAGA GGCCGTGGATGATGATGATGATGATGACAATGATTCCAATGACACTGACGAGTCTGATGAAGTCGTCACAGACTTCCCC ACAGAGGCACCCGTGACACCTTTCAACCGTGGGGACAACGCAGGCCGGGGTGACAGTGTGGCATATGGCTTCAGAGCCA AAGCCCACGTGGTGAAGGCGAGCAAGCTCCGCAAAGCTGCCAGGAAGCTCATTGAGGATGACGCCACCGCGGAGGTTGG GGACAGCCAGCTAGCGGGCCTCTGGTTGCCCAAGGAGAGCCGCGAACAGGACAGCCGTGAGCTGGCCCAACATCAGAGC GTAGAGAACGACAGCCGGCCAAGATTTGACAGCCCTGAGGTGGACGGAGGAGACAGCAAGGCCAGTGCTGGGGTGGACA GCAGAGAGAGCCTGGCTAGCGCGTCCGCCGTCGACGCCAGCAACCAGACGCTAGAGAGCGCTGAGGATGCCGAAGATCG CCACAGCATTGAAAACAACGAGGTCACCCGTTAAGAGGAACCTGTGCATTGCTTCTTTCTTTTTTTTGTCATTGCTGTG CAAGCGCAGGGCTGGGGGGAGGGAGGAGAGCCATAGCGACGTGTGTTTGTAAATGTAGAGCGCGTGGCGGTGCTTAGGT CGAGGCGCCGTGGTGCCATTGAGCCGTACCGAGAC
Citation	Kimura W, Alev C, Sheng G, Jakt M, Yasugi S, Fukuda K. Identification of region-specific genes in the early chicken endoderm. Gene Expr Patterns. 2011 Mar-Apr;11(3-4):171-80. Epub 2010 Nov 23.
Copyright	Copyright © 2010 Elsevier B.V. All rights reserved.
Comments	The cDNA template used to generate the probe was obtained by PCR as described. F, 5′-CTCCAGCACAGGAAAGGTAA-3′, R, 5′-GTCTCGGTACGGCTCAATGG-3′.