According to the authors, "Digoxigenin
(DIG)- and fluorescein-labeled riboprobes were synthesized from chick cDNA plasmids..." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_204784).
"(inner primers: 5'CCATGGAGCCGAGCCCGCAATTTTTC;
Outer primers: 5¢CTTTCTGAAAGTGGGGGACG; 3¢:
"Authors indicated the probe could be found in another publication (see text). The indicated probe sequence is included here"
Text from citation in paper: We have isolated the full coding sequence of the chick Wnt-11 by RTPCR, using 2- to 3-day-old chick total RNA as template. We chose
two sets of nested oligonucleotide primers within the published Wnt-
11 sequence (citation) to amplify the gene (inner primers: 5′CCATGGAGCCGAGCCCGCAATTTTTC; 3′GAATTCATTTGCAGACGTATCTCTC. Outer primers: 5′CTTTCTGAAAGTGGGGGACG; 3′:
In situ probes were generated from a 1-kb chicken Wnt11 clone encompassing the entire coding region