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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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WT1
Wilms tumor 1

The human orthologue of this gene is associated
with the following human diseases:

Gene

Official Symbol	WT1
Official Full Name	Wilms tumor 1
BirdBase ID	BB-GG19623
CGNC ID	9187
also known as	CWT1, Chick Wilm's tumour protein, Wilms tumor 1 , wt1
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_205216
Polypeptide	NP_990547
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.42868

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	7490	ENSG00000184937	MIM:194080	MIM:607102
Mouse	22431	ENSMUSG00000016458	All phenotypic alleles (17):Targeted, knock-out(6) Targeted, other(11)	MGI:98968
Xenopus	100216184, 373588, 378664			484998
Zebrafish	30245, 568416	ENSDARG00000031420, ENSDARG00000007990	ZDB-GENE-980526-558, ZDB-GENE-050420-319	ZFIN:ZDB-GENE-980526-558, ZFIN:ZDB-GENE-050420-319

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	396139
Ensembl Gene
KEGG	gga:396139

Entry 1

GEISHA Id	WT1.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	AGAGCCAGCAAGCCATTCG
Reverse cDNA Template Primer	ATTAACCCTCACTAAAGGTTTGGTGCCGTTTCAGTTGG	cDNA Template Length	663
Forward cDNA Template Probe	show GAAGAAAAANCTTCGTTCACAGTCCTTAAAATCACACTGGATATGNGTTTTTCACCAGCTGCTGCTTTCTGCTGGTGCA TCTGTAGATGGGACAGCTTGAAGTATCGCATTGCTTGCAGCCGGGGTAGGCACACATGAAGGGGCGTTTTTCATTTGTC TCACTTGCTGATCGGACAATAGTCGGAGCTACTCCTGGCACTCGTCGGACATCTTGTATGCCTCTAAAGACTCCATGGG TGTGTATTCTGTATTGGGCCCCACAGCTGTATAACATGGGAGCACTGTGGTTCTCATTTTCATATCCTGTCGTATGGCC CTTCAGCGTGGATCCCAGGTTCATTTGGTTCCATGTCATGCATTCCAGCTGTGACGTCATTTGGTACAAATTGTCACTG TTGTAGGGGGTCCGCAGGAGCAGGACCTGGCTGCCCGTGCAGCTGTCCGTGGGGGTGTGACAGCCGTACACCGGAGGGG GCACCGAGTACTGCTGGTCCCCTAGAGATGGCTGCTGGCTCATGGGGTCTTCGTGTTTGAAAGAGTGGTTCGTAAACTG CGCGGCGTGGTGAGACGGCGTGTGGCCGTAGCTCGGAGTCCCGTCAAAGGCCACGGTGCCGTAACCCTGGTTGCNAATG GCTGNCNTGGCTCTAAA

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Early Mesoderm
Epiblast
Extraembryonic Mesoderm
Primitive Streak

7-12

stage 8

stage 9

stage 11

Extraembryonic Mesoderm
Forebrain
Foregut
Intermediate Mesoderm
Neural Plate/Tube
Notochord
Sinus Venosus

13-18

stage 13

stage 15

Intermediate Mesoderm
Neural Plate/Tube
Proepicardium
Somatic Mesoderm

19-23

stage 20

stage 22

stage 23

Allantois
Ear/Otic Placode
Eye
Forebrain
Foregut
Gut
Heart
Hindbrain
Lung
Mesonephros
Midbrain
Nasal Placode/Nerve
Nephric Ducts
Notochord
Proepicardium
Sclerotome

Entry 2

GEISHA Id	Wt1.Schlueter.2006	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show ATGGGGTCCGACGTCCGGGACCTGAACGCGCTGCTGCCCTCCGTGCCCTCCCTACCGGGCAACAGCAACTGCGCCATGC CGGTGAGCAGCGCGGCGCAGTGGGCTCCCGTCCTGGACTTCCCCCCGGGGGCCTCCTACGGCTCGCTGGGGCCGCACTC CTTCATCAAGCAGGAACCCAGCTGGAACGGGTCGGACCCGCACGAGGAGCAGTACCTGAGCGCTTTCACCGTCCACTTC TCCGGGCAGTTCACGGGCACGGCCGGGGCCTGCCGCTACGGGCCCTTCGGCGCCCCGCCGCCCAGCCAGCCGCCCTCCG GCCAGGCCAGGATGTTCCCCCAACGGCCCTACCTGCCCAACTGCCTGGAGAGCCAGCAAGCCATTCGCAACCAGGGTTA CGGCACCGTGGCCTTTGACGGGACTCCGAGCTACGGCCACACGCCGTCTCACCACGCCGCGCAGTTTCAGAACCACTCT TTCAAACACGAAGACCCCATGAGCCAGCAGCCATCTCTAGGGGACCAGCAGTACTCGGTGCCCCCTCCGGTGTACGGCT GTCACACCCCCACGGACACGTGCACGGGCAGCCAGGCCCTGCTCCTGCGGACCCCCTACAACAGTGACAATTTGTACCA AATGACGTCACGACTGGAATGCATGACATGGAACCAAATGAACCTGGGATCCACGCTGAAGGGCCATACGACAGGATAT GAAAATGAGAACCACAGTGCTCCCATGTTATACAGCTGTGGGGCCCAATACAGAATACACACCCATGGAGTCTTTAGAG GCATACAAGATGTCCGACGAGTGCCAGGAGTAGCTCCGACTATTGTCCGATCAGCAAGTGAGACAAATGAAAAACGCCC CTTCATGTGTGCCTACCCCGGCTGCAACAAGCGATACTTCAAGCTGTCCCATCTACAGATGCACAGCAGAAAGCACACT GGTGAAAAACCATATCAGTGTGATTTTAAGGACTGTGAACGAAGATTTTCTCGTTCAGACCAACTGAAACGGCACCAAA GACGACACACAGGTGTGAAACCCTTCCAATGTAAAACCTGTCAGAGAAAGTTCTCCAGATCTGATCATCTGAAGACTCA TACCAGGACTCATACAGGTAAAACAAGTGAAAAGCCTTTCAGTTGCCGATGGCCCAGCTGTCAAAAAAAATTTGCCAGG TCTGATGAATTAGTCCGTCACCACAACATGCACCAGAGGAACATGACCAAACTCCAGCTGGCCCTTTAG
Comments	According to the authors, "A 1.3 kb Wt1 cDNA probe was kindly provided by T. Kudo, Ibaraki, Japan." As insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #NM 205216).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 13

stage 15

Intermediate Mesoderm
Proepicardium

19-22

stage 22

Atria
Ectoderm
Proepicardium
Sinus Venosus

epicardial and pericardial mesothelium

Entry 3

GEISHA Id	Wt1.Ishii.2007	Data Source	Publication
Complete cDNA Template Probe	show `ACAATTTGTACCAAATGACGTCACGACTGGAATGCATGACATGGAACCAAATGAACCTGGGATCCACGCTGAAGGGCCA TACGACAGGATATGAAAATGAGAACCACAGTGCTCCCATGTTATACAGCTGTGGGGCCCAATACAGAATACACACCCAT GGAGTCTTTAGAGGCATACAAGATGTCCGACGAGTGCCAGGAGTAGCTCCGACTATTGTCCGATCAGCAAGTGAGACAA ATGAAAAACGCCCCTTCATGTGTGCCTACCCCGGCTGCAACAAGCGATACTTCAAGCTGTCCCATC`
Citation	Ishii Y, Langberg JD, Hurtado R, Lee S, Mikawa T. Induction of proepicardial marker gene expression by the liver bud. Development. 2007 Oct;134(20):3627-37. Epub 2007 Sep 12.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. Permission granted by T. Mikawa.
Comments	Sequence below was obtained from NCBI (NM_205216) using the primers given in the publication: chick Wt1 (cWt1) forward primer, 5'-ACAATTTGTACCAAATGACGTCACAA-3' and reverse primer 5'-GATGGGACAGCTTGAAGTATCG-3'.