EphrinA2 transcripts are present throughout the mesoderm (as determined from sectioned embryos, not shown) during stages 4-7; during this period, low levels of transcripts are also present in the primitive streak (Fig. 1A-C). Between stages 7 and 12, ephrinA2 is strongly expressed throughout the neurogenic ectoderm, particularly in the posterior half of the mesencephalon (the prospective midbrain). After stage 7, ephrinA2 expression continues in the head mesenchyme and cardiogenic mesoderm but is down-regulated in the mesoderm posterior to the heart-forming region.
Authors said The cELF-1 cDNA fragment used to generate the probe is 700 bp in length and encodes the first 100 amino acids of the cELF-1 protein and 100 bp of the 5' untranslated region. The sequence listed above is NCBI Acc# NM_204983.