Authors indicated ""A 1,200-bp cMyoR cDNA fragment was generated using 5AGA GGG GAA GCA GTC GCA G 3 and 5ACA GCA TTC GGA TTA GTG AAG 3 primers." The probe listed begins with the first primer, but only 879 bases 3' of that are currently associated with this transcript in ensembl. These are listed here as probe sequence.
Gene specific primers were designed on the basis of the sequences available on the NCBI, the Ensembl and chick EST databases (http://www.ncbi.nlm.nih.gov/, http://www.ensembl.org/index.html, http://www.chick.umist.ac.uk), with MyoR forward: CGCGAATTCACTTGGCCATTTGTGGTCTC; MyoR reverse: CGCTCTAGAGTAAACCCGACCCGTGAATA, Located primers in NCBI Accession # XM_418293.
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As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # XM_418293.3).