Geisha

Early Mesoderm
Ectoderm
Epiblast
Head Process
Hensen's Node
Notochord
Primitive Streak

4-6

stage 4

stage 5

stage 6

Prechordal Plate

7-12

stage 8

stage 10

Central Nervous System
Neural Plate/Tube
Notochord
Spinal Cord

Floor Plate
Floor plate
Rostral
Rostral Floor Plate
floor plate

13-18

stage 13

stage 15

stage 16

stage 17

Hindbrain
Isthmus/MHB
Midbrain
Notochord

Floor Plate
Floor plate
Rostral

19-24

stage 22

stage 24

Isthmus/MHB
Spinal Cord

Floor Plate
Rostral

25-30

stage 26

stage 27

stage 30

Hindbrain
Isthmus/MHB
Midbrain
Neural Plate/Tube

Floor Plate
Rostral

Entry 6

GEISHA Id	Shh.Gimeno.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Gimeno L, Martinez S. Expression of chick Fgf19 and mouse Fgf15 orthologs is regulated in the developing brain by Fgf8 and Shh. Dev Dyn. 2007 Aug;236(8):2285-97.
Copyright	Copyright © 2007 Wiley-Liss, Inc.
Comments	According to the authors, "Digoxigenin and fluorescein RNA antisense probes for chick SHH gene was prepared according to the instructions of the manufacturer (Roche)." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (NM_204821).

Stage

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19-24

stage 19

stage 20

stage 22

stage 24

Diencephalon
Isthmus/MHB
Midbrain
Neural Plate/Tube

basal plates

25-26

stage 26

Diencephalon
Midbrain
Neural Plate/Tube

basal plates

Entry 7

GEISHA Id	Shh.Shin.2006	Data Source	Publication
Complete cDNA Template Probe	show GAATTCGCGGCCGCATCAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGC GACCTAAAGGCTGCCATTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACAT AGGAGGAGAGAGAGCTCTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAAT GCTGCTGTTGACAAGAATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGC AGGGGCATTGGAAAAAGGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGA AGACCCTAGGGGCCAGTGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTA CAACCCTGACATTATTTTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTG AATGCCCTGGCGATCTCGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCC ATCACTCCGAGGAATCGCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGG AATGCTGGCCCGCCTCGCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTC AAAGCAGAAAACTCAGTGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCA CCAAGCTGGTGAAGGACCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTT CCTCACCTTCCTCGACCGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTG CTACTGACGGCGGCCCACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGG CGCTCTTCGCCAGCAACGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTC TGTCCACAGCGTCTCATTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAAC CGGGTGTTGGCCTCCTGCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTC AGGGGCTGCTGGCCGCCCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTC ACGGCTCCTCTACCGCATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGC TGAGAGTGGCTGGAGAGGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAAAAGCGGCCGCGAATTC
Citation	Shin M, Noji S, Neubüser A, Yasugi S. FGF10 is required for cell proliferation and gland formation in the stomach epithelium of the chicken embryo. Dev Biol. 2006 Jun 1;294(1):11-23. Epub 2006 Apr 17.
Copyright	Reprinted from Developmental Biology, 294, Masahiron Shin, Sumihare Noji, Annette Neubüser, and Sadao Yasugl, 11-23, Copyright 2006, with permission from Elsevier. Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	Shh was a gift from Dr. T. Nohno, who cloned it using PCR primers similar to Riddle et al. 1993. Their clone sequence is inserted here (Acc. # L28099).

Stage

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31-38

stage 32

stage 37

stage 38

Stomach

proventriculus

Entry 8

GEISHA Id	SHH.Franz-Odendaal.2008	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Franz-Odendaal TA. Toward understanding the development of scleral ossicles in the chicken, Gallus gallus. Dev Dyn. 2008 Nov
Copyright	Copyright © 2009 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204821.1); insert length is 1.7 kb.

Stage

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31-38

stage 35

stage 38

Diencephalon
Neural Plate/Tube
Notochord

Entry 9

GEISHA Id	Shh.Tomonari.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Tomonari S, Takagi A, Noji S, Ohuchi H. Expression pattern of the melanopsin-like (cOpn4m) and VA opsin-like genes in the developing chicken retina and neural tissues. Gene Expr Patterns. 2007 Aug;7(7):746-53. Epub 2007 Jun 13.
Copyright	"Reprinted from Gene Expression Patterns, Volume number 7, Tomonari S, Takagi A, Noji S, Ohuchi H, Expression pattern of the melanopsin-like (cOpn4m) and VA opsin-like genes in the developing chicken retina and neural tissues.,746-53, Copyright (2007), with permission from Elsevier". Copyright © (2007) Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821 ).

Stage

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13-14

stage 14

floor plate

Entry 10

GEISHA Id	Shh.Garcia-Calero.2006	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	García-Calero E, Garda AL, Marín F, Puelles L. Expression of Lrrn1 marks the prospective site of the zona limitans thalami in the early embryonic chicken diencephalon. Gene Expr Patterns. 2006 Oct;6(8):879-85. Epub 2006 Feb 28.
Copyright	Reprinted with permission from Elsevier. Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	According to the authors, "Digoxigenin-labeled RNA probes were prepared from plasmids kindly provided by J.L.R. Rubenstein (Shh)..." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (NM_204821).

Stage

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13-18

stage 14

stage 16

stage 17

stage 18

Diencephalon
Midbrain

Basal Plate
Zona Limitans Thalami

stage 25

Diencephalon

Zona Limitans Thalami

Entry 11

GEISHA Id	Shh.Ha.2003	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Ha AS, Riddle RD. cBlimp-1 expression in chick limb bud development. Gene Expr Patterns. 2003 Jun;3(3):297-300.
Copyright	Reprinted from Gene Expr Patterns, Volume 3, Issue 3, Alice S. Ha and Robert D. Riddle, cBlimp-1 expression in chick limb bud development, Pages 297-300, Copyright 2003, with permission from Elsevier. Copyright © 2003 Elsevier Science B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204821).

Stage

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19-20

stage 20

Leg Mesenchyme

posterior

Entry 12

GEISHA Id	Shh.Cheng.2004	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Cheng L, Alvares LE, Ahmed MU, El-Hanfy AS, Dietrich S. The epaxial-hypaxial subdivision of the avian somite. Dev Biol. 2004 Oct 15;274(2):348-69.
Copyright	Reprinted from Developmental Biology, Volume 274, Issue 2, Louise Cheng, Lúcia E. Alvares, Mohi U. Ahmend, Amira S. El-Hanfy, and Susanne Dietrich, The epaxial-hypaxial subdivision of the avian somite, Pages 348-369, Copyright 2004, with permission from Elsevier. Copyright © 2004 Elsevier B.V. All rights reserved.
Comments	Authors indicate they used the same probe as Johnson et al., 1994. No probe information is listed in that paper. As information provided is insufficient to verify exact sequence used to synthesize the probe, the sequence below was obtained from NCBI (Acc# NM_204821.1).

Stage

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stage 7

Notochord
Spinal Cord

Multiple labels

Entry 13

GEISHA Id	Shh.Lobjois.2004	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Lobjois V, Benazeraf B, Bertrand N, Medevielle F, Pituello F. Specific regulation of cyclins D1 and D2 by FGF and Shh signaling coordinates cell cycle progression, patterning, and differentiation during early steps of spinal cord development. Dev Biol. 2004 Sep 15;273(2):195-209.
Copyright	Reprinted from Developmental Biology, Volume 273, Issue 2, Valérie Lobjois, Bertrand Benazeraf, Nicolas Betrand, François Medevielle and Fabienne Pituello, Specific regulation of cyclins D1 and D2 by FGF and Shh signaling coordinates cell cycle progression, patterning, and differentiation during early steps of spinal cord development, Pages 195-209, Copyright 2004, with permission from Elsevier. Copyright © 2004 Elsevier B.V. All rights reserved.
Comments	Authors said: chicken Gli 1 cDNA (2.2 kb), Shh cDNA (1.6 kb), and Patched1 cDNA (802 bp), gifts from C. Tabin. As information provided is insufficient to verify exact sequence used to synthesize the probe, the sequence below was obtained from NCBI (Acc# NM_204821.1)

Stage

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7-10

stage 10

Notochord

Entry 14

GEISHA Id	Shh.Maden.2007	Data Source	Publication
Complete cDNA Template Probe	show `CCCACCAGTGGCCAGGCATTCTTTGCCAGCAATGTGAAGCCTGGCCAACGTGTNTATGTGCTGGGAGAGGGCGGGCGGC AGCTGCTACCTGCATCTGTCCACAGCGTCTCATTGCGGGAGGAGGCATCCGGTGCATACGCCCCGCTCACCGCCCAAGG CACCATCCTCATCAACCGGGTGCTGACCTCCTGCTACGCTGTTATCGAGGAGCACAGTTGGGCCCATTGGGCCTTTGCA CCATTCCGCTTGGCTCAGGGGCTGCTGGCTGCCCTNTGCCCAGATGGGGCCATCCCTTTTGCCGGCACCACCACCACCG GCATCCATTGGTACTCACGGCTCCTTTACCACATCGGCAGCTGGGTGCTGGATGGTGATGCACTGCACCCACTGGGCAT GGTGGCACCGGCCAGCTGAGAGCGGCTGGATAGGGGCAGAGACAGAGTGTGGGAGTGAGAGAGAGAAAAAAAAA`
Citation	Maden M, Blentic A, Reijntjes S, Seguin S, Gale E, Graham A. (2007). Retinoic acid is required for specification of the ventral eye field and for Rathke's pouch in the avian embryo. Int J Dev Biol. 2007;51(3):191-200.
Copyright	"Reprinted from Int. J. Dev. Biol., 51, Maden M, Blentic A, Reijntjes S, Seguin S, Gale E, Graham A, Retinoic acid is required for specification of the ventral eye field and for Rathke's pouch in the avian embryo, 191-200, Copyright (2007), with permission from Copyright © 2007 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #AF022882). Images and sequence given are quail, but map shown is chick.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 9

stage 10

Head Process
Neural Plate/Tube
Notochord

Floor plate
Quail

13-18

stage 18

Diencephalon
Hindbrain
Midbrain
Notochord

Floor plate
Quail

31-44

stage 44

Diencephalon
Forebrain
Hindbrain
Midbrain
Somites

Fig. 3. All images for comparison.
Quail

Entry 15

GEISHA Id	Shh.Bardot.2004	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Bardot B, Lecoin L, Fliniaux I, Huillard E, Marx M, Viallet JP. Drm/Gremlin, a BMP antagonist, defines the interbud region during feather development. Int J Dev Biol. 2004;48(2-3):149-56.
Copyright	"Reprinted from Int. J. Dev. Biol., 48, Bardot B, Lecoin L, Fliniaux I, Huillard E, Marx M, Viallet JP, Drm/Gremlin, a BMP antagonist, defines the interbud region during feather development, 149-56, Copyright (2004), with permission from Copyright © 2004 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 22

Feathers/Scutae
Wing

31-44

stage 44

Feathers/Scutae
Wing

comparison to other probes

Entry 16

GEISHA Id	Shh.Christ.2002	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Christ B, Brand-Saberi B. Limb muscle development. Int J Dev Biol. 2002;46(7):905-14.
Copyright	"Reprinted from Int. J. Dev. Biol., 46, Christ B, Brand-Saberi B, Limb muscle development, 905-14, Copyright (2002), with permission from Copyright © 2002 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 23

Limb Buds

31-44

stage 44

Limb Buds

Compare with other genes

Entry 17

GEISHA Id	Shh.Pearse.2001	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Pearse RV, Vogan KJ, Tabin CJ. Ptc1 and Ptc2 transcripts provide distinct readouts of Hedgehog signaling activity during chick embryogenesis. Dev Biol. 2001 Nov 1;239(1):15-29.
Copyright	Reprinted with permission from Elsevier. Copyright © 2001 Elsevier B.V. All rights reserved.
Comments	According to the authors, "Digoxigenin (Dig)-labeled antisense RNA probes for Shh were generated from linearized DNA templates as described previously (Riddle et al., 1993)." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

Head Process
Hensen's Node

FIG. 3. Comparison of Shh, Ptc1, and Ptc2 expression at stage 5+.
Left side of Hensen's node.

19-24

stage 19

stage 20

stage 21

stage 22

stage 23

stage 24

Endoderm
Gut
Limb Buds
Neural Plate/Tube
Notochord
Wing
Wing Mesenchyme

Endodermal lining of the gut.
Floor Plate
Zone of Polarizing Activity

25-30

stage 25

stage 30

Wing Mesenchyme

FIG. 2. Developmental overview of chick Ptc2 expression, showing whole-mount and section in situ hybridizations with probes directed against Ptc2, Ptc1, or Shh.
FIG. 4. Comparison of Shh, Ptc1, and Ptc2 expression during early limb bud development.

Entry 18

GEISHA Id	Shh.Agarwala.2005	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Agarwala S, Aglyamova GV, Marma AK, Fallon JF, Ragsdale CW. Differential susceptibility of midbrain and spinal cord patterning to floor plate defects in the talpid2 mutant. Dev Biol. 2005 Dec 1;288(1):206-20. Epub 2005 Oct 21.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	According to the authors, "Digoxigenin (DIG)- and fluorescein-labeled riboprobes were synthesized from chick cDNA plasmids..." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Midbrain
Neural Plate/Tube
Spinal Cord

Floor Plate
Lateral Floor Plate
Medial Floor Plate
Notochord
Rostral Floor Plate

19-24

stage 22

Midbrain
Neural Plate/Tube
Spinal Cord

Floor Plate
Notochord
Rostral Floor Plate

25-28

stage 26

stage 28

Midbrain
Neural Plate/Tube
Spinal Cord

Medial Floor Plate
Rostral Floor Plate

Entry 19

GEISHA Id	SHH.Scherz.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Scherz PJ, McGlinn E, Nissim S, Tabin CJ. Extended exposure to Sonic hedgehog is required for patterning the posterior digits of the vertebrate limb.Dev Biol. 2007 Aug 15;308(2):343-54. Epub 2007 May 31.
Copyright	Reprinted with permission from Elsevier. Copyright 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #NM_204821.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 21

stage 24

Leg

Entry 20

GEISHA Id	Shh.Vieira.2005	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Vieira C, Garda AL, Shimamura K, Martinez S. Thalamic development induced by Shh in the chick embryo. Dev Biol. 2005 Aug 15;284(2):351-63.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	According to the authors, "Digoxigenin and fluorescein-labeled RNA probes were prepared from plasmids kindly provided by J.L.R. Rubenstein." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 17

Forebrain
Midbrain

mesencephalic and diencephalic basal plates

19-24

stage 19

stage 23

Central Nervous System
Forebrain
Midbrain

mesencephalic and diencephalic basal plates
zona limitans intrathalamica

stage 25

Central Nervous System
Forebrain
Midbrain

mesencephalic and diencephalic basal plates
zona limitans intrathalamica
zona limitans intrathalamica.

Entry 21

GEISHA Id	Shh.Nissim.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Nissim S, Allard P, Bandyopadhyay A, Harfe BD, Tabin CJ. Characterization of a novel ectodermal signaling center regulating Tbx2 and Shh in the vertebrate limb. Dev Biol. 2007 Apr 1;304(1):9-21.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in the publication to verify the exact sequence used to synthesize the probe, the sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 22

stage 25

Entry 22

GEISHA Id	Shh.Liu.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Liu G, Moro A, Zhang JJ, Cheng W, Qiu W, Kim PC. The role of Shh transcription activator Gli2 in chick cloacal development. Dev Biol. 2007 Mar 15;303(2):448-60. Epub 2006 Dec 15
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 17

Hindgut
Midgut

19-24

stage 23

Hindgut
Leg
Notochord
Spinal Cord

cloacal endoderm
floor plate of neural tube, ZPA, cloaca

25-26

stage 26

Hindgut
Neural Plate/Tube
Notochord

floor plate, cloaca

Entry 23

GEISHA Id	Shh.Haworth.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Haworth KE, Wilson JM, Grevellec A, Cobourne MT, Healy C, Helms JA, Sharpe PT, Tucker AS. Sonic hedgehog in the pharyngeal endoderm controls arch pattern via regulation of Fgf8 in head ectoderm. Dev Biol. 2007 Mar 1;303(1):244-58.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Authors indicate Shh was linearised with Sal1 and transcribed with Sp6. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

stage 11

Head Process
Notochord
Oral Pharynx
Pharynx

Double label, SHH red, FGF8 purple

13-15

stage 15

Head Process
Notochord
Oral Pharynx
Pharyngeal Arches and Clefts
Pharynx

Entry 24

GEISHA Id	Shh.Zhang.2001	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Zhang XM, Yang XJ (2001). Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis. Dev Biol. 2001 May 15;233(2):271-90.
Copyright	Copyright © 2001 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

stage 11

stage 12

Eye
Forebrain
Foregut
Mesoderm

Optic Vesicles
Prechordal Mesoderm
Primitive Foregut
Ventral Forebrain

13-18

stage 13

stage 14

stage 18

Diencephalon
Forebrain

Ventral Diencephalon
Ventral Forebrain

19-24

stage 24

Head Process
Hensen's Node

Entry 25

GEISHA Id	Shh.Levin.1995	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Levin M, Johnson RL, Stern CD, Kuehn M, Tabin C (1995). A molecular pathway determining left-right asymmetry in chick embryogenesis. Cell. 1995 Sep 8;82(5):803-14.
Copyright	Copyright © 1995 Elsevier Inc. All Rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

7-12

stage 7

Eye
Feathers/Scutae
Surface Ectoderm

31-44

stage 44

Entry 26

GEISHA Id	Shh.Vasiliauskas.1999	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Vasiliauskas D, Hancock S, Stern CD. SWiP-1: novel SOCS box containing WD-protein regulated by signalling centres and by Shh during development. Mech Dev. 1999 Apr;82(1-2):79-94
Copyright	Copyright © 1999 Elsevier Science Ireland Ltd. All rights reserved
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

31-34

stage 34

Scleral Papillae

Entry 27

GEISHA Id	Shh.McKinnell.2004	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	McKinnell IW, Turmaine M, Patel K (2004). Sonic Hedgehog functions by localizing the region of proliferation in early developing feather buds. Dev Biol. 2004 Aug 1;272(1):76-88.
Copyright	Copyright © 2004 Published by Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-30

stage 30

Control

31-33

stage 31

stage 33

Head Process
Hensen's Node
Primitive Streak

Control

Entry 28

GEISHA Id	Shh.Bok.2005	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Bok J, Bronner-Fraser M, Wu DK. Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear. Development. 2005 May;132(9):2115-24. Epub 2005 Mar 23.
Copyright	Copyright for articles in Development are retained by the authors. Permission granted by MBF.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204821).

no images

Entry 29

GEISHA Id	Shh.Garcia-Castro.2000	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	García-Castro MI, Vielmetter E, Bronner-Fraser M. N-Cadherin, a cell adhesion molecule involved in establishment of embryonic left-right asymmetry. Science. 2000 May 12;288(5468):1047-51.
Copyright	Copyright © 2000, The American Association for the Advancement of Science
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

stage 4

Hensen's Node
Primitive Streak

Shh appears in chick embryo during gastrulation, prior to cell differentiation; therefore, no anatomy is available.

Entry 30

GEISHA Id	SHH.Quinlan.2009	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Quinlan R, Graf M, Mason I, Lumsden A, Kiecker C. Complex and dynamic patterns of Wnt pathway gene expression in the developing chick forebrain. Neural Dev. 2009 Sep 4;4:35.
Copyright	Copyright © 2009 Quinlan et al; licensee BioMed Central Ltd. [This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.]
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204821).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Forebrain
Hindbrain
Midbrain

SHH in red, double labeled

Entry 31

GEISHA Id	Shh.Garcia-Lopez.2009	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	García-López R, Soula C, Martínez S. Expression analysis of Sulf1 in the chick forebrain at early and late stages of development. Dev Dyn. 2009 Sep;238(9):2418-29.
Copyright	Copyright © 2009 John Wiley & Sons, Inc. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-26

stage 26

Neural Plate/Tube

Entry 32

GEISHA Id	SHH.Agarwala.2001	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Agarwala S, Sanders TA, Ragsdale CW. Sonic hedgehog control of size and shape in midbrain pattern formation. Science. 2001 Mar 16;291(5511):2147-50. Epub 2001 Mar 1.
Copyright	© 2011 American Association for the Advancement of Science. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 13

Forebrain
Hindbrain
Isthmus/MHB
Midbrain
Neural Plate/Tube

FP (hindbrain floor plate)
rFP (rostral floor plate)- ventral longitudinal zone of the neural tube

Entry 33

GEISHA Id	SHH.Agarwala.2002	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Agarwala S, Ragsdale CW. A role for midbrain arcs in nucleogenesis. Development. 2002 Dec;129(24):5779-88.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. Permission granted by Dr. Ragsdale.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821.1). Two-color whole-mount in situ hybridization was carried out on chick brain with digoxigenin- and ﬂuorescein-labeled riboprobes synthesized from chick cDNA plasmids for SHH

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Midbrain

Double label

25-26

stage 26

Ectoderm
Midbrain

Double label

Entry 34

GEISHA Id	SHH.Parkinson.2010	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Parkinson N, Collins MM, Dufresne L, Ryan AK. Expression patterns of hormones, signaling molecules, and transcription factors during adenohypophysis development in the chick embryo. Dev Dyn. 2010 Apr;239(4):1197-210.
Copyright	Copyright © 2010 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 21

Diencephalon
Pituitary (Rudiment)

25-30

stage 28

Diencephalon
Oral Pharynx

oe, oral epithelium

31-32

stage 32

Diencephalon
Pituitary (Rudiment)

Entry 35

GEISHA Id	SHH.Halilagic.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Halilagic A, Ribes V, Ghyselinck NB, Zile MH, Dollé P, Studer M. Retinoids control anterior and dorsal properties in the developing forebrain. Dev Bio 2007 Mar 1;303(1):244-58
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821.1). Images are quail, but sequence and map shown are chick.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 19

Diencephalon
Hindbrain
Midbrain
Telencephalon

hy, hypothalamus
quail embryo
zli, zona limitas intrathalamica

Entry 36

GEISHA Id	SHH.Mao.2009	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Mao J, McGlinn E, Huang P, Tabin CJ, McMahon AP. Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb. Dev Cell. 2009 Apr;16(4):600-6.
Copyright	don't have permission yet
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-17

stage 17

Limb Buds

ST 17/18
double-hybridization: SHH expression (black arrow), FGF8 expression (white arrow)

Entry 37

GEISHA Id	SHH.Luo.2006	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Luo J, Ju MJ, Redies C. Regionalized cadherin-7 expression by radial glia is regulated by Shh and Pax7 during chicken spinal cord development. Neuroscience. 2006 Nov 3
Copyright	© 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 23

Spinal Cord

25-28

stage 28

Spinal Cord

Entry 38

GEISHA Id	SHH.Dunn.2011	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Dunn IC, Paton IR, Clelland AK, Sebastian S, Johnson EJ, McTeir L, Windsor D, Sherman A, Sang H, Burt DW, Tickle C, Davey MG. The chicken polydactyly (Po) locus causes allelic imbalance and ectopic expression of Shh during limb development. Dev Dyn. 2011 May;240(5):1163-72.
Copyright	Copyright © 2011 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Limb Buds
Somites

25-26

stage 25

stage 26

Limb Buds
Somites
Unlabeled

Entry 39

GEISHA Id	SHH.Grevellec.2011	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Grevellec A, Graham A, Tucker AS. Shh signalling restricts the expression of Gcm2 and controls the position of the developing parathyroids. Dev Biol. 2011 May 15;353(2):194-205. Epub 2011 Feb 22.
Copyright	Copyright © 2011 Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 16

Foregut
Pharyngeal Arches and Clefts

19-21

stage 21

Endoderm
Pharyngeal Arches and Clefts

Entry 40

GEISHA Id	SHH.Chambers.2007	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Chambers D, Wilson L, Maden M, Lumsden A. RALDH-independent generation of retinoic acid during vertebrate embryogenesis by CYP1B1. Development. 2007 Apr;134(7):1369-83.
Copyright	© 2007
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-16

stage 16

Hindbrain
Neural Plate/Tube
Pharyngeal Arches and Clefts

control embryo
flat-mount of (Ba) embryo
normal embryo

Entry 41

GEISHA Id	SHH.Gros.2010	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Gros J, Feistel K, Viebahn C, Blum M, Tabin CJ. Cell movements at Hensens node establish Left/Right asymmetric gene expression in the chick. Science. 2009 May 15; 324(5929): 941944.
Copyright	© 2012 American Association for the Advancement of Science. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Head Process
Hensen's Node

4-5

stage 4

stage 5

Entry 42

GEISHA Id	SHH.Golden.1999	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Golden JA, Bracilovic A, McFadden KA, Beesley JS, Rubenstein JL, Grinspan JB. Ectopic bone morphogenetic proteins 5 and 4 in the chicken forebrain lead to cyclopia and holoprosencephaly. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2439-44.
Copyright	Copyright © 2012 National Academy of Sciences. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-23

stage 23

Forebrain
Head Process
Neural Plate/Tube
Notochord

floor plate

Entry 43

GEISHA Id	SHH.Kolpak.2005	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Kolpak A, Zhang J, Bao ZZ Sonic Hedgehog Has a Dual Effect on the Growth of Retinal Ganglion Axons Depending on Its Concentration. J Neurosci. 2005 March 30; 25(13): 34323441.
Copyright	Copyright © 2012 by the Society for Neuroscience
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204821.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

25-30

stage 27

stage 29

31-35

stage 31

stage 35

Diencephalon
Mesencephalon
Metencephalon
Telencephalon

Entry 44

GEISHA Id	SHH.Tapadia.2005	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Tapadia MD, Cordero DR, Helms JA. It's all in your head: new insights into craniofacial development and deformation. J Anat. 2005 November; 207(5): 461477.
Copyright	Copyright © 2005 The Author Journal compilation © 2005 Anatomical Society of Great Britain and Ireland
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

Neural Plate/Tube

13-18

stage 15

stage 17

19-24

stage 20

Diencephalon
Pharyngeal Arches and Clefts
Surface Ectoderm
Telencephalon

Red is SHH, facial ectoderm
facial ectoderm

25-30

stage 25

Diencephalon
Neural Plate/Tube
Pharyngeal Arches and Clefts
Surface Ectoderm
Telencephalon

facial ectoderm

31-41

stage 41

Entry 45

GEISHA Id	SHH.Brito.2006	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	José M. Brito, Marie-Aimée Teillet, Nicole M. Le Douarin. An early role for Sonic hedgehog from foregut endoderm in jaw development: Ensuring neural crest cell survival. Proc Natl Acad Sci U S A. 2006 August 1; 103(31): 1160711612.
Copyright	Copyright © 2006 by The National Academy of Sciences of the USA

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 8

stage 9

Endoderm
Foregut
Gut
Neural Plate/Tube

HH stage 8+
HH stage 9+
HH stage 9-

19-21

stage 21

Entry 46

GEISHA Id	SHH.Davey.2006	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Davey MG, Paton IR, Yin Y, Schmidt M, Bangs FK, Morrice DR, Smith TG, Buxton P, Stamataki D, Tanaka M, Münsterberg AE, Briscoe J, Tickle C, Burt DW. The chicken talpid3 gene encodesa novel protein essentialfor Hedgehog signaling. Genes Dev. 2006 May 15; 20(10): 13651377.
Copyright	Copyright © 2006, Cold Spring Harbor Laboratory Press
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-20

stage 19

stage 20

Limb Buds
Neural Plate/Tube
Notochord

Entry 47

GEISHA Id	SHH.Psychoyos.2008	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Psychoyos D, Finnell R. Double Whole Mount in situ Hybridization of Early Chick Embryos. J Vis Exp. 2008; (20): 904.
Copyright	Copyright © 2008, Journal of Visualized Experiments

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-8

stage 8

Neural Plate/Tube
Notochord

Entry 48

GEISHA Id	SHH.Fisher.2008	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Fisher ME, Clelland AK, Bain A, Baldock RA, Murphy P, Downie H, Tickle C, Davidson DR, Buckland RA. Integrating technologies for comparing 3D gene expression domains in the developing chick limb. Dev Biol. 2008 May 1;317(1):13-23. Epub 2008 Feb 7.
Copyright	Copyright © 2008 Elsevier Inc.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-23

stage 22

stage 23

Gut
Leg
Limb Buds
Neural Plate/Tube
Notochord
Wing

From Dublin
From Dundee
From Edinburgh
double label
double labeled
floor plate, ZPA

Entry 49

GEISHA Id	SHH.Lancman.2006	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Lancman JJ, Caruccio NC, Harfe BD, Pasquinelli AE, Schageman JJ, Pertsemlidis A, Fallon JF. Analysis of the regulation of lin-41 during chick and mouse limb development. Dev Dyn. 2005 Dec;234(4):948-60.
Copyright	Copyright © 2005 Wiley-Liss, Inc.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 20

stage 24

Leg

Entry 50

GEISHA Id	SHH.WangS.2004	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Wang S, Yu X, Zhang T, Zhang X, Zhang Z, Chen Y. Chick Pcl2 regulates the left-right asymmetry by repressing Shh expression in Hensen's node. Development. 2004 Sep;131(17):4381-91. Epub 2004 Aug 4.
Copyright	copyright 2004 to the authors. Published by the Company of Biologists Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204821.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Hensen's Node
Notochord

7-11

stage 11

Entry 51

GEISHA Id	SHH.Tao.2002	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Tao H, Yoshimoto Y, Yoshioka H, Nohno T, Noji S, Ohuchi H. FGF10 is a mesenchymally derived stimulator for epidermal development in the chick embryonic skin. Mech Dev. 2002 Aug;116(1-2):39-49.
Copyright	Copyright © 2012 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_204821.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 20

Feathers/Scutae
Leg

stage 31

Entry 52

GEISHA Id	SHH.Katsu.2012	Data Source	Publication
Complete cDNA Template Probe	show CAGACCCTTTTTGTCTCTCGATTGCTGTCTCCCGACCAAACTCCGATGTGTTCCGTTATCAGCGACCTAAAGGCTGCCA TTCCAGCACCTGTCTTGCTAGGGATCGGTGGATAGTATACGATCAGAAAGCAGACAAGGACATAGGAGGAGAGAGAGCT CTAGAGAGACAGCCAGGGATAGGCACAGAGAGCTGTGAAGTAATTGGATTCAATGGTCGAAATGCTGCTGTTGACAAGA ATTCTCTTGGTGGGCTTCATCTGCGCTCTTTTAGTCTCCTCTGGGCTGACTTGTGGACCAGGCAGGGGCATTGGAAAAA GGAGGCACCCCAAAAAGCTGACCCCGTTAGCCTATAAGCAGTTTATTCCCAATGTGGCAGAGAAGACCCTAGGGGCCAG TGGAAGATATGAAGGGAAGATCACAAGAAACTCCGAGAGATTTAAAGAACTAACCCCAAATTACAACCCTGACATTATT TTTAAGGATGAAGAGAACACGGGAGCTGACAGACTGATGACTCAGCGCTGCAAGGACAAGCTGAATGCCCTGGCGATCT CGGTGATGAACCAGTGGCCCGGGGTGAAGCTGCGGGTGACCGAGGGCTGGGACGAGGATGGCCATCACTCCGAGGAATC GCTGCACTACGAGGGTCGCGCCGTGGACATCACCACGTCGGATCGGGACCGCAGCAAGTACGGAATGCTGGCCCGCCTC GCCGTCGAGGCCGGCTTCGACTGGGTCTACTACGAGTCCAAGGCGCACATCCACTGCTCCGTCAAAGCAGAAAACTCAG TGGCAGCGAAATCAGGAGGCTGCTTCCCTGGCTCAGCCACAGTGCACCTGGAGCATGGAGGCACCAAGCTGGTGAAGGA CCTGAGCCCTGGGGACCGCGTGCTGGCTGCTGACGCGGACGGCCGGCTGCTCTACAGTGACTTCCTCACCTTCCTCGAC CGGATGGACAGCTCCCGAAAGCTCTTCTACGTCATCGAGACGCGGCAGCCCCGGGCCCGGCTGCTACTGACGGCGGCCC ACCTGCTCTTTGTGGCCCCCCAGCACAACCAGTCGGAGGCCACAGGGTCCACCAGTGGCCAGGCGCTCTTCGCCAGCAA CGTGAAGCCTGGCCAACGTGTCTATGTGCTGGGCGAGGGCGGGCAGCAGCTGCTGCCGGCGTCTGTCCACAGCGTCTCA TTGCGGGAGGAGGCGTCCGGAGCCTACGCCCCACTCACCGCCCAGGGCACCATCCTCATCAACCGGGTGTTGGCCTCCT GCTACGCCGTCATCGAGGAGCACAGTTGGGCCCATTGGGCCTTCGCACCATTCCGCTTGGCTCAGGGGCTGCTGGCCGC CCTCTGCCCAGATGGGGCCATCCCTACTGCCGCCACCACCACCACTGGCATCCATTGGTACTCACGGCTCCTCTACCGC ATCGGCAGCTGGGTGCTGGATGGTGACGCGCTGCATCCGCTGGGCATGGTGGCACCGGCCAGCTGAGAGTGGCTGGAGA GGGACAGAGACAGAGTGTGGGAGAGAGAAAAAAA
Citation	Katsu K, Tokumori D, Tatsumi N, Suzuki A, Yokouchi Y. BMP inhibition by DAN in Hensen's node is a critical step for the establishment of left-right asymmetry in the chick embryo. Dev Biol. 2012 Mar 1;363(1):15-26.
Copyright	Copyright © 2012. Published by Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_204821.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 6