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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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EN1
engrailed homeobox 1

Gene

Official Symbol	EN1
Official Full Name	engrailed homeobox 1
CGNC ID	9192
also known as	En-1, Gg-En-1, en-3, homeobox protein engrailed-1\|Engrailed-3\|engrailed protein\|homeobox protein en-1
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	XM_001234328
Polypeptide	XP_001234329
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish	100334734

Gene Ontology

Molecular Function	sequence-specific DNA binding, sequence-specific DNA binding transcription factor activity
Biological Process	multicellular organismal development
Cellular Component	nucleus

Links to other databases

Entrez Gene	771008
Ensembl Gene
KEGG	gga:771008

Entry 1

GEISHA Id	En-1.Prin.2004	Data Source	Publication
Complete cDNA Template Probe	show AGATCTGGTTCCAGAACAAGCGGGCCAAGATCAAGAAGGCGACGGGCATCAAGAACGGGCTGGCGCTGCACCTCATGGC CCAGGGACTGTACAACCACTCCACCACCACGGTGCAGGACAAAGAGGAGAGCGAGTGACGGCCCCGCGCCGACAGGTGC CACGAACACAGAGCTATTGTCTATCCATAGTATAAGGACTGCGAACGAGAGGAAAAAAAAAAAAAATATGAACGCTTAC AAAAAAAAAAAAAAAAAAAAAATCTCTATATAGCCAAACATATGACCCTGTATATATTTAATTTCAGGTAAGAAATATG TGTAGCGATCTCTATTTGCTGGACGGTTTCTCTCTCCCCTTCTCTCCCCCTCTCTCTTTTGTTCGTTTGTTGCTTTCCT TTCCTCCCCACGTCCGTCGCTCTTCGTTTTCGGTGTCCGCCTCCGCTCTTCCGTTCGATCCTTATTTGTGTGTGTGTGT GAATTTCTGTCTCCCCCCCCCCCCCTCTCTCTCTTTATTTTTATTTTAATTTTTTTTTTCCCGAGGAAAAAAAGACAAA AAAAAATAATAATAATAATAAATCTGATCCACAGACTGCGAGGCTGAACGGCCGCTCCAAGCCAAAGATTTTATTATGT TCAGAAATCTGTAGTCTGAAATAAAGTGTAGGCTGTGCTCAGCACGCGGCTGCCGCGGCTCCTTCTTTCGGCGCGTGTG TGTGTGTGGCACGGACACATCTGTGCACGCACAGCGCTGCTGCGATCCCTTTTGCTTTTTGGTGGATCAGCGCAGTGAA TTTTTGAAGCGACTCTCCTCTGTTTGTTTTAAAGCACTCCGAGCTC
Citation	Prin F, Logan C, D'Souza D, Ensini M, Dhouailly D. Dorsal versus ventral scales and the dorsoventral patterning of chick foot epidermis. Dev Dyn. 2004 Mar;229(3):564-78.
Copyright	Wiley-Liss, Inc. 2004
Comments	En-1, a previously isolated 800 bp BglIISstI chick genomic DNA fragment extending 3′ from the homeobox

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 21

Limb Buds

ventral ectoderm (ve) of the hindlimb

25-30

stage 30

Unlabeled Embryonic

31-40

stage 37

stage 39

stage 40

Leg Ectoderm
Surface Ectoderm

Entry 2

GEISHA Id	EN-1.Tomas.2011	Data Source	Publication
Complete cDNA Template Probe	show CGGCGGCAACTCGGCGGCAAGTCGGGGAGCACGGCAGCCCCGCCATCCTCCTCATGGGCTCCAATAATGGAGGAGCCGT GCTCAAGCCCGACTCGCAGCAGCCGCTGGTGTGGCCCGCCTGGGTGTACTGCACGCGGTACTCCGACCGGCCGTCCTCG GGCCCCCGCACCAGGAAGCTGAAGAAGAAGAAGACGGAGAAGGAGGACAAACGGCCGCGCACCGCCTTCACGGCCGAGC AGCTGCAGCGGCTGAAGGCGGAGTTCCAGGCGAACCGCTACATCACGGAGCAGCGGCGGCAGAGCCTGGCCCAGGAGCT GAGCCTCAACGAGTCCCAGATCAAGATCTGGTTCCAGAACAAGCGGGCCAAGATCAAGAAGGCGACGGGCATCAAGAAC GGGCTGGCGCTGCACCTCATGGCCCAGGGACTGTACAACCACTCCACCACCACGGTGCAGGACAAAGAGGAGAGCGAGT GACGGCCCCGCGCCGACAGGTGCCACGAACACAGAGCTATTGTCTATCCATAGTATAAGGACTGCGAACGAGAGGAAAA CAAACAACACAATTTTGACCGCTTACAAAAAAACAAACAACAAAAAAACCTCCTTTTTTTCCCAAATTTTTCCCCCTTG TTTTTTTTTCACTTTCCGGGTTAAAAAATTTTTTTTCCCACCCCTCAATGCCCGGACGGTTCTTCTTCTCCCTTTTTTT CC
Citation	Tomás AR, Certal AC, Rodríguez-León J. FLRT3 as a key player on chick limb development. Dev Biol. 2011 Jul 15;355(2):324-33.
Comments	Probe for en-1 was obtained from chicken EST ChEST92p12.

Stage

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Location (click to highlight)

Comments (click to highlight)

13-14

stage 14

Wing Mesenchyme

control embryo

Entry 3

GEISHA Id	EN1.Davey.2006	Data Source	Publication
Complete cDNA Template Probe	show ATGGGTCGTCTCCCACCCCCGGGGCACGGAGCCCCTAAGGCAGCACCCGGGGCACGGAGGCGGCGGGGAGCATCTTCCG ATGACGGCGCGACTCGGGCAAACTTCCCACGCCGGTGGCAGGGAGCCCCGCGCAGCCCCGCGGGTCGCGGTCCCGCGTG CGCCCCTACGGTGGGAGAAGAGCCCGGGGCCGCTCCCAGCCTCGAGGTGCCCCCGCCCGCGGGGCCCCGTCCCGGAGAG CGGCGGGGAGGGCGGAGGCCGGGAGGCGGCAGGCGTCGGCCCTTTGCGAAAGTATCCCCGCAGCCTTCCTTCCCCCCGA GCGGACGCAAGAGCGGAGAGCCGGGGAGGGAGAGAGCGGGAGGACGCGTAAAACGCGGTGGAAGACGGCCGCGGGTGCG AGGCTGTCGCTGGGGAAGGACTCCGGCGGCCAAGTACGGGGAGCACGGCAGCCCCGCCATCCTCCTCATGGGCTCCAAT AATGGAGGAGCCGTGCTCAAGCCCGACTCGCAGCAGCCGCTGGTGTGGCCCGCCTGGGTGTACTGCACGCGGTACTCCG ACCGGCCGTCCTCGGGCCCCCGCACCAGGAAGCTGAAGAAGAAGAAGACGGAGAAGGAGGACAAACGGCCGCGCACCGC CTTCACGGCCGAGCAGCTGCAGCGGCTGAAGGCGGAGTTCCAGGCGAACCGCTACATCACGGAGCAGCGGCGGCAGAGC CTGGCCCAGGAGCTGAGCCTCAACGAGTCCCAGATCAAGATCTGGTTCCAGAACAAGCGGGCCAAGATCAAGAAGGCGA CGGGCATCAAGAACGGGCTGGCGCTGCACCTCATGGCCCAGGGACTGTACAACCACTCCACCACCACGGTGCAGGACAA AGAGGAGAGCGAGTGA
Citation	Davey MG, Paton IR, Yin Y, Schmidt M, Bangs FK, Morrice DR, Smith TG, Buxton P, Stamataki D, Tanaka M, Münsterberg AE, Briscoe J, Tickle C, Burt DW. The chicken talpid3 gene encodesa novel protein essentialfor Hedgehog signaling. Genes Dev. 2006 May 15; 20(10): 13651377.
Copyright	Copyright © 2006, Cold Spring Harbor Laboratory Press
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #XM_001234328.2 ).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-20

stage 20

Spinal Cord

Entry 4

GEISHA Id	EN1.Altabef.2002	Data Source	Publication
Complete cDNA Template Probe	show ATGGGTCGTCTCCCACCCCCGGGGCACGGAGCCCCTAAGGCAGCACCCGGGGCACGGAGGCGGCGGGGAGCATCTTCCG ATGACGGCGCGACTCGGGCAAACTTCCCACGCCGGTGGCAGGGAGCCCCGCGCAGCCCCGCGGGTCGCGGTCCCGCGTG CGCCCCTACGGTGGGAGAAGAGCCCGGGGCCGCTCCCAGCCTCGAGGTGCCCCCGCCCGCGGGGCCCCGTCCCGGAGAG CGGCGGGGAGGGCGGAGGCCGGGAGGCGGCAGGCGTCGGCCCTTTGCGAAAGTATCCCCGCAGCCTTCCTTCCCCCCGA GCGGACGCAAGAGCGGAGAGCCGGGGAGGGAGAGAGCGGGAGGACGCGTAAAACGCGGTGGAAGACGGCCGCGGGTGCG AGGCTGTCGCTGGGGAAGGACTCCGGCGGCCAAGTACGGGGAGCACGGCAGCCCCGCCATCCTCCTCATGGGCTCCAAT AATGGAGGAGCCGTGCTCAAGCCCGACTCGCAGCAGCCGCTGGTGTGGCCCGCCTGGGTGTACTGCACGCGGTACTCCG ACCGGCCGTCCTCGGGCCCCCGCACCAGGAAGCTGAAGAAGAAGAAGACGGAGAAGGAGGACAAACGGCCGCGCACCGC CTTCACGGCCGAGCAGCTGCAGCGGCTGAAGGCGGAGTTCCAGGCGAACCGCTACATCACGGAGCAGCGGCGGCAGAGC CTGGCCCAGGAGCTGAGCCTCAACGAGTCCCAGATCAAGATCTGGTTCCAGAACAAGCGGGCCAAGATCAAGAAGGCGA CGGGCATCAAGAACGGGCTGGCGCTGCACCTCATGGCCCAGGGACTGTACAACCACTCCACCACCACGGTGCAGGACAA AGAGGAGAGCGAGTGA
Citation	Altabef M, Tickle C. Initiation of dorso-ventral axis during chick limb development. Mech Dev. 2002 Aug;116(1-2):19-27.
Copyright	Copyright © 2012 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #XM_001234328.2).