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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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FRZB
frizzled related protein

The human orthologue of this gene is associated
with the following human disease:

{Osteoarthritis susceptibility 1}

Gene

Official Symbol	FRZB
Official Full Name	frizzled related protein
CGNC ID	1994
also known as	Frzb-1, secreted frizzled-related protein 3\|frizzled-related protein 1\|sFRP-3, Frizzled- related protein 1 (FrzB-1), FRZB, Secreted frizzled-related protein 3 precursor (sFRP-3)
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204772
Polypeptide	NP_990103
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.4955

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	2487	ENSG00000162998	MIM:165720	MIM:605083
Mouse	20378	ENSMUSG00000027004	All phenotypic alleles (4):Targeted, knock-out(1) Targeted, other(3)	MGI:892032
Xenopus	448021, 734982			481352
Zebrafish	30119	ENSDARG00000018383	ZDB-GENE-990715-1	ZFIN:ZDB-GENE-990715-1

Gene Ontology

Molecular Function	PDZ domain binding, Wnt-activated receptor activity, Wnt-protein binding
Biological Process	axonogenesis, brain development, cochlea morphogenesis, epithelium development, gonad development, more...
Cellular Component	cytoplasm, extracellular space, neuron projection membrane

Links to other databases

Entrez Gene	395545
Ensembl Gene	ENSGALG00000002763
KEGG	gga:395545

Entry 1

GEISHA Id	FRZB.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATAGGCACTGGCATTGAACGC
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGTCCTGACCTTTCCACACCTCAC	cDNA Template Length	741
Forward cDNA Template Probe	show CAATGCAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGG AGGTGAAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCC AAAGGACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATG GGCTACGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTA AAAAAGTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCT GAAGACTGGGAAGCCCNGCAATGCCCGGCAANCTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTAT CTGTCAAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATA ATGTTTTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTGTGTGTGTGTTTGGGGNTGTGCGC ACTCTNNGAATTNTATGTNTTTTTTNTGTGACANAAAAATNTGTAAAACTNNNCTTTTGNGGNGTGGGAAGGNCTCGAG CNNTTTTGTGGGNTTATTTTNNNAGAAAAA

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Epiblast
Neural Plate/Tube

4-6

stage 4

stage 5

stage 6

Neural Plate/Tube
Primitive Streak

Sections pending.

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

stage 12

Blood Vessels
Ear/Otic Placode
Forebrain
Head Mesenchyme
Hindbrain
Lateral Plate Mesoderm
Midbrain
Neural Crest
Neural Plate/Tube

Dorsal view.

13-18

stage 16

Extraembryonic Mesoderm
Head Mesenchyme
Pharyngeal Arches and Clefts
Tail
Trunk Mesenchyme

19-24

stage 19

stage 20

Allantois
Ear/Otic Placode
Eye
Heart
Lateral Plate Mesoderm
Leg
Mesoderm
Oral Pharynx
Pharyngeal Arches and Clefts
Somites
Surface Ectoderm
Widespread Expression
Wing

Sections pending.

25-26

stage 25

stage 26

Ear/Otic Placode
Extraembryonic
Eye
Head Mesenchyme
Lateral Plate Mesoderm
Leg Bones
Leg Mesenchyme
Oral Pharynx
Pharyngeal Arches and Clefts
Somites
Surface Ectoderm
Wing Bones
Wing Mesenchyme

Sections pending.

Entry 2

GEISHA Id	Frzb1.Chapman.2004	Data Source	Publication
Complete cDNA Template Probe	show GGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTCGCCCTGGCCGCGCTGCTGCT GCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGTTATGCAAGCCGCTGCCCTGGAAC ATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGCTGC TGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCAGCA CGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGCCAC GCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCGTCA CCGCCGAGGGCGCCGACTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATGCAA ACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTGAAG ACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGGACA CTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTACGA AGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAAGTA AAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGACTG GGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTCAAG ACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTTTTA GCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCTGGA AATTATATGTTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCTGA AGTAGCCTTTTTAACTCACACGTCTTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTA GTGACCCACATCAGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCACTGCTTGCAAACCTCCTTACTGGGCTG GAGTTTGTTAAGTGCATACATTTTTATTTGAAAACATAACGCCATATACACACACAGGTTACAGTCATATGGCACTTCT GGAAACATAATGAAGAGGAAAAGACCATTTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCAC ATAAATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTA GGAGTAGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCA ATCAGTGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACC TTAACTGACAAAAAAA
Citation	Chapman, S, Brown, R, Lees, L, Schoenwolf, G, Lumsden, A. Expression Analysis of Chick Wnt and Frizzled Genes and Selected Inhibitors in Early Chick Patterning. Dev Dyn. 2004 Mar;229(3):668-76.
Copyright	Wiley-Liss, Inc. 2004
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # AF218057).

Stage

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Location (click to highlight)

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4-5

stage 4

stage 5

Neural Plate/Tube

Entry 3

GEISHA Id	FRZB.Ladher.2000	Data Source	Publication
Complete cDNA Template Probe	show AAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGGACACTGTAAACCTTTACACAAACTCCGGCTGCCT CTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTACGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTG GAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAAGTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCG GCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGACTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAG CTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTCAAGACTTCACTGTTGGAGCAGCAAAGGAGAAATT GCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTTTTAGCTTATATTAGTTTTTATTCTCCCTCTTGTT TTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCTGGAAATTATATGTTTTTTTTATGTTACTGAGAAC TGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCTGAAGTAGCCTTTTTAACTCACACGTCTTCTTAAG GGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAGTGACCCACACCGGCTTCCTCGGCTCAGCCATTCT GATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGGAGTTTAAGTGCATACATTTTTATCTGAAAACATA ACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGAAACATAATGAAGAGGAAAAGACCATTTTTTTCAT CTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAAATTATCATGCAGTTCTTCTCTTATAATGGCAGTT TCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGTAGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGG TCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAGTGCCTATATAAACAAGTGTTCAATACTCAGTAAA ATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAACTGACATTTGAACCAACATCGTATTTTTATATTGC TGTTCAAA
Citation	Ladher RK, Church VL, Allen S, Robson L, Abdelfattah A, Brown NA, Hattersley G, Rosen V, Luyten FP, Dale L, Francis-West PH. Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development. Dev Biol. 2000 Feb 15;218(2):183-98.
Copyright	Reprinted with permission from Elsevier. Copyright © 2000 Elsevier B.V. All rights reserved.
Comments	Information provided in publication was used to obtain the sequence below from NCBI (NM_204772).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 9

stage 12

Forebrain
Lateral Plate Mesoderm
Midbrain
Neural Crest
Neural Plate/Tube

Figure 3 compares the expression of Sfrp-2 and Frzb in the early developing embryo. Figure cover stages HH7, HH9 and HH12.
Rhombomere 6

13-18

stage 13

Hindbrain
Midbrain
Neural Crest

19-24

stage 20

stage 21

stage 23

stage 24

Ear/Otic Placcode
Face Mesenchyme
Heart
Leg Mesenchyme
Neural Crest
Pharyngeal Arches and Clefts
Spinal Ganglia and Nerves
Surface Ectoderm

Central core of the developing limb bud.
Chondrogenic Precursors
Dorsal Root Ganglia
Endocardial Cells
Epibranchial placcodes
Facial Primordia
Figure 7 compares the expression of Sfrp-2 and Frzb in a stage HH24 chick heart.
Lateral Nasal Process
Mandibular Primordium
Maxillary Primordium
Neural Crest-derived Mesenchyme
Outflow and Atrioventricular Cushion Mesenchyme
Sympathetic Ganglia
Ventral Mesenchyme of both Fore- and Hindlimb Buds
Ventral Roots

25-30

stage 27

stage 28

stage 30

Face Mesenchyme
Leg Mesenchyme

Figure 4 compares the expression of Sfrp-2 and Frzb during cranial development.
Figure 5 compares the expression of Sfrp-2 and Frzb in the developing trunk.
Figure 6 compares the expression of Sfrp-2 and Frzb in the developing chick limb at stages HH20/21, HH23, HH27 and HH30.
Frontonasal Mass
Lateral Nasal Process
Meckels Cartilage
Nonchondrogenic Mesenchyme
Prechondrogenic Mesenchyme
maxillary primordia

Entry 4

GEISHA Id	FRZB1.Baranski.2000	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCCGCGCTGCT GCTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGG AACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGC TGCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCA GCACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGC CACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCG TCACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATG CAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTG AAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGG ACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTA CGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAA GTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGA CTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTC AAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTT TTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCT GGAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCT GAAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAG TGACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGG AGTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGA AACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAA ATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGT AGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAG TGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAAC TGACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTA TAAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGA AAGAAATTAAAATGATGTAAATAAAGT
Citation	Baranski M, Berdougo E, Sandler JS, Darnell DK, Burrus LW. The dynamic expression pattern of frzb-1 suggests multiple roles in chick development. Dev Biol. 2000 Jan 1;217(1):25-41.
Copyright	Copyright © 2000 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204772).

Stage

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Location (click to highlight)

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4-6

stage 4

Early Mesoderm
Neural Plate/Tube

Condensed Epithelium rostral and lateral to the Primitive Streak.
FIG. 2. Expression of cfrzb-1, Wnt-3a, Wnt-5a, and Wnt-8c in gastrula-stage chick embryos.

7-12

stage 8

stage 9

stage 11

stage 12

Neural Crest
Neural Plate/Tube

FIG. 3. Comparison of the expression patterns of cfrzb-1, Wnt-1, and Wnt-5a in stage HH8/9 chick embryos.
FIG. 4. Colocalization of cfrzb-1 transcripts and HNK-1 epitopes in the rhombencephalon of a stage HH9 chicken embryo.
FIG. 5. Comparison of the expression patterns of HNK-1, cfrzb-1, and Wnt-1 in stage HH10/11 chicken embryos.
FIG. 6. cfrzb-1 transcripts and HNK-1 epitopes exhibit virtually identical patterns of expression while cfrzb-1 and Wnt-5a are expressed in reciprocal patterns.
Neural Folds

19-24

stage 20

Eye
Heart
Pharyngeal Arches and Clefts
Somites
Spinal Ganglia and Nerves

FIG. 7. Distribution of HNK-1, cfrzb-1, and Wnt-5a in stage HH19/20 chicken embryos.
Nerve Bundles in the anterior half of each Somite

25-30

stage 26

stage 30

Eye
Gonad
Heart
Pharyngeal Arches and Clefts
Sclerotome
Somites
Spinal Ganglia and Nerves
Trunk Mesenchyme

Dorsal Mesentery
Nerve Bundles in the anterior half of each Somite
Surface Epithelium
Vertebrae

31-34

stage 31

stage 34

Leg Bones

Condensing Bone Primordia
FIG. 8. Localization of HNK-1, cfrzb-1, Wnt-5a, Wnt-7a, and sox-9 in whole mounts and sections.

Entry 5

GEISHA Id	Frzb.Chapman.2008	Data Source	Direct Submission
Complete cDNA Template Probe	show GGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTCGCCCTGGCCGCGCTGCTGCT GCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGTTATGCAAGCCGCTGCCCTGGAAC ATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGCTGC TGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCAGCA CGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGCCAC GCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCGTCA CCGCCGAGGGCGCCGACTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATGCAA ACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTGAAG ACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGGACA CTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTACGA AGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAAGTA AAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGACTG GGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTCAAG ACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTTTTA GCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCTGGA AATTATATGTTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCTGA AGTAGCCTTTTTAACTCACACGTCTTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTA GTGACCCACATCAGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCACTGCTTGCAAACCTCCTTACTGGGCTG GAGTTTGTTAAGTGCATACATTTTTATTTGAAAACATAACGCCATATACACACACAGGTTACAGTCATATGGCACTTCT GGAAACATAATGAAGAGGAAAAGACCATTTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCAC ATAAATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTA GGAGTAGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCA ATCAGTGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACC TTAACTGACAAAAAAA
Comments	Images Provided by Susan Chapman

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Epiblast
Neural Plate/Tube

4-6

stage 4

stage 5

stage 6

Neural Plate/Tube

7-11

stage 7

stage 8

stage 9

stage 10

stage 11

Forebrain
Hindbrain
Midbrain
Neural Crest
Neural Plate/Tube
Spinal Cord

Entry 6

GEISHA Id	FRZB.Person.2005	Data Source	Publication
Complete cDNA Template Probe	show GAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCGTCACCGCCGAGGGCGCCGACTTCCCAATGG ATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATGCAAACCCATAAAAGCTACCCAGAAGACCTA TCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTGAAGACTAAATGTCATGATGTCACTGCAGTA GTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGGACACTGTAAACCTTTACACAAACTCCGGCT GCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTACGAAGATGAGGAGCGCTCCAGGTTACTCTT GGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAAGTAAAGCGCTGGGATCAAAAGCTCCGCCAC CTCGGCAAAGGGAAAGGAGAGC
Citation	Person AD, Garriock RJ, Krieg PA, Runyan RB, Klewer SE (2005). Frzb modulates Wnt-9a-mediated beta-catenin signaling during avian atrioventricular cardiac cushion development. Dev Biol. 2005 Feb 1;278(1):35-48.
Copyright	Copyright © 2004 Published by Elsevier Inc.
Comments	The sequence below was obtained from NCBI (AF218057) using the information provided in the publication.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 16

Endocardium
Trunk Mesenchyme

Atrioventricular Canal Cushions

19-24

stage 20

stage 24

Endocardium
Trunk Mesenchyme

Atrioventricular Canal Cushions

Entry 7

GEISHA Id	FRZB.Allen.2001	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCCGCGCTGCT GCTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGG AACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGC TGCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCA GCACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGC CACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCG TCACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATG CAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTG AAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGG ACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTA CGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAA GTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGA CTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTC AAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTT TTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCT GGAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCT GAAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAG TGACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGG AGTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGA AACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAA ATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGT AGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAG TGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAAC TGACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTA TAAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGA AAGAAATTAAAATGATGTAAATAAAGT
Citation	Allen SP, Bogardi JP, Barlow AJ, Mir SA, Qayyum SR, Verbeek FJ, Anderson RH, Francis-West PH, Brown NA, Richardson MK (2001). Misexpression of noggin leads to septal defects in the outflow tract of the chick heart. Dev Biol. 2001 Jul 1;235(1):98-109.
Copyright	Copyright © 2001 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204772).

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 25

Heart
Outflow Tract

Atrioventricular Canal

Entry 8

GEISHA Id	FRZB1.Jin.2001	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCCGCGCTGCT GCTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGG AACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGC TGCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCA GCACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGC CACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCG TCACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATG CAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTG AAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGG ACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTA CGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAA GTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGA CTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTC AAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTT TTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCT GGAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCT GAAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAG TGACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGG AGTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGA AACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAA ATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGT AGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAG TGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAAC TGACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTA TAAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGA AAGAAATTAAAATGATGTAAATAAAGT
Citation	Jin EJ, Erickson CA, Takada S, Burrus LW (2001). Wnt and BMP signaling govern lineage segregation of melanocytes in the avian embryo. Dev Biol. 2001 May 1;233(1):22-37.
Copyright	Copyright © 2001 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204772).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 13

Neural Crest

Entry 9

GEISHA Id	Frzb1.Theodosiou.2003	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCCGCGCTGCT GCTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGG AACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGC TGCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCA GCACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGC CACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCG TCACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATG CAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTG AAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGG ACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTA CGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAA GTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGA CTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTC AAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTT TTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCT GGAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCT GAAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAG TGACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGG AGTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGA AACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAA ATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGT AGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAG TGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAAC TGACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTA TAAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGA AAGAAATTAAAATGATGTAAATAAAGT
Citation	Theodosiou NA, Tabin CJ. Wnt signaling during development of the gastrointestinal tract. Dev Biol. 2003 Jul 15;259(2):258-71.
Copyright	Copyright © 2003 Elsevier Science (USA). All rights reserved.
Comments	Authors indicated probe was made from a ~640 bp fragment. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204772).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 22

stage 24

Duodenum
Foregut
Gizzard
Gut
Hindgut

31-32

stage 32

Duodenum
Hindgut

Only partial expression in the large intestines.

Entry 10

GEISHA Id	Frzb1.McBride.2003	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCCGCGCTGCT GCTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGG AACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGC TGCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCA GCACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGC CACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCG TCACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATG CAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTG AAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGG ACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTA CGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAA GTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGA CTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTC AAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTT TTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCT GGAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCT GAAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAG TGACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGG AGTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGA AACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAA ATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGT AGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAG TGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAAC TGACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTA TAAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGA AAGAAATTAAAATGATGTAAATAAAGT
Citation	McBride HJ, Fatke B, Fraser SE. Wnt signaling components in the chicken intestinal tract. Dev Biol. 2003 Apr 1;256(1):18-33.
Copyright	With permission from Elsevier Copyright © 2003 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204772)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-30

stage 26

stage 29

stage 30

Hindgut

Entry 11

GEISHA Id	SFRP3.Quinlan.2009	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCGCGCTGCTG CTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGGA ACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGCT GCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCAG CACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGCC ACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCGT CACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATGC AAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTGA AGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGGA CACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTAC GAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAAG TAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGAC TGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTCA AGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTTT TAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCTG GAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCTG AAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAGT GACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGGA GTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGAA ACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAAA TTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGTA GACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAGT GCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAACT GACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTAT AAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGAA AGAAATTAAAATGATGTAAATAAAGT
Citation	Quinlan R, Graf M, Mason I, Lumsden A, Kiecker C. Complex and dynamic patterns of Wnt pathway gene expression in the developing chick forebrain. Neural Dev. 2009 Sep 4;4:35.
Copyright	Copyright © 2009 Quinlan et al; licensee BioMed Central Ltd. [This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.]
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204772)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 13

stage 15

Diencephalon
Midbrain
Neural Plate/Tube

19-20

stage 20

Diencephalon
Midbrain
Neural Plate/Tube

Entry 12

GEISHA Id	FRZB1.Geetha-Loganathan.2009	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCCGCGCTGCT GCTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGG AACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGC TGCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCA GCACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGC CACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCG TCACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATG CAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTG AAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGG ACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTA CGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAA GTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGA CTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTC AAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTT TTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCT GGAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCT GAAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAG TGACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGG AGTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGA AACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAA ATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGT AGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAG TGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAAC TGACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTA TAAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGA AAGAAATTAAAATGATGTAAATAAAGT
Citation	Geetha-Loganathan, P, Nimmagadda, S, Antoni, L, Fu, K, Whiting, C, Francis-West, P and Richman, J. Expression of WNT signalling pathway genes during chicken craniofacial development. DEVELOPMENTAL DYNAMICS Volume 238, Issue 5, May 2009, Pages: 11501165.
Copyright	Dev. Dyn Copyright © 2009 John Wiley & Sons, Inc. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204772 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 17

Face Mesenchyme
Head Mesenchyme
Nasal Placode/Nerve
Pharyngeal Arches and Clefts

19-24

stage 21

stage 24

Face Mesenchyme
Head Mesenchyme
Pharyngeal Arches and Clefts

25-28

stage 25

stage 28

Face Mesenchyme
Head Mesenchyme
Pharyngeal Arches and Clefts

Entry 13

GEISHA Id	FRZB.Sienknecht.2009	Data Source	Publication
Complete cDNA Template Probe	show CTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGGACACTGTAAACCTTTACACAAA CTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTACGAAGATGAGGAGCGCTCCAGG TTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAAGTAAAGCGCTGGGATCAAAAGC TCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGACTGGGAAGCCCGGCAATGCCCG GCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTCAAGACTTCACTGTTGGAGCAGC AAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTTTTAGCTTATATTAGTTTTTATT CTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCTGGAAATTATATGTTTTTTTTAT GTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCTGAAGTAGCCTTTTTAACTCACA CGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAGTGACCCACACCGGCTTCCTCGG CTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGGAGTTTAAGTGCATACATTTTTA TCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGAAACATAATGAAGAGGAAAAGAC CATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAAATTATCATGCAGTTCTTCTCTT ATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGTAGACTGCCTTACCAGGCCTGCT TCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAGTGCCTATATAAACAAGTGTTCA ATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAACTGACA
Citation	Sienknecht UJ, Fekete DM. Mapping of Wnt, frizzled, and Wnt inhibitor gene expression domains in the avian otic primordium. J Comp Neurol. 2009 Dec 20;517(6):751-64.
Copyright	Copyright © (2002 Wiley-Liss); This material is used by permission of Wiley-Liss, Inc., a subsidiary of John Wiley & Sons, Inc.
Comments	NCBI Acc.# provided in paper.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

13-18

stage 16

stage 17

stage 18

Ear/Otic Placode
Rhombomeres

19-24

stage 20

stage 22

stage 24

Ear/Otic Placode

Entry 14

GEISHA Id	FRZB.Tzahor.2003	Data Source	Publication
Complete cDNA Template Probe	show CGCGGCGCTCGGCCCCTGAGCTCGGGGCCGTCGGCGGCATGTGGCGGGGGCTGCCGGCGCTGGCCCTGGCCGCGCTGCT GCTGCTGGGGCGGGCGCCGGCGGGGCGGGCGGCGGCCTGCGAGCCGGTGCGCATCCCGCTATGCAAGCCGCTGCCCTGG AACATGACCAAGATGCCGAACCACCTGCATCACAGCACGCAGGCCAACGCCGTGCTGGCCATGGAGCAGTTCGAGGGGC TGCTGGGCACCAACTGCAGCCCGGACCTGCTCTTCTTCCTCTGCGCCATGTACGCGCCCATCTGCACCATCGACTTCCA GCACGAGCCCATCAAGCCCTGCAAGTCCGTCTGCGAGCGCGCCCGCGCCGGCTGCGAGCCCGTCCTCATCCGCTACCGC CACGCGTGGCCCGAGAGCCTGGCCTGCGACGAGCTGCCGCTCTACGACCGCGGCGTCTGCATCTCCCCCGAGGCCATCG TCACCGCCGAGGGCGCCGATTTCCCAATGGATTCTAATAATGGCAACTGTAGAGGCACTGGCATTGAACGCTGCAAATG CAAACCCATAAAAGCTACCCAGAAGACCTATCTACGCAACAATTACAACTATGTCATTCGAGCAAAAGTGAAGGAGGTG AAGACTAAATGTCATGATGTCACTGCAGTAGTGGAAGTAAAAGAGATTCTCAAATCTTCCCTGGTGAACATCCCAAAGG ACACTGTAAACCTTTACACAAACTCCGGCTGCCTCTGTCCACCACTCAGTGCCAATGAAGAGTACATCATTATGGGCTA CGAAGATGAGGAGCGCTCCAGGTTACTCTTGGTGGAAGGCTCCATAGCTGAGAAATGGAAGGATCGACTTGGTAAAAAA GTAAAGCGCTGGGATCAAAAGCTCCGCCACCTCGGCAAAGGGAAAGGAGAGCCGGGACAAAGTGATTCGGCGCTGAAGA CTGGGAAGCCCGGCAATGCCCGGCAAACTCGCAGCTAGATGTGAAATGCCCTACGCCAAAATTCAGTGGACTATCTGTC AAGACTTCACTGTTGGAGCAGCAAAGGAGAAATTGCACTATTGCACGTTATATTCTATTGTTTACTACAGAATAATGTT TTAGCTTATATTAGTTTTTATTCTCCCTCTTGTTTTCTACCTTTTTTTGTTTGTTTGTTTTTGGATGTGTGCAAACTCT GGAAATTATATGTTTTTTTTATGTTACTGAGAACTGTAGAAAACTGCCCTTGTGAGGTGTGGAAAGGTCAGGATATGCT GAAGTAGCCTTTTTAACTCACACGTCTTCTTAAGGGGAGAACCTATGCAAGCAGGAGATCTAGTGCAGCCCATCAGTAG TGACCCACACCGGCTTCCTCGGCTCAGCCATTCTGATCACAGCCTTGGTCGCTGCTTGCAAACCTCCTTACTGGGCTGG AGTTTAAGTGCATACATTTTTATCTGAAAACATAACGGCCATATACACACACAGGTTACAGTCATATGGCACTTCTGGA AACATAATGAAGAGGAAAAGACCATTTTTTTCATCTCTGGACCTTGTAGGAAACCTTATGTAGTTCTGCCACCACATAA ATTATCATGCAGTTCTTCTCTTATAATGGCAGTTTCTTCTTCTTTTTTTTTTTCCTTCTTTTTGCTGAGTTGTAGGAGT AGACTGCCTTACCAGGCCTGCTTCTCAGTCCAGGTCTGCACTTTATCATTTTGACTTTGTGGTGTTATTCTTCAATCAG TGCCTATATAAACAAGTGTTCAATACTCAGTAAAATATGTCCAATAAAAAAAATTGTAGCTGTCAATATGGACCTTAAC TGACATTTGAACCAACATCGTATTTTTATATTGCTGTTCAAAATATAAAACCCCAAATCCCTTCCTTTTTGTGCTTTTA TAAAACTCTTTGCAGATAGTAATAACACGTTTGAAATTAATCACTCTGTTCTTCTGTTTTTCTTTTCTCTCTTGCTTGA AAGAAATTAAAATGATGTAAATAAAGT
Citation	Tzahor E, Kempf H, Mootoosamy RC, Poon AC, Abzhanov A, Tabin CJ, Dietrich S, Lassar AB. Antagonists of Wnt and BMP signaling promote the formation of vertebrate head muscle. Genes Dev. 2003 Dec 15;17(24):3087-99.
Comments	As insufficient information is provided in publication to verify the exact sequence used to synthesize probe, the sequence was obtained from NCBI (acc #NM_204772.2).