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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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SFRP2
secreted frizzled related protein 2

Gene

Official Symbol	SFRP2
Official Full Name	secreted frizzled related protein 2
CGNC ID	7021
also known as	SFRP-2, secreted frizzled-related protein 2, sFRP-2, Sdf5, Secreted frizzled-related protein 2 precursor
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204773
Polypeptide	NP_990104
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.4345

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	6423	ENSG00000145423		MIM:604157
Mouse	20319	ENSMUSG00000027996	All phenotypic alleles (7):Chemically induced(2) Targeted, knock-out(4) Targeted, other(1)	MGI:108078
Xenopus	394659, 380355			485146
Zebrafish	566878		ZDB-GENE-061013-293	ZFIN:ZDB-GENE-061013-293

Gene Ontology

Molecular Function	PDZ domain binding, Wnt-activated receptor activity, Wnt-protein binding
Biological Process	apoptotic process, brain development, canonical Wnt signaling pathway, cell differentiation, more...
Cellular Component	cell projection, cytoplasm, extracellular region, plasma membrane

Links to other databases

Entrez Gene	395546
Ensembl Gene	ENSGALG00000009241
KEGG	gga:395546

Entry 1

GEISHA Id	sFRP2.Chapman.2004	Data Source	Publication
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTAACCTAACCTGAAAAAGTTGTAAATATTGCTTTAATTAACCTTGTAAATACTTCAGATAAATG TTATATTCTTGTATATAAACTTTACATGGTAGTTTTAAAAAAAACCATGG
Citation	Chapman, S, Brown, R, Lees, L, Schoenwolf, G, Lumsden, A. Expression Analysis of Chick Wnt and Frizzled Genes and Selected Inhibitors in Early Chick Patterning. Dev Dyn. 2004 Mar;229(3):668-76.
Copyright	Wiley-Liss, Inc. 2004

Stage

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Location (click to highlight)

Comments (click to highlight)

4-5

stage 4

stage 5

Epiblast
Neural Plate/Tube

Entry 2

GEISHA Id	SFRP2.Ladher.2000	Data Source	Publication
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTAACCTAACCTGAAAAAGTTGTAAATATTGCTTTAATTAACCTTGTAAATACTTCAGATAAATG TTATATTCTTGTATATAAACTTTACATGGTAGTTTTAAAAAAAACCATGG
Citation	Ladher RK, Church VL, Allen S, Robson L, Abdelfattah A, Brown NA, Hattersley G, Rosen V, Luyten FP, Dale L, Francis-West PH. Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development. Dev Biol. 2000 Feb 15;218(2):183-98.
Copyright	Reprinted with permission from Elsevier. Copyright © 2000 Elsevier B.V. All rights reserved.
Comments	According to the authors, "The Sfrp-2 antisense probe was generated by linearising the full-length clone with HindIII and transcribing with T3 RNA polymerase." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_204773).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 9

stage 12

Dermomyotome
Ear/Otic Placcode
Forebrain
Hindbrain
Neural Plate/Tube
Rhombomeres
Spinal Cord

Dorsal Prosencephalon
Figure 3 compares the expression of Sfrp-2 and Frzb in the early developing embryo. Figure cover stages HH7, HH9 and HH12.
Rhombomeres 2, 4, and 6

13-18

stage 13

Ear/Otic Placcode
Forebrain
Hindbrain
Nasal Placcode/Nerve
Neural Plate/Tube

19-24

stage 20

stage 21

stage 23

stage 24

Ear/Otic Placcode
Ectoderm
Face Mesenchyme
Leg Mesenchyme
Nasal Placcode/Nerve
Pharyngeal Arches and Clefts
Surface Ectoderm
Ventricles

Figure 7 compares the expression of Sfrp-2 and Frzb in a stage HH24 chick heart.
Mandibular Primordia
Posterior Maxillary Primordia
Proximal Mesenchyme
Trabeculae
ectoderm of the facial primordia

25-30

stage 27

stage 28

stage 30

Dermomyotome
Face Mesenchyme
Leg Mesenchyme
Myotome

Figure 4 compares the expression of Sfrp-2 and Frzb during cranial development.
Figure 5 compares the expression of Sfrp-2 and Frzb in the developing trunk.
Figure 6 compares the expression of Sfrp-2 and Frzb in the developing chick limb at stages HH20/21, HH23, HH27 and HH30.
Intermandibularis Muscle
Migrating Muscle Precursors
Muscle Medial to the Eye
Proximal Mesenchyme

Entry 3

GEISHA Id	Sdf5.Chapman.2008	Data Source	Direct Submission
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTAACCTAACCTGAAAAAGTTGTAAATATTGCTTTAATTAACCTTGTAAATACTTCAGATAAATG TTATATTCTTGTATATAAACTTTACATGGTAGTTTTAAAAAAAACCATGG
Comments	Images Provided by Susan Chapman.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-11

stage 10

stage 11

Forebrain
Hindbrain
Midbrain
Neural Plate/Tube
Spinal Cord

Entry 4

GEISHA Id	sFRP2.Chapman.2008	Data Source	Direct Submission
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTAACCTAACCTGAAAAAGTTGTAAATATTGCTTTAATTAACCTTGTAAATACTTCAGATAAATG TTATATTCTTGTATATAAACTTTACATGGTAGTTTTAAAAAAAACCATGG
Comments	Images Provided by Susan Chapman.

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Unlabeled

4-6

stage 4

stage 5

stage 6

Epiblast
Neural Plate/Tube
Primitive Streak

7-11

stage 7

stage 8

stage 9

stage 10

stage 11

Anterior Intestinal Portal
Forebrain
Hindbrain
Neural Plate/Tube
Rhombomeres
Spinal Cord
Surface Ectoderm

Entry 5

GEISHA Id	sFRP2.Theodosiou.2003	Data Source	Publication
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTAACCTAACCTGAAAAAGTTGTAAATATTGCTTTAATTAACCTTGTAAATACTTCAGATAAATG TTATATTCTTGTATATAAACTTTACATGGTAGTTTTAAAAAAAACCATGG
Citation	Theodosiou NA, Tabin CJ. Wnt signaling during development of the gastrointestinal tract. Dev Biol. 2003 Jul 15;259(2):258-71.
Copyright	Copyright © 2003 Elsevier Science (USA). All rights reserved.
Comments	Authors indicated probe was 224 bp long. Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_204773.1)

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 22

stage 24

Duodenum
Foregut
Hindgut
Midgut

Ceca

31-32

stage 32

Duodenum
Hindgut
Midgut

Entry 6

GEISHA Id	SFrp2.McBride.2003	Data Source	Publication
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTAACCTAACCTGAAAAAGTTGTAAATATTGCTTTAATTAACCTTGTAAATACTTCAGATAAATG TTATATTCTTGTATATAAACTTTACATGGTAGTTTTAAAAAAAACCATGG
Citation	McBride HJ, Fatke B, Fraser SE. Wnt signaling components in the chicken intestinal tract. Dev Biol. 2003 Apr 1;256(1):18-33.
Copyright	With permission from Elsevier Copyright © 2003 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204773).

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

Gut

19-24

stage 23

Hindgut
Midgut

25-30

stage 26

stage 28

Gut
Midgut

31-34

stage 34

Midgut

Entry 7

GEISHA Id	SFRP2.Quinlan.2009	Data Source	Publication
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTAACCTAACCTGAAAAAGTTGTAAATATTGCTTTAATTAACCTTGTAAATACTTCAGATAAATG TTATATTCTTGTATATAAACTTTACATGGTAGTTTTAAAAAAAACCATGG
Citation	Quinlan R, Graf M, Mason I, Lumsden A, Kiecker C. Complex and dynamic patterns of Wnt pathway gene expression in the developing chick forebrain. Neural Dev. 2009 Sep 4;4:35.
Copyright	Copyright © 2009 Quinlan et al; licensee BioMed Central Ltd. [This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.]
Comments	Authors indicated probe was 224 nt long. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204773).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 13

stage 16

Forebrain
Hindbrain
Midbrain

19-20

stage 20

Forebrain
Hindbrain
Midbrain

Entry 8

GEISHA Id	SFRP2.Sienknecht.2009	Data Source	Publication
Complete cDNA Template Probe	show CTCCGCTCAGCCCACCGCCCCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGA CCGCCGGGCTCTTCCCTTTCGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCT GCTGTGCCGAGGCATCGAGTACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAG CAGGCCACCACCTGGATCCCGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCC CTGTCTGCATCGACGACCTGGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACC AGTGATGTCTGCTTTCGGCTTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATC CCGCTGGCCAGCAGTGACCACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATG AGGATGATAACGACATCATGGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACAT CAATGGGGATACCAAGATCACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTG AGGAAGATTGTGCTCTGGCTCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGG TGATGGGGCAAAAGCAAGCTGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCG GTTCTCCCGCAGCATCCGCAAACTGCAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGC TCTCGAGGCCTCCCCAACCTTCTTTCTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATC TCCGAAGAGGGGGGAGCCCTTTGTTTCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGA AGTATTAAACTATCTCACTTAACAGGTTTTTTTTTCTTGTTTGTTTTTTTAATGGTTGCAAATGTGACTCTTATCTGAG GTTTTTCCCTCTTCATTTTGGTAATGCTGGTTACATTTATATTGCTGCCTCTCTGTATACATGCATGTTTGTTGTGCAA AAGTAGGAAACAAAGAAAGACAGACGTGTGCCACTGGCTTGTCACAAGGGGTACGACTATTTCACTGAGGTGAAATTGC TTGCCATCTATATTAAATTTATAAAGGTCATGTTACAACTCTTGCATGTGATACATAGGCACCAGTAAGGGTATATTAA CTATTCTCAAGTTTTATTTTCCACTGTATTTTCCACCATAAAGACTGTGCCCTTTTCTTAAGAAAAAAAACAAAACAAA ACAGGTTTTGGAACTGGTTTATATGAATGCAACTTTTTTCTTATTATTTTGCAAATTAAACCATCTGTAGCCTAACTGT AACATACCTAGTGGCTC
Citation	Sienknecht UJ, Fekete DM. Mapping of Wnt, frizzled, and Wnt inhibitor gene expression domains in the avian otic primordium. J Comp Neurol. 2009 Dec 20;517(6):751-64.
Copyright	Copyright © (2002 Wiley-Liss); This material is used by permission of Wiley-Liss, Inc., a subsidiary of John Wiley & Sons, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204773)

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 16

Ear/Otic Placode

19-24

stage 20

stage 24

Ear/Otic Placode

double labeled

Entry 9

GEISHA Id	SFRP2.LinCT.2007	Data Source	Publication
Complete cDNA Template Probe	show CCGCCATGCCGCGCCGCCTCTGCGCCCTGCTCCTGCTGGCCTCCCAGTGCCTGGGTTCGACCGCCGGGCTCTTCCCTTT CGGGGAGCCCGACTTCTCCTACAAGCGCTCCAACTGCAAGCCCATCCCAGCCCCGATGCTGCTGTGCCGAGGCATCGAG TACCAGAGCATGCGGCTGCCCAACCTGCTGGGGCATGAGACGGTGCAGGAGGTGCTGGAGCAGGCCACCACCTGGATCC CGCTGGTGCAGAAGCAGTGTCACCCCGATACTAGGAAGTTCCTCTGCTCCCTCTTTGCCCCTGTCTGCATCGACGACCT GGACGAGATCATCCAGCCCTGCCACTCACTCTGCGAGGAGGTAAAGGAGAGCTGTGCACCAGTGATGTCTGCTTTCGGC TTCCCCTGGCCCGACATGCTGGACTGCAGCCGCTTCCCCAAGGACAATGACCTTTGCATCCCGCTGGCCAGCAGTGACC ACATCCTCCCAGTCACCAGGGAAGCACCCAAAGTCTGCGATGCCTGCAAGAACAAAAATGAGGATGATAACGACATCAT GGAAAACCTCTGCAAAAATGACTTTGCCTTGAAGATAAAAGTGAAGGAGATTGCCTACATCAATGGGGATACCAAGATC ACCCCTGAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTGAGGAAGATTGTGCTCTGGC TCAAAGGTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGGTGATGGGGCAAAAGCAAGC TGGGGAACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCGGTTCTCCCGCAGCATCCGC AAACTGCAGTGTTAGTGGCTTTGG
Citation	Lin CT, Lin YT, Kuo TF. Investigation of mRNA expression for secreted frizzled-related protein 2 (sFRP2) in chick embryos. J Reprod Dev. 2007 Aug;53(4):801-10. Epub 2007 May 10.
Copyright	© 2012 researchgate.net. All rights reserved.
Comments	Authors indicate the probe was amplified by PCR using the following primers: 5_-ccgccatggcgcgccgcctc-3_ and 5_-ccaaagcttctaacactgcag-3_ and was extracted from NCBI Acc# NM_204773.1. Primer and sequence match is imperfect.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

Endoderm
Neural Plate/Tube
Notochord
Somites
Spinal Cord

13-18

stage 18

Central Nervous System
Myotome
Neural Plate/Tube
Notochord
Pharynx
Retina
Sclerotome

19-24

stage 23

Eye
Mesencephalon
Myotome
Neural Plate/Tube
Sclerotome

25-30

stage 26

stage 28

stage 30

Central Nervous System
Eye
Face Mesenchyme
Metencephalon
Myelencephalon
Retina
Rhombomeres
Sclerotome
Somites
Spinal Cord
Spinal Ganglia and Nerves
Vertebrae

glia

31-36

stage 31

stage 34

stage 35

stage 36

Diencephalon
Face Mesenchyme
Leg Bones
Retina
Spinal Cord
Spinal Ganglia and Nerves
Telencephalon
Vertebrae
Wing
Wing Bones

neurons

Entry 10

GEISHA Id	SFRP2.Singh.2016	Data Source	Publication
Complete cDNA Template Probe	show CACGGTTTCAGGAATACATCCTGTCCCAGCAAAAACTGGAGTGCTGCTTAACATAGCAAAGCTGTCCTCTTTCTATTGC AAACTGGAGAAAATATTAATCCTTGAAGATAAAAGTGAAGGAGATTGCCTACATCAATGGGGATACCAAGATCACCCCT GAAACAAAGAGCAAGACCATCTACAAGCTGAATGGGCTGACTGAAAGGGATCTGAGGAAGATTGTGCTCTGGCTCAAAG GTGGCCTCCAGTGTACCTGTGACGAGATGAATGACATCAATGTCCCCTACTTGGTGATGGGGCAAAAGCAAGCTGGGGA ACTGGTGATCACCTCACTGAAGCGGTGGCAGAAAGGGCAGCGAGCTTTTAAGCGGTTCTCCCGCAGCATCCGCAAACTG CAGTGTTAGTGGCTTTGGCTGCGTTGGCTTCCAGCAGCCTCCTCTGACCCAAGCTCTCGAGGCCTCCCCAACCTTCTTT CTTTAGCCTCTCTACACCTCGAGATGCCCTGGCCAGGAGACACAGCAACCAATCTCCAAGAGGGGGAGAGCCCTTTGTT TCTGTACATAGGTTAAATGTAATAAAAATATATATATCATGACTATTTGGGGGAAGTATTAAACTATCTCACTTAACAG GTTTTTTTTCTTGTGGTTTTAATGGTTGCAAATGTGACTCTTATCTGAGGTTTTTCCCTCTTCATTTGGTATGCTG
Citation	Singh PN, Ray A, Azad K, Bandyopadhyay A. A comprehensive mRNA expression analysis of developing chicken articular cartilage. Gene Expr Patterns. 2016 Jan;20(1):22-31.
Copyright	Copyright © 2015 Elsevier B.V. All rights reserved.
Comments	The complete cDNA template sequence was obtained from the information provided in the publication as described in Singh et al. 2016. The probe was synthesized from cDNA clone ChEST712d9.