Authors said: A linearized plasmid of chick Id2 (either full length or from nucleotide 554 to 848) was used as a template to generate a digoxigenin-labeled antisense probe (Martinsen and Bronner-Fraser, 1998). Probe sequence used was from Acc# NM_205002.1)
Authors indicated the probe was made from gene construct provided by M. Bronner. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence above was obtained from NCBI (accession number NM_205002.1).
Images provided by Claire Anderson. As insufficient information was provided to verify sequence used to synthesize probe, the sequence above was obtained from NCBI (accession number NM_205002.2). Reference paper: Anderson C, Khan MAF, Wong F, Solovieva T, Oliveira NMM, Baldock RA, Tickle C, Burt DW, Stern CD. A strategy to discover new organizers identifies a putative heart organizer. Nat Commun. 2016 7:12656. doi: 10.1038/ncomms12656.