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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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SEMA3A
semaphorin 3A

Gene

Official Symbol	SEMA3A
Official Full Name	semaphorin 3A
CGNC ID	4946
also known as	COLL-1, semaphorin-3A, semaphorin-3A\|collapsin\|collapsin-1\|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204977
Polypeptide	NP_990308
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.487

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly	36846			FBgn0011260
Human	10371	ENSG00000075213		MIM:603961
Mouse	20346	ENSMUSG00000028883	All phenotypic alleles (8):Chemically induced(1) Targeted, knock-out(2) Targeted, other(5)	MGI:107558
Xenopus	100494110, 378645			966368
Zebrafish	30266, 30267	ENSDARG00000019235, ENSDARG00000042210	ZDB-GENE-991209-3, ZDB-GENE-991209-6	ZFIN:ZDB-GENE-991209-3, ZFIN:ZDB-GENE-991209-6

Gene Ontology

Molecular Function	receptor activity
Biological Process	cell differentiation, nervous system development
Cellular Component	extracellular region, membrane

Links to other databases

Entrez Gene	395825
Ensembl Gene	ENSGALG00000006560
KEGG	gga:395825

Entry 1

GEISHA Id	Sema3A.Lwigale.2007	Data Source	Publication
Complete cDNA Template Probe	show CGTGAAAATGCGATTGATGGAGAGCACACTGGAAAAGCCACTCATGCCAGAATAGGGCAGATCTGTAAGAATGACTTTG GTGGACACAGGAGCCTTGTTAACAAATGGACAACTTTCCTCAAAGCACGTCTGATTTGTTCTGTGCCAGGACCCAATGG TATTGACACACACTTTGATGAATTACAGGACGTGTTCCTAATGAACTCTAAGGACCCTAAAAATCCAATAGTGTATGGA GTGTTTACAACTTCCAGTAATATCTTCAAGGGATCAGCAGTATGTATGTACAGCATGACTGATGTGAGGAGGGTATTTC TTGGCCCCTATGCCCACCGAGATGGCCCAAACTACCAGTGGGTTCCCTACCAAGGGCGAGTTCCATATCCACGGCCAGG AACTTGCCCCAGTAAAACATTTGGCGGCTTTGACTCCACCAAGGACCTTCCTGATGAAGTTATAACATTTGCAAGAAGT CATCCTGCCATGTATAACCCAGTATTCCCCATCAACAGCCGTCCAAT
Citation	Lwigale PY, Bronner-Fraser M. Lens-derived Semaphorin3A regulates sensory innervation of the cornea. Dev Biol. 2007 Jun 15;306(2):750-9. Epub 2007 Apr 18.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Authors said: Sema3A; forward 5′-cgtgaaaatgcgattgatggagag-3′, reverse 5′-attggacggctgttgatggg-3′) used as template for sense and antisense probe synthesis. From this, probe was determined from NM_204977.1.

Stage

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Location (click to highlight)

Comments (click to highlight)

25-30

stage 26

Eye
Lens
Retina

31-36

stage 34

stage 36

Eye
Lens
Retina

Entry 2

GEISHA Id	Sema3A.Masuda.2003	Data Source	Publication
Complete cDNA Template Probe	show GCTGATGGAAGACTTTGTTACTTGTTTCTAGCTGGGAGCGACCCAAAGAGGATTATGTTATGGGCAGAAGATGTTATTT ATGTTGCCTGAAGTTCCCCGGCGGCGCCCGGCAGGACGGGGAGCGCCGCGGCTCCGGCTCGCAGGGACCCTACAGTGGC TGCTGCAGCATGGGCTGGCTCAGGGGGATTGCCTTGCTTTCCTTGGGAGTATTACTAGCAGGAAGAGTGAACTGCCAGC ATGTGAAGAATAACGTGCCAAGACTCAAATTATCCTATAAAGAAATGTTGGAATCCAATAATATAGTCAATTTCAATGG ACTAGCTAATAGTTCCAGTTACCATACTTTCCTCTTGGATGAAGAACGAAGTCGGCTGTATGTTGGAGCAAAAGACCAC ATATTTTCTTTCAACCTGGTTAACATCAAGGAATATCAAAAGATTGTTTGGCCTGTATCTCATTCCCGAAGGGATGAGT GTAAGTGGGCTGGAAAAGATATTCTGAGAGAATGTGCTAATTTCATCAAGGTGCTTAAGACTTATAACCAAACACACTT GTACGCTTGTGGAACGGGGGCTTTTCATCCCATGTGCACTTATATTGAAGTTGGAAGCCATCCTGAGGACAACATTTTT AGGATGGAAGATTCACATTTTGAAAACGGCCGAGGGAAAAGCCCTTACGATCCTAAATTGCTGACAGCTTCTCTTTTAG TAGATGGAGAATTGTACTCTGGCACAGCAGCAGACTTCATGGGCAGAGACTTTGCCATTTTCCGAACTCTGGGGCATCA CCATCCAATCAGAACAGAGCAGCATGACTCCCGGTGGCTAAACGATCCCAGATTTATTAGTGCTCACCTGATACCTGAA AGTGATAATCCAGAAGATGACAAAATCTATTTCTTCTTTCGTGAAAATGCGATTGATGGAGAGCACACTGGAAAAGCCA CTCATGCCAGAATAGGGCAGATCTGTAAGAATGACTTTGGTGGACACAGGAGCCTTGTTAACAAATGGACAACTTTCCT CAAAGCACGTCTGATTTGTTCTGTGCCAGGACCCAATGGTATTGACACACACTTTGATGAATTACAGGACGTGTTCCTA ATGAACTCTAAGGACCCTAAAAATCCAATAGTGTATGGAGTGTTTACAACTTCCAGTAATATCTTCAAGGGATCAGCAG TATGTATGTACAGCATGACTGATGTGAGGAGGGTATTTCTTGGCCCCTATGCCCACCGAGATGGCCCAAACTACCAGTG GGTTCCCTACCAAGGGCGAGTTCCATATCCACGGCCAGGAACTTGCCCCAGTAAAACATTTGGCGGCTTTGACTCCACC AAGGACCTTCCTGATGAAGTTATAACATTTGCAAGAAGTCATCCTGCCATGTATAACCCAGTATTCCCCATCAACAGCC GTCCAATCATGATCAAAACGGATGTAGATTACCAGTTCACACAGATAGTAGTAGATCGAGTAGATGCAGAAGATGGTCA ATACGATGTTATGTTCATTGGCACAGATATTGGAACAGTTCTTAAAGTGGTTTCCATTCCTAAGGAGACTTGGCATGAA TTAGAAGAAGTCTTGCTGGAAGAAATGACCGTCTTTCGGGAACCCACTGTTATTTCAGCAATGAAGATTTCCACAAAAC AGCAACAGCTCTACATTGGCTCAGCCACCGGAGTTTCACAGCTTCCTTTACACCGCTGTGATGTCTATGGAAAAGCATG TGCTGAGTGTTGTCTTGCAAGAGACCCCTACTGCGCCTGGGATGGTTCATCTTGCTCACGTTACTTTCCCACTGCTAAG AGACGTACTAGGCGACAAGACATCCGAAATGGAGATCCCCTCACTCACTGCTCAGACCTGCAGCATCATGATAACCCAA GTGGTCAGACCCTGGAGGAAAAGATTATTTATGGAGTAGAGAATAGCAGCACTTTTCTTGAGTGCAGTCCAAAATCTCA ACGTGCCATAGTCTACTGGCAATTCCAGAAGCAAAATGATGACCACAAAGTTGAGATAAAAGTGGATGATCGAATGATC CGGACAGAACAGGGCCTATTGCTACGAAGTCTTCAACGGAGAGACTCTGGTATTTATTTTTGTCATGCTGTGGAGCATG GCTTCATTCAAACTTTGCTCAAAGTGACACTGGAAGTAATTGATACTGACCACTTGGAAGAGCTGCTCCATAAGGAAGA AGACGCCGATGCCTCCAAGACCAAGGATGCTACCAATAGCATGACTCCTAGTCAAAAGATCTGGTATAGAGACTTCATG CAGTTAATCAATCACCCCAATCTCAACACAATGGATGAATTCTGTGAGCAGGTTTGGAAAAGAGACCGCAAGCAGCGCC GGCAAAGGCCTGCCAATGCGCAGGTGAACACAAATAAGTGGAAGCACCTACAAGAGAATAAAAAAGGTAGGAATAGGAG GACCCATGAATTTGAGAGGGCACCACGGAGTGTCTGAGCTACATTACCTCTAGAAACCTCATCTAAGTAGAAACTTGTA TAGACAGATAACTGGAAAAAAAATGCAATATACATGAACTTTTTCATGGCATTAAGTGGATGTTTACAAGAGTTGAAAG CTCAAACAATTTCCACCGGGTTTAAATACAATATATTTCTAACTTTCCTAGTAAGTCCTTCGTCTCTGCTCTGATTCTA AGACCAGGTAGACCAGAGTTTCAAGGATGAACACTCAGCTGGACATAGCTTACTGCTCAAAAAGTTCTACAAGAGTTCA GCAGGCAGGTTTTGCTTTCTCTGTTGCCTGAGATATAAATGAAATGCTGTATTTCATGCTGTCGTCTTCAAGAAAAACA AAACCCACTTTTCATCATCAGCACCACCATTCCTAGACTATGTATAAAACTGCATGAACTCATTAAGCTGATGAATGTC TAAACACGTGATTGGACACAAGGATTTGTTCTTGTTTTGTCTACCAGTTCTCCTGTTTATGAGTACTAGAAGAGGAAAA ACAATGCTGAATGAGATTGCGGAAATCTTAACAACATAAGCCATTCATCATCTAGAAGAACAAAAAAATGTGGAGTACA CTGGCCTACTACTGATGACTTCTTTCAGCTTTGATCTAAAGATGTATTTTATTAAAACTATAATTTAAATATACCATGA AAAATATGCAGCAAACATTAGCTCTTTTCTAAAATAGATTAGCCTCTTTGTCTTAGAAATATTGTACTTCCTAATTATT GGTTTAGAGGGGAAAAAAAAAAAA
Citation	Masuda T, Tsuji H, Taniguchi M, Yagi T, Tessier-Lavigne M, Fujisawa H, Okado N, Shiga T. Differential non-target-derived repulsive signals play a critical role in shaping initial axonal growth of dorsal root ganglion neurons. Dev Biol. 2003 Feb 15;254(2):289-302.
Copyright	Copyright © 2003 Elsevier Science (USA). All rights reserved.
Comments	Insufficient information provided in publication to verify the exact sequence used to synthesize probe. The sequence below was obtained from NCBI (NM_204977).

Stage

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Location (click to highlight)

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13-18

stage 14

stage 18

Dermomyotome
Notochord
Surface Ectoderm

Epidermis

19-21

stage 21

Dermatome
Myotome
Notochord
Spinal Cord
Surface Ectoderm

Epidermis

Entry 3

GEISHA Id	Sema3A.Riley.2010	Data Source	Publication
Complete cDNA Template Probe	show GCTGATGGAAGACTTTGTTACTTGTTTCTAGCTGGGAGCGACCCAAAGAGGATTATGTTATGGGCAGAAGATGTTATTT ATGTTGCCTGAAGTTCCCCGGCGGCGCCCGGCAGGACGGGGAGCGCCGCGGCTCCGGCTCGCAGGGACCCTACAGTGGC TGCTGCAGCATGGGCTGGCTCAGGGGGATTGCCTTGCTTTCCTTGGGAGTATTACTAGCAGGAAGAGTGAACTGCCAGC ATGTGAAGAATAACGTGCCAAGACTCAAATTATCCTATAAAGAAATGTTGGAATCCAATAATATAGTCAATTTCAATGG ACTAGCTAATAGTTCCAGTTACCATACTTTCCTCTTGGATGAAGAACGAAGTCGGCTGTATGTTGGAGCAAAAGACCAC ATATTTTCTTTCAACCTGGTTAACATCAAGGAATATCAAAAGATTGTTTGGCCTGTATCTCATTCCCGAAGGGATGAGT GTAAGTGGGCTGGAAAAGATATTCTGAGAGAATGTGCTAATTTCATCAAGGTGCTTAAGACTTATAACCAAACACACTT GTACGCTTGTGGAACGGGGGCTTTTCATCCCATGTGCACTTATATTGAAGTTGGAAGCCATCCTGAGGACAACATTTTT AGGATGGAAGATTCACATTTTGAAAACGGCCGAGGGAAAAGCCCTTACGATCCTAAATTGCTGACAGCTTCTCTTTTAG TAGATGGAGAATTGTACTCTGGCACAGCAGCAGACTTCATGGGCAGAGACTTTGCCATTTTCCGAACTCTGGGGCATCA CCATCCAATCAGAACAGAGCAGCATGACTCCCGGTGGCTAAACGATCCCAGATTTATTAGTGCTCACCTGATACCTGAA AGTGATAATCCAGAAGATGACAAAATCTATTTCTTCTTTCGTGAAAATGCGATTGATGGAGAGCACACTGGAAAAGCCA CTCATGCCAGAATAGGGCAGATCTGTAAGAATGACTTTGGTGGACACAGGAGCCTTGTTAACAAATGGACAACTTTCCT CAAAGCACGTCTGATTTGTTCTGTGCCAGGACCCAATGGTATTGACACACACTTTGATGAATTACAGGACGTGTTCCTA ATGAACTCTAAGGACCCTAAAAATCCAATAGTGTATGGAGTGTTTACAACTTCCAGTAATATCTTCAAGGGATCAGCAG TATGTATGTACAGCATGACTGATGTGAGGAGGGTATTTCTTGGCCCCTATGCCCACCGAGATGGCCCAAACTACCAGTG GGTTCCCTACCAAGGGCGAGTTCCATATCCACGGCCAGGAACTTGCCCCAGTAAAACATTTGGCGGCTTTGACTCCACC AAGGACCTTCCTGATGAAGTTATAACATTTGCAAGAAGTCATCCTGCCATGTATAACCCAGTATTCCCCATCAACAGCC GTCCAATCATGATCAAAACGGATGTAGATTACCAGTTCACACAGATAGTAGTAGATCGAGTAGATGCAGAAGATGGTCA ATACGATGTTATGTTCATTGGCACAGATATTGGAACAGTTCTTAAAGTGGTTTCCATTCCTAAGGAGACTTGGCATGAA TTAGAAGAAGTCTTGCTGGAAGAAATGACCGTCTTTCGGGAACCCACTGTTATTTCAGCAATGAAGATTTCCACAAAAC AGCAACAGCTCTACATTGGCTCAGCCACCGGAGTTTCACAGCTTCCTTTACACCGCTGTGATGTCTATGGAAAAGCATG TGCTGAGTGTTGTCTTGCAAGAGACCCCTACTGCGCCTGGGATGGTTCATCTTGCTCACGTTACTTTCCCACTGCTAAG AGACGTACTAGGCGACAAGACATCCGAAATGGAGATCCCCTCACTCACTGCTCAGACCTGCAGCATCATGATAACCCAA GTGGTCAGACCCTGGAGGAAAAGATTATTTATGGAGTAGAGAATAGCAGCACTTTTCTTGAGTGCAGTCCAAAATCTCA ACGTGCCATAGTCTACTGGCAATTCCAGAAGCAAAATGATGACCACAAAGTTGAGATAAAAGTGGATGATCGAATGATC CGGACAGAACAGGGCCTATTGCTACGAAGTCTTCAACGGAGAGACTCTGGTATTTATTTTTGTCATGCTGTGGAGCATG GCTTCATTCAAACTTTGCTCAAAGTGACACTGGAAGTAATTGATACTGACCACTTGGAAGAGCTGCTCCATAAGGAAGA AGACGCCGATGCCTCCAAGACCAAGGATGCTACCAATAGCATGACTCCTAGTCAAAAGATCTGGTATAGAGACTTCATG CAGTTAATCAATCACCCCAATCTCAACACAATGGATGAATTCTGTGAGCAGGTTTGGAAAAGAGACCGCAAGCAGCGCC GGCAAAGGCCTGCCAATGCGCAGGTGAACACAAATAAGTGGAAGCACCTACAAGAGAATAAAAAAGGTAGGAATAGGAG GACCCATGAATTTGAGAGGGCACCACGGAGTGTCTGAGCTACATTACCTCTAGAAACCTCATCTAAGTAGAAACTTGTA TAGACAGATAACTGGAAAAAAAATGCAATATACATGAACTTTTTCATGGCATTAAGTGGATGTTTACAAGAGTTGAAAG CTCAAACAATTTCCACCGGGTTTAAATACAATATATTTCTAACTTTCCTAGTAAGTCCTTCGTCTCTGCTCTGATTCTA AGACCAGGTAGACCAGAGTTTCAAGGATGAACACTCAGCTGGACATAGCTTACTGCTCAAAAAGTTCTACAAGAGTTCA GCAGGCAGGTTTTGCTTTCTCTGTTGCCTGAGATATAAATGAAATGCTGTATTTCATGCTGTCGTCTTCAAGAAAAACA AAACCCACTTTTCATCATCAGCACCACCATTCCTAGACTATGTATAAAACTGCATGAACTCATTAAGCTGATGAATGTC TAAACACGTGATTGGACACAAGGATTTGTTCTTGTTTTGTCTACCAGTTCTCCTGTTTATGAGTACTAGAAGAGGAAAA ACAATGCTGAATGAGATTGCGGAAATCTTAACAACATAAGCCATTCATCATCTAGAAGAACAAAAAAATGTGGAGTACA CTGGCCTACTACTGATGACTTCTTTCAGCTTTGATCTAAAGATGTATTTTATTAAAACTATAATTTAAATATACCATGA AAAATATGCAGCAAACATTAGCTCTTTTCTAAAATAGATTAGCCTCTTTGTCTTAGAAATATTGTACTTCCTAATTATT GGTTTAGAGGGGAAAAAAAAAAAA
Citation	Riley KL, Gledhill S, Schubert FR. Early expression of axon guidance molecules in the embryonic chick mesencephalon and pretectum. Int J Dev Biol. 2010;54(4):743-53.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204977.1).

Stage

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Location (click to highlight)

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13-18

stage 13

stage 17

Midbrain

19-20

stage 20

Midbrain

Entry 4

GEISHA Id	SEMA3A.Kubilus.2010	Data Source	Publication
Complete cDNA Template Probe	show TTCCATATCCACGGCCAGGAACTTGCCCCAGTAAAACATTTGGCGGCTTTGACTCCACCAAGGACCTTCCTGATGAAGT TATAACATTTGCAAGAAGTCATCCTGCCATGTATAACCCAGTATTCCCCATCAACAGCCGTCCAATCATGATCAAAACG GATGTAGATTACCAGTTCACACAGATAGTAGTAGATCGAGTAGATGCAGAAGATGGTCAATACGATGTTATGTTCATTG GCACAGATATTGGAACAGTTCTTAAAGTGGTTTCCATTCCTAAGGAGACTTGGCATGAATTAGAAGAAGTCTTGCTGGA AGAAATGACCGTCTTTCGGGAACCCACTGTTATTTCAGCAATGAAGATTTCCACAAAACAGCAACAGCTCTACATTGGC TCAGCCACCGGAGTTTCACAGCTTCCTTTACACCGCTGTGATGTCTATGGAAAAGCATGTGCTGAGTGTTGTCTTGCAA GAGACCCCTACTGCGCCTGGGATGGTTCATCTTGCTCACGTTACTTTCCCACTGCTAAGAGACGTACTAGGCGACAAGA CATCCGAAATGGAGATCCCCTCACTCACTGCTCAGACCTGCAGCATCATGATAACCCAAGTGGTCAGACCCTGGAGGAA AAGATTATTTATGGAGTAGAGAATAGCAGCACTTTTCTTGAGTGCAGTCCAAAATCTCAACGTGCCATAGTCTACTGGC AATTCCAGAAGCAAAATGATGACCACAAAGTTGAGATAAAAGTGGATGATCGAATGATCCGGACAGAACAGGGCCTATT GCTACGAAGTCTTCAACGGAGAGACTCTGGTATTTATTTTTGTCATGCTGTGGAGCATGGCTTCATTCAAACTTTGCTC AAAGTGACACTGGAAGTAATTGATACTGACCACTTGGAAGAGCTGCTCCATAAGGAAGAAGACGCCGATGCCT
Citation	Kubilus JK, Linsenmayer TF. Developmental guidance of embryonic corneal innervation: roles of Semaphorin3A and Slit2. Dev Biol. 2010 Aug 1;344(1):172-84. Epub 2010 May 18.
Copyright	Copyright © 2011 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204977.1).

Stage

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1-3

stage 1

Eye

31-40

stage 31

stage 40

Eye
Lens

Entry 5

GEISHA Id	SEMA3A.McLennan.2010	Data Source	Publication
Complete cDNA Template Probe	show GCTGATGGAAGACTTTGTTACTTGTTTCTAGCTGGGAGCGACCCAAAGAGGATTATGTTATGGGCAGAAGATGTTATTT ATGTTGCCTGAAGTTCCCCGGCGGCGCCCGGCAGGACGGGGAGCGCCGCGGCTCCGGCTCGCAGGGACCCTACAGTGGC TGCTGCAGCATGGGCTGGCTCAGGGGGATTGCCTTGCTTTCCTTGGGAGTATTACTAGCAGGAAGAGTGAACTGCCAGC ATGTGAAGAATAACGTGCCAAGACTCAAATTATCCTATAAAGAAATGTTGGAATCCAATAATATAGTCAATTTCAATGG ACTAGCTAATAGTTCCAGTTACCATACTTTCCTCTTGGATGAAGAACGAAGTCGGCTGTATGTTGGAGCAAAAGACCAC ATATTTTCTTTCAACCTGGTTAACATCAAGGAATATCAAAAGATTGTTTGGCCTGTATCTCATTCCCGAAGGGATGAGT GTAAGTGGGCTGGAAAAGATATTCTGAGAGAATGTGCTAATTTCATCAAGGTGCTTAAGACTTATAACCAAACACACTT GTACGCTTGTGGAACGGGGGCTTTTCATCCCATGTGCACTTATATTGAAGTTGGAAGCCATCCTGAGGACAACATTTTT AGGATGGAAGATTCACATTTTGAAAACGGCCGAGGGAAAAGCCCTTACGATCCTAAATTGCTGACAGCTTCTCTTTTAG TAGATGGAGAATTGTACTCTGGCACAGCAGCAGACTTCATGGGCAGAGACTTTGCCATTTTCCGAACTCTGGGGCATCA CCATCCAATCAGAACAGAGCAGCATGACTCCCGGTGGCTAAACGATCCCAGATTTATTAGTGCTCACCTGATACCTGAA AGTGATAATCCAGAAGATGACAAAATCTATTTCTTCTTTCGTGAAAATGCGATTGATGGAGAGCACACTGGAAAAGCCA CTCATGCCAGAATAGGGCAGATCTGTAAGAATGACTTTGGTGGACACAGGAGCCTTGTTAACAAATGGACAACTTTCCT CAAAGCACGTCTGATTTGTTCTGTGCCAGGACCCAATGGTATTGACACACACTTTGATGAATTACAGGACGTGTTCCTA ATGAACTCTAAGGACCCTAAAAATCCAATAGTGTATGGAGTGTTTACAACTTCCAGTAATATCTTCAAGGGATCAGCAG TATGTATGTACAGCATGACTGATGTGAGGAGGGTATTTCTTGGCCCCTATGCCCACCGAGATGGCCCAAACTACCAGTG GGTTCCCTACCAAGGGCGAGTTCCATATCCACGGCCAGGAACTTGCCCCAGTAAAACATTTGGCGGCTTTGACTCCACC AAGGACCTTCCTGATGAAGTTATAACATTTGCAAGAAGTCATCCTGCCATGTATAACCCAGTATTCCCCATCAACAGCC GTCCAATCATGATCAAAACGGATGTAGATTACCAGTTCACACAGATAGTAGTAGATCGAGTAGATGCAGAAGATGGTCA ATACGATGTTATGTTCATTGGCACAGATATTGGAACAGTTCTTAAAGTGGTTTCCATTCCTAAGGAGACTTGGCATGAA TTAGAAGAAGTCTTGCTGGAAGAAATGACCGTCTTTCGGGAACCCACTGTTATTTCAGCAATGAAGATTTCCACAAAAC AGCAACAGCTCTACATTGGCTCAGCCACCGGAGTTTCACAGCTTCCTTTACACCGCTGTGATGTCTATGGAAAAGCATG TGCTGAGTGTTGTCTTGCAAGAGACCCCTACTGCGCCTGGGATGGTTCATCTTGCTCACGTTACTTTCCCACTGCTAAG AGACGTACTAGGCGACAAGACATCCGAAATGGAGATCCCCTCACTCACTGCTCAGACCTGCAGCATCATGATAACCCAA GTGGTCAGACCCTGGAGGAAAAGATTATTTATGGAGTAGAGAATAGCAGCACTTTTCTTGAGTGCAGTCCAAAATCTCA ACGTGCCATAGTCTACTGGCAATTCCAGAAGCAAAATGATGACCACAAAGTTGAGATAAAAGTGGATGATCGAATGATC CGGACAGAACAGGGCCTATTGCTACGAAGTCTTCAACGGAGAGACTCTGGTATTTATTTTTGTCATGCTGTGGAGCATG GCTTCATTCAAACTTTGCTCAAAGTGACACTGGAAGTAATTGATACTGACCACTTGGAAGAGCTGCTCCATAAGGAAGA AGACGCCGATGCCTCCAAGACCAAGGATGCTACCAATAGCATGACTCCTAGTCAAAAGATCTGGTATAGAGACTTCATG CAGTTAATCAATCACCCCAATCTCAACACAATGGATGAATTCTGTGAGCAGGTTTGGAAAAGAGACCGCAAGCAGCGCC GGCAAAGGCCTGCCAATGCGCAGGTGAACACAAATAAGTGGAAGCACCTACAAGAGAATAAAAAAGGTAGGAATAGGAG GACCCATGAATTTGAGAGGGCACCACGGAGTGTCTGAGCTACATTACCTCTAGAAACCTCATCTAAGTAGAAACTTGTA TAGACAGATAACTGGAAAAAAAATGCAATATACATGAACTTTTTCATGGCATTAAGTGGATGTTTACAAGAGTTGAAAG CTCAAACAATTTCCACCGGGTTTAAATACAATATATTTCTAACTTTCCTAGTAAGTCCTTCGTCTCTGCTCTGATTCTA AGACCAGGTAGACCAGAGTTTCAAGGATGAACACTCAGCTGGACATAGCTTACTGCTCAAAAAGTTCTACAAGAGTTCA GCAGGCAGGTTTTGCTTTCTCTGTTGCCTGAGATATAAATGAAATGCTGTATTTCATGCTGTCGTCTTCAAGAAAAACA AAACCCACTTTTCATCATCAGCACCACCATTCCTAGACTATGTATAAAACTGCATGAACTCATTAAGCTGATGAATGTC TAAACACGTGATTGGACACAAGGATTTGTTCTTGTTTTGTCTACCAGTTCTCCTGTTTATGAGTACTAGAAGAGGAAAA ACAATGCTGAATGAGATTGCGGAAATCTTAACAACATAAGCCATTCATCATCTAGAAGAACAAAAAAATGTGGAGTACA CTGGCCTACTACTGATGACTTCTTTCAGCTTTGATCTAAAGATGTATTTTATTAAAACTATAATTTAAATATACCATGA AAAATATGCAGCAAACATTAGCTCTTTTCTAAAATAGATTAGCCTCTTTGTCTTAGAAATATTGTACTTCCTAATTATT GGTTTAGAGGGGAAAAAAAAAAAA
Citation	McLennan R, Teddy JM, Kasemeier-Kulesa JC, Romine MH, Kulesa PM., Vascular endothelial growth factor (VEGF) regulates cranial neural crest migration in vivo. Dev Biol. 2010 Mar 1;339(1):114-25. Epub 2009 Dec 28.
Copyright	Copyright © 2009 Elsevier Inc. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204977.1 ).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

13-16

stage 14

stage 16

Dermomyotome
Notochord

Entry 6

GEISHA Id	SEMA3A.Masuda.2012	Data Source	Publication
Complete cDNA Template Probe	show GCTGATGGAAGACTTTGTTACTTGTTTCTAGCTGGGAGCGACCCAAAGAGGATTATGTTATGGGCAGAAGATGTTATTT ATGTTGCCTGAAGTTCCCCGGCGGCGCCCGGCAGGACGGGGAGCGCCGCGGCTCCGGCTCGCAGGGACCCTACAGTGGC TGCTGCAGCATGGGCTGGCTCAGGGGGATTGCCTTGCTTTCCTTGGGAGTATTACTAGCAGGAAGAGTGAACTGCCAGC ATGTGAAGAATAACGTGCCAAGACTCAAATTATCCTATAAAGAAATGTTGGAATCCAATAATATAGTCAATTTCAATGG ACTAGCTAATAGTTCCAGTTACCATACTTTCCTCTTGGATGAAGAACGAAGTCGGCTGTATGTTGGAGCAAAAGACCAC ATATTTTCTTTCAACCTGGTTAACATCAAGGAATATCAAAAGATTGTTTGGCCTGTATCTCATTCCCGAAGGGATGAGT GTAAGTGGGCTGGAAAAGATATTCTGAGAGAATGTGCTAATTTCATCAAGGTGCTTAAGACTTATAACCAAACACACTT GTACGCTTGTGGAACGGGGGCTTTTCATCCCATGTGCACTTATATTGAAGTTGGAAGCCATCCTGAGGACAACATTTTT AGGATGGAAGATTCACATTTTGAAAACGGCCGAGGGAAAAGCCCTTACGATCCTAAATTGCTGACAGCTTCTCTTTTAG TAGATGGAGAATTGTACTCTGGCACAGCAGCAGACTTCATGGGCAGAGACTTTGCCATTTTCCGAACTCTGGGGCATCA CCATCCAATCAGAACAGAGCAGCATGACTCCCGGTGGCTAAACGATCCCAGATTTATTAGTGCTCACCTGATACCTGAA AGTGATAATCCAGAAGATGACAAAATCTATTTCTTCTTTCGTGAAAATGCGATTGATGGAGAGCACACTGGAAAAGCCA CTCATGCCAGAATAGGGCAGATCTGTAAGAATGACTTTGGTGGACACAGGAGCCTTGTTAACAAATGGACAACTTTCCT CAAAGCACGTCTGATTTGTTCTGTGCCAGGACCCAATGGTATTGACACACACTTTGATGAATTACAGGACGTGTTCCTA ATGAACTCTAAGGACCCTAAAAATCCAATAGTGTATGGAGTGTTTACAACTTCCAGTAATATCTTCAAGGGATCAGCAG TATGTATGTACAGCATGACTGATGTGAGGAGGGTATTTCTTGGCCCCTATGCCCACCGAGATGGCCCAAACTACCAGTG GGTTCCCTACCAAGGGCGAGTTCCATATCCACGGCCAGGAACTTGCCCCAGTAAAACATTTGGCGGCTTTGACTCCACC AAGGACCTTCCTGATGAAGTTATAACATTTGCAAGAAGTCATCCTGCCATGTATAACCCAGTATTCCCCATCAACAGCC GTCCAATCATGATCAAAACGGATGTAGATTACCAGTTCACACAGATAGTAGTAGATCGAGTAGATGCAGAAGATGGTCA ATACGATGTTATGTTCATTGGCACAGATATTGGAACAGTTCTTAAAGTGGTTTCCATTCCTAAGGAGACTTGGCATGAA TTAGAAGAAGTCTTGCTGGAAGAAATGACCGTCTTTCGGGAACCCACTGTTATTTCAGCAATGAAGATTTCCACAAAAC AGCAACAGCTCTACATTGGCTCAGCCACCGGAGTTTCACAGCTTCCTTTACACCGCTGTGATGTCTATGGAAAAGCATG TGCTGAGTGTTGTCTTGCAAGAGACCCCTACTGCGCCTGGGATGGTTCATCTTGCTCACGTTACTTTCCCACTGCTAAG AGACGTACTAGGCGACAAGACATCCGAAATGGAGATCCCCTCACTCACTGCTCAGACCTGCAGCATCATGATAACCCAA GTGGTCAGACCCTGGAGGAAAAGATTATTTATGGAGTAGAGAATAGCAGCACTTTTCTTGAGTGCAGTCCAAAATCTCA ACGTGCCATAGTCTACTGGCAATTCCAGAAGCAAAATGATGACCACAAAGTTGAGATAAAAGTGGATGATCGAATGATC CGGACAGAACAGGGCCTATTGCTACGAAGTCTTCAACGGAGAGACTCTGGTATTTATTTTTGTCATGCTGTGGAGCATG GCTTCATTCAAACTTTGCTCAAAGTGACACTGGAAGTAATTGATACTGACCACTTGGAAGAGCTGCTCCATAAGGAAGA AGACGCCGATGCCTCCAAGACCAAGGATGCTACCAATAGCATGACTCCTAGTCAAAAGATCTGGTATAGAGACTTCATG CAGTTAATCAATCACCCCAATCTCAACACAATGGATGAATTCTGTGAGCAGGTTTGGAAAAGAGACCGCAAGCAGCGCC GGCAAAGGCCTGCCAATGCGCAGGTGAACACAAATAAGTGGAAGCACCTACAAGAGAATAAAAAAGGTAGGAATAGGAG GACCCATGAATTTGAGAGGGCACCACGGAGTGTCTGAGCTACATTACCTCTAGAAACCTCATCTAAGTAGAAACTTGTA TAGACAGATAACTGGAAAAAAAATGCAATATACATGAACTTTTTCATGGCATTAAGTGGATGTTTACAAGAGTTGAAAG CTCAAACAATTTCCACCGGGTTTAAATACAATATATTTCTAACTTTCCTAGTAAGTCCTTCGTCTCTGCTCTGATTCTA AGACCAGGTAGACCAGAGTTTCAAGGATGAACACTCAGCTGGACATAGCTTACTGCTCAAAAAGTTCTACAAGAGTTCA GCAGGCAGGTTTTGCTTTCTCTGTTGCCTGAGATATAAATGAAATGCTGTATTTCATGCTGTCGTCTTCAAGAAAAACA AAACCCACTTTTCATCATCAGCACCACCATTCCTAGACTATGTATAAAACTGCATGAACTCATTAAGCTGATGAATGTC TAAACACGTGATTGGACACAAGGATTTGTTCTTGTTTTGTCTACCAGTTCTCCTGTTTATGAGTACTAGAAGAGGAAAA ACAATGCTGAATGAGATTGCGGAAATCTTAACAACATAAGCCATTCATCATCTAGAAGAACAAAAAAATGTGGAGTACA CTGGCCTACTACTGATGACTTCTTTCAGCTTTGATCTAAAGATGTATTTTATTAAAACTATAATTTAAATATACCATGA AAAATATGCAGCAAACATTAGCTCTTTTCTAAAATAGATTAGCCTCTTTGTCTTAGAAATATTGTACTTCCTAATTATT GGTTTAGAGGGGAAAAAAAAAAAA
Citation	Masuda T, Sakuma C, Taniguchi M, Kanemoto A, Yoshizawa M, Satomi K, Development of the dorsal ramus of the spinal nerve in the chick embryo: a close relationship between development and expression of guidance cues. Brain Res. 2012 Oct 22;1480:30-40. .
Copyright	Copyright © 2012 Elsevier B.V. All rights reserved
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_204977.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 20

stage 22

Dermomyotome
Myotome
Notochord
Spinal Cord
Spinal Ganglia and Nerves

anti-Islet-1/2 (brown)
anti-MF-20 (brown)
anti-MF20 (green)
in situ: SEMA3A (magenta)
in situ: SEMA3A(blue)

25-29

stage 26

stage 29

Dermomyotome
Myotome
Notochord
Spinal Cord
Spinal Ganglia and Nerves

anti-Islet-1/2 (brown)
anti-MF-20 (brown)
anti-MF20 (green)
in situ: SEMA3A (magenta)
in situ: SEMA3A(blue)

Entry 7

GEISHA Id	SEMA3A.Ferrario.2012	Data Source	Publication
Complete cDNA Template Probe	show GCTGATGGAAGACTTTGTTACTTGTTTCTAGCTGGGAGCGACCCAAAGAGGATTATGTTATGGGCAGAAGATGTTATTT ATGTTGCCTGAAGTTCCCCGGCGGCGCCCGGCAGGACGGGGAGCGCCGCGGCTCCGGCTCGCAGGGACCCTACAGTGGC TGCTGCAGCATGGGCTGGCTCAGGGGGATTGCCTTGCTTTCCTTGGGAGTATTACTAGCAGGAAGAGTGAACTGCCAGC ATGTGAAGAATAACGTGCCAAGACTCAAATTATCCTATAAAGAAATGTTGGAATCCAATAATATAGTCAATTTCAATGG ACTAGCTAATAGTTCCAGTTACCATACTTTCCTCTTGGATGAAGAACGAAGTCGGCTGTATGTTGGAGCAAAAGACCAC ATATTTTCTTTCAACCTGGTTAACATCAAGGAATATCAAAAGATTGTTTGGCCTGTATCTCATTCCCGAAGGGATGAGT GTAAGTGGGCTGGAAAAGATATTCTGAGAGAATGTGCTAATTTCATCAAGGTGCTTAAGACTTATAACCAAACACACTT GTACGCTTGTGGAACGGGGGCTTTTCATCCCATGTGCACTTATATTGAAGTTGGAAGCCATCCTGAGGACAACATTTTT AGGATGGAAGATTCACATTTTGAAAACGGCCGAGGGAAAAGCCCTTACGATCCTAAATTGCTGACAGCTTCTCTTTTAG TAGATGGAGAATTGTACTCTGGCACAGCAGCAGACTTCATGGGCAGAGACTTTGCCATTTTCCGAACTCTGGGGCATCA CCATCCAATCAGAACAGAGCAGCATGACTCCCGGTGGCTAAACGATCCCAGATTTATTAGTGCTCACCTGATACCTGAA AGTGATAATCCAGAAGATGACAAAATCTATTTCTTCTTTCGTGAAAATGCGATTGATGGAGAGCACACTGGAAAAGCCA CTCATGCCAGAATAGGGCAGATCTGTAAGAATGACTTTGGTGGACACAGGAGCCTTGTTAACAAATGGACAACTTTCCT CAAAGCACGTCTGATTTGTTCTGTGCCAGGACCCAATGGTATTGACACACACTTTGATGAATTACAGGACGTGTTCCTA ATGAACTCTAAGGACCCTAAAAATCCAATAGTGTATGGAGTGTTTACAACTTCCAGTAATATCTTCAAGGGATCAGCAG TATGTATGTACAGCATGACTGATGTGAGGAGGGTATTTCTTGGCCCCTATGCCCACCGAGATGGCCCAAACTACCAGTG GGTTCCCTACCAAGGGCGAGTTCCATATCCACGGCCAGGAACTTGCCCCAGTAAAACATTTGGCGGCTTTGACTCCACC AAGGACCTTCCTGATGAAGTTATAACATTTGCAAGAAGTCATCCTGCCATGTATAACCCAGTATTCCCCATCAACAGCC GTCCAATCATGATCAAAACGGATGTAGATTACCAGTTCACACAGATAGTAGTAGATCGAGTAGATGCAGAAGATGGTCA ATACGATGTTATGTTCATTGGCACAGATATTGGAACAGTTCTTAAAGTGGTTTCCATTCCTAAGGAGACTTGGCATGAA TTAGAAGAAGTCTTGCTGGAAGAAATGACCGTCTTTCGGGAACCCACTGTTATTTCAGCAATGAAGATTTCCACAAAAC AGCAACAGCTCTACATTGGCTCAGCCACCGGAGTTTCACAGCTTCCTTTACACCGCTGTGATGTCTATGGAAAAGCATG TGCTGAGTGTTGTCTTGCAAGAGACCCCTACTGCGCCTGGGATGGTTCATCTTGCTCACGTTACTTTCCCACTGCTAAG AGACGTACTAGGCGACAAGACATCCGAAATGGAGATCCCCTCACTCACTGCTCAGACCTGCAGCATCATGATAACCCAA GTGGTCAGACCCTGGAGGAAAAGATTATTTATGGAGTAGAGAATAGCAGCACTTTTCTTGAGTGCAGTCCAAAATCTCA ACGTGCCATAGTCTACTGGCAATTCCAGAAGCAAAATGATGACCACAAAGTTGAGATAAAAGTGGATGATCGAATGATC CGGACAGAACAGGGCCTATTGCTACGAAGTCTTCAACGGAGAGACTCTGGTATTTATTTTTGTCATGCTGTGGAGCATG GCTTCATTCAAACTTTGCTCAAAGTGACACTGGAAGTAATTGATACTGACCACTTGGAAGAGCTGCTCCATAAGGAAGA AGACGCCGATGCCTCCAAGACCAAGGATGCTACCAATAGCATGACTCCTAGTCAAAAGATCTGGTATAGAGACTTCATG CAGTTAATCAATCACCCCAATCTCAACACAATGGATGAATTCTGTGAGCAGGTTTGGAAAAGAGACCGCAAGCAGCGCC GGCAAAGGCCTGCCAATGCGCAGGTGAACACAAATAAGTGGAAGCACCTACAAGAGAATAAAAAAGGTAGGAATAGGAG GACCCATGAATTTGAGAGGGCACCACGGAGTGTCTGAGCTACATTACCTCTAGAAACCTCATCTAAGTAGAAACTTGTA TAGACAGATAACTGGAAAAAAAATGCAATATACATGAACTTTTTCATGGCATTAAGTGGATGTTTACAAGAGTTGAAAG CTCAAACAATTTCCACCGGGTTTAAATACAATATATTTCTAACTTTCCTAGTAAGTCCTTCGTCTCTGCTCTGATTCTA AGACCAGGTAGACCAGAGTTTCAAGGATGAACACTCAGCTGGACATAGCTTACTGCTCAAAAAGTTCTACAAGAGTTCA GCAGGCAGGTTTTGCTTTCTCTGTTGCCTGAGATATAAATGAAATGCTGTATTTCATGCTGTCGTCTTCAAGAAAAACA AAACCCACTTTTCATCATCAGCACCACCATTCCTAGACTATGTATAAAACTGCATGAACTCATTAAGCTGATGAATGTC TAAACACGTGATTGGACACAAGGATTTGTTCTTGTTTTGTCTACCAGTTCTCCTGTTTATGAGTACTAGAAGAGGAAAA ACAATGCTGAATGAGATTGCGGAAATCTTAACAACATAAGCCATTCATCATCTAGAAGAACAAAAAAATGTGGAGTACA CTGGCCTACTACTGATGACTTCTTTCAGCTTTGATCTAAAGATGTATTTTATTAAAACTATAATTTAAATATACCATGA AAAATATGCAGCAAACATTAGCTCTTTTCTAAAATAGATTAGCCTCTTTGTCTTAGAAATATTGTACTTCCTAATTATT GGTTTAGAGGGGAAAAAAAAAAAA
Citation	Ferrario JE, Baskaran P, Clark C, Hendry A, Lerner O, Hintze M, Allen J, Chilton JK, Guthrie S. Axon guidance in the developing ocular motor system and Duane retraction syndrome depends on Semaphorin signaling via alpha2-chimaerin. Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14669-74.
Copyright	Copyright © 2012 National Academy of Sciences.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204977.1).