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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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LBX1
ladybird homeobox 1

Gene

Official Symbol	LBX1
Official Full Name	ladybird homeobox 1
CGNC ID	61460
also known as	transcription factor LBX1
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_001178143
Polypeptide	NP_001171614
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.47475

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	100431103
Ensembl Gene
KEGG	gga:100431103

Entry 1

GEISHA Id	LBX1.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	CAAACCCCTCACCCCTTTCAG
Reverse cDNA Template Primer	AATTAACCCTCACTAAAGGCCTTCATCTCCTCCAGGTCG	cDNA Template Length	477
Forward cDNA Template Probe	show `CAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTTACGCGCTGTGCGGGACG GCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGCGCTGCTCTCGCAGACGT CGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTGCTGCAGGCGGCCGAAGG AAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGACGGCCTTCACCAACCAC CAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGACCAGATCGCCCAGCAGC TGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACG`

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Primitive Streak

4-6

stage 4

stage 5

Area Pellucida
Epiblast
Primitive Streak

7-12

stage 7

stage 9

stage 10

stage 11

Anterior Intestinal Portal
Extraembryonic Mesoderm
Forebrain
Headfold
Hindbrain
Intermediate Mesoderm
Lateral Plate Mesoderm
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak
Somites

13-18

stage 14

stage 15

stage 16

Ear/Otic Placode
Forebrain
Heart
Hindbrain
Midbrain
Neural Plate/Tube
Notochord
Renal
Sclerotome
Somatic Mesoderm
Somites

19-24

stage 20

stage 23

Head Mesenchyme
Leg Mesenchyme
Somatic Mesoderm
Spinal Cord
Wing Mesenchyme

stage 25

Head Mesenchyme
Leg Mesenchyme
Somatic Mesoderm
Spinal Cord
Wing Mesenchyme

Entry 2

GEISHA Id	Lbx1.Noden.2006	Data Source	Publication
Complete cDNA Template Probe	show TGCCACCATGACTTCCAAAGAAGAAGCCAAGCCTTCCTCGGGGGAAGAACGACGGAGGAGCCCTTTGGATCACCTCCCG CCTCCGGCTAACTCCAACAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTT ACGCGCTGTGCGGGACGGCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGC GCTGCTCTCGCAGACGTCGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTG CTGCAGGCGGCCGAAGGAAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGA CGGCCTTCACCAACCACCAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGA CCAGATCGCCCAGCAGCTGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACGC GACCTGGAGGAGATGAAGGCCGACGTGGAGTCGGCCAAGAAGCTGGGCCCCAACCCCGCCGTGGACATCGCGGCCTTGG CCGAGCTGGAGCCCAGCGCCGAGGGTCGGGGCAAAGCGCGGCCCGGTTCCCCGCCGCCGGGAAGCGAGGAGGAGGAGGA CGAGGAGGAGGAGGACGTGGAGATCGACGTGGATGACTGAGGGGCGGC
Citation	Noden DM, Francis-West P. The differentiation and morphogenesis of craniofacial muscles. Dev Dyn. 2006 May;235(5):1194-218. Review.
Copyright	Copyright © 2006 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001178143).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-16

stage 16

Face Mesenchyme
Myotome
Somites

Entry 3

GEISHA Id	LBX1.Bothe.2007	Data Source	Publication
Complete cDNA Template Probe	show TGCCACCATGACTTCCAAAGAAGAAGCCAAGCCTTCCTCGGGGGAAGAACGACGGAGGAGCCCTTTGGATCACCTCCCG CCTCCGGCTAACTCCAACAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTT ACGCGCTGTGCGGGACGGCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGC GCTGCTCTCGCAGACGTCGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTG CTGCAGGCGGCCGAAGGAAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGA CGGCCTTCACCAACCACCAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGA CCAGATCGCCCAGCAGCTGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACGC GACCTGGAGGAGATGAAGGCCGACGTGGAGTCGGCCAAGAAGCTGGGCCCCAACCCCGCCGTGGACATCGCGGCCTTGG CCGAGCTGGAGCCCAGCGCCGAGGGTCGGGGCAAAGCGCGGCCCGGTTCCCCGCCGCCGGGAAGCGAGGAGGAGGAGGA CGAGGAGGAGGAGGACGTGGAGATCGACGTGGATGACTGAGGGGCGGC
Citation	Bothe I, Ahmed MU, Winterbottom FL, von Scheven G, Dietrich S. Extrinsic versus intrinsic cues in avian paraxial mesoderm patterning and differentiation. Dev Dyn. 2007 Sep;236(9):2397-409.
Copyright	Copyright © 2007 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001178143).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Dermatome
Myotome

Dermomyotome

Entry 4

GEISHA Id	Lbx1.Bonafede.2006	Data Source	Publication
Complete cDNA Template Probe	show TGCCACCATGACTTCCAAAGAAGAAGCCAAGCCTTCCTCGGGGGAAGAACGACGGAGGAGCCCTTTGGATCACCTCCCG CCTCCGGCTAACTCCAACAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTT ACGCGCTGTGCGGGACGGCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGC GCTGCTCTCGCAGACGTCGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTG CTGCAGGCGGCCGAAGGAAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGA CGGCCTTCACCAACCACCAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGA CCAGATCGCCCAGCAGCTGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACGC GACCTGGAGGAGATGAAGGCCGACGTGGAGTCGGCCAAGAAGCTGGGCCCCAACCCCGCCGTGGACATCGCGGCCTTGG CCGAGCTGGAGCCCAGCGCCGAGGGTCGGGGCAAAGCGCGGCCCGGTTCCCCGCCGCCGGGAAGCGAGGAGGAGGAGGA CGAGGAGGAGGAGGACGTGGAGATCGACGTGGATGACTGAGGGGCGGC
Citation	Bonafede A, Köhler T, Rodriguez-Niedenführ M, Brand-Saberi B. BMPs restrict the position of premuscle masses in the limb buds by influencing Tcf4 expression. Dev Biol. 2006 Nov 15;299(2):330-44. Epub 2006 Jun 15.
Copyright	Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	The following probes were used: Sf/Hgf (Thery et al., 1995), qPax3, MyoD and Myf5, Lbx1, Bmp2 and Bmp4, Tcf4. Probes used in this study were kindly provided by Claudio Stern, Gabrielle Kardon, Christophe Marcelle, Bruce M. Paterson, Susanne Dietrich and Paul Brickell. Thus insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_001178143).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Wing
Wing Muscles

premuscle masses

Entry 5

GEISHA Id	LBX1.Kanamoto.2006	Data Source	Publication
Complete cDNA Template Probe	show TGCCACCATGACTTCCAAAGAAGAAGCCAAGCCTTCCTCGGGGGAAGAACGACGGAGGAGCCCTTTGGATCACCTCCCG CCTCCGGCTAACTCCAACAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTT ACGCGCTGTGCGGGACGGCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGC GCTGCTCTCGCAGACGTCGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTG CTGCAGGCGGCCGAAGGAAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGA CGGCCTTCACCAACCACCAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGA CCAGATCGCCCAGCAGCTGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACGC GACCTGGAGGAGATGAAGGCCGACGTGGAGTCGGCCAAGAAGCTGGGCCCCAACCCCGCCGTGGACATCGCGGCCTTGG CCGAGCTGGAGCCCAGCGCCGAGGGTCGGGGCAAAGCGCGGCCCGGTTCCCCGCCGCCGGGAAGCGAGGAGGAGGAGGA CGAGGAGGAGGAGGACGTGGAGATCGACGTGGATGACTGAGGGGCGGC
Citation	Kanamoto T, Terada K, Yoshikawa H, Furukawa T. Cloning and expression pattern of lbx3, a novel chick homeobox gene. Gene Expr Patterns. 2006 Mar;6(3):241-6. Epub 2005 Dec 27.
Copyright	Reprinted with permission from Elsevier. Copyright © 2005 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001178143).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 16

stage 18

Dermomyotome
Leg Mesenchyme
Somites
Wing Mesenchyme

19-24

stage 19

stage 20

stage 22

stage 23

stage 24

Face Mesenchyme
Limb Buds
Neural Plate/Tube
Somites
Spinal Cord
Wing Mesenchyme

25-28

stage 25

stage 28

Hindbrain
Limb Buds
Neural Plate/Tube
Spinal Cord

Figure 4 includes HH stages 16-28 and compares Lbx1 expression to Lbx3, MyoD, and Myogenin.

Entry 6

GEISHA Id	Lbx1.Christ.2002	Data Source	Publication
Complete cDNA Template Probe	show TGCCACCATGACTTCCAAAGAAGAAGCCAAGCCTTCCTCGGGGGAAGAACGACGGAGGAGCCCTTTGGATCACCTCCCG CCTCCGGCTAACTCCAACAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTT ACGCGCTGTGCGGGACGGCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGC GCTGCTCTCGCAGACGTCGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTG CTGCAGGCGGCCGAAGGAAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGA CGGCCTTCACCAACCACCAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGA CCAGATCGCCCAGCAGCTGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACGC GACCTGGAGGAGATGAAGGCCGACGTGGAGTCGGCCAAGAAGCTGGGCCCCAACCCCGCCGTGGACATCGCGGCCTTGG CCGAGCTGGAGCCCAGCGCCGAGGGTCGGGGCAAAGCGCGGCCCGGTTCCCCGCCGCCGGGAAGCGAGGAGGAGGAGGA CGAGGAGGAGGAGGACGTGGAGATCGACGTGGATGACTGAGGGGCGGC
Citation	Christ B, Brand-Saberi B. Limb muscle development. Int J Dev Biol. 2002;46(7):905-14.
Copyright	"Reprinted from Int. J. Dev. Biol., 46, Christ B, Brand-Saberi B, Limb muscle development, 905-14, Copyright (2002), with permission from Copyright © 2002 UBC Press". All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_001178143).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 21

Leg Muscles
Wing Muscles

31-44

stage 44

Leg Muscles
Somites
Wing Muscles

Comparison to other genes

Entry 7

GEISHA Id	LBX1.Wilm.2004	Data Source	Publication
Complete cDNA Template Probe	show TGCCACCATGACTTCCAAAGAAGAAGCCAAGCCTTCCTCGGGGGAAGAACGACGGAGGAGCCCTTTGGATCACCTCCCG CCTCCGGCTAACTCCAACAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTT ACGCGCTGTGCGGGACGGCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGC GCTGCTCTCGCAGACGTCGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTG CTGCAGGCGGCCGAAGGAAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGA CGGCCTTCACCAACCACCAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGA CCAGATCGCCCAGCAGCTGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACGC GACCTGGAGGAGATGAAGGCCGACGTGGAGTCGGCCAAGAAGCTGGGCCCCAACCCCGCCGTGGACATCGCGGCCTTGG CCGAGCTGGAGCCCAGCGCCGAGGGTCGGGGCAAAGCGCGGCCCGGTTCCCCGCCGCCGGGAAGCGAGGAGGAGGAGGA CGAGGAGGAGGAGGACGTGGAGATCGACGTGGATGACTGAGGGGCGGC
Citation	Wilm B, James RG, Schultheiss TM, Hogan BL (2004). The forkhead genes, Foxc1 and Foxc2, regulate paraxial versus intermediate mesoderm cell fate. Dev Biol. 2004 Jul 1;271(1):176-89.
Copyright	Copyright © 2004 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_001178143).

Stage

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Location (click to highlight)

Comments (click to highlight)

31-35

stage 35

Dermomyotome
Somites

Entry 8

GEISHA Id	LBX1.Alvarez.2003	Data Source	Publication
Complete cDNA Template Probe	show TGCCACCATGACTTCCAAAGAAGAAGCCAAGCCTTCCTCGGGGGAAGAACGACGGAGGAGCCCTTTGGATCACCTCCCG CCTCCGGCTAACTCCAACAAACCCCTCACCCCTTTCAGCATCGAGGACATCCTCAACAAGCCGTCGGTGCGGAGGAGTT ACGCGCTGTGCGGGACGGCCCACCTGCTGTCCGCCGCCGAGAAGCACCCCGCGGCCGGGCTGCCGCTGTCGGGCCGGGC GCTGCTCTCGCAGACGTCGCCGCTGTGCGCCCTGGAGGAGCTGGCCAGCAAAACCTTCAAGGGGCTGGAAGTCAGCGTG CTGCAGGCGGCCGAAGGAAGGGACGGGATGACGATCTTCGGGCAGCGGCAGGCGCCGAAGAAGCGTCGGAAGTCTCGGA CGGCCTTCACCAACCACCAGATCTACGAGCTGGAGAAGCGTTTCCTCTACCAAAAATACCTGTCGCCGGCGGACCGGGA CCAGATCGCCCAGCAGCTGGGGCTCACCAACGCCCAGGTCATCACCTGGTTCCAGAACCGCCGCGCCAAGCTCAAACGC GACCTGGAGGAGATGAAGGCCGACGTGGAGTCGGCCAAGAAGCTGGGCCCCAACCCCGCCGTGGACATCGCGGCCTTGG CCGAGCTGGAGCCCAGCGCCGAGGGTCGGGGCAAAGCGCGGCCCGGTTCCCCGCCGCCGGGAAGCGAGGAGGAGGAGGA CGAGGAGGAGGAGGACGTGGAGATCGACGTGGATGACTGAGGGGCGGC
Citation	Alvares LE, Schubert FR, Thorpe C, Mootoosamy RC, Cheng L, Parkyn G, Lumsden A, Dietrich S. Intrinsic, Hox-dependent cues determine the fate of skeletal muscle precursors. Dev Cell. 2003 Sep;5(3):379-90.
Copyright	Copyright © 2012 Elsevier B.V. All rights reserved.
Comments	Degenerate primers CAGVHMYCGMACRDCYTTYAC and YTBYKSHRYTTDGYVCGNGC directed against the first and third helix of homeobox genes were used for the first amplification, followed by a second round using the nested primer pair ATGGGCCCBCAGMTYKAVRDRYTGGA and TAGCGGCCGCDGYVCGSCGRTTYTGRAACC. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_001178143.1 ).