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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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TIMP2
TIMP metallopeptidase inhibitor 2

Gene

Official Symbol	TIMP2
Official Full Name	TIMP metallopeptidase inhibitor 2
CGNC ID	49073
also known as	metalloproteinase inhibitor 2\|TIMP-2\|tissue inhibitor of metalloproteinase 2\|tissue inhibitor of metalloproteinases 2
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204298
Polypeptide	NP_989629
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.2965

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function	metal ion binding, metalloendopeptidase inhibitor activity
Biological Process
Cellular Component	extracellular region

Links to other databases

Entrez Gene	374178
Ensembl Gene	ENSGALG00000027070
KEGG	gga:374178

Entry 1

GEISHA Id	TIMP2.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	GGAACCCCATCAAGCGAATC
Reverse cDNA Template Primer	ATTAACCCTCACTAAAGGTGTCGGCGTGGACCAGTCTAAC	cDNA Template Length	544
Forward cDNA Template Probe	show TCTGCTGGCTTTCTACTGGCTACTGGAATGCGCTCGGTCGGCTTAGGGGTCCTCGATGTCGAGAAACTCCTGCTTCGGG GGGGCCATGCCGCGGTACCAGGCGCACGAGCCGTCGCTCCTCTTGATGCAGGCGTAGTGCTTGGCCTGCCGCCCGCCCA CGATCTTCTCCATCGCCCAGTCTGTCCAGAGGCACTCATCCGAGGAGGAGACGAAGCAGGGGATGGAGAGGCAGCGCGA GATCTTGCACTCGCAGCCCATCTGGTACCGCTGGTTGAGGCTCTTCTTCTGGGTGGGGCTCAGCGAGTCCCAGGTGGCA ACCAGGTCACACAGCGTGATGTGCATCTTGCCATCGCCCTCGGATTTGCCTGCAATCAGGTACTCCTTCTTGCCTCCTG TGTCCAGAGGCTGCCCACACACTGCCGTGGACGGCGCCGTGTAGATGAATTCGATGTCCTGGTCGGGGCCCTTAAACAT CTTGATCTGCTTCACTTCGTACTGGATTCNCTTGAGGGGG

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Area Opaca
Epiblast
Hensen's Node
Notochord
Primitive Streak

7-12

stage 9

Area Opaca
Blood Vessels
Forebrain
Neural Plate/Tube
Sinus Venosus

13-18

stage 13

stage 14

stage 18

Amnion
Extraembryonic Blood Vessels
Eye
Forebrain
Heart
Heart Tube
Intermediate Mesoderm
Midbrain
Neural Crest
Neural Plate/Tube
Proepicardium
Rhombomeres
Sclerotome
Somites
extraembryonic endoderm

19-20

stage 20

Aorta
Ectoderm
Eye
Leg Mesenchyme
Myotome
Notochord
Sclerotome
Wing Mesenchyme

Entry 2

GEISHA Id	TIMP-2.Linask.2005	Data Source	Publication
Complete cDNA Template Probe	show AATTCCGCGCCGCACGATGCCCGGGGCCGCGCTGCCCAGCCTGCTGGCCTGGCTGGCGGTGCTGCTGCTCGGCAGGGCC CGCCCGGCCGACGCGTGCAGCTGCTCGCCCATCCACCCGCAGCAGGCCTTCTGCAACGCCGACGTAGTGATCCGAGCAA AGCGCGTCTCGGCGAAGGAGGTGGATTCAGGGAATGACATTTATGGGAACCCCATCAAGCGAATCCAGTACGAAGTGAA GCAGATCAAGATGTTTAAGGGCCCCGACCAGGACATCGAATTCATCTACACGGCGCCGTCCACCGAGGTGTGTGGGCAG CCTCTGGACACAGGAGGTAAGAAGGAGTACCTGATTGCAGGCAAATCCGAGGGCGATGGCAAGATGCACATCACGCTGT GTGACCTGGTTGCCACCTGGGACTCGGTGAGCCCCACCCAGAAGAAGAGCCTCAACCAGCGGTACCAGATGGGCTGCGA GTGCAAGATCTCGCGCTGCCTCTCCATCCCCTGCTTCGTCTCCTCCTCGGATGAGTGCCTCTGGACAGACTGGGCGATG GAGAAGATCGTGGGCGGGCGGCAGGCCAAGCACTACGCCTGCATCAAGAGGAGCGACGGCTCGTGCGCCTGGTACCGCG GCATGGCCCCCCCGAAGCAGGAGTTTCTCGACATCGAGGACCCCTAAGCCGACCGAGCGCATTCCAGTAGCCAGTAGGA AAGCCCAGCGAGATGTTAGACTGGTCCACGCCGACATCGGGGCCTGGAAAGCATGAAAACCCACACACGCCGAGGGGCC GCAGGGCACGGAGCTGCCGGAATT
Citation	Linask K, Han M, Cai D, Brauer P, Manisastry, S. Cardiac Morphogenesis: Matrix Metalloproteinase Coordination of Cellular Mechanisms Underlying Heart Tube Formation and Directionality of Looping. Dev Dyn. 2005 Jul;233(3):739-53.
Copyright	Copyright © 2005 Wiley-Liss, Inc.
Comments	According to the authors: "Digoxigenin-labeled antisense and sense riboprobes for chicken MMP-2 and TIMP-2 were generated and the hybridization carried out as described in Cai et al. (2000)." Cai does not mention TIMP-2, therefore insufficient information is provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204298).

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 1

Endocardium
Heart
Myocardium
Splanchnic Mesoderm

dorsal mesentery

Entry 3

GEISHA Id	TIMP-2.Brauer.2002	Data Source	Publication
Complete cDNA Template Probe	show AATTCCGCGCCGCACGATGCCCGGGGCCGCGCTGCCCAGCCTGCTGGCCTGGCTGGCGGTGCTGCTGCTCGGCAGGGCC CGCCCGGCCGACGCGTGCAGCTGCTCGCCCATCCACCCGCAGCAGGCCTTCTGCAACGCCGACGTAGTGATCCGAGCAA AGCGCGTCTCGGCGAAGGAGGTGGATTCAGGGAATGACATTTATGGGAACCCCATCAAGCGAATCCAGTACGAAGTGAA GCAGATCAAGATGTTTAAGGGCCCCGACCAGGACATCGAATTCATCTACACGGCGCCGTCCACCGAGGTGTGTGGGCAG CCTCTGGACACAGGAGGTAAGAAGGAGTACCTGATTGCAGGCAAATCCGAGGGCGATGGCAAGATGCACATCACGCTGT GTGACCTGGTTGCCACCTGGGACTCGGTGAGCCCCACCCAGAAGAAGAGCCTCAACCAGCGGTACCAGATGGGCTGCGA GTGCAAGATCTCGCGCTGCCTCTCCATCCCCTGCTTCGTCTCCTCCTCGGATGAGTGCCTCTGGACAGACTGGGCGATG GAGAAGATCGTGGGCGGGCGGCAGGCCAAGCACTACGCCTGCATCAAGAGGAGCGACGGCTCGTGCGCCTGGTACCGCG GCATGGCCCCCCCGAAGCAGGAGTTTCTCGACATCGAGGACCCCTAAGCCGACCGAGCGCATTCCAGTAGCCAGTAGGA AAGCCCAGCGAGATGTTAGACTGGTCCACGCCGACATCGGGGCCTGGAAAGCATGAAAACCCACACACGCCGAGGGGCC GCAGGGCACGGAGCTGCCGGAATT
Citation	Brauer PR, Cai DH. Expression of tissue inhibitor of metalloproteinases (TIMPs) during early cardiac development. Mech Dev. 2002 May;113(2):175-9.
Copyright	Copyright © 2002 Elsevier Science Ireland Ltd.
Comments	The complete cDNA template sequence was obtained from the information in the publication as described in Brauer et al. 2002.