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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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MYOCD
myocardin

Gene

Official Symbol	MYOCD
Official Full Name	myocardin
CGNC ID	685
also known as	myocardin
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_001080715
Polypeptide	NP_001074184
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.37764

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	427790
Ensembl Gene	ENSGALG00000001021
KEGG	gga:427790

Entry 1

GEISHA Id	MYOCD.UAlinear	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CAGCTTTTCTCTTTGGAGGAAGTTAATGGTTTAAGTGAGGGGCGTCTGGATCCGGTCCCCGAGACACCATGACACTCCT GGGGTCTGAGCACTCCTTGCTGATTCGGAGCAAGTTCAGATCAGTTTTGCAGCTACGGCTTCAGCAGAGAAGGACCCGT GAGCAGCTGGCAGACCAAGGCATCATGCCACCACTGAAAAGCCCATCCGCATTCCATGAACAACGAAAAAGTCTGGAGC GAGCCAAGACTGAAGATTATCTCAAGCACAAGATCAGAAGCAGGCCTGAGCGATCAGACCTGGTCAATATGCACATTTT ACAAGACTCAGCTGCAGAGGGGTCCATTCAGTCCACTCAAATGAAGCTGAAAAGAGCCCGACTGGCAGATGATCTCAAT GAAAAGATTGCTCTCAGGCCGGGTCCTTTGGAGCTGGTGGAGAAGAATATTATTCCTGTCGACTCAGCTGTGAAAGAGG CCATAAAAGGCACACAGGTGAACTTCTCCAAGCCACCTGATGCGTTTGCCTTTGAGGAGGACAGCAGCAATGACGGCCT GTCCCCGGAGCAGGCCAGGAGCGAGGACTCCCAGGGCTCCACAGAGTCGTCGGCAGGCACCAAATCCTCCGAGCTGCCG CCCTCGGCCCCCTCAGGGCCGGCCCAGGATCACCCCCATGGCATTGAGGGCAACACAGCGGAGCTTGCCTCAGGCCAGA GCGGCCAGTGCAACAGCCCCAAGCAAGCAGTGGGGCAGGACAGCCCACCTCTCCCAGTGCCCTCCGTGGTGAAGTCAAA ATCATCCAGTGATAGCAAGAACCGTCACAAAAAACCCAAGGACACTAAGCCCAAGGTGAAGAAGCTGAAATATCACCAG TACATCCCTCCAGACCAGAAGGCAGAGAAGTCCCCTCCACCTATGGACTCTGCATATGCAAGACTCCTCCAGCAGCAGC AGCTCTTTCTGCAGCTGCAGATCCTCAGCCAGCAGCAACAGCAACAACAGCAGCAACAGCAGCAACAGCAGCAGCACTT CAACTACCCAGGGATGCACCAAGGCCAGATAAAGCAATCAAATGAGCAAATGATCAAAAGTTCAAATGCTTCATCAACT TCTGTCAGCAACACCCCTCTTTCTCCTGTGAAAACCACCTTTTCAGGCCAGACTTGTGTCTCATCTATCAAGCCAGGCC CTCTGCCATCTAACCTGGATGATCTGAAAGTGTCAGAACTGAGACAGCAGCTCCGAATAAGAGGCCTGCCTGTATCAGG TACCAAAGCAGCACTGATGGAACGGCTACGGCCCTTCCAGGAGTGCAGCAGCAATGCGGTGCCAAATTTCAGTGAGATC ACAACTGTCACCTTTCCAGTCACTCCAACAAGCACCTTGTCAAGTTACCAGTCGCAGTCTTCCACTGGCATGTTATCAA ACGGCTTCTACCACTTCGGCAGCACCAGTTCCACTCCACCCATCTCTCCAGCCTCCTCTGACCTCTCTGTGAGCGGCTC TCTGCCAGACACATTCAACGACGGGCCCATGTCTTCTCCACAGTTTGGTCTTCAGCCATCTCCAGTTCACGGTAGTGCT GAGGAAAGCCTGATGAGCAGCATGAACGGAGGGAGCATCCAGCTGGAGCTGGAAGGGATTGACACAGAGAAGGACAAAA TGCTGGTGGAGAAGCAGAAGGTCATCAATGAATTGACGTGGAAGCTGCAACAAGAGCAGAGGCAGGTAGAAGAACTAAG GATGCAACTGCAGAAGCGAAAGAGAAGCAATGGCCTTGAGGAGAAACAGCAGCCCTCCCAGCATTTCTTTGGTGTTCCC ATCAAGCAAGAAAATGCAGTGTCCAGCTGCCCATTTGCATCCAAACAAACTGCCTTGAAAGGCCAAGCCAGCAGCTCGG ATAAGTTAAGTAACTGTGGGGTGCCACAGCTGTCTCACATTGTAAATAGCCACTGCTTGGAGCCTGCGGGGCAAAGTAC CATCACCTCCTCGACATTCCTGAGCCCGCAGTGCTCCCCCCAGCACTCTCCACTCGGGGCTGCAAAGAGCCCTCAACGC ATCAGCCTGCCACCGTCCCCCAACAGCCACTATCTCCTGGCGGTGTCCCCTGGCTCACAGGCAGAAGGGCGCAGCGCAT CGCCCCAGGCCAGCAGTTGCCTTCGCACGGCATCGCAGATGACTCGAAGTCAGCAGATGGACGAACTCCTGGACGTGCT GATTGAGAGCGGAGAAATGCCAGCCAACGCCAAGGAAGATCGGTCCTGCCTCCAGAAAGTACCTCAGATAACAGTGTCT ACAGGGAACTCCAGCACTCCGCTCCCAAAGTCATCTGCTCCATTCGAACAGGTCTCCTCCACCCAGCTCCCATTCGAGC ACTGTCCAGGCGGTGGTGACGCTCACCTCGAGGTCCTGCTGAATGCCCACAGCCCGCTGGGGAGAGTCGGTGAAATCAC CCTGCTGAAGATGGGAGGAGAGGAGTCCCACTTCGAAGGGATGATAGAGGGGTTCTCCAGCAAAGCCGCAGATGAACTG CTCACCTCCCAGGAGATTTTACAGACTCCCCTCTCACCCATGGAAACGCAGCTCTCCCCTTCCCCTGCTGATGGCTCCG GTTTACAAATGAGTTTCACTGAATCTCCATGGGAAACGATGGAGTGGCTGGACCTCACCCCCCCCAGCTCTGCCACTGG CTTCAGCTCACTCACTCCTGCAGGCCCCAGCATCTTCAACATTGATTTCCTGGATGTTACTGATCTCAATTTAAACACC AGCATGGACCTCCACCTGCAGCAGTGGTGAACAGGAGGGCAGTGGAGGCAGAGTACTGCGAGCCCACAGCAGCCAGCGC AGTGTTTCCGTCATGCTGGAGAA

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

stage 6

Area Opaca
Cardiovascular
Extraembryonic Mesoderm
Lateral Plate Mesoderm
Splanchnic Mesoderm

7-12

stage 7

stage 8

stage 9

Anterior Intestinal Portal
Area Opaca
Cardiovascular
Extraembryonic Mesoderm
Heart
Heart Tube
Lateral Plate Mesoderm
Splanchnic Mesoderm

HH 7+/8-
HH 8+/9-

13-18

stage 13

stage 18

Allantois
Atria
Blood Vessels
Eye
Heart
Myocardium
Outflow Tract
Sinus Venosus
Ventricles
Yolk Sack or Stalk

Entry 2

GEISHA Id	MYOCD.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATAGCAGCAGCACTTCAACTACCC
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGCTTCTCCACCAGCATTTTGTCC	cDNA Template Length	669
Forward cDNA Template Probe	show AGGGGATGCACCAAGGGCCAGATAAAGCAATCAAATGAGCAAATGGATCAAAAGTTCAAATGCTTCATCAACTTCTGTC AGCAACACCCCTCTTTCTCCTGTGAAAACCACCTTTTCAGGCCAGACTTGTGTCTCATCTATCAAGCCAGGCCCTCTGC CATCTAACCTGGATGATCTGAAAGTGTCAGAACTGAGACAGCAGCTCCGAATAAGAGGCCTGCCTGTATCAGGTACCAA AACAGCACTGATGGAACGGCTACGGCCCTTCCAGGAGTGCAGCAGCAATGCGGTGCCAAATTTCAGTGAGATCACAACT GTCACCTTTCCAGTCACTCCAACAAGCACCTTGTCAAGTTACCAGTCGCAGTCTTCCACTGGCATGTTATCAAACGGCT TCTACCACTTCGGCAGCACCAGTTCCACTCCACCCATCTCTCCAGCCTCCTCTGACCTCTCTGTGAGCGGCTCTCTGCC AGACACATTCAACGACGGGCCCATGTCTTCTCCACAGTTTGGTCTTCAGCCATCTCCAGTTCACGGTAGTGCTGAGGAA AGCCTGATGAGCAGCATGAACGGAGGGAGCATCCAGCTGGAGCTGGAAGGGATTGACACAGAGAAGGACAAAATGCTGG TGGAGAAGCCTTTAGTGAGGGTTATTTCTAAGAGCAA
Comments	No expression detected at or before stage 4.

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Unlabeled

No expression detected at or before stage 4.

7-12

stage 11

Blood Vessels
Heart Tube
Outflow Tract
Sinus Venosus

13-18

stage 15

stage 16

Blood Vessels
Outflow Tract
Sinus Venosus

19-24

stage 20

stage 22

stage 24

Extraembryonic
Head Mesenchyme
Heart

Entry 3

GEISHA Id	MYOCARDIN.Chen.2008	Data Source	Publication
Complete cDNA Template Probe	show CAGCTTTTCTCTTTGGAGGAAGTTAATGGTTTAAGTGAGGGGCGTCTGGATCCGGTCCCCGAGACACCATGACACTCCT GGGGTCTGAGCACTCCTTGCTGATTCGGAGCAAGTTCAGATCAGTTTTGCAGCTACGGCTTCAGCAGAGAAGGACCCGT GAGCAGCTGGCAGACCAAGGCATCATGCCACCACTGAAAAGCCCATCCGCATTCCATGAACAACGAAAAAGTCTGGAGC GAGCCAAGACTGAAGATTATCTCAAGCACAAGATCAGAAGCAGGCCTGAGCGATCAGACCTGGTCAATATGCACATTTT ACAAGACTCAGCTGCAGAGGGGTCCATTCAGTCCACTCAAATGAAGCTGAAAAGAGCCCGACTGGCAGATGATCTCAAT GAAAAGATTGCTCTCAGGCCGGGTCCTTTGGAGCTGGTGGAGAAGAATATTATTCCTGTCGACTCAGCTGTGAAAGAGG CCATAAAAGGCACACAGGTGAACTTCTCCAAGCCACCTGATGCGTTTGCCTTTGAGGAGGACAGCAGCAATGACGGCCT GTCCCCGGAGCAGGCCAGGAGCGAGGACTCCCAGGGCTCCACAGAGTCGTCGGCAGGCACCAAATCCTCCGAGCTGCCG CCCTCGGCCCCCTCAGGGCCGGCCCAGGATCACCCCCATGGCATTGAGGGCAACACAGCGGAGCTTGCCTCAGGCCAGA GCGGCCAGTGCAACAGCCCCAAGCAAGCAGTGGGGCAGGACAGCCCACCTCTCCCAGTGCCCTCCGTGGTGAAGTCAAA ATCATCCAGTGATAGCAAGAACCGTCACAAAAAACCCAAGGACACTAAGCCCAAGGTGAAGAAGCTGAAATATCACCAG TACATCCCTCCAGACCAGAAGGCAGAGAAGTCCCCTCCACCTATGGACTCTGCATATGCAAGACTCCTCCAGCAGCAGC AGCTCTTTCTGCAGCTGCAGATCCTCAGCCAGCAGCAACAGCAACAACAGCAGCAACAGCAGCAACAGCAGCAGCACTT CAACTACCCAGGGATGCACCAAGGCCAGATAAAGCAATCAAATGAGCAAATGATCAAAAGTTCAAATGCTTCATCAACT TCTGTCAGCAACACCCCTCTTTCTCCTGTGAAAACCACCTTTTCAGGCCAGACTTGTGTCTCATCTATCAAGCCAGGCC CTCTGCCATCTAACCTGGATGATCTGAAAGTGTCAGAACTGAGACAGCAGCTCCGAATAAGAGGCCTGCCTGTATCAGG TACCAAAGCAGCACTGATGGAACGGCTACGGCCCTTCCAGGAGTGCAGCAGCAATGCGGTGCCAAATTTCAGTGAGATC ACAACTGTCACCTTTCCAGTCACTCCAACAAGCACCTTGTCAAGTTACCAGTCGCAGTCTTCCACTGGCATGTTATCAA ACGGCTTCTACCACTTCGGCAGCACCAGTTCCACTCCACCCATCTCTCCAGCCTCCTCTGACCTCTCTGTGAGCGGCTC TCTGCCAGACACATTCAACGACGGGCCCATGTCTTCTCCACAGTTTGGTCTTCAGCCATCTCCAGTTCACGGTAGTGCT GAGGAAAGCCTGATGAGCAGCATGAACGGAGGGAGCATCCAGCTGGAGCTGGAAGGGATTGACACAGAGAAGGACAAAA TGCTGGTGGAGAAGCAGAAGGTCATCAATGAATTGACGTGGAAGCTGCAACAAGAGCAGAGGCAGGTAGAAGAACTAAG GATGCAACTGCAGAAGCGAAAGAGAAGCAATGGCCTTGAGGAGAAACAGCAGCCCTCCCAGCATTTCTTTGGTGTTCCC ATCAAGCAAGAAAATGCAGTGTCCAGCTGCCCATTTGCATCCAAACAAACTGCCTTGAAAGGCCAAGCCAGCAGCTCGG ATAAGTTAAGTAACTGTGGGGTGCCACAGCTGTCTCACATTGTAAATAGCCACTGCTTGGAGCCTGCGGGGCAAAGTAC CATCACCTCCTCGACATTCCTGAGCCCGCAGTGCTCCCCCCAGCACTCTCCACTCGGGGCTGCAAAGAGCCCTCAACGC ATCAGCCTGCCACCGTCCCCCAACAGCCACTATCTCCTGGCGGTGTCCCCTGGCTCACAGGCAGAAGGGCGCAGCGCAT CGCCCCAGGCCAGCAGTTGCCTTCGCACGGCATCGCAGATGACTCGAAGTCAGCAGATGGACGAACTCCTGGACGTGCT GATTGAGAGCGGAGAAATGCCAGCCAACGCCAAGGAAGATCGGTCCTGCCTCCAGAAAGTACCTCAGATAACAGTGTCT ACAGGGAACTCCAGCACTCCGCTCCCAAAGTCATCTGCTCCATTCGAACAGGTCTCCTCCACCCAGCTCCCATTCGAGC ACTGTCCAGGCGGTGGTGACGCTCACCTCGAGGTCCTGCTGAATGCCCACAGCCCGCTGGGGAGAGTCGGTGAAATCAC CCTGCTGAAGATGGGAGGAGAGGAGTCCCACTTCGAAGGGATGATAGAGGGGTTCTCCAGCAAAGCCGCAGATGAACTG CTCACCTCCCAGGAGATTTTACAGACTCCCCTCTCACCCATGGAAACGCAGCTCTCCCCTTCCCCTGCTGATGGCTCCG GTTTACAAATGAGTTTCACTGAATCTCCATGGGAAACGATGGAGTGGCTGGACCTCACCCCCCCCAGCTCTGCCACTGG CTTCAGCTCACTCACTCCTGCAGGCCCCAGCATCTTCAACATTGATTTCCTGGATGTTACTGATCTCAATTTAAACACC AGCATGGACCTCCACCTGCAGCAGTGGTGAACAGGAGGGCAGTGGAGGCAGAGTACTGCGAGCCCACAGCAGCCAGCGC AGTGTTTCCGTCATGCTGGAGAA
Citation	Chen JF, Wang S, Wu Q, Cao D, Nguyen T, Chen Y, Wang DZ. Myocardin Marks the Earliest Cardiac Gene Expression and Plays an Important Role in Heart Development. Anat Rec (Hoboken). 2008 October; 291(10): 12001211.
Copyright	Copyright © 2008 Wiley-Liss, Inc.
Comments	Authors indicated:Two different cDNA fragments, which cover distinct portions of the chick myocardin transcripts (ORF and 3′ UTR), were used as templates for in vitro transcription to make antisense DIG-labeled riboprobes. These two probes were used to hybridize with staged chick embryos and yielded similar results. The sequence for the total mRNA is included here. NM_001080715.1