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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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TCF21
transcription factor 21

Gene

Official Symbol	TCF21
Official Full Name	transcription factor 21
CGNC ID	10451
also known as	transcription factor 21, Transcription factor 21, Podocyte-expressed 1, Pod-1, Epicardin, Capsulin
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_001277711
Polypeptide	NP_001264640
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.8241

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	6943	ENSG00000118526		MIM:603306
Mouse	21412	ENSMUSG00000045680	All phenotypic alleles (3):Targeted, knock-out(1) Targeted, other(2)	MGI:1202715
Xenopus	100126209, 444386			484804
Zebrafish	558148	ENSDARG00000036869	ZDB-GENE-051113-88	ZFIN:ZDB-GENE-051113-88

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	421697
Ensembl Gene
KEGG	gga:421697

Entry 1

GEISHA Id	ChEST265h24	Data Source	GEISHA ISH Analysis
Forward cDNA Template Probe	show CGGGGTCCTCTCTGGATCTATATCACGCCTTAGTGCCGGAGGGGGGAGAGAAGGGGAGGAAAGTGCTGCGCCCCTTCTC TCCTCCACCGCCACCACGTAGGTTTGGGGAGCTCCCCGTCCTCCCCTCCTCCCCCTTTCCCGCCAGCCCTTTGGCATCT TCCAGACCATGTCCACTGGGTCCCTCAGTGATGTGGAAGATCTGCAAGAGGTGGAAATGCTGGAGTGCGATGGCCTGAA AATGGATACTAACAAAGAGTTCGGGGCGTCCAACGAGAGCAACGAGGAGGGATCCAATGGCGAGAATGGCTCCCCTCAG AAAGGGAGAGGGGCCTCGGGAAAGAGGAAAAAGGCTCCCCCCAAGAAGAGCCCTTTAAATGGTGTGAGCCAGGAAGGAA AGCAGGTCCAGAGAAACGCTGCCAACGCAAGGGAGAGGGCAAGGATGAGGGTCCTTAGCAAAGCCTTCTCCAGGCTTAA GACCACCCTGCCCTGGGTGCCCCCAGACACCAAGCTTTCCAAACTGGACACCTTGAGGCTGGCCTCCAGCTACATCGCA CACCTGAGGCAAATCCTGGCCAACGACAAGTACGAAAATGGCTACATCCATCCAGTCAACCTGACCTGGCCTTTTATGG TAGCCGGCAAACCCGAGAGTGACCTGAAAGAAGTGGTGAACACAAACCGCTTGTGCGGCCCGACGGCATCCTGAGCCCC GCGGCCCCGCCAGCACCGACCCCGTGCGGCCGGGGAGGGGGCACGGGCGCGGGCAGGGCCCCGCTTTGCCCCGCTGCTG CCCCGGGCCCGGGCAGGGCGACGGGAGAGCGCTCCGCCGGCACGGCACGGCGGAGACCGAAGGCAGAGACTCCACCCCG GGAGAGCCCGACTCTATTTAACTTTATTGAGTGCGTGTGCAGTCGAGTGTCCTGCAACAGCAGCCTTGCTGGTTCAAGC GCTACTAGAGGAAAGGCAGAGATCAAACAAAACCTACCACGCGTGTATCTTTTGTCTGAGACCTGTGGAATATGTAGAT GCCTAGAAAAAAAAAAAAAAACTGACTTTGACAGTCATCTCTATGAACTCATTCGCCCTAAAGATATATAGATATATTT TCTTCAAGCAGGTTTTTGCTCTATTTCTGTGAATAAACCTTCCTTTCCATTGAACACG
Comments	No specific expression detected at stage 3.

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 6

Early Mesoderm
Lateral Plate Mesoderm
Splanchnic Mesoderm

heart field

7-12

stage 11

Intermediate Mesoderm

13-18

stage 14

stage 15

stage 16

Intermediate Mesoderm
Proepicardium

19-24

stage 19

stage 20

stage 21

stage 23

stage 24

Allantois
Amnion
Epicardium
Gonad
Gut
Head Mesenchyme
Outflow Tract
Proepicardium
Renal
Sinus Venosus
Splanchnic Mesoderm

hand transected

Entry 2

GEISHA Id	Capsulin.Dastjerdi.2007	Data Source	Publication
Complete cDNA Template Probe	show `CGATGGCCTGAAAATGGATACTAACAAAGAGTTCGGGGCGTCCAACGAGAGCAACGAGGAGGGATCCAATGGCGAGAAT GGCTCCCCTCAGAAAGGGAGAGGGGCCTCGGGAAAGAGGAAAAAGGCTCCCCCCAAGAAGAGCCCTTTAAATGGTGTGA GCCAGGAAGGAAAGCAGGTCCAGAGAAACGCTGCCAACGCAAGGGAGAGGGCAAGGATGAGGGTCCTTAGCAAAGCCTT CTCCAGGCTTAAGACCACCCTGCCCTGGGTGCCCCCAGACACCAAGCTTTCCAAACTGGACACCTTGAGGCTGGCCTCC AGCTACATCGCACACCTGAGGCAAATCCTGGCCAACGACAAGTACGAAAATGGCTACATCCATCCAGTCAACCTGACCT GGCCTTTTATGGTAGCCGGCAAACCCGAGAGTGACCTGAAAGAAGTGGTGAACACAAACCGCTTGTGCGGCCCGACGGC ATCCTGAGCCCCGCGG`
Citation	Dastjerdi A, Robson L, Walker R, Hadley J, Zhang Z, Rodriguez-Niedenführ M, Ataliotis P, Baldini A, Scambler P, Francis-West P. Tbx1 regulation of myogenic differentiation in the limb and cranial mesoderm. Dev Dyn. 2007 Feb;236(2):353-63.
Copyright	Copyright © 2007 Wiley-Liss, Inc.
Comments	According to the authors, "A 550-bp capsulin fragment was PCRed using 5CGA TGC CCT GAA AAT GGA TAC TAA 3', and 5' CCG CGG GGC TCA GGA TGC 3' primers."

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 11

stage 12

Intermediate Mesoderm
Pharyngeal Arches and Clefts

Entry 3

GEISHA Id	Capsulin.Ishii.2007	Data Source	Publication
Complete cDNA Template Probe	show `GGGTCCTCTCTGGATCTATATCACGCCTTAGTGCCGGAGGGGGGAGAGAAGGGGAGGAAAGTGCTGCGCCCCTTCTCTC CTCCACCGCCACCACGTAGGTTTGGGGAGCTCCCCGTCCTCCCCTCCTCCCCCTTTCCCGCCAGCCCTTTGGCATCTTC CAGACCATGTCCACTGGGTCCCTCAGTGATGTGGAAGATCTGCAAGAGGTGGAAATGCTGGAGTGCGATGGCCTGAAAA TGGATACTAACAAAGAGTTCGGGGCGTCCAACGAGAGCAACGAGGAGGGATCCAATGGCGAGAATGGC`
Citation	Ishii Y, Langberg JD, Hurtado R, Lee S, Mikawa T. Induction of proepicardial marker gene expression by the liver bud. Development. 2007 Oct;134(20):3627-37. Epub 2007 Sep 12.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. Permission granted by T. Mikawa.
Comments	Sequence below was obtained from NCBI (BX932800) using the primers given in the publication: chick/quail capsulin (c/qcapsulin) forward primer, 5'-GGGTCCTCTCTGGATCTATATCAC-3' and reverse primer, 5'-GCCATTCTCGCCATTGGAT-3'.

Stage

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Location (click to highlight)

Comments (click to highlight)

13-16

stage 14

stage 16

Intermediate Mesoderm
Proepicardium

Entry 4

GEISHA Id	Capsulin.vonScheven.2006	Data Source	Publication
Complete cDNA Template Probe	show AGAGAAGGGGAGGAAAGTGCTGCGCCCCTTCTCTCCTCCACCGCCACCACGTAGGTTTGGGGAGCTCCCCGTCCTCCCC TCCTCCCCCTTTCCCGCCAGCCCTTTGGCATCTTCCAGACCATGTCCACTGGGTCCCTCAGTGATGTGGAAGATCTGCA AGAGGTGGAAATGCTGGAGTGCGATGGCCTGAAAATGGATACTAACAAAGAGTTCGGGGCGTCCAACGAGAGCAACGAG GAGGGATCCAATGGCGAGAATGGCTCCCCTCAGAAAGGGAGAGGGGCCTCGGGAAAGAGGAAAAAGGCTCCCCCCAAGA AGAGCCCTTTAAATGGTGTGAGCCAGGAAGGAAAGCAGGTCCAGAGAAACGCTGCCAACGCAAGGGAGAGGGCAAGGAT GAGGGTCCTTAGCAAAGCCTTCTCCAGGCTTAAGACCACCCTGCCCTGGGTGCCCCCAGACACCAAGCTTTCCAAACTG GACACCTTGAGGCTGGCCTCCAGCTACATCGCACACCTGAGGCAAATCCTGGCCAACGACAAGTACGAAAATGGCTACA TCCATCCAGTCAACCTGACCTGGCCTTTTATGGTAGCCGGCAAAC
Citation	von Scheven G, Bothe I, Ahmed MU, Alvares LE, Dietrich S (2006). Protein and genomic organisation of vertebrate MyoR and Capsulin genes and their expression during avian development. Gene Expr Patterns. Apr;6(4):383-93. Epub 2006 Jan 18.
Copyright	Copyright © 2005 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # XM_419734).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

stage 12

Allantois
Anterior Intestinal Portal
Aorta
Foregut
Heart
Intermediate Mesoderm
Lateral Plate Mesoderm
Pharyngeal Arches and Clefts
Somatic Mesoderm
Somatopleure
Splanchnic Mesoderm

Coelom

13-18

stage 16

stage 18

Allantois
Anterior Intestinal Portal
Epicardium
Esophagus
Heart
Lateral Plate Mesoderm
Lung
Nephric Tubules
Pharyngeal Arches and Clefts
Proepicardium
Splanchnic Mesoderm

Tracheae

19-22

stage 20

stage 21

stage 22

Allantois
Epicardium
Gut
Lung
Myocardium
Nephric Tubules
Pharyngeal Arches and Clefts
Proepicardium
Sinus Venosus
Splanchnic Mesoderm
Tail

Entry 5

GEISHA Id	POD1.Braitsch.2012	Data Source	Publication
Complete cDNA Template Probe	show TTTGGCATCTTCCAGACCATGTCCACTGGGTCCCTCAGTGATGTGGAAGATCTGCAAGAGGTGGAAATGCTGGAGTGCG ATGGCCTGAAAATGGATACTAACAAAGAGTTCGGGGCGTCCAACGAGAGCAACGAGGAGGGATCCAATGGCGAGAATGG CTCCCCTCAGAAAGGGAGAGGGGCCTCGGGAAAGAGGAAAAAGGCTCCCCCCAAGAAGAGCCCTTTAAATGGTGTGAGC CAGGAAGGAAAGCAGGTCCAGAGAAACGCTGCCAACGCAAGGGAGAGGGCAAGGATGAGGGTCCTTAGCAAAGCCTTCT CCAGGCTTAAGACCACCCTGCCCTGGGTGCCCCCAGACACCAAGCTTTCCAAACTGGACACCTTGAGGCTGGCCTCCAG CTACATCGCACACCTGAGGCAAATCCTGGCCAACGACAAGTACGAAAATGGCTACATCCATCCAGTCAACCTGACCTGG CCTTTTATGGTAGCCGGCAAACCCGAGAGTGACCTGAA
Citation	Braitsch CM, Combs MD, Quaggin SE, Yutzey KE. Pod1/Tcf21 is regulated by retinoic acid signaling and inhibits differentiation of epicardium-derived cells into smooth muscle in the developing heart. Dev Biol. 2012 Aug 15;368(2):345-57.
Copyright	Copyright © 2012 Elsevier Inc.
Comments	The cDNA template used to generate the probe was obtained by PCR as described in the paper.

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 31

Epicardium

EPDCs, Epicardium-derived cells(arrow heads)

Entry 6

GEISHA Id	TCF21.Anderson.2020	Data Source	Direct Submission
Complete cDNA Template Probe	show AGAGAAGGGGAGGAAAGTGCTGCGCCCCTTCTCTCCTCCACCGCCACCACGTAGGTTTGGGGAGCTCCCCGTCCTCCCC TCCTCCCCCTTTCCCGCCAGCCCTTTGGCATCTTCCAGACCATGTCCACTGGGTCCCTCAGTGATGTGGAAGATCTGCA AGAGGTGGAAATGCTGGAGTGCGATGGCCTGAAAATGGATACTAACAAAGAGTTCGGGGCGTCCAACGAGAGCAACGAG GAGGGATCCAATGGCGAGAATGGCTCCCCTCAGAAAGGGAGAGGGGCCTCGGGAAAGAGGAAAAAGGCTCCCCCCAAGA AGAGCCCTTTAAATGGTGTGAGCCAGGAAGGAAAGCAGGTCCAGAGAAACGCTGCCAACGCAAGGGAGAGGGCAAGGAT GAGGGTCCTTAGCAAAGCCTTCTCCAGGCTTAAGACCACCCTGCCCTGGGTGCCCCCAGACACCAAGCTTTCCAAACTG GACACCTTGAGGCTGGCCTCCAGCTACATCGCACACCTGAGGCAAATCCTGGCCAACGACAAGTACGAAAATGGCTACA TCCATCCAGTCAACCTGACCTGGCCTTTTATGGTAGCCGGCAAACTCTAGAG
Comments	Images and probe sequence provided by Claire Anderson.