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EFNA5
ephrin A5| Gene | | Official Symbol | EFNA5 | | Official Full Name | ephrin A5 | | CGNC ID | 49648 | | also known as | RAGS, ephrin-A5|EPH-related receptor tyrosine kinase ligand 7|LERK-7|repulsive axon guidance signal protein, Ephrin-A5 precursor , LERK-7, RAGS protein, EPH-related receptor tyrosine kinase ligand 7 | | gene type | protein-coding |
| | Genomic Map | | | Sequence Information | | | Gene Expression | | | Orthology | | | Gene Ontology | | Molecular Function | chemorepellent activity, ephrin receptor binding | | Biological Process | brain development, ephrin receptor signaling pathway, negative chemotaxis, more...positive regulation of peptidyl-tyrosine phosphorylation, regulation of actin cytoskeleton organization, regulation of cell-cell adhesion, regulation of focal adhesion assembly, regulation of microtubule cytoskeleton organization, regulation of Rac GTPase activity, regulation of Rho GTPase activity, retinal ganglion cell axon guidance | | Cellular Component | anchored component of external side of plasma membrane, plasma membrane |
| | Links to other databases | |
Entry 1| GEISHA Id | EFNA5.UApcr | Data Source | GEISHA ISH Analysis | | Forward cDNA Template Primer | TTATAAAAGCTTGCGGCCGCAGAATATAACAGCACCAACCCCAGATTC | | Reverse cDNA Template Primer | GCTCTAGAAATTAACCCTCACTAAAGGCAAAAGCATTGCCAGGAGGAAC | cDNA Template Length | 570 | | Forward cDNA Template Probe | show GACTACCACATCGATGTGTGCATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAAGATA
AGACCGAACGCTATGTTCTGTACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCAAGAG
GTGGGAGTGCAATCGGCCACACTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTTCTCA
CTAGGATTTGAGTTCAGGCCAGGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCCTGTC
TAAAGCTTAAGGTCTTTGTGCGACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATGTTAA
CGACAAAGTAGAAAATTCATTAGAACCAGCAGATGATACCGTACATGAGTCAGCCGAGCCATCCCGTGGTGAGAACGCG
GCACAGACACCGAGGATACCCATCCGGCTTTTGGCAACTCTATTGTTCCTCCTGGCAATGCTTTTGCCTTTAGTGAGGG
TTAATTCTAGAGCAA |
Entry 2| GEISHA Id | EphrinA5.Baker.2003 | Data Source | Publication | | Complete cDNA Template Probe | show ATTTTCCGCATCCGTCTCCCCGCGATGCCGCACGTGGAGATGCTGCTGCTGGCGGTCGCGGCGCTGTGGGTGTGCGTGC
GCGGGCAGGAGCCCGGCCGCAAGGCGGTGGCCGACCGCTACGCCGTCTACTGGAACAGCACCAACCCCAGATTCCAGCA
GGGAGACTACCACATCGATGTGTGCATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAA
GATAAGACCGAACGCTATGTTCTGTACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCA
AGAGGTGGGAGTGCAATCGGCCACACTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTT
CTCACTAGGATTTGAGTTCAGGCCAGGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCC
TGTCTAAAGCTTAAGGTCTTTGTGCGACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATG
TTAACGACAAAGTAGAAAATTCATTAGAACCAGCAGATGATACCGTGCGTGAGTCAGCCGAGCCATCCCGTGGTGAGAA
CGCGGCACAGACACCGAGGATACCCATCCGGCTTTTGGCAACTCTATTGTTCCTCCTGGCAATGCTTTTGATATTATAG
CACAGTATGCTCCAGCAACCTGTCGAAGAAAATTTCAGGGTCTTGGAACATCAAAGATCCACCTAACTGCTCATCCCAA
GAAAGGGAC | | Citation | Baker RK, Antin PB. Ephs and ephrins during early stages of chick embryogenesis Dev Dyn. 2003 Sep;228(1):128-42. | | Copyright | 2003 Wiley-Liss, Inc. | | Comments | We are unable to detect ephrinA5 transcription before stage 7. During stages 7-12 (Fig. 1D-F), ephrinA5 expression is prominent in the neural folds, with highest levels present throughout the presumptive brain. Weak ephrinA5 expression is also detected in the head mesenchyme during stages 9-12. |
Entry 3| GEISHA Id | ephrin-A5.Mühleisen.2006 | Data Source | Publication | | Complete cDNA Template Probe | show ATTTTCCGCATCCGTCTCCCCGCGATGCCGCACGTGGAGATGCTGCTGCTGGCGGTCGCGGCGCTGTGGGTGTGCGTGC
GCGGGCAGGAGCCCGGCCGCAAGGCGGTGGCCGACCGCTACGCCGTCTACTGGAACAGCACCAACCCCAGATTCCAGCA
GGGAGACTACCACATCGATGTGTGCATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAA
GATAAGACCGAACGCTATGTTCTGTACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCA
AGAGGTGGGAGTGCAATCGGCCACACTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTT
CTCACTAGGATTTGAGTTCAGGCCAGGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCC
TGTCTAAAGCTTAAGGTCTTTGTGCGACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATG
TTAACGACAAAGTAGAAAATTCATTAGAACCAGCAGATGATACCGTGCGTGAGTCAGCCGAGCCATCCCGTGGTGAGAA
CGCGGCACAGACACCGAGGATACCCATCCGGCTTTTGGCAACTCTATTGTTCCTCCTGGCAATGCTTTTGATATTATAG
CACAGTATGCTCCAGCAACCTGTCGAAGAAAATTTCAGGGTCTTGGAACATCAAAGATCCACCTAACTGCTCATCCCAA
GAAAGGGAC | | Citation | Mühleisen TW, Agoston Z, Schulte D. Retroviral misexpression of cVax disturbs retinal ganglion cell axon fasciculation and intraretinal pathfinding in vivo and guidance of nasal ganglion cell axons in vivo. Dev Biol. 2006 Sep 1;297(1):59-73. | | Copyright | Reprinted from Developmental Biology, Volume 297, Thomas W. Mühleisen, Zsuzsa Agoston and Dorothea Schulte, Retroviral misexpression of cVax disturbs retinal ganglion cell axon fasciculation and intaretinal pathfinding in vivo and guidance of nasal ganglion cell axons in vitro, Pages 56-73, Copyright 2006, with permission from Elsevier.
Copyright © 2006 Elsevier B.V. All rights reserved. | | Comments | According to the authors, "The cDNA clones used to generate in situ probes for EphA3, EphA4, ephrin-A5, ephrin-A2, EphB2, EphB3, ephrin-B1, ephrin-B2 or RCS, which recognizes a viral core protein, were described elsewhere (Schulte et al., 1999) or were gifts from J. Flanagan, C. Cepko (Harvard Medical School, Boston), or E. Pasquale (The Burnham Institute, La Jolla, California; Holash and Pasquale, 1995 and Cheng et al., 1995). As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_205184) |
| Stage | Image (click image to view full size) | Location (click to highlight) | Comments (click to highlight) | | 19-22 |  | | stage 22 |
| Eye
Retina
|
Entry 4| GEISHA Id | Ephrin-A5.Marin.2001 | Data Source | Publication | | Complete cDNA Template Probe | show ATTTTCCGCATCCGTCTCCCCGCGATGCCGCACGTGGAGATGCTGCTGCTGGCGGTCGCGGCGCTGTGGGTGTGCGTGC
GCGGGCAGGAGCCCGGCCGCAAGGCGGTGGCCGACCGCTACGCCGTCTACTGGAACAGCACCAACCCCAGATTCCAGCA
GGGAGACTACCACATCGATGTGTGCATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAA
GATAAGACCGAACGCTATGTTCTGTACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCA
AGAGGTGGGAGTGCAATCGGCCACACTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTT
CTCACTAGGATTTGAGTTCAGGCCAGGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCC
TGTCTAAAGCTTAAGGTCTTTGTGCGACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATG
TTAACGACAAAGTAGAAAATTCATTAGAACCAGCAGATGATACCGTGCGTGAGTCAGCCGAGCCATCCCGTGGTGAGAA
CGCGGCACAGACACCGAGGATACCCATCCGGCTTTTGGCAACTCTATTGTTCCTCCTGGCAATGCTTTTGATATTATAG
CACAGTATGCTCCAGCAACCTGTCGAAGAAAATTTCAGGGTCTTGGAACATCAAAGATCCACCTAACTGCTCATCCCAA
GAAAGGGAC | | Citation | Marín O, Blanco MJ, Nieto MA (2001). Differential expression of Eph receptors and ephrins correlates with the formation of topographic projections in primary and secondary visual circuits of the embryonic chick forebrain. Dev Biol. 2001 Jun 15;234(2):289-303. | | Copyright | Copyright © 2001 Academic Press. All rights reserved. | | Comments | Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205184). |
| Stage | Image (click image to view full size) | Location (click to highlight) | Comments (click to highlight) | | 31-38 |  | | stage 35 |
 | | stage 35 |
 | | stage 35 |
 | | stage 35 |
 | | stage 38 |
 | | stage 38 |
 | | stage 38 |
 | | stage 38 |
 | | stage 38 |
| Diencephalon
Midbrain
|
Entry 5| GEISHA Id | EPHRINA5.Takahashi.2003 | Data Source | Publication | | Complete cDNA Template Probe | show ATGCCGCACGTGGAGATGCTGCTGCTGGCGGTCGCGGCGCTGTGGGTGTGCGTGCGCGGGCAGGAGCCCGGCCGCAAGG
CGGTGGCCGACCGCTACGCCGTCTACTGGAACAGCACCAACCCCAGATTCCAGCAGGGAGACTACCACATCGATGTGTG
CATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAAGATAAGACCGAACGCTATGTTCTG
TACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCAAGAGGTGGGAGTGCAATCGGCCAC
ACTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTTCTCACTAGGATTTGAGTTCAGGCC
AGGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCCTGTCTAAAGCTTAAGGTCTTTGTG
CGACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATGTTAACGACAAAGTAGAAAATTCAT
TAGAACCAGCAGATGATACCGTGCGTGAGTCAGCCGAGCCATCCCGTGGTGAGAACGCGGCACAGACACCGAGGATACC
CATCCGGCTTTTGGCAACTCTATTGTTCCTCCTGGCAATGCTTTTGATA | | Citation | Takahashi H, Shintani T, Sakuta H, Noda M. CBF1 controls the retinotectal topographical map along the anteroposterior axis through multiple mechanisms. Development. 2003 Nov;130(21):5203-15. Epub 2003 Sep 3. | | Copyright | Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. | | Comments | The authors indicated the probe was the 681 bp fragment of chick ephrin A5 (25-705; X90377) |
| Stage | Image (click image to view full size) | Location (click to highlight) | Comments (click to highlight) | | 7-12 |  | | stage 12 |
| Retina
| | 13-18 |  | | stage 18 |
 | | stage 18 |
 | | stage 18 |
 | | stage 18 |
| Retina
| | 31-34 |  | | stage 34 |
 | | stage 34 |
 | | stage 34 |
 | | stage 34 |
 | | stage 34 |
| Retina
|
Entry 6| GEISHA Id | EPHA5.Jayasena.2005 | Data Source | Publication | | Complete cDNA Template Probe | show GGTGGCCGACCGCTACGCCGTCTACTGGAACAGCACCAACCCCAGATTCCAGCAGGGAGACTACCACATCGATGTGTGC
ATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAAGATAAGACCGAACGCTATGTTCTGT
ACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCAAGAGGTGGGAGTGCAATCGGCCACA
CTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTTCTCACTAGGATTTGAGTTCAGGCCA
GGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCCTGTCTAAAGCTTAAGGTCTTTGTGC
GACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATGTTAACGACAAAGTAGAAAATTCATT
AGAACCAGCAGATGATACCGTGCGTGAGTCAGCCGAGCCATCCCGTGGTGAGAACGCGGCACAGACACCGAGGAT | | Citation | Jayasena CS, Flood WD, Koblar SA. High EphA3 expressing ophthalmic trigeminal sensory axons are sensitive to ephrin-A5-Fc: implications for lobe specific axon guidance. Neuroscience. 2005;135(1):97-109. | | Copyright | Copyright © 2005 IBRO. Published by Elsevier Ltd. All rights reserved. | | Comments | The complete cDNA template sequence was obtained from the information provided in the publication as described in Jayasena et al., 2005. |
| Stage | Image (click image to view full size) | Location (click to highlight) | Comments (click to highlight) | | 13 |  | | stage 13 |
 | | stage 13 |
 | | stage 13 |
| Eye
Head Mesenchyme
Midbrain
| | all | | |
Entry 7| GEISHA Id | ephrin‐A5.Iwamasa.1999 | Data Source | Publication | | Complete cDNA Template Probe | show TTTTCCGCATCCGTCTCCCCGCGATGCCGCACGTGGAGATGCTGCTGCTGGCGGTCGCGGCGCTGTGGGTGTGCGTGCG
CGGGCAGGAGCCCGGCCGCAAGGCGGTGGCCGACCGCTACGCCGTCTACTGGAACAGCACCAACCCCAGATTCCAGCAG
GGAGACTACCACATCGATGTGTGCATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAAG
ATAAGACCGAACGCTATGTTCTGTACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCAA
GAGGTGGGAGTGCAATCGGCCACACTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTTC
TCACTAGGATTTGAGTTCAGGCCAGGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCCT
GTCTAAAGCTTAAGGTCTTTGTGCGACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATGT
TAACGACAAAGTAGAAAATTCATTAGAACCAGCAGATGATACCGTGCGTGAGTCAGCCGAGCCATCCCGTGGTGAGAAC
GCGGCACAGACACCGAGGATACCCATCCGGCTTTTGGCAACTCTATTGTTCCTCCTGGCAATGCTTTTGATATTATAGC
ACAGTATGCTCCAGCAACCTGTCGAAGAAAATTTCAGGGTCTTGGAACATCAAAGATCCACCTAACTGCTCATCCCAAG
AAAGGGAC | | Citation | Iwamasa H, Ohta K, Yamada T, Ushijima K, Terasaki H, Tanaka H. Expression of Eph receptor tyrosine kinases and their ligands in chick embryonic motor neurons and hindlimb muscles. Dev Growth Differ. 1999 Dec;41(6):685-98. | | Copyright | Copyright © 1999 John Wiley & Sons, Inc. | | Comments | The complete cDNA template sequence was obtained from the information from the publication as described in Iwamasa et al.1999. |
| Stage | Image (click image to view full size) | Location (click to highlight) | Comments (click to highlight) | | 19-24 |  | | stage 23 |
 | | stage 23 |
 | | stage 23 |
 | | stage 23 |
| Spinal Cord
| | 25-30 |  | | stage 27 |
 | | stage 27 |
 | | stage 27 |
 | | stage 27 |
 | | stage 29 |
 | | stage 29 |
 | | stage 29 |
 | | stage 29 |
| Spinal Cord
| | 31-44 |  | | stage 32 |
 | | stage 32 |
 | | stage 32 |
 | | stage 32 |
 | | stage 44 |
| Spinal Cord
|
Entry 8| GEISHA Id | ephrin-A5.Araujo.1998 | Data Source | Publication | | Complete cDNA Template Probe | show TTTTCCGCATCCGTCTCCCCGCGATGCCGCACGTGGAGATGCTGCTGCTGGCGGTCGCGGCGCTGTGGGTGTGCGTGCG
CGGGCAGGAGCCCGGCCGCAAGGCGGTGGCCGACCGCTACGCCGTCTACTGGAACAGCACCAACCCCAGATTCCAGCAG
GGAGACTACCACATCGATGTGTGCATCAACGACTACCTGGATGTATTCTGCCCTCACTATGAAGACTCGGTGCCAGAAG
ATAAGACCGAACGCTATGTTCTGTACATGGTGAACTTTGATGGCTACAGCTCCTGCGATCACATTTCCAAAGGGTTCAA
GAGGTGGGAGTGCAATCGGCCACACTCCCCGAATGGACCGCTGAAGTTCTCAGAAAAATTCCAGCTCTTCACACCCTTC
TCACTAGGATTTGAGTTCAGGCCAGGCCGGGAGTATTTCTACATCTCTTCTGCGATCCCAGATAATGGAAGGAGATCCT
GTCTAAAGCTTAAGGTCTTTGTGCGACCAGCAAACAGCTGTATGAAAACTATAGGTGTTCATGATCGTGTTTTCGATGT
TAACGACAAAGTAGAAAATTCATTAGAACCAGCAGATGATACCGTGCGTGAGTCAGCCGAGCCATCCCGTGGTGAGAAC
GCGGCACAGACACCGAGGATACCCATCCGGCTTTTGGCAACTCTATTGTTCCTCCTGGCAATGCTTTTGATATTATAGC
ACAGTATGCTCCAGCAACCTGTCGAAGAAAATTTCAGGGTCTTGGAACATCAAAGATCCACCTAACTGCTCATCCCAAG
AAAGGGAC | | Citation | Araujo M, Piedra ME, Herrera MT, Ros MA, Nieto MA. The expression and regulation of chick EphA7 suggests roles in limb patterning and innervation. Development. 1998 Nov;125(21):4195-204. | | Copyright | © The Company of Biologists Limited 1998 | | Comments | Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205184.2). |
| Stage | Image (click image to view full size) | Location (click to highlight) | Comments (click to highlight) | | 25-26 |  | | stage 26 |
 | | stage 26 |
 | | stage 26 |
 | | stage 26 |
| Spinal Cord
Unlabeled
|
Entry 9| GEISHA Id | EFNA5.Anderson.2020 | Data Source | Direct Submission | | Complete cDNA Template Probe | show GTAAAACTTAAAGCTATGTTCTTTAAATTCTGTATTATTAGCAACCTCTGTTGTATATAGTGTTTGATAATAAAGTATT
AATTTGATTCTTATGTCTTTTGTAAGTGAGAACAATAGACTTTCAGGATATTAAAATCATGCATTGATTACAAGACAGA
GCTTTGAAGCATCAAGTGTGATCATGGCTGAGAACTGAGAGACAAGTGGCCATGACACTGCAGCATACAGGACTCATCT
TTGGAAATCGTTCATCTATTGCAAGTCTTCACATTGTCAAGACCTGGAAAACATGACTTTTGGTGATCAGTGTTTTGTC
CTCAGTGTTTAAAGGTCTAATGTTGGAGCCCCATGGTTTCTGTGTTAGGTTTTGTTCTTCTGTATCATGTTTGTTTCTA
AAATTGCTTTGGGGATATAACAACTCTTCTGTTTATTTGGTTTTTGTTGTTGTTTAGGTTTTTGGGTTTTTTTTTTCCT
TTATTCCTTCTGATTTTCTTTCCATGAATAGGTTTTTGTTTTGGTTTGGTTTGGTTTTTTTCCGGAAAGGATCTTGCAA
GTGTCATGTGTGATATGTACGTACTTGGTTCCAAAACAACTAGAAACAAATTTTGGGAGGTATTGAGAGTAGATGAGTA
GATTGTTTCCCTTTACTATATAATATGGAAAGGAAATGGTTGTATTTATACCTTGTTGATTCCAACCCTTACCCCTAAA
ACTGAATTTTATCCATTGCCTTGTGAGACAGGTGGCTAATTAACTTTAAAAGAATTTAAAGAGGTGGTTAACTTCTTCC
CCTTCTTTCTTACACCTTGGATTTTGGAATCCACCGG | | Comments | Images and probe sequence provided by Claire Anderson. The probe was synthesized from cDNA clone ChEST290o16 (GenBank: CR406662.1). |
| | Data from NCBI Unigene EST Profile
Unigene ID: Gga.651 |
MouseFrogFruit FlyZebra FinchZebrafish |
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