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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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SOX2
SRY-box 2

The human orthologue of this gene is associated
with the following human diseases:

Gene

Official Symbol	SOX2
Official Full Name	SRY-box 2
CGNC ID	49650
also known as	transcription factor SOX-2\|SRY (sex determining region Y)-box 2\|Sox2 transcription factor\|cSox2\|delta EF2a, Transcription factor SOX-2
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_205188
Polypeptide	NP_990519
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.601

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	6657	ENSG00000181449	MIM:206900	MIM:184429
Mouse	20674	ENSMUSG00000074637	All phenotypic alleles (16):Radiation induced(1) Targeted, knock-out(4) Targeted, other(10) Transgenic(1)	MGI:98364
Xenopus	407873, 398000			484552
Zebrafish	378723	ENSDARG00000070913	ZDB-GENE-030909-1	ZFIN:ZDB-GENE-030909-1

Gene Ontology

Molecular Function	miRNA binding, sequence-specific DNA binding, more...
Biological Process	cell cycle arrest, endodermal cell fate specification, eye development, inner ear development, more...
Cellular Component	cytosol, nucleus

Links to other databases

Entrez Gene	396105
Ensembl Gene
KEGG	gga:396105

Entry 1

GEISHA Id	Sox2.Matsushita.2002	Data Source	Publication
Complete cDNA Template Probe	show TGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGGGAGGCACAGGCAACTCCAACTC GGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTTCATGGTGTGGTCCCGCGGGCAG CGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGGCTCGGGGCCGAGTGGAAACTTT TATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGCACATGAAGGAGCACCCGGATTA TAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTTGCCAGGGGGCTTACTGGCGCCG GGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTGAACCAGAGGATGGACAGTTACG CGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCTACCCGCAGCACCCGGGCTTGAA CGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCAGTACAACTCCATGACCAGCTCG CAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACCCCGGGCATGGCGCTGGGCTCCA TGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGTCTCATTCGAGGGCTCCTTGCCA AGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGAACCAGCTGCCCCAAGCAGACTT CATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACACTCCCTCTATCACATATGTGAA GAC
Citation	Matsushita S, Ishii Y, Scotting PJ, Kuroiwa A, Yasugi S. Pre-gut endoderm of chick embryos is regionalized by 1.5 days of development. Dev Dyn. 2002 Jan;223(1):33-47.
Copyright	Copyright © 2002 Wiley-Liss., Inc.

Stage

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Location (click to highlight)

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7-10

stage 10

Endoderm, early
Gut
Neural Plate/Tube

pre-gut endoderm

Entry 2

GEISHA Id	Sox2.Rex.1997	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Rex M, Orme A, Uwanogho D, Tointon K, Wigmore PM, Sharpe PT, Scotting PJ. Dynamic expression of chicken Sox2 and Sox3 genes in ectoderm induced to form neural tissue. Dev Dyn. 1997 Jul;209(3):323-32.
Copyright	Wiley-Liss., Inc. 1997
Comments	Insufficient information in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # U12532).

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Area Pellucida
Epiblast
Hensen's Node
Neural Plate/Tube

7-8

stage 8

Neural Plate/Tube

Entry 3

GEISHA Id	Sox2.Chapman.2008	Data Source	Direct Submission
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Comments	Images Provided by Susan Chapman.

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Neural Plate/Tube

Entry 4

GEISHA Id	Sox2.Matsumata.2005	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Matsumata M, Uchikawa M, Kamachi Y, Kondoh H. Multiple N-cadherin enhancers identified by systematic functional screening indicate its Group B1 SOX-dependent regulation in neural and placodal development. Dev Biol. 2005 Oct 15;286(2):601-17. Epub 2005 Sep 16.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205188).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

stage 11

stage 12

Central Nervous System
Ear/Otic Placcode
Ectoderm
Lens
Sensory Organs

Lens placode

13-14

stage 13

stage 14

Ectoderm
Lens
Nasal Placcode/Nerve

Lens placode

Entry 5

GEISHA Id	SOX2.Streit.2002	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Streit, A. Extensive cell movements accompany formation of the otic placode. 2002 DEVELOPMENTAL BIOLOGY 249(2), 237-54.
Copyright	"Reprinted from Developmental Biology, 249(2), Streit A., Extensive cell movements accompany formation of the otic placode, 237-54, Copyright (2002), with permission from Elsevier". Copyright © (2002) Elsevier B.V. All rights reserved.
Comments	According to the authors, cDNAs came from various sources (refs). As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence was obtained from NCBI (acc#NM_205188).

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

stage 6

Neural Crest
Neural Plate/Tube

7-12

stage 7

stage 8

Headfold
Neural Crest
Neural Plate/Tube

double labeled

31-44

stage 44

Entry 6

GEISHA Id	SOX2.Streit.1997	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Streit A, Sockanathan S, Pérez L, Rex M, Scotting PJ, Sharpe PT, Lovell-Badge R, Stern CD. Preventing the loss of competence for neural induction: HGF/SF, L5 and Sox-2. Development. 1997 Mar;124(6):1191-202.
Copyright	Copyright: © 1997 Streit. et al.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_205188).

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Epiblast
Primitive Streak

4-6

stage 6

Neural Plate/Tube

7-9

stage 8

stage 9

Central Nervous System
Neural Plate/Tube

Entry 7

GEISHA Id	SOX2.Linker.2004.	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Linker C, Stern CD (2004). Neural induction requires BMP inhibition only as a late step, and involves signals other than FGF and Wnt antagonists. Development. 2004 Nov;131(22):5671-81.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_205188)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

Neural Plate/Tube

7-9

stage 9

Neural Plate/Tube

Entry 8

GEISHA Id	Sox2.Hyre.2003	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Hyer J, Kuhlman J, Afif E, Mikawa T. Optic cup morphogenesis requires pre-lens ectoderm but not lens differentiation. Dev Biol. 2003 Jul 15;259(2):351-63.
Copyright	Copyright © 2003 Elsevier Science (USA) All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205188).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 13

Eye
Lens

Neuroepithelium

Entry 9

GEISHA Id	Sox2.Basch.2006	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Basch ML, Bronner-Fraser M, García-Castro MI. Specification of the neural crest occurs during gastrulation and requires Pax7. Nature. 2006 May 11;441(7090):218-22.
Copyright	Copyright © 2006, Nature Publishing Group
Comments	Insufficient information provided in publication to verify exact sequence to synthesize probe. Sequence below was obtained from NCBI (NM_205188).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

stage 6

Epiblast
Neural Plate/Tube

double label

Entry 10

GEISHA Id	SOX2.Huang.2010	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Huang C, Chen J, Zhang T, Zhu Q, Xiang Y, Chen CD, Jing N. The dual histone demethylase KDM7A promotes neural induction in early chick embryos. Dev Dyn. 2010 Dec;239(12):3350-7.
Copyright	Dev. Dyn Copyright © 2010 John Wiley & Sons, Inc. All Rights Reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_205188).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

stage 2

stage 3

Neural Plate/Tube
Notochord
Unlabeled

4-6

stage 4

stage 5

stage 6

Epiblast
Neural Plate/Tube
Notochord

stage 7

Neural Plate/Tube
Notochord

Entry 11

GEISHA Id	SOX2.Chapman.2003	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Chapman SC, Schubert FR, Schoenwolf GC, Lumsden A. Anterior identity is established in chick epiblast by hypoblast and anterior definitive endoderm. Development. 2003 Nov;130(21):5091-101. Epub 2003 Aug 27.
Copyright	© 2003 The Company of Biologists Ltd
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205188).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Neural Plate/Tube

St. 3d/4

4-5

stage 4

stage 5

Neural Plate/Tube

Double in situ hybridization - St. 4+/5
Double in situ hybridization - St. 4/4+
Double in situ hybridization - St. 5+
St. 4/4+
St. 5+

Entry 12

GEISHA Id	SOX2.Stuhlmiller.2012	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Stuhlmiller TJ, García-Castro MI. FGF/MAPK signaling is required in the gastrula epiblast for avian neural crest induction. Development. 2012 Jan;139(2):289-300.
Copyright	© 2012. Published by The Company of Biologists Ltd
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205188.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-5

stage 5

Epiblast

Entry 13

GEISHA Id	SOX2.Wakamatsu.2000	Data Source	Publication
Complete cDNA Template Probe	show GATCTTGAGGAGAGGGCAGTGGTTTCTATCTCTAGCTGTGAAAAGGGACACAATGTCCAGAAAAATGGCTTCAAGGCGT TTCTTTGGAGAGTCTCTTCCCAAGTGTAACCTTTTCCATTGGCTATTGGGAGACCTCTGACCATGCTTCTATTTCTGAA AGTCCTGCAGCTTGTTGCCCCACACCAGCTGCTCAGGTCAAGCCTTCCTGCACCACGGCAAGGTGATCTCCTTCTTACT GCAGCTGTTGAGAAGGATGACCAACTGACAGTAAAATTGCTTGGTACTGATGAGCCTTTGGCTTTGTTACTGTGGTAAA ACATACATCATTTGACAGCTGCTTGTGCATGGAGAAAACCATAGATGATTAACGGTTTCCTGTCTTTTCTTGCTACTGA TCTTCTTTGCTTATGACAGATGAGACTCAGATGTCTTTTTTTAAGAGGCAGAGAGAGAAAGGGAGGATATTTAAACTTG TTCTCACCTAAGTCTTTTATGTTATCCTAAGGACCTTATTTGTAGTAGAGATTTTTTTTTCTGCATTTTAGCTCTCCTA AAATGATCAGATCTGTCATTCAGCTTTAAAAGGGAATGTATATTCTTCCTCTCCTGGATTTTTTTTCCCCCAAGGGGTA TAATGCTAATTTTACTTATTCAGAAATTAAAATAGCAAACAGTTTATGTGAGGTGTTGATAATTACCTATATTACTGTA ACGCTGTGACAGATGTTGGCAAAAAGAAGCAGAAAATGCCTACAGCTGTGCACTCCATGACATCCAGTTTATACTCAGA CAGAAGGCTAACAGATGTTCTGAACTGTCACTTTGCCTAAATGTTTTTAAAGTCTAAGGACCTGCTTTATAATTCACTG GGGCCTCATTTGGTTTCGCAGAGGTCCTCAGAATTCCCATCTGTGACAATTAATGTTGAAGACTTTTATAAATCAACCC TTGTCCTTATTAGCCCTCTGCAAATAGATATTGTGTATCTGTATACTCCCGAGACTTCTATTTTTTGTGACCTCGTTAT TAATTGCTACTACTTGTGTATAACATAATTCATGTTTCAAGCAGAAAGAGAAATGGATTACATGAATGAGCATCAAAGA TCACCCAGGTACATTTGACTAACCTCAGAGTACCTATGTTAAAACATTCTCTATTGTATCCCCAATTCTGTGGTCATTT CTAAAGTGATTTGAAAATGCTGACATCTCCAACCTACCTACTCAGTTTCCCAAAGGAAAATACGAATTTTGGTGGTACA AAAAGGTAGCACTTAGGCAGATATGTTCAGCTGCTGGAGCTATTTCTATTCCTGGAGAGTGTTCTAATTCAAAAATAGT GAAATCACTAAGGGAGACTTTAAAATAATTCAAGCTTCCTGTATTATTTCAAAATGGAGTTGTTATGAGGATGGCATGT AAGTAATGTGTGCAGGTAGGAAATCTGAGATTTGAAATAGTCTTTGGGCTCTAAGCTCCACCCAGATTTTCTAGCATAC CTTTAGATAAGCAAAAAATGTCTGTAGCATAAAACAACTTAATACTTTATTGATAGTTGCCATTTCTTTTTAAAAGCTG GGTTCCTGTTGTACAGCAGTTTAATAGCTTTAACATTGCACTGCCGTAATCGGTGTTTGGCCTCTTCTGTTCCCCAATC CTGTATGGAAGCAGAACTCCAAATTGTACATCTTTCTGGGTTTGTTTCTAAATTACTCCCCACACTTAATTACGAGGAT GAGAGGGATGCCTTATTCCTCTTTGTTATAGGCTAAGCTCTCAGTAATCCCAAACAAAAGAGCCAGTTCTGGAAGATAT AGGATTTGCTAGCAGGATTATGGTCTCATAGATAATGATGTGCAGTCAGCAGAAGCGAAGCTTTATTTTAATTCAAATA AGCTAGTAAACAAACCACTGACAAGTCTGCTGGTGAGAGTAACTGGCTTCTGTTGCCACATTGGCAGCTAAATTATAGA AAATCTATTATACCCTGTTCACCATATGTTTAATTTAATACAGATACAAATTAAACTATCCACAGTGCCTATTAAAATA ACAATACATATTTTTAAAATATATGAATGTTTTAAGATACATTCCCCTGACCTTAAGAAGGGCAAATGCTGATTTGGGA GACTACAGCAAAACACATGCTGTAGTCAACAGATTCTCTTCAACAACAACAACAGTAATTTGAAAACCCCTGAATATTA ATAGTACAGTTTTGTAAAAGATAATGAGGTTAATTGGGGTGTTTTACCTTCTAAGCATCTGCTCTTCCGTGTTTCCCTC CCCCTAGGTTCCTGGGCATGGATTTGATAGAAGTGGCTGATATCTAATCCGTTGTACACTTTTTAATTCTGTGCCAGAT GCAGTGTGAGTTGTTTGCCCTTTTCCTGCTGCAAATAGATATCCCTCATTTCTGCATATCCTCATTTGTTAACGTTCTC CTACACAGCCCTCAGCTGGATTTTTGACCTGTTGGGAGCTGCTACTCTGTATCAGTGGCAGAGGGTTTCAAATTGTGTG GAGGGGTTCGTACTCATTGCCCGCGTTCAGAGCAAAAAGTGTTGAGCACTGTGTATTTTGGCCCTCCTTGCACCCTCAT TGCTGTCTCGAGTCCTGTGACGGCTGCAGTGAGAGTCTCTGAGTCTGGGTGCTTCCACATAAACTGAACATACCTCATA AAGTCACTGAGTCTCCAAGTCACTGCTCATAGCAGGCTACGGAGAATGGGGTTAAAGATCAGCAGCTGTCCCTGTGGTG AGGTGGTGATGATGAGGGACTACCTGAAACCGTACAGAAAGCACGGAGAAAACCTCTCCAAATTCACATTGCAACTGAA GTGAGTGATGACAAAGTGCCAGGCAGCACTGTGCCAGGAAACTGTTCTCTGGATGTTGCTGGAAGCCCTCTGCCAAGGT TGGCTCTGTCTCTTCGTAGTCCCAGGCTGCTGACAGCAGCAATGAGCGTGTAATGGGACAGGAGTTCTGAGAAATGACC TGATGTGGTTTGGGGACTGCCACCTTACTCACAGCAATATGTTCAGAATTCAGCATTCAGAATGATCTCTTATCACTTA GGATGTAAGAACACATGCATACCCCACAGACGTGTGGAAGTGTGTGTGCAGAAAGGGGTCTCAGTCCTCTGAATGCCAG GTATCGTGTGCCATGCATATATAGACTGTGTACTGTCACAGTGTGTAGCATATGTGCAGGTATGTGAAGTACACACATA CTGTATGCATAAATACACACATTGACTATTTAAATTGCTTTAAATTACTGTTGTTATTTGCCCAAGTATTTTCAGAATC AATGTATTTGTGATACGTTGACAGAACGTACACAGGAAGGTATCAGTTATCAGAAGCAAGTCTAACTTCTGTGACAAAT TCATCTTCAGAGTGTGGAAGAAGCCCAACTCCAGAGAAAGTTGCCATCTAAAGGACTGAAAAAAAGGGAGTTGTCGAGT CCTTAAGATGTGTGGGCAAAGTGCTAACAGATTATTTCCCAGGAGAAATGCATCCTTTCCTCTGTGCTCATCTTTCAGT AAAGGCGTATTTGGCACTGCAGCTATCCACAGCAATTGGCAGCAGAAATATTGCAGTGAATGTTGAGATAATGAATGAA CAATTTCGCATCTCTTAGAGCGGTCACGGGGAGGGAAAAAAAAAAAAGCCAACTAAGAAGTCTCCAGGTTTGGGAGGCA CGGCTGAAGCTCACACGTACATTTCTGCACAGCGAAGGGCCTGCCTGCCCTGCAAACCTGCAGCTCCTCGGGAGACTGC GTGTTCTTTGCTTGCAGTTAAGCGGGCTCCTTACTATCGTATCCCAGGACAAAACAAACAACCTGCTGTCACAAATGTT CTTTTAACACCGTGAGGTGGTGGGAAGCTTTTCTAGCTCTTCGAAGGACCGCGTGTCCGTACGACGGGTGTGAATTCTG GTGAGCTGTCTGCACGCCATGGGGATAGAAAAGCCCGAACGTGCTTTAAGTGAAGGCGTGCTGCCGAGAACTTATGGGA ACGATCGCACGGGAGAACTTTCCCTGGTGAAAAGAGGGAAACGCAGTGAAAGGACTCGCGAGTGAGCGGCCCCGCGGCC CGGGGTCCTTTCGGGCACCTCCATCGCCGTCCTCCGGGGCGCGGGGAGCGCAGCGGCTCCGCTCGGGGGCGGCTGCGGG ACGGCCGTGTGCACGCTGTGAGCGTCCCGAGCCGAACCCGCGGCGAGCAGAGCGCGTCCGTGTGTACGCCGGGAGCGCG CAGCCTGCGGCCGGGCAACGGGCAGAGCGCTCCTGGTGCCGAGGTGTTCGTTGGCGCGGGGCCCTGCTTGCCCCGGGCG GGGCGGCCCGACTTCGGGAGTCGTTTGCTCCCCGGGCGCGGCGGTGCGTCGGGCGTCGCTGAGCGCCTCTTACAGAGAG CGCCTCTGGCCAACTGCTCGGGTATCCGGTGTGAGATAAGATAGGGATCTGCTGCGTAAATTTGGAGTTATGCGCTATT TCATCTATACTGCTAGATGCAGGTGTTTTGTTCTGTTCTGTTTCTCGAGTCTTAAATGAATTGTGAAAACCAAAATACC ACTCTGTGGTTCTATGACATTCAATTTCCCATCCCGTGGGAGCGGTTTCGGGACACAGCTTACGCGTATCGGTGCCGCG CGGGGCGAGAAAGGCGTGTTGGGATGTGAGTTTCCCTCAGAGCCCTTCCAGCCCGCGGTTCACGCGTTTTCCTTCTTTT TTTTCTTCTTGGTTATTCCCCCTCCCCGAGGAGGTGTCACACGTTGATCCCGCGCATCTGAAAGTTGCAGCCGCCCGGT CCCACGCGCTGATGATTTCGTTTCCGGCTATCAGCAAAATGAGCTTTAAGCCAATTTCGTGCTTCGGGCTAAAGAGAGA GGGATTCGTGTTTCCCTCCTCGGTGCTTGGCATTCCCGGAATCTCTTTGTCAGCTTGCTGTAGGACGCTGCTGTAATTT GCTTGGAAACTCTCTCCAAGAGCTGTATACAGCTGTTAAGAACGACGTGCAATTTCTGAGCCATCGGGATGCTTTGTCT GAAAAAAAAAAAAAAAATCATCTTTCCCCGCTAAGTCCGTATTAAACTGCTCAGAGCCCGGTTCCCATTTAACCCAAAG CACCTCTATAGGTAGCGAAACGCGCCTTTCCTACTAATCCCTTTCCTTCACTGCTGTGAGTAACAAAATACCTCTTCTA TAGAAGCGGCACTTTCTTCCCTAATGTTGCCCTGTGTTTAGGAAAGCACTTGCACGATTTCCCCAACTTTGGCACGTTT GGGTTCTTGCGCCACTGACATTTTTTTTCCTTCGCGCTTAGAGGTGCAGTGATAGGAGGTCTCTTATGTGCGCTCCGCT GCCCTCTGGGCGTTAAGCCCGGGCAGAGCAGTGCCCCCGTCCGCTGGCAGCGGGGGCCGAGGGGCGGCACGGGGCGGCT GGAGGGGCCCGGGACCGAGCGGAGCGGCCGTGCGGGGAGCGGAGGGAACGCGGCCACCGCCCCGGGGCCGCTCGGAGCC GCCTCGGCGTCGCTCCCCGCTCCCTCCCCGCTCGGCTGGGGCGAGCCGGCGCGAAGATGTTTATCCTGAGATAGAAAAT GGGTTGTTGAAAAACAAGCTATGCGCAAATAGTTTAATTTTCCGTTTTGATGCTTATCTTTGTCTCCCGAATTTCATCG GAGAGGCTTTAATTGGCGTGTGGGTAATAGTTCCTAAATAATCCCTTATTAGATGACTTCAGACGAGTTGGGAACCAAT AACGTCTTAGTCCCAAAAGTGAAAGTAGAATTGGAAGGCGCGGGTCAGTTTTTGTCAAAGGGCTCATTTGGGCATCTTT GCGTCTGCTGAAAAAGCTAATTAAAGAATGTTTGAAGGTTAAAGTCATTATTCCGTGCTGTTGTCGTGCTACAAGTGAG CCGAGTTTTGTGAAAACACAAAGGGCAGAAGTCACAGAGGTGGATTACTGTCAGTCCCGGCTACAGAGGCACAGAGCAC TGTGGCATTAATTCCTATAGAAAGGTAGACAAACACAGGCACTGCCTAACAACTTTCCCGCAGAGCCCCATTGTCTAAG CACTTTCTTTGGCTTCGGGGCTCTCCTCTGCTCGCTACAGTCTCAACCGCGTTTAGAAGCGGGCAACGGGCAGCGCTTC GGGCGCGCTATTCTGGACCCGGGAAAGAATTTCCCAGCTGCGCTTCTCGCCGGTTCCGTGCTGTCCATTTTTCCCTCGG AGCGCTGCTCGTGTATTCATTACTCGGCACAAAGTTGGCGGGGCACGAGAACTCCGCGGCAGTTCGCTGCCCCCACCCG CACGGTGCGCCCCGGGGCTGCAGCTCGGCAGCTGCGGAGGGGTCGGAGAGGCTCCCGGCGTGCAGGGACGAGCGGGGGC CGACGGAACGAGGTCGGCCCCGTTCCCACGCCGGGAGCTGCTCCCGGGGTGCGCGTTCGTGCGCGGGGACGGCAGGTCG CCAAACGACGCGCGCAGAGATGCGCACACGCGCTGTTCGGCCCCGCTTCTCGCGGCTCCTCTGCCTTCGCCTCGGGGCC GCTGTGCCCCTCTCTGCCCCACAGTCCGGGTCGGAGGGGCCGCTGCGGCTGCGGAGCTCCCCACATTCAGAGAAACAGA TGTTTGGGCTGAGCTGAGGGTGGGGGGAGGTGGGGGGAACCCTGCAGCTGCAAGTCAAAGAAATATATTGATAAAATAT TTCAGTAGTAGTAAATACGCGGGGATCGGCTCGACCTTGCGAGGTCTTCATTTTAATTTTTTTTTTCCCCCTATATACA AAAATCTTTATCAAACCTCTATTCTATGGCTTCAATAAACAGAACCCATTACCTCACGGCAGCTTTTGTTGGCCTGTAT ACAAGCGAGAACAAAATGGTTATTCAGGAGAACGCTTTCGATTTTTATTGTGTGTAGTATGCACAAAGAGCAGTGTTAT TGTATTAAAAAGAACCATCTGTAGCAGAAACAATGGCATCAAATTATCTTTGAATACCCACTGCAAAAATATAAAGCTT TTTTATTATTCTTCTGCTGTACGGTGGGGGACAGAGAAGTGACTTTTTTGTTGTCGCGGGTGCATTCTTATAACACACA CAAAATCAGCCATTTGGATTTTTTAAGCCATAAACGATTTTTTCCCCTCTTGTTCCAGGGACCGCAGTTAAAAGCTAGG GCTAGGACTTGGAGGACGTGTAGGCAAACGGAAGGAGAGCCGGGGGAAGAAAAGCCGAAATGGATTCATTCTCCGTCCT GGCTGGAGGAGGAGAGGAGCGGGGACGTGCGGGCTGCCTGCAGCTGCCTCTGTCCGGTGAGAAGTGCTCCCAGCCCCGC GGCTGACTCGGGCTCGGGAGTCCGCGCGGACTTTCGCTCGAAGCTGCCAATCAAACCGCTAACCGCCACCACCCTGCGC TGGCACCGACATGTGAGTACTGCACCACAACTTTGACATTCACGGGTTTCCCCCTTCCTCCCCCGTGACCTTTAAAACG ACGGAGAAATACCGCTGTCGCTTAGCGCCCGCCGCTCCGAGCTGGACGGCGTTACACTGAGGGACTCGCGCGCGGGTTT GGGTTTGCCTGCAACTGCGCGGCAGCCGCTTTGCGACCGGAGCGCCGAGCAGGAGCGGAGCCGCCCGAGGCCGCCCCGC GCTGCCGGCCCCGCGCTGCCCGCCCCTACGCCGGCGCGCACGGACCGAGGGGTCCCCGCGGGCCGCCTCGGCCGGGGCC GCAATTTCGGCGGTGCCAGGTTCCGCTGCGGGGCGGCGGTCGTTTGAGTCACGCCGGCGCTGCGCTGCGCTGTCCGGGG AACGGCGGAGGCGCTCCGCGGGGGCTGGGGGCGGCCGCGCTGCGGGGGTTACCTGGGCGCGCAACGGGCGCGCAGCAGC GCTGGCGCGGCGCCCGCGGCGCCTGCGCTTTGGCGCCCCCTACCGGCGGCCCGCGGCCCGCACCCTCCCGCCGCTCCCC TTCTGCGCGGCGGCTCCGGGGGCGCTCCCGAGGAAAGGAGGGACGCTGGGGGGTCTGCGGCCGCGCGGGGATCCGCGCA GAGCCGTGCGCTGGGAAACGGGGTACACGTACACGAGAGGAAACGCGGGGCGTCCCTGGGCAGAGCCCTCGCTGGGCGA AGAGGCCGTGCCCGGGGAAGTTGCGAGTCAATCGATGCGTGTCACTGCCTGCGCCTAACGCTGCCTCCCGGTGCACTGC AATTTGGGCTGGAAATGATCGATTTTGACATTTTGGTTCATTATAATGCCACTTAAAGGGAGGCTCTTTTTAGTTACAG TTAGCAAAGTTATGGTTGGGTTTTCCCCCTCTTCCCTCCTCCGTCCTCCCCCCCTCCCCCCCCCCCCCCCTCTGTTTTT TGGGGTAAGAATTTGGTAGATAAAGGTTAATCTTGGGCTGAATTATAGAGCTGTGATTGACAAGAGAAAAGGTGTTGAG GGCAGGTCGAGCTGTCTTATTTACAGCCCACTGCTTGCTATAGAGCCTTTGTTAGGACTGGAAAGCTAATTTAGTGCTG ATCAAAAGCACCACGGAGTTAAAGGACTGGTAATGACTGCTAATTTCATTTCAGCCTCATACATCCCCAACCGCTGTAA TTAAAGACGTGCTATTTAAATACAGGTGAAGAGTTTTTGTAGCAGGGGGAAAGTGGGCAGCCGGGTACCATTTTATTTC TGCTTAATGTGGTCAGAGAGCACCGTGTGCCGCGGGGCGAGCGCTGCTTTGTGCGGCCCCGAGGGGACAGCCCCGACCC GCCGGGTTATGTGGGTTTAATTCCTCCCATGCAGCGGCATCGGGAGCGCTGGGAGAGACGAGACGGAGGGTACGTGTCA GCCGTTAAGTGCCTCAGCGGAAGCTCGGGTCCTCGTATTATGATTATTTTTAATTAAATTATTTTCCCACTCCACGGCC GGCTCCGAAACGATGTAATTTCACAACTGATGTAACAGTTTCTTTCTGAGACACATCAAAGGATAAAAGCTCTGCGTTT TCGTGGTTGGTTAAAATTGCTACCAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAGAGAGAGAGGGAATTTAGCCTTTAA TCTCTTTTATCTGCCGAGCCTGAAGATCCGCACTCGACCCCGCTCCTCTCCGGGCTCTCCCCCTCTTCCGGGGCCGCGC CGGGCGGGAGGCGGCGCTGAGCGCGGGTTGCGGCGCCCCCTGTCGGCCGCGCCCGGAGCCGCCCGCGGGGACCTGGCGG AGGAACCGCGGCTCGGCGGCGAGCGGGGCGCGGAGCGGAGCGGTGCTGGGTGTGAGCGTGGGGAGGTGGGGGGTGAGAG AGAGCCCGGAAATCACGGGAAGGGAAAGCGCCTCCAGATCGAGCTCTGTGGCTGCACAGACATTAAACATAATGGCACC GATGTATGTGTGAGCAGAGCTGCCTAAAGCCCCGTCCTTGGCGAAGCGTCCGCGTATGCCGCTGTCGCCCGGCCAATGT GCCGCTGCGGGACCGCGCCGCGGTAGTGGGTGGCAGTGGGCAAATCCCTCGTCCCCCACTGCCGCTGCTGTCGGGACAC TGCAGCCTTCCGGATGCTGTGGTTGTGATTTCCCCCCGAGGCGCTGGAGCTAAGTCGCACTGACAGGTGTTACTTGCGA GCAGAGCTCGGCGGTTCCGTTTCATGTTTCATTAAGGCAAAATGTTCGCAAATGTCATATTTTAAGGCGAAGCGATGAA GTCGTCTTCCTCCTCCCTTCCAAAAAAAAAAAAAAAAAAGAGAGCAAAATAACCCCCCCTCCCCCGATTCCTGGAAAAG GAGAAGCGTTATCTTCAGTGAATGCGTGGAGTGGGGGGTTTCTTCCTCTCTGTTGACAGATTTAAAGGACAGCTGCGTG TTACAAAACCATCAGCCAAAATATGCGAAGTGCCGCATTGTCACCAACTTTTACATGAATTCACCGCTTATTTTATAGG CATCTGCACGGTGTTTGCGAGGTGCGGTGTTAACTGACCGCGATTTGTGCGTCCTGCGTGCGGGGCGGAACGCTAATGG GAACCAAATGATTTCTGCTAAAGCGGGGTGATTTTGTATCTCCTGGTAGCAGCCTGTTTGAAAGCCGTAATGCCGCATT TCTTCTATGAAATGATTCCCATGGCCGTGCGTCTCACAGTAAACTTTTCGCTTTCGACATTAAGTACGAATTGGTGTTT TTAAGGATCATTGTTTGTCGCGCAGAGTATGCGCGCTCCGTTCTCTCGGTTTGATCTCCGCTTGAGACTCCCAATGTGT CCCAAAATAAAGACATCGCATTTTCTATAAATAATAGCAAGAGCCCTGCGCAGATTTCTGCACAACGGAATTCCCAGAT TTCCTTTACTGTTTCTTTCCTCGGTGCACGTTGGGTATATATAACTCTGAAATTAAGCAAAAATATACATCTGTATGAA TCTGTCAAGCAGGCTATAAACAGAGATATTATATGTCAATTACCACAGGAAACGGAAAAAATACCGAGCGGTTGGTACA CACGTTGTAACGTCAGGAAGCGCTTTGCAAAAAACGCAGTGACTCCTGCAGCAGTGTGAATGATTAACCCGGACCCGGA GCGGGGCTGCTGGGGCTCCCCTGGAAACAGCAGTGCTGAGGGGGCTGTAAAGGGCTCGTAAAGTCTCCCCTCCGAGGGG CAGAAGTTGGCGGATAGAGTGAGGCTGTAACCACAACAGGAGCGCTTCCCGGGCTCGCTCGGTGCCAGCTGCGGGTCGA TCCTAAAGCCGGGAGGGAGCCGGGGGTGGCACGCACCTGGCAGCGGGGGGTGGGGGGCCGCTCCGTGCTTGTCCTCTTT CGTCCCCACTTGGCGGTAAAGTGCGGTTTTACTTTACGTATGTCTTTTGGAAGTAGGCTTTATGGATGTCCACATTTCC TCCAGCTCTCGAGGAGCAGAAGAGTGAAGGAAACAAACCCTAAAAGCAACCGGGATGCGGGGCAGATTTGCTCACTCTA CCTTTCCTTTCTCCTCCCCATCCCCAGGCACGGCTGCTGCAAGCCCGTCTTGGGCAGAGGGGTGCTGGGAGCAGCCCCA TTCCCCTGTGACATCACACCGCAACCCAACCCGGGGGTTCAGATTTTTCATGTGCCCTTAAGGTTCTCCTTGTCCCCAC ACACAGGCCTAAGCCCTACGAGGGATGCCGGGGAGCAGCCGGGCTCCCTCCTGAAAAGGCTCTCTCGGGTCATCCTGGG GCAGAGGACAGCAGATAGACACATGGGAAGCGCTGATGGTATTATTTATAGCACCATCTCTCTGCTCCGATTAAGCGCC GTCTAAATATGAATTCACAATATAGACAGCTAGTCTATAATTTGAATTAAAACCCCCCCAACGAGCCCGTCACTTGAGA ATTCTGAAATCACAAGCCTAAGTTGTCACAATGAGCCCATGGTGTCAATAAACCATGGACGAGATGGAGAGATTGTCAC TCCAGACCATAACAGTTCTGAAAGTGTCATTACCGTCTGTCCTGTCAATGAGCTCCAAATTAAGGACTATGAGGAACAG CGAGATAAAAGTCACCGTCTTGGCTCCATTATGGGCGATCGTGTCAGTGATCTGAGAGTCACTGGAAAGACATTGCTTT TTTTCTTTCTTTCTTTTTCTTTATTATTTTTTTTAATGTACTTTTCAAACCGTGCCTCGGGATTCCAGGGTTTGAGGTG CAACCTTGAAGCACCCCGGGCTGTGCCTCAGGCGTTCCCATTGAATCCGCGAGAGCTTCTTTGGGAAACGTCGCGAATT CAATGCGGGAGTCGGAATAATTCCATCAAAACTGCTCCGGGGCCAAAGAGGACTTCATTTGATTTGGGCGACACGTGTG ATCCCATGCACAGACGCGTTTCTACACCTTTTTTTTCTCTTCAGTCAATAGAAAGATGGTGAGGGGGAACGAGGGAAAT AAAATAGAAACCTCAGCTTTGGCAAAAGACAATGGATTTCTGTGCGAGATGGCCCCTTCCAGGCATGAAAACTTTCAGA AAAAGGTCCCAATCTCTCCTTCCTGCGCGCCTGAGAGAGCTGAGGGGACTTATTCAGAAACTGGAAACCTTCAGCTCTG GATTTTTGTGCCTTTGTGGGTTTAGGCAAAGCAGTTAGCGTTCTTCAGGAGGGGTTTTGTGTTTAATATACATGAGTTC ATTCAACTACAATGGGAAAAGAAAACTCATTTGTTCTTATAAAACTAGGAAGGAAAGATAACTTGAAATAGAGTGCCCA GAAGGGCCGCGCTCAGGGGGTGCCGTGCCGGCCTGCGCCCGGCGGCCGTCTCACCCCATGGCACGCAGCAATTCCCGAC CTCATCCTCACCGCTGCTGCTTGGTTTTCTGTTTTATTTTTAAGGTCACGTAAAATAATAATAATAACGGCTTCCATTC TGCTCTGCTCCGGGATGGAGGGCTGGGGAGGGGTGTTCGAGATGCGGGGAAGAGTCACCCAGAGTCGGAGGGCTGTTTC TGGAGCCCTGGGAGCAGAGCAGGTTTGGACACACGGCCTCTCCGCTCGCTGGAAGCCTTCTGAAAGCGGCTTGGAGCGC TGGTGTGTGTGCGTGAGCCGGCTTTGCTGATGTTTATTGTGTACCTTCAACCCGTCCCATCATCTCAGCTTTATGAGTA TATTTTTAAATTTCTCTTATGGCCCCACAGAAGAAAGGTGGGGCCGCTCCCTCTTATTTCCTTTTCGATTTTTTTTTTC TTTAACGCTGTGTTGATGCCTTTTTTTTTTCCTTAACGCCGCTCAAAGTAGCAGCACAGCTGCTGGAGGCGGTGGTCGC GGGGCGGGCGGTGCCCGAGGGGCGGCTCGGAGCGGACAGCCGGCAGGCACAGCCGGGCCCGGAGCGCGGCGCGGGGCGG TTGCGGGGCCACGGGCCGCCCGCACGTGTGCTCGCCCGGCGGTTCCATTGGGAAGAAGGGGGATGAAAGGGGACGCCGG GAGCAACGCGGAGCTCCCTCCGCATTCCCCGGCTTTCAAGGGTCCTTAGAGCCCTTGAATGAAATTATTGGGCGAAATT GGGGCTGGACGCAGGTTTGCGCCTCACCCCCCTCGATCGTTTGTACGTGTAATGCCACTTCTGAGAGCGACAATCGGGC TACTTGCTGGACAGGCAGGGACAAAAAGCAGATTAAACCGTTTTAAATAATAATAAAATATCCCAAACGCAAGCAGAGT GGATTACACTTGCTCCAGCGCCTCGCTCCCCACCGCGTTCCCCCAGCGGCTGCCCTCGCCCTCCGGGCCGCGCCGAGAG GGTGGCCCCGTGCCGGCGGGGTGCGGGGCCCGACGGGGTGCGGGCCGGCTCTGAGCACGGCCCCAACTTCTAAAGTTTG GTGGAGCTTTTTTTCTTCTTCTTCTTTTTTGTCCCCCCTTTTAAGTGCCCCCAGGTTGTCGCTGCGTCTGTCCGTCACC GTCGTGGAATTGTTTGCTGGAGAAAATGTGGCGTTTTCGTCATTTGGGGAGCGTGCAGGAGGCTGAAGGCCCCCCCTGC CCTGCCCTTCCATGGGCCCGGACCCACTCGAAATGGTTTCAGTGAATTGCGGGGACCACTCGGGGTGTTTCTCCCCACC CTCGATAGCTGAAGTCCCCCGGGATTGGGGCGTGGGGTCGTGTGAGGAACTTCCCGGGGAACTTCGGCTCTGCGGAGGG AGAGGGGGGAGAGGAAATTTCTCTGTACGTTTTCGTCTTCGGCTTTTTTTTTTTTAACGTAATGAAATTAAAACTTTTG GAGCTCAGAGTTGACATTTTGCGGAAAATTGAGTTATCAAGGCAGTAATTATTTCACAGGGAGATAAAACTCTCATAGC CCTAACTGTCAAATAGGGCCCTTTTCAGATTTTAATTACAAAATAAAATTAGTCTGCTCTTCCTCGGAAGGGTTTGTGA GTGGCTAAACAGAGCTTTCCCCAATACTGGTGGTCGTCAAACTCTGCTAATTAGCAATGCTGAGAAATTCCAGTTAACA AGGACATTCTCTAAGTCTCTGCAGGTTCCCTGCCGTTCGCCTTCATTTCCATAAGAAGATTAAGAGTGGAGGGGAACAC ACTCAAATGCAGATGCAGAAAAGAAGCGTTTTTAACAAGCATCATAATAGTAAGATGCTTGGCTAGTTCTCACCTAATT ACTGCAAGTTAAACCTCTATTTGCAGCTAAGGACAAAAAATGGAGCTGCAATCTTCCATCTCCACAAGACCGCTTAATA GTTTGCTTAGATTGGTTACTGGCAGGTTCGGAAAAATAATATGAAAATGCGTATATTCTTTCCGATACAGTTAACCCGA TAGGCTGTATCCTCGGGGCTCAGAGAGAATCGGCGATTATTAGCACAATAAATACGCAAAATCCAAGTCGTTAACGTTC ATTTTCTTTTAAAGAAACGCATAACCTGGAAATAAGAGTGCGTGTGCCGCAGAGCCCATCGCCGAGGACGTTTGCGGAC TATTTGCTTAATGCGACCGCTTTGCATTTCCATAAATTACACCGAAGGGAGCGGGTTTATAAATCCGCTCGGACCGAAG CATCCCCGCGCGGCTCCCCCAGCGCCGTGCGGCCGTGCGCGGAGCCGCGGGGTCCGGCCGTGTTCCTCCGCGCACCCAC CGCCCCCAGCGCAGGAGGCGGCTCCTCGGGGAGCCGGACGGCCCTCACCTCGTTTTCTAGAGCGCGTTACGATGTAGAT ACGCCAGATACAGTTGATTGCGTCGATAATTAAAGCAGTTAGTTCAAACGAAGCTGTAAATACCGCCCGATAACGCACT CGCTGGGCAATTCCCACCCTTTATGGGTGCGATAAACGCGGTGAGGAAGGGGCCCGGCCCCGCGCAGAGCCGCGCTCCG GTATCCGTTTGTTACAGCACCGCCGCCCACTCCTCCCCGCTTTTCAAACTTAATTATCCCCGAGATTGCATTATTATCA TTATGATTTGGAATGTTGTTATCAACCGCGGGCCCCGGGCACGACCAATAAGAGAGCTTTCAGCGGCCGTGGGAGCGCG GGGGCAGTCGGGTCGCGCAGAAATCGCGCATCACCGCGGTGTGCGCATCCCGCTACGCGCGCCCGTTTTCCGACGCTTT CAACCCGTCACATCTCTCCAGGTTATATATACACCTTAGAATTACACGCGTTAAAGCCGATAGCGTTTATTTGCATAAT CCAGAGATGCACCCTCGCCCGCCTCACTTTTTCTGCGCGTTACCCGGACGGGGGGGGGCACGGCCGCTCCCTTCCCCCC GCCCCGGCTCCCGCCGCTCCGCACCGTGCCGGCAGGGGGCGCCGCAGTGCCGCGGCTGAGCCCTCGCGGGGCGCCCGGG CGCTGCCCCGGCCCCAACCCGCCCCTGCGCGGGGCCGCGCTTCTCGGGGGGCCCTTCTCGGGGGGCCTCGGCGCTGCCG GGGAGCGCGGCCGCGGGCAAAACAGCGCCGTCGGGGCGCGGCAGCGCGTAGAGCGGGGCCGGGAAGCGCCCCCCCCCCC CGCCCCCCTTTCTCTCCCCTCCTTCCTTTGTCGTCAAGTGCGTTTTAGCAACAAAGATCCCAACAAGAGAGTGGAAGTA AACTTAGCCGGGGCTTTGTGCGGCCCCGTGTAGCGACAACAAGAGAAACAAAACTACCTATTTGTTCCTGCGGGCCGCC CCCCCGCCCGGCGCGCCCACCCAGCGTCGCACAGGGGGCGCGTCCCGGGGCTGCAGGCCGCGCACCGCGTTCTGCGGGG AGGGCCGTCGGGGGACCTCCGCTCCCGCTTCCAGCGGGCACCTCCGGGTTGGAAGGAAGGAGAGAGAAGGGCACGGCCC GGCCCCGTTCTGCGCGGGGCCCGCTCGGCTCCTTTCGGCGGGGCGGCCCCGGGTCAGGGGCTCCGCTCCCCGCGGACGG GGCGAGCGCTGCGCACGCAGCCGGACGGCTCAGGGGACACTTCGCAACGGGGCGCGGGGTCGAGGGGAAGCCGTTCCCG CTCCCCCTATGGCTTCTCCCGACATCAACTTAAAAAACAACAACAACAAAAAACCAACAACGTAGCAACAATGCTGTTT ACCCACTTCCTCCAAAAGGCGTGTGTGACCTGTTGCTGGAAGGGGAGATACAAAGGCTTCCCAGTGGCTGCCTGGGCCG GCCCTGGGCCCGCCCCCCCCGCCGCGCCGTCCCGGCGGCTGAGAGCCCCCGCCCCGTTTCATGCAAAACCAGGGGTAGA GACTTGTCCTCAGTGGCGGAGCCGCCGAGCGCTGATTGGCCGGGGCGAACGCATTTATTCCCTGACAAGCCCCCATCAC ATGGATGGTTGTCTATTAACTTGTTCAAAAAAGTATCAGGAGTTGTCAAGGCAGAGAGAGAGAGTGTGTGTGTTTGCAA AAGGGGGTAAAGTAGTTTGCTGGCTCTTTAAGACCGAGGTGGAGAGAGAGAGAGAGAGGGAGAGAGAGAGGGCGGCAGG CAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATTATT AATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATACCCC TCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCGCTTCTCCGCTCCGGGCTGGT TCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGGG AGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTTC ATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGGC TCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGATGAAGCCAAACGGCTCCGAGCTCTGCA CATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTTG CCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTGA ACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCTA CCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCAG TACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACCC CGGGCATGGCCCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTAGTCACCTCCTCGTC TCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGAA CCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACAC TCCCTCTATCACATATGTAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAAACAAAACAAAA CAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGAGAAAACGGGGCGGGGGGGGGGGAGGGGAGGGGGGCGGGGGGGCAGAAATAAAGCCGACAAAGGAACC TGTATAGATCTCGGAGGGGAAGCGACTACACAAAACTTTTTAAAAGTTCTAGTGAGAACGGTGGGAACTTTGCAGGAAG TTTGCAAAAGTCTTTACCGGTAATATTTAGAGCTAGACTCAAAGAGCGGAGAGGAGAACGTTTTAATATTTGCAGGCAA CTTTTGTACAGTATTTATCAAACTCAACATGGCAATCAGAATGTCCATCGGTCATAGGGAGAGGGGACAAATCAAACCC GAGAATTCGCGAGTGGTTTTTTACACACAGACTCTCCGTGGAGCACCGTGGCCGTGGCAGCTCCGCTGACGGCAGCTGC GGGAGGAGAGGGAATTATTTTGCAGCATTTGGACGTTGAAATTGTTTTAGAAATTGTACAAAAGGCAAAAGCGGTAGGA AGAAGACTGGCGAACCGTGGGCTTGGTTTGCTGGGCGAGGGCAAACGCTTCGATGTACGACGTTTTTATTTTATTTTAT TTTATTTTACTTTATTTTATTTTATTTTATTTTTTACTTCTTGTTAAAAAGCAAAAAAAAATGCCATGAAGGTTCACAC GCCGTTTGTTAATTTATAATAACTTTTTTGTTTCCAGACTTCATCCCATTCAGAGAAAAGGAAAAAAAAAAACAAAAAC AAAAACAAAAAAACCCCAACCTGAAATTACTGTGTTTGAAAGATTTTCCTCTGGTTTGTAATATTTCTGTACATTTATT GTGATGATATTTTAAGTTCTTTTTTTTTTTTTCCTCATTTTCCGTAGTTGTATTTTAAAGATTCGGCTCTGTATTATTT GCAATCAGTCTGCCGACAGTCCATGTATATATTTGAACTAATACCATCCTTATAACAGGTACATATTCAAGTTAAGTTT TTACTCCATTATGCACAGTTTGAGATAAATAAATTTTTGACATATGGACACTGAAGTTCCGTTTGAGTGTTGGATTTAT TTTTGTACCGCAAGCTGGCTTTTTGGTTTTGGTTTTTTTATTTTTCAATAATTCATCAGAGGAAGGTATCCCCGCGGCC GACAGACTGTTATAAATAATGCAGCCGTATACCGAGGCATGAATATTTTTATACGTATGGATAGAGAGGGACCCAGCGC GATACGCTTTACCGGAACGACCGTCCTCGGGGCACGGCGGTGCGGAGCGGAGGCCCCGGTCCCGTCCGGTCCCGTCCCG TGGGCCACTTGTTGCCGGGGCATCCGCGCTCCCTCCGCGCCCGGCGGTGCCGCTCGGAGCCGTGCGGGCCTTCGGGGCG CGGCGCGGGGCTGCAGTGCGGCTGCGGGGCCCGAGCGCTGCCTTTGTTCCGCACTTGCCAAAGTTTCCGCTCCTCGCTC CGGGGCAGCGCAGGAGGAGGTGAGAGGTGGGGGCGGGGGGGGCTCGGCCGGGCAAAGGTAAGGAACGAGAAAAGCGGCG CAAACGAGCAGGACAAAACGAGACGGGCGGACGGCGGAGACTCGCACGCGAAATCACAGCTCTTTCTTGAATCCTATTT CTTAAATTGCCAGCGCGGTGTAGCCCCAATTCGGACAATCTCTGCAGCCGGTCGCAAGCCCCAAAGAGAAGCGCTGGCG CCCGCCAGATAGGAGGAGAGCTCCGCCGTCGGTGCCAGGTAGAGTTCCACTTCGTGCGCTCGGAACCGCCGCCGCCGAG GGCCGCTCGTTGCTGGCCGGGCCCGGCCCGGCCCGGCGGGGGCGGCGGGAGGGGAACGGCGGCGCAGCCCCTCGGCCCC CGGGCGGCCCCGCGTGGGTCCGCGCTGCCAACGCGCCTGCGGCCGCCTCGGGACTGCGCGTGGGGAGGTGCGGATAGGC ACGGGAGGAATACGGCGCCCGAGGAAGTGGGGGGCTGTGATTGCGAAGCGGTCGCGTGTCCTGCTCGTGGCTGTTGTGC TTGGAGGCACGGACGTGGCGGTTTGCTTAGGCACAGGCCGGGTACGAGTGTGGCTCAGCTTTTCTTTCTCGTGTTTCTG GTCGGTCGCCGGATTGCGATTTAGCTCAGGGAGAGACAAAAAAAAAGCGGGCAGAAATGACAAAAGAAGAGTTAGTTGG CTCGCTGTGGTTTGTGTCGTCGTGCTGTTCGCCAGGTTCGGCTCTGGCCTCCGTACTTGGGGCCGGGTGCCCGGAGAGC TCCCGTTGCCGGGCTGTGGGCTGTGGGGTTCTGCTCACTGTGGTGCAGCTCAGCTCCCTTTGTCCCTTTTATTTCCGCG CCGTCGCGGTGTTTGGGCACACGGCAATTACTGCACATTCCCAACCTATTCTGTTTCTTTTGCATTAATGCAACGAGTA AAATATTTTTTTTTCTCAGAGCTGTGCGGCGTTTGTAATGCATCTGGGGATTATTCACGTGGCAGTGAGCGCTCAGCCC CGCTCCGCTCACCTCGCTGAGAAAAGGGGACTGGGGAGAAAAGCCCAATCGATATCTCGATTCCATTTTTTTAGAGCGA ATCTTAGCCAATTAACAGCAATAAAACCGGGCCCCTTTTTGAAGTGCTAACTGAAATAGTCGAGGGGGGTAGCTGGAAT CGTTGGTGCTTTTACGATTCGCATAAAGAAGGCGAGGTAATACCATTAAACAAAGGCACTAAACCACATATTCAGGCTT AGTGTTTTCAGCAACTTTTTATATGGGCAGTTTGTTTGAGCTTAGATTTCGGACTGTGATGTAAAATATATGTTGGTGA AACCTGATCTCCCTTGGGCAGATTCCTCCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCA TCCATTTATCCGTTTACTGAGGATAACCCAAAGCATTTTCTAGGTCCAGCTGAACGCTTTTATTTTTAAAGTGCGACTT GGAAGAACGCCATTGTCCCTGCTAATTTTCAATGCAAAGCATTAAGTAACCCCATTCACACCTCAGCCATTATCCCGGG CGTTTATTGCAGCTGAGATGGGGATAGCGGAGCCCGTGGCTGCGGTTTTATTCTGTTTTCGGAGCCCTTTCGGGTATCC CCTCGGAACTGCTGCGCGTTCCATCCCAGGGCGATGCGCGGTCCATGAGCTTCGGTGTAACATTTCGCCAACGTTGTGC AGAGCCGCAGTGAACCCGAGGATGAGATTAGAGTTTCCCCTGTGATTTTTGTACGGCTGTTTAAGGGCCGGGGTGTTAA AATATTTTGCGGAACTGGAGAGGTAGATTAGCGGGGTTTCTTGCCAAAGCCGGGAAGCTGGTATTACAGCTCGTCCCAG GGGGACATCGCTCACATTAACCACTAAATGAATATTTGACAAGGGCTCATTCGGGGGTATTATTACTACAAACGGGTCC CTACTGGACTTTCCGAGGGTCAAAGAGCAATGGTTCAATTAATTATTTAGACTGTTAGGAGATGCGATTGTAATAGCGG CGCTTATTTCTGCAACCGACGGTTCTCGAGGGGGGAAGCGGGGAGAGGCTCCGCTCGGCCCCGCGGCCGGCAGCGGCTT CAGAGGGCACCTCGGGCCCCGGAGCGCGGGGCTGTGCCGGCCCGGGGAGGTGGCGGTGCCGGGAGACCCCCGCGGCGGC CCGGGCCCTCTGGGTGAGCCCCTTCTGGGCCCCGACGTGCAGCAGAGCGAAGCGTGCGCTCCGCAGAGCGGGCGGGTAG CTCCGCGATTGCAGCAGTATATAACGCGGGTCCAGCCGAGGGAAGGAGGCCCGTTGCTGATAGGTGTGATTAGATACAC TTCAGTTATTTAAATCCCGATCTAACTCAAATATTAAAATCTAAAATGCGTGCGGGATTCCCGAACGGAGCGCTGAGCC CCGGTCCGCGCCTTGGCCCCCGCCGGGTCCTGACACCGCGGTTCAGCCTTTGGGTCGGTGGGGGGGGTGGGGGGGGAGC AGAAGGAGGGTTATTTTGTTTAATTTCTACAACAAACATGTCCGCAGTAGTAGATACTAAAAAAAAAAAAAATGTTGGG AGTGTGCGATTAGGTGACAATAATATATGTTGAATGAATAAGAGATAAGCTGCAAGGGGGGAGAGGGAAGCAGTAATTA CAAACCCAATTCATTCAGCTTTTCAGGACTTTTGTTGTGGGAGCTCCATTGACGGGCTTCGAGCTGCCAGTTTTCTCAC ATTAAAAAGAACTGATCAGCGCCTCAGGGGGACAAGCAACACCGCTTAACTGCATAGTGATGGGGTCCTCATTTAGAGC CATTACAACGAGAAAATTAAACAGAGCCTTTGTGTGCACCGGGTTTACTTTTCAATTGCTGACAGTTTATAGCTAACTG CTCAGAGATTGAAGTTTGATTGCGGTTTGATCGCCCGCTAGTTAGCACCGCCGCTAGTTGGGTTTTGGTAATTAAAAGC AGTTTTCTGACGCTCGCCCGGGGGATGCGAGAAGTTTGGTCCTGTTTTGCTTTTGAGGAATCGAGTTCACTTGGCGAGG ATGTCCGGCGCTGAAGTGAGTGCGGAGGCTTTTTCGCTTACAAACTTGGGGGGGTGAAGAAGCGGGAGGGGGGAGGGGG GAAGAAAAAGAAAGAAAAATAAGGAACACAGAAATGGAAATTCTCGGAGTTGTAAATGTGGCTGAGAACGCGGTGAGAA GCGGAGAGGCGCGGAGCGGGCCGAGCTCGGAGCCCCGTTTGGGCGCAGGCCCCGCTCAGTGCCGCACTGCGGGGGGAGC GGGGAAACGAACGGGGCCGGAGTGCGGGGCGGGGGGCACTGCGCTGCTGGGGGGAGTGTGGCTGAGCCGTGGGAGTGCG CGCTGAGTGCGTGTCAGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGCGCGTGCATGTGCGTATC TGCCCGTGTGCGCCTGTGCGTGCAGGAGGAGGGGGCGTCCAAAAGAGCCGTGATTTTTCCTTCCCACTTGCAGAAGTTC AGGAGGAGATGGGCTCGGCCGCACCGCACGGGGCGTTCCGCGCTGGGGGTCCCGCGCGCCCGTGGAGCGCCGTGGGCGC AGCCGGGGCACAGCAGGGCCGTACCGGGGCAGTCCGTGCCCCAGTCGGGCTGCGGTTGCCGCCGTCCCGCGCCTCGCAG GGCCGTAGCTCGGTGCACCAGCTCCTTTATAGCGCCTTTATATTTCTTCTGTTTTCTCGTCATTTCTTTTTCTTCTTCT AAGCTTTTTATTTATTTTGTTTGCATCCCCTCCCCGGTCCGAACAGGAGGAAAAAAAAAAAAAAAAAAAGAGCCTCTCC TTCCTTCCAAGTGCCCCTCGGCCTCGAGTAACCGAGCGGTTATTTCAAGGCTGTTGACTCCTTCAGGAGAGCCAGGGGA TGGGGGGGCCGCCCCGTCGGGCGGAGCTCGGTGGGCCATAGCGGCGGTCCCCGGGGGGCTCTGGGGGCTGCGGAGCCGG GCTGCGTCCCCACGGGTCCCCACAGCGCGTCCCTCCCCGCTGAGCAGAGGGGGGGCTCGGGGCAAGCCCGGCCCCCGCC GCCCCCCCGCCCCGGGACCTCGGTGACCCCATCATCGCTCAGCGGTTAGGGCTGCTGACGTCACGAGGGCGCTTTTTGC AGACAGACGTGATGAATGGTGTGTGTGTTGGGGGGGGGGGGGGAACCCCACCGGTCTGTGGAGAGGAGCGGGCCTCTGC CCTACGCTGGGGGGGACCGGGCTGGGACCCCCCCAGATGGGGATGCTCGGGGCTGGGAATGAGCCCCCGGAACCAGGCG GGAGAGGGCTGCAGCCCGTCCTCCAGGAGCGATGGGTCCTTTAAGCCCTTACAAATTGCCAAACTTGCGGAAGACAGAG AAATAATTAATGTATTAAAACAAGCGGCGAATTCCTCAGGAGGCACCTTATCAACTCCAGCCACACTGCTTCATCCCTC CTCGTATGCTAATGCTCCGTGACGTCACCGGCGCCAGATTGAATGGGAATCAGTTATGAAATTATTAGTTTTAGCTCAA AAAGCAATCTGCCGCTCCGCGAGTGGGGCCGTCGCTCCGCGGCCTTCGCCCCCGTTCTCCCCACGTTACGCACACGCTG CGCTCGAGGCTTTTCCCATCGCAGCGGTCCGTGGTCCCAAAGCACGCACCCGAGCGGGGTTTGGTGTAAAGAAACCGAT AGGTTCGCGGGGTCCCGAGCGTGAGCCGGCAGCGCCTTATCGGTACCCGCGGTACACCCGACAGCAGGCAGTCGGATTT CCTCCTGCTGGTCAGGTAGGAGAGAGCTCCTCGGGATTTATGGCCGCTCGGTCCCACGGAGGGGCTGCGCCAGGGTGTG AGGAGCGGCAGCCAGGAGCGTGCATTGTGGTCGGAGAGAAGCAAAACGGGCCGCAAAGAGGGGAGAAATGTATATATTT ATACCGTCGGTTTAAAATAAAAAGATTTCTTCTCTCGCGTTTGAAAAGGAGCGTAACCCAGCGGTGCAGGGCCACGAGA GCCGAGCGGGGAACAATGGGAATTAACACCGTTCCAATCGCTGAGGCCACTCTAATTAGATGTCTGGATCCGGCGAGAT CAAATGCCTGAATCTAAAACCCCGGGCTCGGTTTCTTTCGCTTGCAAATGCTTCTGAAAAACGATTTGGGAAGAAACAA ACCCGACCGTTTCCCAGACCCCCCCCCCACCCCCCGCCCCGAAGAGTTCCGCCGGCCCCAACTTTGCCCCCCGCTGTCC CCGGCCGCGCTGTGTCCCCCCGGGTGGCCGCGGTCCCCGAGCTGTGCCCCGCGGCGACACGGGGACGGGAATTGCCGGC AGCGCTCCGGGACGCTTTGCTCTCTTGGGCCCTTTCTTCCCGTCTCCCGAGAGCTCCTCTGCCATGAATTATGGATCAG CCCCGGGATCGGCCCCGCGCTCCCGGCCCGGGGCTCCGGGGGGCACCGAGGCGGCACTGCTGCGGCACTCCCCCCATCC CCGTCCTCCTGCCGTCCCCTCAGCGCACGGGGACGGGAAGGAGAAAGGGAAAAAGTTGTGACTGGCTGAGTGAGGAGTG AGCTTTCTGACTAATGAAATCCGAAATGAGTTGCGAATATCAAGTATTGTAAGATTTCTTCCCTGCCCCCCCGGCACTG ATTGGCAATACTCTTCGTTTTTTTTTTCTTCTTTTTTTTTTTTCTTGTGTGTTGGTTTGAAAAGCAGGAAGAAATGTAA TGAATTGAGTGCTGTCAGAGATGTCTTCTGTGTTGATGTGGTGAATGGCAGTAGCTCCCTGATCCAGTGCAGGTGCAGT AGAGCTGGGGAATAAAAGAGGGGGGCTCTCTGTTCAGTGGAAAAAGCACATTCCCTACAATAGACCACACACTGCCAGC CAGCACGGCATCAGCTCACACACAGACTTTTTTCGCCTCTTTCCACAGTCCCGAGCTCACAAAATGGCTATACATTAGC CCTAGTTAGAGTTTGCTTTTAATGAACAGTTGTGACTCCTTTTGACCAACATTTAAATCTGCTGTGCTCGCTCTGAAGA CAGGTCTGTTTTGATCACAGGTTTTACAGACTGGCCCGGTGCCTTCCAAGGCGGCTCAGACCGGGCCATCAGCCGCGTC CGATTGCAACAAGAAAAGGAGAAAAAGAACAAAAGGCTCCCGATTAATAAATGCCTCCTCCGAGAGCTCCGAGCAGGGA AAGGGTTTTTTGGTGTTTTTTTTTTTTTTTTAAATATATATAATAAAATGGCGTGGGGAGGAGGAGAGACACTGTTTTT AAATGGATGAGATGGTCTAATCCTATACACGAACGCATATTAATCCTGTTTAGAGATAACCTGTCTCCTTTTTGGCTTA ATGTATTATCCTTATACACGGATAGAAACTTTGCGGTCCTACTAATTCGGCGCAGATTGGTTTTTCTCGGCTTCTTTCC GTCACTTTATCATGATTATTATTATCGGTTTTTTATTCCCGGCGATCTGTTCGGAGGAAGCCCGATGTTACACACTCGG TTCCTCGACTTTTATTCCGAGTGACTCGCACGGTGAGGGCTGACCGCACAGCCCGGGGCCTCTGGCTGTACCCCCCCCT TCCTTCCCCCCCCGTCCTACCGCGGCCCCGCACCGCGCCGACTCCTCAGGGCGGCCCGGAGCAGCCCCCGCGCCCGCTG CTTCCTCGCACCCGTCCTGCAAGAAGCACGTTAAGCTCTTTTGGCTGTAGCTGCTGATGCATTGCGCCGGGGAAAATTG CTCTGTGTTTGCTTCTAAAGAGCCACGTTAGTGTGAAAGTTCTTTTTTCCCCCCCTAATCGTGGTAACAAATAGCGTCA GCTTCTGGAACGGTTTGGCAGAGGCACTCTTTGCCTGATCACCGCTAAACGGGAGAGGCTGACAGCGAACCTGACCCCC CTCACACACGCTGTAACGCGACGTGGTCGCGGCAGATGACGGTGGAAATGAGGCAGATTCCGGTGGGTGCTGCAGCGCG TTCGCAGGGGTCAGCTCTCGTCACCCCGAGCACAAAAGAGCGGATCTTGGCGAGGCGTAAGGGAAAAGATAATATTGAT ACGAAAAGAAAAAAAAAACAAAACAAAACAACCAAAAAAATACGGACGGTTCTCACTTTGTTTTCGGGACGTTTCCAAC GGGTCTCTGCCGCCGGAGCATCACCCGGCAGCGGAGGTGGGAGCCAACAAGTGGGAGCCCCCCCGGCAGCCGCCCCCGG TCCCGCTGCGTTGCGCAGCGCCGCGCGGTGCCCTCCGGGCGGTGCCGTACCGGGGGCGGCCGCTCCCACCGCAGCCCCC GCGCCGCTCACAGCCTTTGTGTCCCGGCGCTGCGCACACCCGTAATTAATCCTCCTGCTTACCGCTTACATCCCGGACC CAAGGGGCAACTCAAACCTATTTAGATCGCCTTGGTTGGTTGCACAAATTTAAGTGGCAAATGAGTTATAAACCTAATA ACCGCTTTAATAAAAATAATTATTTGATTATGGCGGAGTACGTTTGGCGGAGTGCAAGGATGGGTCTGGGTCCGCGTTT GGGTGGCGCTGAGATTCGTCTTCGGGTTGAAATCTTTTAAAAACACCGTTCCAAATAGCGACAGTAGTAACAATAATAA TAACAACAAACCTAACCCAACCCCAGAAGCCCTCAAAATGCCAATCCGAAACCCAACTTCGCACCGACCAAACGGCGCC CGATCCGCCGAGCGCTGTGCAACGTTTTCTGCGATATTATGTAGGACGGATTTGTAAACACAAAACCAGTCCGAGGTTT GGGTTGGTCTGAATTCGCTGCAAAGCGCCGACACGGGGGCCCGGCCCGCCGGCACCTCGCTCACTTCTCTCCCGTGGAG GACGAAGTTTGCGCCTCTCGCAGTGTTTGTGCGCTCCGGTTTTACCAGCACAAGTGAGGATGCGACAAGCCCGCTCGCG CTGTCCGCTCTATACTTTTAACGCTAGCATATTGTTTGATTACTAATTCGAGTTAATATGATCTTTATGGCAACATAAC AGTGGATAATTGGCCCTTTTTCTGAACAACAATGTAAATCACAATCGCTTTATTATTTAATAACGTCAAACGCTTCGTT GGATTGGCCCCGACAGAGAAGACCTTTTCTTTCTAGGCAGTGTTGTTGAGAAAGAACAATGCAGATCTAATTGTGGAGA TTTCAGAAGTTGGCTCCTGTAAATGAACAGCTTAGATATGGAAGTTCCAGCAGATGTCTGCACTTTTCCCTGTGACAAA CCTTGTAACCCCCCTTCCTCCCCCACTGCCCCGTCCTCCCCCCTCCCCGACTTATTGAAGATTTTTTGTTCTCACATCC TAGCAACAACCTAAATCTTCCCGCAGCAATACCCCCTCTCCCCTGGTTGCTACCCAGCCCTCAGCTGGCAGCCTCCGAG CTCCAAATTAGCGCCTGGCCCGAGGAGCGTTCGCTGCTCGCCCCGTGCTCCCCCCGGGCAGCTGCGGGCGTTTTTCGGC TCGGCGGAGGGGAGGGGAGGGGGGCCCGGGGGGGTTTGGTGTCTCGTCACCCTCCTGCCAGCCCCGCGGACGTGCCCGG GGCTGAGTTTGCAACAGTTTGGAGGTGGCTGCGAGTTGCGCAGGGAATACGGCGGGGCCTGTCCGGCCCGGGGGTGTAC GATGGGACGGAGCCCCCGGAGCCCACCTGGGGGCTGCCCCCGCAGCTCTGCGGGGCCCATCAGCTTGGCCTGCCCCCAA CACCGGCCCAAACTCTTCCGGCTGCACTCGCGGTGCCTCTTCTCCGGGGGAGGAGTCGGGGACCTCGCGGGACGGCTCC GAGGGGAAGTTGCGCTGTTCCTTGCGCGGCTCTGCGCGTAGCCCCGATCCCCTCCGAACCCGCCGGGCTGCGCGCTTTG GGCGCTGCGCGGAATTTTCCCTCGCGCTTGAGGCTGGGACGAGCCCTGCGCGGCAGAGCCCTCTCCCTACCGCCCCGCC CGCTTCTCAGAGGGCAGTTCTGCTGGGAAGAGTGATCTGGAAAACTTTGGCCGAGGGGAGCTGGCAGCAGTGCCGAGCC CCTGCCCCGTCCCGTTGCCCCCCCGGGCGCCGAACAATGCGCGCTTTGTCGCGCGCGGGGCACCATCAGAGAGAGATTA GGGCGATGTGTGCCGGCCCTCCGCGCAAGCAGGGGTGTCAGGAGATGGGGCAGAGATACAAATACGGCAGGTTCTCTCT CTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACACGAAGAGGCCGGGAAAACAG TGCCTCGATCCCCTTCTGCAGCGCCTCCGGTGCGTTCCCACCGCCGTGTCCGAAGCTCCCGTTTTCAGCCCCAGGAACG CGGGCCCGCAGCCCCCCTGCCCTGCCCCGGGGCGGTTCGGGTTTGTTTGGTTACGAGAACCCACTCGAGGGAAGTGGTG AAACAGATCGCTGAGATTCCCCGCAACGAATACGGTGAATCGGACTCACAATCCCTGCCTACAGCCGTGGGAAGGGACT CCTCGGTGCCTATTGACCTTTGGAAGGGAAATCTTGATTTTTTTTTTATTTAGTTGTTTGTTTAGTTTTACCTTTCCCG GCTTGTTGGTTTAGTTAGCAACTTTGTCATCACCTGACGCGGGCAGCAGCGCGCCCGCCGGGTGCTGCCATGGGAGCGG GTGGGGGCCGCGGGGTCGGGGGGGGGGGGGTCTGCGGGGCTCTCCTGGATGGCAGCGGCGTGCCAGGGGTTAAAGGCTC CCGCTGTGGGGCCGTGGGTGAAACTTCCCCGAATAACGGCAACGGGACGGAGCCGCCCCCGAGCCCCCGGGCTGCACTG GGCCGGGTCGGGCCGGGCCGAGCCGGGCCGGGGGCGGGGAGGGCTTCTGCTGCCGCCACTCCTCCCCGTAATGAGCTCA TTTCCATGTCTGCACCTCCCGCAACTGTGAGAAAATCCGCCGGACCAGGCAGCGTGCCGATGCGGCCGGGGCTGCCCCG GTCTCCCCGGTGACAGCGGCCGCCCGGGAGAGCCGCGCCGGCGGGTGGGAACAAAATAAAGAAAATAAATAAAGAAAAA GAAATGCCCCCCCTCCCCACTTCGGCCCTCCGCGGCTCCTCGCGGGGCGCTGCTTCCCGCAGGGAGGGGGGATGCGTGG GAGGACGGGGACGCGGCCACGCGTGGCTTCCGTGGGGTGCGCCCCGAAAGCCGGAGCCCGGGGCGGGGGTACCGGGACG GCGGGGAAGGGGGAAGGGAGCCCAGAGCCCCGCACTTATCAATAACAAGATGTATAAATATCAAAGTGAAGGAGCCCGA GTAAGTCTTTCTAGAAGCGAGGAGGAAGCTTAAGCAGCTTTCTTTAATGGTGATTTGTAGCTCTGTAAGGGGCACGGAT AGCAAATACCCTGCAGGTGCATGTTGTAACACTGCCATCCGGACTTTAATGTAGATTACTCTCCAACTCGTTTGATCGG TCCTTTGAAACCCTTTACAATTGCCTGTGACGAACCGCCAGTCTCAGTTTTTTTTCTTTGAATAATGCCTTAAGGGTAA GTCCTCGGGGCTTTTAAAGATCTCCAGCACATAGAAAAAAAATCACGTTAGATTTCACTTTAGAGCGTGGGTCCGCGTC TCTCAACTCAGAGCTGTTAACAGGCATCTTTTAAATCATTAATCGTTTAATCACCACCAACTTTACTCGGGAAGGGCGA TGCTCGCGGGGATCTTCTAAAGCCCTCCGCACATCTGCATCGCTGCGGCTTTGGGAGGCGGCCGCCCCGAGCTGCGGTC CCCCCCCCCACCCCCCCCGCAAACGCGTGCCGGGGGCTGAGCCCGGGGAGAGAACTCGAAGGGAGGGCCGGGGGGGTCC GCTCCGCTCCTTCCTCCCCCCCCCCCCGCTCCCAGCAAACAGCGGCCGGGGGTGGGGGGCAGAAGGGACGAATCGCAGA GAGGGGACGCGCCGAATCCCGCAGACGTTTCAGCTCTCCCTGCACGTAGCGTTTAACCTTTCATATTTATTCGTTACTA CGAATTAAAAAAAAAAAAATGATTACTGAACTTTTGGCCCTTCCGCTTCCACAAGCGGCGCTTTGTTTCTACAAAATAA ACTCGGGAGAACCAATCCCTCCCGTATCAAACCGGGGCTCTGAATTAAAGGCCCATTTGGGACACTCGAGACTACAAAG GGTTACACATAAATAAATGCCACATTTGCCTATTGCAAAAGTTTTCTCTGTCCTTTCTCAGTCGCAACAGGGGAGGAAA AAGCCGTCGTTTATTATCTCGCGCCGGGCAGGAGCAGCCCCTCCAGAATGTGCTCCGGCAGAAGAGGCACTCTTTTTCC CCCCCCTCAACAGCAGCTCCTTTTGTTCTCCAGATCTCTTTCCCAATTTCAAGCTTCACTTGCGGATCCGAAGTTTGTT TGTTAATGACGCTATTACCATCTGGCTCGTCCTACTTTCCAAAAGATATAAATCTCCCCGGTTTCATTGCCCACATTAT CATTTAAGTGCCACCATCGCTGGAAGAAAAATACGCTTTCAAGGCAGCGGGTTCTCCCGCACGCCCAGCGCCGGCCCCG AACGCGCTTCCCCTCCCAGCGCCGCTCGGAGCGGGGCACCGCCGCGCCTTCGGTTCGCCACCGTCGGCTTAAGTGAAGC TCTCCCCCCGATGTGGGGCTGCTGAGTGCATCTGGCTGCCGGGAACCGTTATCTGGGGCGGGACGGGTCCTGCCCCGGG CCGCCCCCGCCCCGCACCGGCTCACGTCCCGTACAGACGCGGGGAAACCTTTCCCACCGGCGCCGCGCAGTGATGGGGA GAAAAGTTTGGGCTCCTCCCGGAGCGTAGCGGTCGCGGATTCGTGTGCGCTTGGGCATAAAGGGCCGGGGGGAGGGCGA GAGGGGATGGGGGAGGGAGCCGGATCGGTTTGTGGATGGGCTCCACAATGACTCTATTTATTCGCTGCGACCAGCCCGG GCAGGCTGAATAGGCGAGAGAGGCACCCGGCCTTCCCAGCGCGTGGGCCCGGGGCGACCGACTCCCCGCGTTCCCATGA CGGGGGTCTGCGGGACGGCCGCGCCCGCACTGCCCGAACGGCCCGCACGGCCCCGGCCCCTCTGCTCAGTGCATTCAAT CCTTCAGTCGATCTCCATCGCTTCTTTGCTGGGTGTCCGGCAGCGCTTTGGCGAGGTTTTATTGAAATACAGCCGTACA CACACGCCCTTAATATTGCCAAGTGAGGGAAAAGGTACTCCTCTAATACATGAAGGAAAGAAACGTGGGGATTTTTTTT TTCCCCGAGGGATCTAATTGTTGTAAACAATTACGGATGAAAAGAAAACTCACTGCTGATGCACTTGCAGAGCTGTGAG TGCCTGGGAGAGGACGGGGCCGAGGCAGGGACTCACAGCGCGCATCGCATCGCATCGCATCGTGTCGTGTTGGGCCCTT CGCCGCCGCCACGCAGCCACGGCCCCGCACAGCCGAGTGCAGCTGGGAGGGATCTTGTTTATAAATTTATATGACGACC TTTCCTTTGTGTCTGCTCTAAAGTTGATATGGACAAATTGCAGAGGTATACAATTTAAGTAGATGCACTTTTAATCACC GATCATTAAAATGGTTATGTTAAATATACTCAATTACATGCATGACTTCACCCTAACAGTACTTTCTATTCTCCCAGCT GCTCACGTATTCCTTAACTATAGTCAGCAGAAGAAACTCGATTATGCTGAATCCATACATTTCTATGGATTTCATCAGC CTAATTAATGCTTCAGTGTGGTCCATGTGTCTGAATGGTCCCTTTGCTATTACTTGGGTAAGGAGACAGTGCAGGAATG CAGAGGGGCTTTCTTTCAAAGGTACCCATGGGGGTCACTCTAGGACTCTAATGGCAAAGGTAGCAAGTGTTAAATGGCA GAAACATGCAGAGAAAGGGAAGGCGACGATGTAAAAAGGACACATCGATACTGTAGGTATGCCCAACAGGTCAGTGATG ATAATTAAAGTGCTTAAAGAAGAAAAGGTAAACGTAGGGTCTCCAAGACACGCAACGTGCCTGTTTGAAAGCAAACACA TCAGCCCACATGGACACACCGTGATGGTCCTAGTGGGAAGAAAAAGTTGGCAGCAGATTTTTTGCTTAAATAACCACCA GGGCAGTGTCTCCTGCCAGCACCAGGTTGGAGTGCCCTGGCTGTTCTCTCATTTTCTTGTTGTTGAAATGCAACGTGTC CTGGAGATGCCTGTGCAGCCGGGAAGAGCTGGGTGTTCTCTCATGGCTGCATTGTGTGCAGTTGTGGTCCTGGCAGCGT TTGGGGATGTTCCTGCAGCCTCTGAGGTTGGTGTGCTCAGGCAGTGCAGGTGCACACGGAGATGGTAGAAGTACCTCTC CTCTCTCCCACCCTTCCTCAGCTTTCTTCCAGAATGAAGAAGCAATTCAAGTAAATGGAATGCACTGCAGCTTTAGCAC AAGCAGCCAATGGCTGCTTTAATAACACATCTTCCTATCTCAGCATTTAGTTGCATCTCAAAAGCAAGTGCTGTCTGCT CTTCAAGCAACCTGTTAATGAATGTGAAATTCATCAGGATGGATTTACATTGTGTTCCCACCTGTTGTGCCAGGAAACC ATCAGATCAGCCCTTTCTCTGGCTGCTGGGAGCCATGATGGAAATGAGAGACCTTTCTTTCACCCACTGCCACGCCAAG GAGCCCGCACCTCTCCCCTGTTCCCCATTTGTGCACCGTGGCTCAGTTGTCCTCACTAGTGTCCACGCCTCTCTGCAGC CTTCATCCTTCAAGTGGAGAGAGAGAGAGCAGGAGAGATTTCAGATCCATAACCAAATTATAACCTAGGAACTTTGTGC TTGTCATGCTAATTTAATGACTTTTATAGTAAGTGTAGACTTTCTGTCTTTTGAAATTAGCACTTCAAAATGCTTTGTG TAATGACAGACACTAGACATAAGAGACAAATGCACAAGCAATAGTGGGATAATAATCTGGCAAACACTGTGCCATTTAA TATGAAGATGGAGAAGGAAGTGAAGCACGTCTCAGCGGAGATGTTTGTCACTGCCCGGGGCTGTTGGTCCTCGTCTGTG CTTCGGACTGACCCAAAATGAGGAGGTACTGAGCGTGATATGGCATTTCAGTGTGATGACCCTTTGCCTTCTGGTGGTA CATACACGTGTTTGAGGGCTGTGAGCAGTCTGGTTTGACTGGGCTGTGCCTGGGCCCAGCCCCTTTGCTCGGATCCCAG TGGGGCTGTATGTGTGGTGCTCACATGTGTGGCCCTGAATGTACGGATCACCTCCATTCTTCAGAGCACTTCGGTGCTC CCATGTTGTGAGCGGTCCACAAAGCTGCCCAAGAGCTGGTCTTGATAACAAAGGGAGGGATAAACCCCAAGCTATAGGT GTACTGCAGTCTGGGTAACTCTCTCATTGCCTATAATTGTAAATAAAGGAAGTTCACACGACGATCGAGTGACGGGGGA ACAAGAGGCCCATTTTAATCAGGTGGTTATCTCTCCTTGGTGGAGATCAGAGCTCCATCGCTCCTGGCCTTCTCATTGT GTCCCCCCTGCTGGAAATGACAGCTCCTTCAGAAATAAGCAACTTGGTCCCTGGATGGGATTTCAGCTCCTTTGCACTA CACATGTACCAATTGTGATCTTACAGCTACAGCGAAACCTCTAAATTATATACGTGTATAAACTCGGCCTCATTAAATC CCTCCGTTTGGATAGCCTCAAGGTTGGAGAGTATATTACTGTAAGCCAGAGAAGACTTAAATTATATCTAAATGGGGAT TGAAATGTAGTGGTACTTAAGCACACTTCTTGTCTCAGCTACAGAGGTTTCATTTTTTCCCTTTAGAGTGTTATAATAT TACAAACGCAACAGTTTCCTATGTCAAATCAAAGGATCTGAAATCCCGTTTCTTGATTCAGCCTGCAGGTCCCTTTGTG GTGGGGACAAAAACACAAAGATACGATCAAACACTGCTGTCTGACCAAAGAGAACTTATCGCTCATCTGAGAGAGCTTC CTAATTTGAAGCTAAAGCCTTCCCCTTCCCGAGTGCATGTGAGCTGTGTGCTGCCTGTGTTGTTGTCTCGCGTGTGTGC GCAGCTAAACAGGGCTGTGCAGCACGGGATGGGGATTGAGAGCAGGGTTACCTCCCAGCTCCACATCCCCTGCCCGCAG GTTGGTCCGTCCCATCAAAAAAAGGAAAGGTTTTAGTGGAATATCTCTGCGTGTGCGGCTGCCCTGTTTCTGCTGAGCC TGTTTACAGTGGGTGCCTGGGGCACGGCGGGGGCTGAGGGGGCTCTGCCGACTGTCCCCTGCAGGTCAGCTCCGTGGTC CCTCTGCCCGGCACTGCCCTCTCTGCCCGGCACTGCCCTCTCTGCCCGGTGACACTGATTTCCCCGCACAGCTCCAAAT CCATCTCTCCGAAGTTGGGGTTGCTGGGAGGGGCTTGACCAACCCCCCCGCTCCCCCCAGTCCCTTTCCAGCGCTGGCA GGAGGGTCAGTGGTTTAACCAGGCGTCACCAGGACTTCGTTTTATCCCCGGCCCACTGGAGCGTGTGGCTTTTAGTGAC TGATGGTCACAGTTGCTCTCACGGTGCGTGCTCCAGATGGGCACGTCCTGGAGGCCGGGGGGGGGTGGGGGGCGGGCGG AGGGAAGCTTGTGGGGGGGAGGACGACACGGGCGCTTCGGAGAGGCCACGATGACACCGGGATTTGTTCTGGGTCACCC CCCCACCATAACGACAAAGTTGAGAAACTTCTACAGCAGTGCAGGAGCGAGTGCGTGTGTGGGGGGGAGGGGGGGGGAG TGTTGGCGGAGCTGGGGTCGATGTGCCCCCCCCTCGCTTCGCCCTGCGCGTCATCCCCGCGCACAGCCAGCACGTTCCC AAACTTTGAAATGCCAAATCACGTTATTGCTGCGGCGGGAGGCTGCTTTCGGATCTGTGACAGCTGAGTTTTTCCTATC GATTATGTGACACTGCCGTTCATTTACACAGCTCAAAAGCTTAAAGCCACGGGAGGAGCCCCCAGTTGAACCCGAAATC CTAACAGAAAGCGAATCAACTTTCTGGCCGCTGTTTCAACTGATAGATCAGAACTTAATTCGGAGAGACAGAAAGGGAG AGAAACGACAGCACAGATTTATCTTGCCCGGCGTCTTCCGCTGAAACTTTAGAAACTGCGGCGTTGCAAACCCGGGGCC GAGATGCTAATGAGCGGCAGCGCGTTGTGTCCCCGCTCGTCATTTATTGCCGAGAGCGGATCGCGGCGCTCGGGGACCC GCGCGGGTGCGCGGGCGGAGCAGCGGCGCTGCCGGACCGGGGCCGCTCCGAGCTCATCCCGGCGTCTTTCGGGGAATGG GGGACACTTTGCTCCCCCCCCCCCCCCGCCCCTTCCTTGCGATCCCTCCGTGCTGGGTCTGCTGGGGGCTGTCCAAGCG GCGGCCCATCTCTGGGTTCTGCCGCTCCCTCCCCACCCCCCGTCTTCGCCGAGGTTTCATTACTTGCTGTTAGTGCCTG GAAACAATAAAGGATGTCGGACTGGGCTTTTATTGATTATTTGTCACCCTGTATTTGTCAGTTTCAATAACTTTTCCTG AAGTTTTACTTACGGGCACTTGAGCTTTCGGATTAGGCCAGCTCTGCGGATTGCAGCGAGGGGTAATTATCCGCCGGGC TGCTTCGCCGCGGCCGGGGGGCGACGAACAGCAGCACGCTGAGCGGGCAGGATGCGGCCGGAGCCCCCCAGAGCGCTGC GGGGCCGAGAGCCGCCCCCGCCGCCCCCCGCCCGCGGCCACCGCTCGCTGCCCGCTTTGAGGCCTCCGATTGAAGCCCG GAGCCGGGGCTCGGTGCTGCGGGACGGGATGGGATGGGAGAGGGAGAAGGCTGAGCCTCCCCTCTGGCCGTCTTCCTCC CCTAATAACTTTCCTACCCAAGCGATGCGCGCTGCTCCATACGTGCTCCAGCTCCGCTCATTTGTACACACGGAAGGCG AGCTCGCATAATCGCTGCCGGGCTGGCAGGGTGCCTATCGCGTACAGTGCCGTGTTTGATGTGCTGTATCTCATAGCAC GGCGAGCTCGGAGACAAGTGAACACAGAGTGCCCAGTTACACAGGATTTAATTACAGCGAAATGCATTCTCCATGCTGA TTAGGAGAAAAATCAATCGGGGGCTTATTTGCCACAATGAACCAATAATAATAATAAAAAAACACCACCACCAACAAAA AAACAAAAACAATTTATTAGAGCAATAATCTCAGTCCCAAATGGTCTAAATATTATTGTTTCTATTGCACTGAGGAAAT TCATACTGAAAGTGTTACACAGGGAGCATCAGGGAGTCCTTTCCCAGAAATCCAGCAAGTTTCTAACTGATTTCCAGGG GCCTGACGCTGGCTTAGAAACCCTGGGAACAGGGGTTGGGTCCCCCATTTCAGGTGCAAGTGTTGGCTTGCTGCATCAC CTACAGGACGAGATGCTCTGTGGTCTGCAGCTGTCTGTGCCAAACCTAGCATGAGCTTCCCACCAGGGCTTGCAGATTT CCTGCTTTCTAGTGATTTATACATGGAATTCCTAGAAGAGAGGAGGCCACACAAAAGATCCCCGAGCCACAGGCTGAGA TAAATGCCTTGCTGTAACACAGCTAGCAAAACACCCCAAGATGTGCCAAAACCTTCCAGCTGGGACTGGGTGGGGAAGT GACCCCTTGGAGGGTGCTGAGCTCTGAGGCCAGGCTGGGACATGGGGGGACAACGGGGGCAGCTGTGCCCTCATTTCTC CTCTCCCACGCGGAGTTGCTCCCCGGTCTCAGCTGGACCTAAAGCTCCCGGGGGACTCCTGGAGAGACAGGGGGTGTAC AGGGGGTGCAGGTGGGCCAGGAACCGTGGGGTCCTGGGAGCTGGAAGGCTGTGACTCAGGAGGTCCTGGTTTTGCTTTC CAGCTTTCTGTAACTCCAGTCATCTCCGTCCCAAACCGGAGGGGACCTCATTTCCTGCTCAGCACTCTGCCAACCACAA GGTCTTTTAATAAGGCTGCCAGGGGTGCCTGGCTGCGGGAAGGACAGTCAGTGTTTGTCACCAGGGAGTGAGCTGGCAT CATAGCTCTAATCTGAGTCAACAACCTGGCGAGCCCTGAGCATGACTTCATAAATCATGCTATCTAATTGAAAAGCACA TGCTTGTATGTACAGGGCTGTTGAAAAAGAGTGCAAAAAATAAAATGTAACAGACACTTCTGTTCTTCCCACTGGCCTA TTAATCAGTCACCTGATTGTTTCAACTTAGATTATTTCATGCGGAAAAAAAAAGGGGAAAACATAAAACCACCTTGTTT AGCCACAATAAAAGCACAAATGAAAAAATTCATCTCTTAAGGAGAGGCTTAGCCTTTTTCTAATAATAGGAAAACAATC TCTAAGTGTTTTTAATTGAAGTTGTTTTCACGGTTAATTCTAAACTGCTGAATAATATTACCACATGTCTATGTATCCA TTTGGCACTGAGGCATATGATGTTTCAGGGCAATTTAACCAGTTCTGGGTGGAGGAAAAGTCCTTTGCAGTTTCCGTAT TCACTTGTTTAAAATGTTGGTTGGTGCATACATGCTGGCCTTCCTTGTTCCCTTCCTTCTAGTGGAAATGAAAAATGAA TCTGTGACGAAATAGCAGATCTGTGCGGTTTGCTTCTCACACACAAACCTGCATTATTTTCCACCCACAGGAGCTGAGG GATCGTGTTGTGCCAGAGCCACCTCTGCAGCCCCCTCGGCCACCTCAGCCCTGGGCAGAGGCTGAGCACATCTGAATAT TTCTATTCAACTCTGCAAACAGACGGAGATACTCCAAGGTTGTGCGTGAGTCCAGATGACGTGAAACTTTCAACCCCTA AACCCCTGTTCAGCTGTAGTCCAGAAGCAGGCAGCCCATAGCGGCCTGGGGACGCTGATGGAAGGTTGGCCATCGGAAG CCCTAAGCAGGTTTTTCCCCACTAACACCGCATGCTGGTCTGTGCTCCCTTTCATAAGGTGCTCTTTAAGTATAGGAAC ACCCAAAAAATTTGCATAAAAGCTTCATCTATTCTACAAGATTGCTTGTAAATTAAGCAGCAGTTTTCCCCTATTGTAT TAGGAAGAGAGTGCACTTTGTTTTCTTGTTTACTCAGTGCAATTCCTGACCCCTTGGTGCATTTCTGTCAGCATCATGG GACCTTGCTTACCTGGGACATGTCATAAGTGTGAAATGGGTCATGTTCAAAGTACCTGAAAAAAACAACAGTAATGGTG TCTGACAACTTTTCTTTTTCTTTTCTTTTTTTTTTTTTTTGGCTCTTGTTAAATAATATTTCATGGTGCATTAAGTGGG TTACGCAGCCTCTTCTGAAGAGACCACAGCAGCATATTGCTGCATGCTCAGTGCTGTCGTCAGGCTGTTAGTTCTTAAC TGGAGAAAAGCTGAAGGCAGCTGGAATCCCTTCCTGAGCCTTTCCTCAGTTCGTTCTGAATTCGATAAAAAATGGCAAT AATTTGGAGACAGATATAGAAAGAAGAAATAAAAGTGATTTCACAGTAAGCCCCTTGGCTTGCCACTCGTGACCACAGG CAGATATTAGAAGTGCTAAATCCATTAAATGTTGCTTTTCCAGAAGCCGAAATGTTTTCAGCCAAACCGCTCTCTTGCC GGCAGCTTCGGATGCTGTAGCGGGAACCAGAGCAGCCCAAGGAGAGAAGCAAACAATTAAAACATTTACATGTTTGCTT CCTGATAGACATCTCTACAAGGTAGGTTTTAACGTTTTTGTTTTTTTTTTTTTCCCTCTTAATTTTTTTTTTTTCCTGT GGTAAGAAGGATAAGCTTGGAGTGCCCAGTCACCGTGGTAACTGCCAGAAAAATTCTCCTGAAGTTTGAAGGGGTCAGC GCGCGGGGTCAACAACTCTGGCCTCTCCCCGCGCCGGGGCTGCAGGCTCCGTCCCGTGGCCGGGCCTTAGCGCGGGCTC AGCCCTGGGAGGTGGGCCGCTCCCGCTCTGATATAAATTTTTTGAGTCAGTTCACGGCCTGGGCTCTCCAGGTGGCCTC CAAAATCGATCGTGACCTCATGACCCTGTCTAACGGAGGCATAGCATCCAACCTAAGTAAGGATTTAGGCAGGAGCGAC AGCTTTCCGCTGAGTGTTATTGGGGACAGTGCAAACAGTTTTGGAAGCGAGGAGGTAGGTATGAGGAGCTGAGGTCATT AAAGGCATATTAAAAAACAATTTTAAACAACCTCGTTAGTGGAAAGTTTGTATTAGCATTAACCATTCCTCAAGTAAAC TGTTCTGTCAAGGACCCTTTTTCTCTCCCTAACAATATCAAATCGAGCATGAATGCAGGAAAAAATTAGCACTTCATAT ATTCCAAACCAAACTCATATTTTGATTTTTAACTTATAAACCAAAGTGGCTGACATGTGGTGAGACGGGCTCCCAGGAA GCACGCTTTTGCTTTAATGTCCTAAAACCCAGGAGATGGCTTATTAAAGCAATATGGTTATTTTAGGGAGGAATATCTC TGATGTATGTATTCTTTTTAGATTCCTGGAGCTCTTAAGTGGGGTAAATTTATTTTTAAACTGGGAAATTAAGTCACAC TCAGAATGGAATGAAAGTAGGAGAGTTTCGCAGGCTTCCTTTGCTGATCTGTAATTCAAATGGACAAGCTTTGGGTATG CATGTTTTTGCAGAAGAAAATTTTCATCTCGCTAAAAGAGAATAGAGAAAATTACAGTTTTCCTGTGAGATTTTAAACA ACAAACCATTGGGGTGGGGACAAGGGTAGGCTGCTGCAGAGGTCTGATTTTGTTAAAACAAATCTATAAACTTGTCCTC TGAGCAAGAGGATTTGCTGTCCTGTTTGATTACCATCCCTGCAAGCCCCCAGCTCTGCACACCGTGCCCACAATCACAG TCACCAGGTGAGTAAGTGGAACTGCACGGAGGAGCAAAGTCCAGTCTCCAAGGAGGAAAATGCTCTCAAACTCTGAGTG ACTTTAAAACTGCTCTGAAGGAAGAAAATTGCGTTAATGTTTGTTCCCAGTCTTGGAAAAATTACACAAACTTATCCCA GTGGGAATCCTGCCCAAAGGCTTCTTTCGCTCCCTGTGCTAAACGGCATTGCCCTGCCAGGCAGTGCCATCCGCTGCAA CAGTGGGAGCCTCTCGCTTGGACTGGAGCAGAGCCTGGCTCTGCAGAAAGCCCAGAACCCAGGGCCGCCTGTTGGCCGA GGTTCTGCCTCTTAGGGCTGCCATAATCTGCCCATGGGGGTTATCTGAAGAGCAGGAAATGAATTGGTACTAATAAGAG GGGCAGAACTGTGCCCTAAATAATGTGAGGCCCAGACCTGCACGTGCAGTATCAGTGACAACACTCACTCTAATTCCAT CCTCACTACTGCCCATAGTTTGTTGCTGTTGGTTTTTGGTAGAACTGTACTAAAACCAATTTATTTGCATTAGCAAGCG TGTTGGTTCAGGGAAGTGCCCTCTATGACTAAGCTTGGCAAGGCAGAGCTCACAGTCCTTGATGAGGATCAGCATCAGC GTATTTGCTTGGTTTTCAGAAAAAGCATTTAAAAATAGGAATTGCGAGCAGCTGCAAGGTGAATCCTCAGTGGTTTCAA AAGCAACTGCAGAATATTTAGCAGCTTTCTTTCTCTCTCTCTCTCTCTCTTTTTTTTTTTTTTTTTTAATATTCATATC TCCTTTGTAAAGTAAGCATACAGTGTCCCAGTTCCACCTGACAAGCAGGGTGGATGATCCAACCATCTAAAGCAGAGGA TCCAACCATTGCACTGGAACTTTGGACAAAATTACCCCAAACTTTCTCAAAAGACTTGGGAGGAGGAATCATTAACAGA GGCAGTGACGGGGGGGTCTCAGGATCTTTTACTTGCTGCAAATTCTTTTGAGTTGAACATTGTAGATCCTGTCCACTTT ATAATCAACCGCAGGTCTACAAATGCCCCCTGATACAGGCCCCTGGGTTTGCATCTTTTGTAACTCTTTGCCTTTTTTT TTAATTGTTCATCTCATACAGCTGGGATGTGGTGTGTGTTGGCAGTTGCTACAGTGGGTCTGCTCATCTTCCTTGGCCC CTCCAGATCTGGGAAGTTCTCACTCCCAATCATTTGATTCCATGAAATACCTTCTGAAGTGCAGGCTGCTTTCATTTGA ACGGAACGATATACATGAATGGGCTTAACACAGCGTTAGAGACGAGCAGTCGGAATCAGCAAGGGCTCTGGGCTGGTGG TTCCCAGCCTCTATCATATGGATCACTGCTAATCCATGGGGCCTTGGTGAGTTTGCTGCAGGTGGTCTGTGGAACTGTA TTAAATAATAACGCTCATGGTCTTCAATTAAAATTTTGATGAGAAGAGTGTGTGGCAGCCCGCACTCAAGTCTGAGAGC TGAGAGTGATCTGCCGGCCTGAAAGGTCTGAGGATGTCTGCTTCGCAGGACTGTGTCTCAGCCTGTGGTCCACCAGGCA GCAGAAAATATTTTCAGATTAATTACATGTGCAGCAAATGAGCACGCTTTCTCACTCAGGCAAGCAAGCAAGCAAAAGA AAAACAAAACAACAACCAAAAAAAGCACTTCAAAACAAAATGAAGAAAATAAAAGCTACAAATTAACACTGAAGCCCGA GCCTAGCTGGTGTTTGTTCCCACTCAGCACCACATTTCCAGTGGTGCTGAGTGGGAAGCACTGCTCAGCCCAGAGCAGG GCTGGAGAAGCTCTGCACTCACCGGAGGAAGCAGTGAAGGCAAGTGGGCAGCCGGGCTGACAGGTGTGCTGCCAGGAGG TGCCAAACAAATGCTGTACTTCCTTCTCCTTTTCTTCGCCTCTCCTCTCTGCTTTAAGTGTGTGCATGGCTGGCGTGCA GAGGAAAAGTCAAACTAATTCTGATGCTTTTCCCTATTCTAGGCAGACTAAGTCAGAGAAAGCACTTAGGGCAGCCTTT GCAGAAGGGTTACAATGTAGGTTGATACTTTAGGAGCTATAGGAAGTTCTTAATCCTTCACTGCCTACGGGACTGTCAT TTATAGACCTGCTATTGTCTCATCTTTTATAAGGATGTTTCCTTACATTAAGGCTCCAAAAGCAACCAGACTTGGCCAT TGTTCGCAGCTTTCAGCTTGTGGCTTAATGCACATCTAATATTTTGCTTCTGCTTTCACTACTCTCCTACACAGCAGAC TGTACCTACACACTGGTTTAGGTGACATCATTCTGAAAATATTCATTCCAGCGTTTCATTTCTTTCTTTTTTTTTTCCT CCTGATTTGTCTGCACATCTTAATTTTGCATTAGCCTCATTTATTACAGGTAACGTGGTAAAGCATGAGGAACTTCATG TGATTTGCTTCTCATTTCATGTTTAGTTCTCAGTTGAAGAATAGCCACACAAACACACGTCCTTCTTACAACAAAAACT TGTAAGTGCTAAATTTCCACCAGGTCAAATGCTTTATTTATAATCCAAATTTAAGTATTTCTTACCATATAATGGGAAT GAAGTTGGACATGAGCAAACAAAATGGAAATGCAGATTATTTTGGCTTTGTACAAGTTTGCCTGAGATTAGCTGAAATA AAGCAGGTGATCTGGTGACAGGATGATGTTCTGCCTTCCTGCTCTGCCTTGTGTGTGTTACAGACCTTCTTTGGAGCTC AGAACTGTGTCTATAAAATAATAATAATTTCACAAAGTACTTTTTTATGCCTGAAAGTTTTGCTGTCTGCAGTCACTGG TGGGCAGAGCACACAGGAACGTGTGATGCGCTCCTTCCTGATCCCGAGTTGCGCCCTGCTTAGCAGTTCCTCTTGCTGT TTAGTTGCTCGTGTAGTTTCCAGTTATGTAACCCATCTCATTTTGTGAATATGTGAATTTAACAGCTGTCATTGTCAGC AGTGCTGGTGTGGTTTCTTTTTTTTTGTTTCTTTTTTTTTTTTTTTTTTTTTGGCAAGCTGGCATTACTCATCAGTCTC ATGGCAGTTTTGTTTGAAAAATGCTTGTGCTAGAAAAAGTGGATTTATCAGCTCAGCACTGTATGGTGCTAGCATGCAG CACAACAAAAGACAAGTGCTCATTTCAGTAACGCTTGCAGCATTTCATGGGGCCCCCAAATAGGCTGAACAAGGCTTCT CTGGCAGCTACACTGAAGTACAGTGGGAAACTGTTAAAGTGGCAGTGTCACTGCCTGGAAAACGGCCTCTGTTTTCTCC CATCAGTTATTGCTGCTTTCTGTGTTGTTTGTTTGTTTAAAGACTATTTACCAAAGTAAGAATTTCATTGAATATTGGA GAAAAGGACACCAAGAAGTGTCTTTTTCTCACTGTTTCTTGGAGCACAGACCAAGCTGTCAGTCTGTCCTTCTCAAAGT GGCTCTGTCATGTGAGAAAAAATAAGACACTTGTGGCTTTTACAAGTAAGTCAGCCCTGCTGCAGCCCATCAGGGAGAT CTCATTCCCTGTTTAAAGCAGTTTTTTTTCAGAATTTTTGCCACACTTCCAGTTTCTTTCAGAGCTGCTCTTCCAGAAT GCAAATGCAACTTGGGTAGTGTTCTGTATTTAATTGAATGTGTCAGGTGGTTAGAGTCATGTTTTAAAAACAGTATTGC ATACTGCAAATATGCCAGACCCTTTGTTCGCAGGCATTTAAAACAAATGCATTTTTCAGTATTTATTTATTCTGTCTGA TTGCATTCCATACAGATGGAGAAGATAAACAAGTGAGTTCCATTCTGGCTTATAAATTGTCTTTTACTTTTTCAGGCAA GATTCTTCAGAGCCTGAGCGACTTCAGAAGGAGAAACTTATTAGAAGAAATCTTTGCCATTATATGTATAGTAAGTTCA CCTCCCATGTGTGCTTGTGCTCCATACAGAGCTGGGATGCATGGGAGGAATGGGCACATGCGGTTTGATCTCATTTTGC TCACCATGGAAAGGGACAGCTGCAGTGAGCTGCCTGTTTTCTGAGGTATCAGCTGCCAGACAGCCTGTAACACCTCAGC CAGAGGCACAGGGACCTCACCATAAGTGCATGCTGGCCTAAGGCCATACCTGCACATGCAAGGTGCAGATCCTTTGGAC TCCACACCTGTAGCATGGCATCCACAAAGCAGCAGCAGCAAAGCAGTTACCACTCTGTGCAAACAGCTTGCTGTGATTA ACCTATCAGGGCTCATGCTTGTCTGAGGACAGCTGGAGCTGGTAAGATGTTTGCCTTGCAGTACCCAGAAAGCTGGATA TCTCTTGGAGTGGTTCTGGATGGTTACACACAAATTGGAGACATTGGTCTCCTGCCAGACCAGGCAGAAATAACACAGC AAGATGCCTCAGGGAAGCAAGGAAGTTGTTCAGGACTTCCTCTTCCCTCAACAACAGCAAACCTGAAAGCCATAGAGGG GCAGGCTGGAGAACTGAGCTTGGCTGAAGACCTCTTGTCACTGACACTCCCATGCTCCCATCTTGGGCTGCTGGAGAGT GGCATGTCCTTCATGACTCACTGTACCCTCACAGTCATAGCTCATCTTCTGTGCCTGGCTGAGCACAGAGGGCCAATCA TGCTGCAGGACCTCAGTGTAAGATGTCGTCACAACAAGCAATTGGAAAAGCCAAAGAAGGCTGAAATCCATGGCTGTGA AGATGGAGTTGGCTGTTTCACTATGAGATGTGGAGGAAATATTTATCCTACACCTACCGCAGCCAGACTGCAGTTGTTG AGTTGAGTGACAAGGAAACGGGAGCCTGGGATGTCTCAGCACAAGCTTCCCTAAGCCTTGACTTGGTGGGTGAGCACCT GAACACAGCCAGAGCCTGTTACATTAAAGGCTTTATAAAAGCAACAGTGCCAGGCTGACATCCACCTCCCACGTTACCG GCAGCCCCAGCAGGTGTAATGCGATCCGGCAGGCGCAGCTAGCAGATAAACAAACGGCCTTTCTGCACAGGTTGTGCCA GAGAGCCTCTCCCAGCAGGGAATTGGGTTGGATGTTGTACTAGGTCAGTGAGCTGCTATGGAAAGAGCTGTGACAATGG ACAATATTGTCCTTTTTCCACACAGGATTTTCCCTCAACACTTAAGGCAAGGCAGCAGAAAAACCGTTGGGGAAAGTTT CTCTACCAGGCCTCAGAAGTTTAGTGGGATTGGGCCCTCAATGGGGGCCTACTGCTGGATTTCCCTAGGCCGTCACTTC AATGCCGCCAGGCCAGCCAGCAGCGCTGAGGGCTGCCTGCCAGAGCTGCTTGCTTGCTCTGCCATTGGGGCTCATCTCT AGTGAGTAATATTAACCAAAGGCCTTCCTACTTGGCTACAACATATCTTCCTTCTTCATTAAGGAGCTAAGCTAAGCTG CCCAGCTGTGGTAAATTTCCCTTGCTCCACAGCTGAGGTAATAATGATGCATTCCCAGGAGCAGGGCCTGCACCCTGTG TGCCACATTGGGCCTGGTGGTGTGTGGGCACATTGCTCCACACCACACCAAAATGGCGTCCGTCTGGTACTGCCCCACA GCGTGTGGGCGCACCATACCACTCCAAAATGGTGTCCATCCTGCACTTCTCTGCAGCTTAAGGGCACTCCACAACAAAA TGGTGGCTGTTCAGCACTGCCCAGCAGTGTGTGGCACTCCACAACAAAATGGCGGCTGTCCAGCACTGCCCTGCAGTGT GTGGCACTCCACAACAAAATGGCGGCTGTCCAGCACTGCCCTGCAGTGTGTGGCACTCCACACCACACCAAAATGGTGT CTGTCCTGCGCTGCCCTGTAACTTGTGGGTGTACTAGAGCAAAATTGTGGGTGAATTGCAGTGCTGCTGTAGCATATGG ACACACCACACCTAAATGGTGGCTGTCCAGCATTGCCCCACAGCATTTGGCACACCACACCAAAATGGCGGCCATCCAG TGCTGCTCCCCTGTCCCTGTATCCCAGCTGGGTCCCATCTTGCCTCCAAAATTTCCCCCTCCCTGCCGTTGCTCCAGTT CCTCATGAGTTATGATAACATATAAGCAGCTTAGAGAATTCTTATCGGAATGAGTTGAACACAATGCGACGTTTAACTC TTAATGACAGCCACCTCTGACTGAGTGACACATTATAAATTATGTTTGAACTGGGTGTTTTTTTTCAGAGGCAGATATC CCCCTGCCACCCAGCCAGCCCTGCGAAGTTGCACTTGTGGCCTTAATCCCACTTGGATGTGTGCCCCGCTGCCTCCACT CTCCGTCACTCGGGTGCTTTAGTGTTCTTGAACAAGTGAACAATCTTTGTTTCTGTACCTTATTAGGCCAGAGACCATA GCAGCGAGCGATGGCATCAGCAGGCCCCGCAGGCAGGATTCTCTGCAGACACAGCCAACCTCCAACAAACCCCTTTTTA TCCTCCTGTTTCTAGGAAGAATTATGATGGTGGTGCCCTGCTGTTGATCAGTCAAATCTGTGGTGGGCCTAGAGATAAG TCCTGTAAGTGTGGTGTACTGTAAAACAGCGGCTCCCATTCATTAGTGGCTTGTGTGTAATAAACCACCCAACAGAGGG GGTTGGGGAAGAGAGGGAAAGAGCAGCTGCAGTGTGCAGGAACAAACAACACAACTCATTCTTGGGAGCCAAAACTTTC TAATTTGCTGCTGGGCTGCTGGGAGCACTCAAATTCTTTCTGATAATGTGCTCGTGCCCTCGGTGTCTCTCCCTGCGTT TCTGCTCTCCAGAATCTGGAATAGTTTCACTCTGAACAACAAACGTGGACGCTGCTCAACGGGGAGGAGGAGGGGAACA CAGCCACCCAGTGTTTGCAGACTCCAGCACAGCAAAGTGGTGGACTTGAGGCAAGTTCTGCCGTGGAATGGCTGCTGCA CGTATCTGCTTGTGCAGTGGGTAAACAAAGCATCACATGGGCTGCAGGCTGAGCACGAGGCGCGTGGCTGACCTATACG TGTATGAGGCATGCAGGAGGAACAGCATATTCCAAACATTAGAGCCTGGTGGGATATTCAGGAACTTCTCTCAAAAAAC TCAAACAAACGAAACAAAACCACAAATTGATTATAACTGAAAAATTATTTGGCTTGATTTCTCCTGTTTTTCCACAAGT TTCAGTGAGTATTTACATAAGCGTATTTCTACATTGCCTTGCATTGGAAACAGCTTGTAAGTATGTTCATCAAAATGTC AGTCGATTATAATGTAACTCATTGCACCTGTTTGGACCTCAGTAGTTGCTAATTGTAACTGTGCATTTACAGCTGTGAC TGTGCTGGTGCTCAGCCTCAGACCTGACTGCCCATAGGACATTTCAAACTAGAAATTAAAATCACTGCGTGTGTTTCAG AACTGCTCTCATAGACGAAACCTTTGCGCTAAAGCTTGTACTGTCACGAGAAAGGGAAATTAATTTTGGATTGATGCTA CTCAGAAGGACCATTATACCCTCGCAGGATCACAGTGACTGCTTTTTCAGTGATGTGCCAGGCTGTGATGGTGCTTGGA CTGTCTCCTAACATTTCTATAGACAAGAGCTACTTAAACAGCAAGTCCAGGCTTGGGCCAAACTGCAAGTACTCTTTAT GTAGACAGGCAAAGTGTCAGACACAAAGTTTTCCACTTTAGAGCAGGCGATCTGCTTGCTTCCTCTTCCCTGACCTGGA GACCTGTTCCAGGCATTGCCTGAGTCTTAACCCTTCAATGAGAGGTGCCTCTCTGAGCTGCCCCTTGGCCATGGTACCT AATGGGTGGAGGTATGAATTCTTCATTCAGACCTGGCCAAATCCAGTGCTAGACCTGGATGCAAATCAGTTCTACCGAA GGTTTAGGTAGTGAAGGAACTGAAACTTGCTGCTTACCTGCCATGCTGCACAGGGCCAGCATGAGACCCAGCCCTACAA GCTCTGCTTGTAGGGTTTTGGTATTTTTGACACAGTCATTTGGCATTTTGATTTCCCTATGGTACAAACAGGGGGACAA TCTAAAAATAAGAAATAGCTTTCCTGCACGAATACCTTGAATCATTAGAAACTGATGCTTTGTGAGTTTTCTGGGAATC TGTTACTGTTTTTATATTTTCATAGGGATGTAGTCATCCAACATTGTTGGACAGAGGTAAACATGAGTAACCCAATTTT GTTCTTACACTTGGAACACTGCAAATAGCTATATAGTCCTGAAGTTTTTGTATCTAACCAGATGACACTGGGGGAAGTG AAATATTTCTTATTAAACTGAGGCAAAATATGCCTTTGTGATCTATAAGGAAGTCTAATAATTTTGGGTTCAAAGATGT CTAATGTTGAATTATTAAATGACTCAATGTAGCTTTCTTTTAGAAGGGGTGATACTCATCCATCAGCTGGATCCAAGCT ATTACAAAAACTCTGCTAGAAATAGAATGAGAAATAGACCAAAAACTCAGAGCTCAGGCTTGCAAATAAAATAAATTGT TGTGACTGGTTGTTCACTAATATATGTGAAAGAGGGGCTACGTGTTACGTGTCTTTTATTATTGACAGTGTGCCCAATG CTGTATGGTAAATGTTTGAACAGTGTCACCTTCTGTTCTGCCTCCTTCACCTACAGGAGCAGCTCTGGTGGCGAAGGGG CAGCGTGAGGCACTGCTGGCCCCAGCTTCCCGCAGGTGTCCATCCGGCACCCGAGGCCTCGAGCAGCACCGTGTCTGTG TCCTGTGCCATACCCACAGCCCTGGAACCATGGAAGGAGAATGAAGTTTGTTCTAAACGTGAATTTAATATGCTTTCAG ATTTGACAGCCTCTTTTAAAAGGAAACGTTTAAGTTGAGCGAAAGGCACATTTGCTTCTCCAAATAAATGGAATTATAG TTCAAAGATC
Citation	Wakamatsu Y, Maynard TM, Weston JA. Fate determination of neural crest cells by NOTCH-mediated lateral inhibition and asymmetrical cell division during gangliogenesis. Development. 2000 Jul;127(13):2811-21.
Copyright	Published by The Company of Biologists 2000. This material is protected by a copyright retained by the authors.
Comments	As insufficient information is provided in the publication to verify the exact sequence used to synthesize the probe, the sequence below was obtained from NCBI (acc # NM_205188).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 23

Spinal Ganglia and Nerves

25-30

stage 30

Spinal Ganglia and Nerves

Entry 14

GEISHA Id	SOX2.Sanchez-Arrones.2012	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Sanchez-Arrones L, Stern CD, Bovolenta P, Puelles L. Sharpening of the anterior neural border in the chick by rostral endoderm signaling. Development. 2012 Mar;139(5):1034-44.
Copyright	Published by The Company of Biologists © 2012.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_205188.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Mesencephalon
Neural Plate/Tube

SP, secondary prosencephalon
STAGE 5+ embryo

stage 7

Neural Plate/Tube

STAGE 7+ embryo

Entry 15

GEISHA Id	SOX2.Psychoyos.2008	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Psychoyos D, Finnell R. Double Whole Mount in situ Hybridization of Early Chick Embryos. J Vis Exp. 2008; (20): 904.
Copyright	Copyright © 2008, Journal of Visualized Experiments
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205188.1 ).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 7

Neural Plate/Tube

Entry 16

GEISHA Id	Sox2.Daudet.2007	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Daudet N, Ariza-McNaughton L, Lewis J. Notch signalling is needed to maintain, but not to initiate, the formation of prosensory patches in the chick inner ear. Development. 2007 Jun;134(12):2369-78.
Copyright	Copyright 2007 the authors. Published by the Company of Biologists Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205188.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Ear/Otic Placode
Neural Plate/Tube

Entry 17

GEISHA Id	Sox2.Reza.2002	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Reza HM, Ogino H, Yasuda K. L-Maf, a downstream target of Pax6, is essential for chick lens development. Mech Dev. 2002 Aug;116(1-2):61-73.
Copyright	Copyright © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205188).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-17

stage 17

Eye

Entry 18

GEISHA Id	SOX-2.Dady.2012	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Dady A, Blavet C, Duband JL Timing and kinetics of E- to N-cadherin switch during neurulation in the avian embryo DEVELOPMENTAL DYNAMICS. Volume 241, Issue 8, August 2012, Pages: 13331349.
Copyright	Copyright © 2012 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_205188.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Neural Plate/Tube

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

Central Nervous System
Mesencephalon
Neural Plate/Tube
Notochord
Paraxial Mesoderm

13-14

stage 14

Neural Plate/Tube
Notochord
Paraxial Mesoderm
Primitive Streak

21 somite embryo
22 somite embryo

Entry 19

GEISHA Id	SOX2.Qiao.2012	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Qiao Y, Zhu Y, Sheng N, Chen J, Tao R, Zhu Q, Zhang T, Qian C, Jing N. AP2γ regulates neural and epidermal development downstream of the BMP pathway at early stages of ectodermal patterning. Cell Res. 2012 Nov;22(11):1546-61.
Copyright	© 2012 Shanghai Institutes for Biological Sciences
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # NM_205188.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Ectoderm
Epiblast
Hensen's Node
Neural Plate/Tube

Stage HH4+
Stage HH4-

Entry 20

GEISHA Id	SOX2.Christophorou.2009	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Christophorou NA, Bailey AP, Hanson S, Streit A. Activation of Six1 target genes is required for sensory placode formation. Dev Biol. 2009 Dec 15;336(2):327-36. doi: 10.1016/j.ydbio.2009.09.025. Epub 2009 Sep 23.
Copyright	Copyright © 2013 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_205188.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Neural Plate/Tube

Entry 21

GEISHA Id	SOX2.Acloque.2008	Data Source	Publication
Complete cDNA Template Probe	show AGGCAGAGAAAAGGGAAAAAGGAGGAAGAAGTTTTTTAAATCCGAGGCTCCGGCGGTAATAATAGCAGTTATTAGCATT ATTAATCCATCAGCGAAGAGGAAGATTCTGATCTCGATCCAAGTGTGGTTTTGGGGAGTCCCCCTCCCCCCTTTCATAC CCCTCCCTAGGAAAAAAAAAAAAAAAAAAAGTCCGGCTTGGGACTTCGCCGCCGCCGCTCCTTCTCCGCTCCGGGCTGG TTCCAGGCTAAAGTAGTTTGAATGTACAACATGATGGAAACCGAGCTGAAACCTCCCGCCCCCCAGCAAACTTCGGGGG GAGGCACAGGCAACTCCAACTCGGCCGCGAACAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTT CATGGTGTGGTCCCGCGGGCAGCGGCGGAAAATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGG CTCGGGGCCGAGTGGAAACTTTTATCCGAGGCGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGC ACATGAAGGAGCACCCGGATTATAAATACCGACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTT GCCAGGGGGCTTACTGGCGCCGGGCACCAATACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTG AACCAGAGGATGGACAGTTACGCGCACATGAACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCT ACCCGCAGCACCCGGGCTTGAACGCGCACAACGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCA GTACAACTCCATGACCAGCTCGCAGACGTACATGAACGGATCGCCTACCTACAGCATGTCGTACTCCCAGCAAGGGACC CCGGGCATGGCGCTGGGCTCCATGGGCTCGGTGGTCAAGACGGAATCCAGCTCCAGTCCCCCCGTGGTCACCTCCTCGT CTCATTCGAGGGCTCCTTGCCAAGCCGGGGACCTTCGGGACATGATCAGCATGTATCTACCAGGTGCTGAAGTACCAGA ACCAGCTGCCCCAAGCAGACTTCATATGTCCCAACACTACCAGAGTGCACCTGTTCCTGGCACAGCCATTAATGGCACA CTCCCTCTATCACATATGTGAAGACAAAGGGGCCGGGGGGGGCGGGGGTGGGGGGGGGAAGGAGAACTAACAAAACAAA ACAAACAACAACAACAAAAAACTTTTATTAGAAATTCTGACCTTTTTTTTTTTCTTTTTTTTCTTTTTTTTTTGGACTA TTTTTGTACAGA
Citation	Acloque H, Wilkinson DG, Nieto MA. In situ hybridization analysis of chick embryos in whole-mount and tissue sections. Methods Cell Biol. 2008;87:169-85. doi: 10.1016/S0091-679X(08)00209-4.
Copyright	Copyright © 2013 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_205188.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-9

stage 9

Neural Plate/Tube

Red

Entry 22

GEISHA Id	SOX2.Uchikawa.2011	Data Source	Publication
Complete cDNA Template Probe	show CAACCAGAAGAACAGCCCGGACCGGGTCAAGCGCCCCATGAACGCCTTCATGGTGTGGTCCCGCGGGCAGCGGCGGAAA ATGGCCCAGGAGAACCCGAAGATGCACAACTCGGAGATCAGCAAACGGCTCGGGGCCGAGTGGAAACTTTTATCCGAGG CGGAGAAGAGACCCTTCATTGACGAAGCCAAACGGCTCCGAGCTCTGCACATGAAGGAGCACCCGGATTATAAATACCG ACCCCGGAGGAAAACCAAGACCCTGATGAAGAAGGATAAGTACACGTTGCCAGGGGGCTTACTGGCGCCGGGCACCAAT ACCATGACGACCGGGGTAGGGGTTGGGGCCACCTTGGGAGCTGGGGTGAACCAGAGGATGGACAGTTACGCGCACATGA ACGGCTGGACCAACGGAGGCTATGGGATGATGCAAGAGCAGCTCGGCTACCCGCAGCACCCGGGCTTGAACGCGCACAA CGCGGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCAGTACAACTCCATGACCAGCTCGCAGACGTA
Citation	Uchikawa M,Yoshida M, Iwafuchi-Doi M, Matsuda K, Ishida Y, Takemoto T, Kondoh H. B1 and B2 Sox gene expression during neural plate development in chicken and mouse embryos: universal versus species-dependent features. Cell Growth Differ 2011 Aug;53(6):761-71.. null.
Copyright	© 2011 Japanese Society of Developmental Biologists.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

4-6

stage 4

stage 5

Neural Plate/Tube

Neural Ectoderm

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

stage 12

Ear/Otic Placode
Neural Plate/Tube
Peripheral Nervous System

Posterior Neural Plate

13-18

stage 13

Neural Plate/Tube

stage 19

Data from NCBI Unigene EST Profile
Unigene ID: Gga.601

Mouse

Emage	MGI:98364
Eurexpress	MGI:98364
MGI GXD	MGI:98364

Frog

Xenbase

484552

Fruit Fly

BDGP

Zebra Finch

ZEBrA

Zebrafish

ZFIN	ZDB-GENE-030909-1

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SOX2SRY-box 2

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Entry 20

Entry 21

Entry 22

Mouse

Frog

Fruit Fly

Zebra Finch

Zebrafish

SOX2
SRY-box 2