GEISHA - A Chicken Embryo Gene Expression Database
Browse By
- Anatomical Location
- Stage
- Gene Name
- GEISHA ID
- Multiple Parameters
Search
- Quick Search
Data Class
Enter Text:
go
- Gene Family QuickSearch
Transcription Factors
Growth Factors
Receptors
MicroRNAs
- Human Disease Gene Search
Documents
- Downloads
- Protocols
- About GEISHA
- Contact Us
Resources
- Transgenic Birds
- Model Organism Databases
- Gene Expression Databases
- Genomic Resources
- Anatomical Atlases
- Chicken Stage Series
_

RUNX1
runt-related transcription factor 1

Gene
Official SymbolRUNX1
Official Full Namerunt-related transcription factor 1
CGNC ID49673
also known asrunt-related transcription factor 1|ch-runtB2, ch-runtB2 , RUNX1, AML-1, CBFA2, PEBP2*B
gene typeprotein-coding
Genomic Map
 Ensembl
NCBI
UCSC
Gallus-Browse
Sequence Information
Genomic
RNANM_205227
PolypeptideNP_990558
Sequence Clusters/ESTsUnigene
Gene Expression
In Situ HybridizationGEISHA
EST ProfileGga.45609
Orthology
Entrez GeneEnsembl GeneGenetic PhenotypesMOD
Fruit Fly33059FBgn0003300
Human
Mouse12394ENSMUSG00000022952All phenotypic alleles (43):Targeted, knock-out(5) Targeted, other(38) MGI:99852
Xenopus100485449, 379657483163
Zebrafish58126ZDB-GENE-000605-1ZFIN:ZDB-GENE-000605-1
Gene Ontology
Molecular Function
Biological Process
Cellular Component
Links to other databases
Entrez Gene396152
Ensembl GeneENSGALG00000016022
KEGGgga:396152

Entry 1

GEISHA IdRUNX1.UApcrData SourceGEISHA ISH Analysis
Forward cDNA Template PrimerGATGGCTGGAAACGATGAG
Reverse cDNA Template PrimerATTAACCCTCACTAAAGGTTATGGGATACCTACGGGCcDNA Template Length945
Forward cDNA Template Probeshow
AAGCACGGTTAGTCACTGGCCCACGACTACTNAAAATGCATTCAGTATGGCCTCCAAACCGCTTCCTCTAGCCTTGCCG
TGGGTACCCATGTTGGTTGGGGAGTTGCTGTGGCTNCCNTNTGCCTCAACCACATCNCTTNAGTTGGGAAGGTTGGGGT
TGAGGAGTGTCGATCCTGTGGAAGCATTGGTGCAAGGGGGGTGGATGCGCGGGGGGGACCGGTCTCCTCCTGAGATCAT
GGAGAACTGATAGGACCCGGCGGAGGTTCCGTAGTANAGGTGGTACGAAGGTGAGCTGGTCTGGAATGGGCTGCCTTGG
GCCTGAGAGGAACCGGGATATGGAGGTGGGAGGTAAGTGTGGTATCTGGTGGCCGTGGACATAGCAGACATACCGATCC
CTATCCCCGAACTGACGGGTGTTGGCGTGTAGGTGAACGCTCCCGGGTAGTGCATCCGAGGATCAGAAATGGAAGGGAG
CGCGGANAAGGAGCGGTCGATCCCCACTCTGGGGTCACTGAATGCCGTCAAGTCAGAAGCACCTGAGAGGCGGCTGGAA
AGCTCTGCGGTGAGGGACGTCATGCCGCTGGCCCTGCCAGGCGATATCGGCGTGGCAGGGTGCACCGAGGGAGTCNCGA
TGGANCCCAGGTACTGGTAGGACTGGTCGTANGACCATGGCGGGGACGGCTGCACTTGCCTCGTGTTTCTGGGTTCNCG
TGGTCCATCCACCGTTATCTTGATGGCTCTGTGGTACGTGGCTACTTGTGGCGGGTTTGTGAAAACTGTGATAGTCANT
GTGAANCTTTTCCCTCTTCCACTTCTACCCACAAATCTGANGTCGTTGAACCTC
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 4
stage 5
stage 6
Blood Islands
Unlabeled
 
7-12
stage 8
stage 8
stage 9
stage 11
Blood
Blood Islands
 
13-18
stage 13
stage 15
stage 16
Blood
Blood Islands
Forebrain
Hindbrain
Midbrain
Spinal Ganglia and Nerves
Splanchnic Mesoderm
 
19-24
stage 20
stage 20
stage 20
stage 20
stage 21
Allantois
Aorta
Blood
Cranial Ganglia
Eye
Leg AER
Lung
Mesonephros
Pharyngeal Arches and Clefts
Spinal Cord
Spinal Ganglia and Nerves
Stomach
Wing Mesenchyme
Labeling along ventral portion of Aorta and in underlying mesenchyme; the AGM.
25-27
stage 25
stage 27
stage 27
Cranial Ganglia
Leg Mesenchyme
Pharyngeal Arches and Clefts
Spinal Cord
Spinal Ganglia and Nerves
Wing Mesenchyme
 

Entry 2

GEISHA IdRunx1.Bollerot.2005Data SourcePublication
Complete cDNA Template Probeshow
ACAAAAGGCACTGGGATAAAGCAGTGTGATGGCTGGGCAGAGGGTTGTAACCATGACCGTTCTTCATTTTTCACCTCCC
AAGGTAGAAGAAATCATATATTCCTTCACTGTGAAGAGAAAGTGAGTTTTAAAGAGGAAACAATGGCTTCAAACAGCGT
ATTTGATTCACTTCCTTCTTACCAGCACTTCTTGAGAGATACCAGCACCAGCCGCCGCTTCACGCCGCCCTCCACCGCG
CTGAGCCCGGGGAAGATGAGCGAGCCGCTGCCGCTGCCCGGCGCCGAGCACAGCGGAGCGCTGGCGGGGAAGCTGCGCG
CCGCCGACCGCAGCATGGTGGAGGTCCTGGCGGACCACCCCGGGGAGCTGGTGCGCACCGACAGCCCCAACTTCCTCTG
CTCCGTGCTGCCCACCCACTGGCGCTGCAACAAGACGCTGCCCATCGCCTTCAAGGTGGTGGCCTTGGGAGACGTACCC
GATGGGACGCTGGTCACGGTGATGGCTGGAAACGATGAGAACTACTCTGCAGAGCTCAGAAATGCGACGGCTGCCATGA
AAAACCAAGTGGCGAGGTTCAACGACCTCAGATTTGTGGGTAGAAGTGGAAGAGGGAAAAGCTTCACACTGACTATCAC
AGTTTTCACAAACCCGCCACAAGTAGCCACGTACCACAGAGCCATCAAGATAACGGTGGATGGACCACGCGAACCCAGA
AACACGAGGCAAGTGCAGCCGTCCCCGCCATGGTCCTACGACCAGTCCTACCAGTACCTGGGCTCCATCGCGACTCCCT
CGGTGCACCCTGCCACGCCGATATCGCCTGGCAGGGCCAGCGGCATGACATCCCTCACCGCAGAGCTTTCCAGCCGCCT
CTCAGGTGCTTCTGACTTGACGGCATTCAGTGACCCCAGAGTGGGGATCGACCGCTCCTTCTCCGCGCTCCCTTCCATT
TCTGATCCTCGGATGCACTACCCGGGAGCGTTCACCTACACGCCAACACCCGTCAGTTCGGGGATAGGGATCGGTATGT
CTGCTATGTCCACGGCCACCAGATACCACACTTACCTCCCACCTCCATATCCCGGTTCCTCTCAGGCCCAAGGCAGCCC
ATTCCAGACCAGCTCACCTTCGTACCACCTCTACTACGGAACCTCCGCCGGGTCCTATCAGTTCTCCATGATCTCAGGA
GGAGACCGGTCCCCCCCGCGCATCCACCCCCCTTGCACCAATGCTTCCACAGGATCGACACTCCTCAACCCCAACCTTC
CCAACCAAAGCGATGTGGTTGAGGCAGAAGGGAGCCACAGCAACTCCCCAACCAACATGGGTACCACGGCAAGGCTAGA
GGAAGCGGTTTGGAGGCCATACTGAATGCATTTTAAGTAGTCGTGGGCCAGGACTAACGTGCTTTCAATTTACCGATGC
CCGTAGGTATCCCATAAACACCTGTCTTGCTTGTGGGCCCTCGATGTTCATACATCACTTCCAGAAGCAAACTCTCCAG
AAGTCAGCGGTCCCAAGAGCAGTGGCGTGTGTTGGGCCAGCTGCGGGGCTGGTGAAGCGATGCCAGCGCCAGGCAAGGT
ACCCCCCCGGGGGGCTGAGCTTCTGGAAAGGAGGACATTCAGGTTGGTGGCCTGTAGGTAGTGAGGGTGGGTAGGAAGA
TATCCAGGCTGTTCCCGGGCCTGTGGCTTTCAAACATGCCCAGGTTTGAAGGTCTTCACAGCCCTAAAGGGAGTTTCAG
TTCACTTCTGTTTTTAAGACTTAAGACCTAACAGAAACTTAACGAGTTTACTGCATCTTATGGACGTGTTAACTATAAG
GAAGTATAGGAAGATTCCCAGAGGGAAACTGTGAA
CitationBollerot K, Romero S, Dunon D, Jaffredo T. Core binding factor in the early avian embryo: cloning of Cbfbeta and combinatorial expression patterns with Runx1. Gene Expr Patterns. 2005 Dec;6(1):29-39. Epub 2005 Jul 19.
CopyrightCopyright © 2005 Elsevier B.V. All rights reserved.
CommentsAccording to the authors, "To ensure the specific detection of Runx1 transcripts over Runx2 and Runx3, we have used two different probes. They encompass, respectively, the first 750 bp and the last 655 bp of the chick Runx1 coding sequence, the details of which will be given elsewhere (Bollerot and Jaffredo, in preparation). All the Runx1 patterns except those of the YS obtained with the 5′ end probe were established with the two probes. For each structures, the two patterns were similar." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_205227).
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
4-6
stage 4
stage 5
stage 5
stage 6
stage 6
Area Opaca
Blood Islands
Figure 2 includes stages 2+, 4, and 5 and compares Runx1 expression to Cbfβ.
Figure 3 compares Runx1 expression to Cbfβ.
Hemangioblasts
7-12
stage 9
stage 9
Blood Islands
Figure 4 compares Runx1 expression to Cbfβ and Ets-1.
13-18
stage 13
stage 13
stage 14
stage 14
stage 14
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
stage 18
Blood
Blood Islands
Cranial Ganglia
Endoderm
Lung
Neural Plate/Tube
Splanchnic Mesoderm
Acousticofacialis Ganglia
Figure 5 compares Runx1 expression to Cbfβ.
Figure 6 compares Runx1 expression to Cbfβ, PU.1 and c-Myb.
Figure 7 compares Runx1 expression to Cbfβ.
Figure 8 compares Runx1 expression to Cbfβ, VE-Cadherin, and Ets-1.
Mesenchyme ventral to the Aorta
Neuroepithelium
Trigeminal Ganglia

Entry 3

GEISHA IdRUNX-1.Jaffredo.2005Data SourcePublication
Complete cDNA Template Probeshow
ACAAAAGGCACTGGGATAAAGCAGTGTGATGGCTGGGCAGAGGGTTGTAACCATGACCGTTCTTCATTTTTCACCTCCC
AAGGTAGAAGAAATCATATATTCCTTCACTGTGAAGAGAAAGTGAGTTTTAAAGAGGAAACAATGGCTTCAAACAGCGT
ATTTGATTCACTTCCTTCTTACCAGCACTTCTTGAGAGATACCAGCACCAGCCGCCGCTTCACGCCGCCCTCCACCGCG
CTGAGCCCGGGGAAGATGAGCGAGCCGCTGCCGCTGCCCGGCGCCGAGCACAGCGGAGCGCTGGCGGGGAAGCTGCGCG
CCGCCGACCGCAGCATGGTGGAGGTCCTGGCGGACCACCCCGGGGAGCTGGTGCGCACCGACAGCCCCAACTTCCTCTG
CTCCGTGCTGCCCACCCACTGGCGCTGCAACAAGACGCTGCCCATCGCCTTCAAGGTGGTGGCCTTGGGAGACGTACCC
GATGGGACGCTGGTCACGGTGATGGCTGGAAACGATGAGAACTACTCTGCAGAGCTCAGAAATGCGACGGCTGCCATGA
AAAACCAAGTGGCGAGGTTCAACGACCTCAGATTTGTGGGTAGAAGTGGAAGAGGGAAAAGCTTCACACTGACTATCAC
AGTTTTCACAAACCCGCCACAAGTAGCCACGTACCACAGAGCCATCAAGATAACGGTGGATGGACCACGCGAACCCAGA
AACACGAGGCAAGTGCAGCCGTCCCCGCCATGGTCCTACGACCAGTCCTACCAGTACCTGGGCTCCATCGCGACTCCCT
CGGTGCACCCTGCCACGCCGATATCGCCTGGCAGGGCCAGCGGCATGACATCCCTCACCGCAGAGCTTTCCAGCCGCCT
CTCAGGTGCTTCTGACTTGACGGCATTCAGTGACCCCAGAGTGGGGATCGACCGCTCCTTCTCCGCGCTCCCTTCCATT
TCTGATCCTCGGATGCACTACCCGGGAGCGTTCACCTACACGCCAACACCCGTCAGTTCGGGGATAGGGATCGGTATGT
CTGCTATGTCCACGGCCACCAGATACCACACTTACCTCCCACCTCCATATCCCGGTTCCTCTCAGGCCCAAGGCAGCCC
ATTCCAGACCAGCTCACCTTCGTACCACCTCTACTACGGAACCTCCGCCGGGTCCTATCAGTTCTCCATGATCTCAGGA
GGAGACCGGTCCCCCCCGCGCATCCACCCCCCTTGCACCAATGCTTCCACAGGATCGACACTCCTCAACCCCAACCTTC
CCAACCAAAGCGATGTGGTTGAGGCAGAAGGGAGCCACAGCAACTCCCCAACCAACATGGGTACCACGGCAAGGCTAGA
GGAAGCGGTTTGGAGGCCATACTGAATGCATTTTAAGTAGTCGTGGGCCAGGACTAACGTGCTTTCAATTTACCGATGC
CCGTAGGTATCCCATAAACACCTGTCTTGCTTGTGGGCCCTCGATGTTCATACATCACTTCCAGAAGCAAACTCTCCAG
AAGTCAGCGGTCCCAAGAGCAGTGGCGTGTGTTGGGCCAGCTGCGGGGCTGGTGAAGCGATGCCAGCGCCAGGCAAGGT
ACCCCCCCGGGGGGCTGAGCTTCTGGAAAGGAGGACATTCAGGTTGGTGGCCTGTAGGTAGTGAGGGTGGGTAGGAAGA
TATCCAGGCTGTTCCCGGGCCTGTGGCTTTCAAACATGCCCAGGTTTGAAGGTCTTCACAGCCCTAAAGGGAGTTTCAG
TTCACTTCTGTTTTTAAGACTTAAGACCTAACAGAAACTTAACGAGTTTACTGCATCTTATGGACGTGTTAACTATAAG
GAAGTATAGGAAGATTCCCAGAGGGAAACTGTGAA
CitationJaffredo T, Bollerot K, Sugiyama D, Gautier R, Drevon C (2005). Tracing the hemangioblast during embryogenesis: developmental relationships between endothelial and hematopoietic cells. Int J Dev Biol. 2005;49(2-3):269-77.
Copyright"Reprinted from Int. J. Dev. Biol., 49,Jaffredo T, Bollerot K, Sugiyama D, Gautier R, Drevon C , Tracing the hemangioblast during embryogenesis: developmental relationships between endothelial and hematopoietic cells, 269-77, Copyright (2005), with permission from Copyright © 2005 UBC Press". All rights reserved.
CommentsInsufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205227.1).
StageImage (click image to view full size)Location (click to highlight)Comments (click to highlight)
13-18
stage 17
stage 18
Aorta
 
31-44
stage 44
Aorta
Blood
 
 Data from NCBI Unigene EST Profile
Unigene ID: Gga.45609

Mouse

EmageMGI:99852
EurexpressMGI:99852
MGI GXDMGI:99852

Frog

Xenbase483163

Fruit Fly

BDGPFBgn0003300

Zebra Finch

ZEBrA

Zebrafish

ZFINZDB-GENE-000605-1
Home | Contact
     
© 2008 Arizona Board of Regents
This website is hosted by the Biotechnology Computing Facility at the University of Arizona