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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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HHEX
hematopoietically expressed homeobox

Gene

Official Symbol	HHEX
Official Full Name	hematopoietically expressed homeobox
CGNC ID	5114
also known as	PROBOX, hematopoietically-expressed homeobox protein HHEX\|homeobox protein HEX\|homeobox protein PRH, Homeobox protein PRH , Hex, Hematopoietically expressed ho
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_205252
Polypeptide	NP_990583
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.3316

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	3087	ENSG00000152804		MIM:604420
Mouse	15242		All phenotypic alleles (6):Targeted, knock-out(2) Targeted, other(4)	MGI:96086
Xenopus	395060, 373590			487158
Zebrafish	30098		ZDB-GENE-980526-299	ZFIN:ZDB-GENE-980526-299

Gene Ontology

Molecular Function	sequence-specific DNA binding, sequence-specific DNA binding transcription factor activity
Biological Process	cell differentiation, multicellular organismal development, more...
Cellular Component	nucleus

Links to other databases

Entrez Gene	396182
Ensembl Gene
KEGG	gga:396182

Entry 1

GEISHA Id	pat.pk0074.g5	Data Source	GEISHA ISH Analysis
Forward cDNA Template Probe	show GAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGACCATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCC CCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAGCTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAG CCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGGCCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGA CCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGGGGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAG GAGGACCCCGAGTCCGACGTCTCCGACGACTCCGACCAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCA CACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGGCTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGG GAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAAGCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGAT ATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGATCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTAC TGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTGACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCC CCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAGGGCAGGACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTTAA TGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAATGTATTCTGTGCCATAGAGAGGGAATGGAGCATCCCGAGAGTGC TTTACCCCACCAAGAACTGCAGCTGCATCCCCTGGAGGATCCCCCCTCACCGGCAGAAGCTGTGGGGACGTTCCGTTTT GTCCTGAAGGAGGGAAAGTCCCTCTCGCATTTGGAAGATTTGCCCCAAAACTGGGGGTTCCCTTGCCGGGAAAAAATAC TAAAAAAACCAAAACCGACCCGGTTCAACCCACCGGCTTTTTTTTCTTAAAATGTGGGGGGCCCCAATTTTCATTTTAA GGAATAGGGCTTAAGGCTTCCGGGCATGTGAAGGGCCCCGGCCGGGGCCCTCCTCTGGCAAGGATGCTTAAACTTTGGT TTCACCCTGTGCTTTTTATATGGTAAAAAAAAAAAAAGCCCTG

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Blood Islands
Head Process

heart field

7-12

stage 9

stage 11

Anterior Intestinal Portal
Blood Islands
Head Mesenchyme
Intermediate Mesoderm
Liver

13-18

stage 14

stage 15

Blood Vessels
Endocardium
Endoderm
Foregut
Hindgut
Liver
Outflow Tract
Sinus Venosus
Thyroid

19-24

stage 19

stage 20

stage 23

stage 24

Allantois
Blood Vessels
Endocardium
Liver
Outflow Tract
Sinus Venosus
Thyroid

blood vessels over nervous system
roof of HB, vascular endothelium in face.

Entry 2

GEISHA Id	Hex	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Area Pellucida
Hypoblast

4-6

stage 4

stage 6

Anterior Intestinal Portal
Area Opaca
Area Pellucida
Blood Islands
Endoderm, early
Head Process
Hensen's Node
Hypoblast

7-12

stage 7

stage 10

stage 11

Anterior Intestinal Portal
Blood Islands
Blood Vessels
Endoderm, early
Liver

13-16

stage 14

stage 16

Anterior Intestinal Portal
Blood Islands
Blood Vessels
Liver
Tail
Thyroid

Entry 3

GEISHA Id	Hex.Yanai.2005	Data Source	Publication
Complete cDNA Template Probe	show CGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAGC CGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACGC CGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCCC CCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCGG ACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTACG CCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGCC GCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGACC ATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAGC TCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGGC CACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGGG GGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGACC AGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGGC TTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAAG CACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGAT CTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTGA CCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAGG GCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAATG TATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG
Citation	Yanai M, Tatsumi N, Endo F, Yokouchi Y. Analysis of gene expression patterns in the developing chick liver. Dev Dyn. 2005 Jul;233(3):1116-22.
Copyright	Wiley-Liss, Inc. 2005

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Anterior Intestinal Portal
Endoderm
Head Process

13-18

stage 17

Liver
Thyroid

19-24

stage 22

Liver

25-30

stage 25

Endoderm
Liver

31-42

stage 36

stage 39

stage 42

Endoderm
Liver
Pancreas

IHBD=interhepatic bild ducts
gall bladder

Entry 4

GEISHA Id	Hex.Chapman.2002	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Chapman SC, Schubert FR, Schoenwolf GC, Lumsden A. Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos. Dev Biol. 2002 May 1;245(1):187-99.
Copyright	2002 Elsevier Science (USA). All rights reserved.

Stage

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Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XIV

Hypoblast
Kohler's Sickle

1-3

stage 3

Endoderm, early
Hypoblast
Primitive Streak

4-6

stage 4

stage 5

stage 6

Blood Islands
Endoderm
Germinal Crescent
Head Process
Hensen's Node
Primitive Streak

Chordin expression in the notochord shown in red.
Prechordal Plate

Entry 5

GEISHA Id	HEX.Ishii.2007	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Ishii Y, Langberg JD, Hurtado R, Lee S, Mikawa T. Induction of proepicardial marker gene expression by the liver bud. Development. 2007 Oct;134(20):3627-37. Epub 2007 Sep 12.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. Permission granted by T. Mikawa.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205252).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 11

stage 12

Liver

13-16

stage 14

stage 16

Liver

Entry 6

GEISHA Id	Hex.Klewer.2006	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Klewer SE, Yatskievych T, Pogreba K, Stevens MV, Antin PB, Camenisch TD. Has2 expression in heart forming regions is independent of BMP signaling. Gene Expr Patterns. 2006 Jun;6(5):462-70. Epub 2006 Feb 3.
Copyright	Reprinted with permission from Elsevier. Copyright © 2005 Elsevier B.V. All rights reserved.
Comments	According to authors , "... Hex (full-length, a the gift of G.H. Goodwin, Institute of Cancer Research, London (Zhang et al., 2004)). " As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205252)

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 7

Blood Islands
Lateral Plate Mesoderm

Cardiac crescent

Entry 7

GEISHA Id	Hex.Chapman.2008	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Comments	Images Provided by Susan Chapman. Insufficient information provided to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #NM_205252).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XII

stage XIII

Hypoblast
Kohler's Sickle

1-3

stage 2

stage 3

Early Endoderm
Germinal Crescent
Head Process
Headfold
Hensen's Node
Hypoblast
Primitive Streak

Chd:red
Double labeled
Hex:blue

4-6

stage 4

stage 5

Anterior Intestinal Portal
Blood Islands
Early Endoderm
Epiblast
Germinal Crescent
Head Process
Hensen's Node
Hypoblast
Liver

Chd:red
Double labeled
Ganf:red
Hex:blue

7-12

stage 8

stage 9

stage 11

stage 12

Anterior Intestinal Portal
Blood Islands
Blood Vessels
Early Endoderm
Head Mesenchyme
Heart Tube
Liver

Liver precursor endoderm

13-14

stage 14

Blood Islands
Hindgut
Liver
Proepicardium
Thyroid

Entry 8

GEISHA Id	HEX.Bertocchini.2008	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Federica Bertocchini, Claudio D Stern (2008). A differential screen for genes expressed in the extraembryonic endodermal layer of pre-primitive streak stage chick embryos reveals expression of Apolipoprotein A1 in hypoblast, endoblast and endoderm. Gene Expression Patterns, Volume 8, Issues 7-8, September 2008, Pages 477-480, ISSN 1567-133X, DOI: 10.1016/j.gep.2008.07.001.
Copyright	Copyright © 2008 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205252).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XIV

Hypoblast

Stage XIV EG&K

Entry 9

GEISHA Id	HEX.Foley.2000	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Foley AC, Skromne I, Stern CD. Reconciling different models of forebrain induction and patterning: a dual role for the hypoblast. Development. 2000 Sep;127(17):3839-54.
Copyright	Printed in Great Britain © The Company of Biologists Limited 2000
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205252).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XIII

Hypoblast

Stage XII-XIII

1-3

stage 2

Hypoblast

stage 4

Hypoblast
Primitive Streak

Entry 10

GEISHA Id	HEX.Lopez-Sanchez.2009	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Lopez-Sanchez C, Garcia-Masa N, Gañan CM, Garcia-Martinez V. Movement and commitment of primitive streak precardiac cells during cardiogenesis. Int J Dev Biol. 2009;53(8-10):1445-55.
Copyright	Reprinted from Int. J. Dev. Biol. Copyright (2009), with permission from UBC Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #NM_205252.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Early Endoderm
Early Mesoderm

gene was expressed in the lateral endoderm and bilateral mesoderm
gene was expressed in the lateral endoderm and bilateral mesoderm.

7-8

stage 7

stage 8

Early Endoderm
Liver

gene expressed in the level of splanchnic mesoderm of the heart rudiment
gene was expressed in the pericardial splanchnic mesoderm

Entry 11

GEISHA Id	HHEX.Yaklichkin.2011	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Yaklichkin SY, Darnell DK, Pier MV, Antin PB, Hannenhalli S. Accelerated evolution of 3'avian FOXE1 genes, and thyroid and feather specific expression of chicken FoxE1. BMC Evol Biol. 2011 Oct 15;11:302.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205252.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-17

stage 14

stage 17

Thyroid

Entry 12

GEISHA Id	HHEX.Lee.2020	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Hyung Chul Lee, Hui-Chun Lu, Mark Turmaine, Nidia M. M. Oliveira, Youwen Yang† , Irene De Almeida and Claudio D. Stern. Molecular anatomy of the pre-primitive streak chick embryo Open Biol. null. 10.1098/rsob.190299.
Copyright	Copyright © 2020 The Authors.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205252.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage X

stage XII

stage XIV

Hypoblast
Unlabeled

Stage EGK X-XI
Stage EGK XII-XIII
Stage EGK XIII-XIV

all

Entry 13

GEISHA Id	cHEX.Yatskievych.1999	Data Source	Publication
Complete cDNA Template Probe	show GCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGGGGCCGGGGAG CCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTACGCGCCCACG CCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCCGCGCCGGCCC CCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGGCCCCGTACCG GACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCACCGGCGCTTAC GCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCCCTGGGAAAGC CGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCAACGAGCAGAC CATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAAGCTGCTGCAG CTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAGAACCCCCAGG CCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGTCGGAGGGCAGCCCCAGCCCCGCGGGGGG GGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGACGACTCCGAC CAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAGCCGAGCTCGG CTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAAAGACAAAGAA GCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTTTTTTGGCAGA TCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTATGTTGATTGGTGCTCTG ACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGGGGAGCACTGAG GGCACCACCGTGCCTTTGTGTGTGCGAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCAGGCAGAGAAAT GTATTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCTGCATCCCCCGG AAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTCCCTCATCGCAT TTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAAAACAAAACAAAAACAAAAACCAAAC ACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAGAAGG AAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGACAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCTCGTG TACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAAAAGA AAAAAAAAAAAAGGAATCAAAAACTGGT
Citation	Yatskievych TA, Pascoe S, Antin PB. Expression of the homebox gene Hex during early stages of chick embryo development. Mech Dev. 1999 Jan;80(1):107-9
Copyright	© 1999 Elsevier Science Ireland Ltd All rights reserved
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205252.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XII

Hypoblast
Kohler's Sickle

4-6

stage 5

stage 6

Area Opaca
Early Endoderm
Extraembryonic Mesoderm
Head Process
Hensen's Node
Hypoblast

head fold
stage 5 -

7-12

stage 9

stage 10

stage 12

Anterior Intestinal Portal
Aorta
Blood Islands
Blood Vessels
Central Nervous System
Endocardium
Endoderm
Endothelial Cells
Intermediate Mesoderm
Myocardium
Paraxial Mesoderm
Thyroid

cardiogenic crescent
dorsal aorta
endoderm adjacent to the vitelline vein
intersomtic vessels

13-14

stage 14

Blood Islands
Intermediate Mesoderm
Liver
Renal
Tail
Thyroid

all

Entry 14

GEISHA Id	HHEX.Anderson.2021	Data Source	Direct Submission
Complete cDNA Template Probe	show GGAGAAACCCGCGGCGGCGGTGGCAGCAGCCGGCGGCCGCGCTCGGGGCCGCTCTCCGAGGGGCGGCCGGGCCGCGGGG GGCCGGGGAGCCGCGCCATGCAGTATCAGGCGCCGGGCGCGGCTCCGGCGGCGGCCCTGGGCGTCGGCGTCCCTCTGTA CGCGCCCACGCCGCTGCTGCAGCCCGCGCACCCCACGCCCTTCTACATCGAGGACATCCTGGGCCGCGGCCCCGCCGCC GCGCCGGCCCCCCACTCCCTGCCCGCCCCGCCGCCGCCGACGCTGCCGTCGCCCAACTCCTCCTTCACCAGCCTGGTGG CCCCGTACCGGACCCCCGTCTACGAGCCGACCCCCATCCACCCGGCCTTCTCTCACCACCTCGCCGCCACCTACGGCAC CGGCGCTTACGCCGGGCCCCTCTACTCCTTTCCCCGCGCCGTCGGCGACTACACGCACGCACTGATCCGCCAGGACCCC CTGGGAAAGCCGCTGCTGTGGAGCCCCTTCATCCAGCGGCCGCTGCATAAGAGGAAGGGTGGGCAGGTGCGCTTCTCCA ACGAGCAGACCATCGAGCTGGAGAAGAAGTTCGAGACGCAGAAATACCTCTCCCCGCCCGAGAGGAAGCGCCTGGCCAA GCTGCTGCAGCTCAGCGAGCGCCAGGTCAAAACGTGGTTCCAGAACCGCAGAGCCAAATGGAGGCGACTGAAGCAGGAG AACCCCCAGGCCACCAAAAAGGAGGAGGCGGAAGGCACCGGCGATCACGGCGACCCTCGGCCGGAGGGCAGCCCCAGCC CCGCGGGGGGGGGCGAGGCCGAGCCGCAGGACAGCCCTTCGGCCGCCTCGCAGGAGGACCCCGAGTCCGACGTCTCCGA CGACTCCGACCAGGAGGTGGACATCGAGGGCGACAAAGGCTTCTACAGCGCCACACGCTGACGGCCCGCGGGCACGGAG CCGAGCTCGGCTTGCGCTGTCCCGGGACTGCGGGAAGGAGGATTTTATGACGGGAGAGGACCTTCACCATTCCGCCGAA AGACAAAGAAGCACGAACGGACTCGTTCTGTAATAATCTGAGAAAGAAAAGATATTCGTTCTGCAAACGGTCCGTTTTT TTTTTGGCAGATCTCTGATTTGATTGTAGGGACTGATCGGTGGTTTGTATTTACTGTGTAAGATCTGTGTGTACGTTGA TTGGTGCTCTGACCGGGACACAGCGCTGTGCCCATCTCCGGCTGCTGGAACCCCCCTCTTCCCCAGCTCGTGTAGTGGG GGAGCACTGAGGGCAGGACCGTGCCTTTGTGTGTGCAAACGAATCTGCTTTAAATGGAGTAATTTAAGTGTCCTTCGCA GGCAGAGAAGTGTTTTCTGTGCCATAGAGAGGGATTGGAGCATCCCGAGAGTGCCTTACCCCACCCAGGACCTGCAGCT GCATCCCCCGGAAGGATCCCCCCTCACCGGGCAGAGGCTGTGGGCACGTTCCGTTTTGCTCTTGATGGAGTGAAATGTC CCTCATCGCATTTTGGAGGATTTTGCCCCACAATCTGTGTGGTTCCATTTGCAGGGAAAAAAAAACAAAAACCAAAACC AAACACAGCAACAAACCAACCCAGGCTATTTTTTTCCTTGACAAATCTGTCGTGCTGTCCCCCAGATTTCCCATTTCAG AAGGAAATGAGGGGCTGATAGGGCACTGCGAGGCGCAGTGTGGCAGCGGGCACCCGTGCCTGGCGTGTCCCTCTCTGCT CGTGTACATAGCAGTTTGCCTTAAAAGTCTTTGCGGTTTTATACCTCACATTGTTCATATTTTGTACATATTGGTTTAA AAGAAAAAAAAAAAGGAATCAAAAACTGGTATTTAATTTCTGATGGATTTCTTCGAAATAAAATGAAACTCTGCACCA
Comments	Images provided by Claire Anderson. As insufficient information was provided to verify sequence used to synthesize probe, the sequence above was obtained from NCBI (accession number NM_205252.2). Reference paper: T.A. Yatskievych, S. Pascoe, P.B. Antin. Expression of the homebox gene Hex during early stages of chick embryo development. Mech. Dev., 80 (1999), pp. 107-109.