The following in situ probes were used: Wnt1, Wnt3a, Wnt4 (Hollyday et al., 1995), who indicated: cDNA was amplified using 200 ng of each
of the following degenerate oligonucleotide primers [5-
ATAAGATCTGGTGGGG(A/G/C/T)AT(A/C/T)GT- 3] (encompassing WWGIV in Wnt-1) and [5-TATGGATCCCCA(A/G)TGCCA(A/G)CT-3] (encompassing DWHWG in Wnt-1).
According to the authors, "... Wnt-1 (Hollyday et al., 1995), were used as previously described." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (AY655699).