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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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PCDH8
protocadherin 8

Gene

Official Symbol	PCDH8
Official Full Name	protocadherin 8
CGNC ID	53254
also known as	protocadherin-8, protocadherin-8\|paraxial protocadherin 8 short isoform
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_001098609
Polypeptide	NP_001092079
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.30329

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	5100	ENSG00000136099		MIM:603580
Mouse	18530	ENSMUSG00000036422	All phenotypic alleles (1):Targeted, knock-out(1)	MGI:1306800
Xenopus	448128, 379380			5887885
Zebrafish	100002026, 30456	ENSDARG00000006467	ZDB-GENE-980526-242	ZFIN:ZDB-GENE-980526-242

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	428065
Ensembl Gene
KEGG	gga:428065

Entry 1

GEISHA Id	Procad8	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show ACGGTGTGTGGAGTTGAGTGCAGTCCCCGCACCGCGTCCCCTCTCTGAGTCCCGCGCAGGTGCCATGAGCCCCCTGCGG CTGCTGGCCGCCGGCTGCCTGCTGGCCCTGTCCCACTGCAAGACGGTGCGCTACCGCACCGACGAGGAAGGCGCGCCGG GCACGGTGATCGGCACGCTGGCCGACGAGATGCCGGTGAAGGCTCCGGGGGAGATGAGCTTCCGACTGATGCGGCAGTT CAGCAACAGCTCGCTGGTGCGGGTGCGGGAGGAGGACGGGCAGCTGAGCATCGGCGAGGCTGGGCTGGACCGGGAGCGG CTGTGCGGGCAGGCCCCGCAGTGCGTGCTGGCCTTCGACGTGGTGAGCTGCTGGCGGGAGCGCTACCGCCTGGTGCACG TGGAGCTGGAGGTGCGAGACATCAACGACAACGCGCCCCACTTCCCCCGTGCCCAGATGGCGCTGGAGGTGTCGGAGAG CGCCGCTCCTGGCACTCGCCTCCCACTCGAGGTGGCCGTGGACGAGGATGTGGGCTCCAACTCCATCCAGAGCTTCCAG GTCTCCCTCAACAGCCACTTCGGTGTGGAAGCGCAGACGCGGGCAGACGGGGCGCGCTGCGCGGATCTGGTGCTGCTGC AGGAGCTGGACCGCGAGCGCCAGCCTTCCTACACCCTGGAGCTGGTGGCCAAGGACGGCGGCAGCCCTGCACGCTCAGG CACAGCCACGGTGCACGTCCGCGTCCTCGATGCCAACGACAACAGCCCCACTTTTGCCCGGAGCTCTGTGACAGTGGAG CTGCCGGAAGACGCGCCGCCTGGCTCCCTGCTGCTTGACCTGGATGCCGAAGACCCCGACGAGGGCCCCAACGGAGAGG TCGTCTATGCCTTTGGCAGTCAGGTGCCCCCTGAGGCACGGCGGCTCTTCCGCCTAGACCCACGCTCAGGCCGCCTGAC GCTGGAGGCTGCCGTGGACTATGAGCGTACCCGCACCTACGAGCTGGACGTGCGGGCCCAGGATCGCGGTGCCAGCCCC AGGACCGCGACCTGCACCGTCGTGGTGCGCCTTACCGACGTCAACGACAACGCGCCACGCATCAGCATCAGCGCCCTGC GTGGTGCCGGCAGCGCCGCCGGCGTGGCCTACGTCAGCGAGGCGGCTGCCAGTGAGAGCTTCGTGGCCCTCGTCAGCGC TTCCGACCGTGACTCTGGCACCAACGGGCAGGTGCGCTGCAGCCTGCGAGGCCACGACCACTTTGCCCTGCAGCGGGCC TACGAGGACAGCTACATGATCGTCACCACGGCGGCGCTGGATCGCGAGCGCATCCCCGAGTACAATCTCACCGTGGTGG CCGAGGACCTGGGCTCCCCGCCCTTCAAGACCGTCCGCCAGTACACGGTGCGGGTGAGCGACGAGAACGACAACGCGCC GCTCTTTGCCAAGCCCCTCTATGAGGTGGCTGTGCCGGAGAACAACCCGCCAGGTGCCTACATCACCACTGTGGTGGCC CGTGACCCTGATCTTGGCCACAATGGGAAGGTCACCTACCGGCTGCTGGAGACCCAAGTCATGGGGGCCCCAATCTCTA CCTACGTCTCGGTTGACCCTGCCACTGGGGCCATCTATGCCCTCAGAACGTTCAACTATGAGATCCTCAAGCAGCTTGA CCTGAGGATCCAGGCCACTGATGGAGGCTCCCCGCAGCTCTCCAGCAGCACGCTTGTCAAGGTGAGGATGGTGGACCAG AATGACAACCCTCCAATCATCATCCACCCAGTGCTCACCAATGGGTCGGTGGAAATCAGTGTCTCCAGCAAGACCTCCC GTGATGCCTTGGTGGCCCAGATCAAAGCCCGCGATGCAGATGATGGGGCCAACGCTGAGCTTACCTTTGCCTTCCTGGA AGAACCCCAGCAGGACCTTTTTGCCATCAACCCAAGCACTGGAGACATCGTGCTGAGGGGTGACCTCTCTGAAGAGCTG GGGCAGCTGTTCAAGGTCATCCTCACTGTGACAGATAATGGGAGGCCCCCTCTGGCCACAACTGCCACTGTTAACTTCC TGGTGACCGCCACTGCTCCTTCCAGCATCCAGGAGGTAGCCAAGCCCAGCTCCTGGCAAGGAAAGGCCTTGCAGTGGGA CATCCCTCTGATTGTGATCATCGTCCTGGCGGGCAGCTGCACCCTCCTCCTGGTGGCCATCATCACCATCGCCACCACC TGCAACAGGCGCAAGAAAGGGAACGAGATCAAAAACAACAGTCCCCTGAAGGAGCAGATCGACATTTCCCACCTGGAGA AGGGTCGGCAGGAGGACAGCAGCCAGAGGGGAAACGTGTTCGAGGTACGAACCTTCCCCAGCAAAACCCCTTTCAGCGG CCCCGACCCCCCTTCAGCAGCTGAGGAGATCTCCACCGCGGAGAGCAGCAGCGACAGCGCCTGCCTCTACGACGGTCAG AAGCGGCTCAGAGGAGCCGGCGGGGAGGGTTTCACCGCCGCTCCGAGCTTCGGCAAAGAGCCCGCTCCCCCCGTGGCCA TTTGGAAGGGGCACTCCTTTAATACCATCTCGGGCCGGGAGGCAGAGAAGTTCAGCGGCAAGGACAGTGGCAAAGGCGA CAGCGATTTCAACGACAGCGACTCGGACATCAGCGGGGATGCCCTGAAGAAGGACCTCATCACACACATGCAGAACGGT CTGTGGGCTTGTACGGCCGAGTGCAAGATCCTGGGCCATTCGGACCGCTGCTGGAGCCCCTCCTGTGGCCGAGCCAACC CGCATCCCTCTCCCCACCCTCCGGCGCCTCTCTCCACCTTCTGCAAAAGCACATCCTTGCCCCGGGACCCTCTCCGCAG GGACAACTTTTACCAGGCCCAGCTGCCAAAGACAGTGGGGCTGCAGAGCGTCTATGAGAAGGTGTTGCACAGGGACTTT GACCGGACGATCACGCTGCTGTCCCCTCCGCGTCCCGCTCGGCTCCCTGACCTCCAGGAGATTGGGGTGCCCCTCTACC CGGCCCCCTCAACCAGATTCCTTGGCCCCCAGACTGATGCCACTGAGAAAGCATAACCTCCCCACCCCTGGCCCCATCC GCACGACTCAGTTGCTCCTGGGAGCCTTTAAGTTATTGACCAGACCCGGTGTTGTACGTGTAAATATGCAAGATGTGCC TTAAAACATGAAGGTGTTGGGAAAGATTTCCCGTCGGTACTGTTTGGTATTTGCAAACAACAGCAACAACAAAAATTGT TTGTAGTTCATGTAATTTTTATGAAATGTGCAGTATTTAATTTTTTTTTTCCCCCTTTGTTTTCTACATGGTTTTCTTT GCGTTTGGTTTGCCCATGGCCTGCCATTTTTGTAGTGTTTTTAACCTGTACAGTGTGTAATGTAATGTTTGTACATAAT GAGAATTGGTTATATTTTTATATAATAAAAGCTTAAATAATGGGAATTCTTGCCAAAGGAACTGTCTGAGAGACACAAT AATTATTATTAATATCCTGCATATGTAGAATCTTGTAAGGGAAATTCCTCTTCCGTAGTGTAATAGTAACCCAAGCAAC ACAGTGTGCGGAGACGTTTGCCTTGCCAAATGTGATTTGTATCTCTTGAATCTGTAGTCGGTTTAAAGTTAAATGTTAT TTAATTGCCTTTGGTTCCTGTAATCTGTGTCACTGATAAACACTAATAAAGGTTTTGTGATGTGTTGG
Comments	probe not sequenced yet, this clone is supposed to be the NCBI long form: acc # XM_425623

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Paraxial Mesoderm
Trunk Mesenchyme
Unlabeled

7-12

stage 7

stage 8

stage 9

stage 11

Lateral Plate Mesoderm
Neural Crest
Paraxial Mesoderm
Somites

13-14

stage 13

stage 14

Heart
Heart Tube
Hindbrain
Paraxial Mesoderm
Somites
Spinal Cord

Entry 2

GEISHA Id	PCDH8.Watanabe.2009	Data Source	Publication
Complete cDNA Template Probe	show `CAGCGGCAAGGACAGTGGCAAAGGCGACAGCGATTTCAACGACAGCGACTCGGACATCAGCGGGGATGCCCTGAAGAAG GACCTCATCACACACATGCAGAACGGTCTGTGGGCTTGTACGGCCGAGTGCAAGATCCTGGGCCATTCGGACCGCTGCT GGAGCCCCTCCTGTGGCCGAGCCAACCCGCATCCCTCTCCCCACCCTCCGGCGCCTCTCTCCACCTTCTGCAAAAGCAC ATCCTTGCCCCGGGACCCTCTCCGCAGGGACAACTTTTACCAGGCCCAGCTGCCAAAGACAGTGGGGCTGCAGAGCGTC TATGAGAAGGTGTTGCACAGGGACTTTGACCGGACGATCACGCTGCTGTCCCCTCCGCGTCCCGCTCGGCTCCCTGACC TCCAGGAGATTGGGGTGCCCCTCTACCCGGCCCCCTCAACCAGATTCCTTGG`
Citation	Watanabe T, Sato Y, Saito D, Tadokoro R, Takahashi Y. EphrinB2 coordinates the formation of a morphological boundary and cell epithelialization during somite segmentation. Proc Natl Acad Sci U S A. 2009 May 5; 106(18): 74677472.
Copyright	Copyright 2009 national Academy of Sciences USA
Comments	The probe was amplified by PCR using the following primers: 5′-GACAGCGGCAAGGAGACAGTGATTTCAATGACAGTGACTCGG-3′ and 5′-CCAAGGAATGTGGTTGAGGGGCCGGGTAGAGGGGCACCCC-3′ and was extracted from NCBI Acc# NM_001098609.1.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Somites

Entry 3

GEISHA Id	PCDH8.Lin.2012	Data Source	Publication
Complete cDNA Template Probe	show TCGCGAGCGCCAGCCTTCCTACACCCTGGAGCTGGTGGCCAAGGACGGCGGCAGCCCTGCACGCTCAGGCACAGCCACG GTGCACGTCCGCGTCCTCGATGCCAACGACAACAGCCCCACTTTTGCCCGGAGCTCTGTGACAGTGGAGCTGCCGGAAG ACGCGCCGCCTGGCTCCCTGCTGCTTGACCTGGATGCCGAAGACCCCGACGAGGGCCCCAACGGAGAGGTCGTCTATGC CTTTGGCAGTCAGGTGCCCCCTGAGGCACGGCGGCTCTTCCGCCTAGACCCACGCTCAGGCCGCCTGACGCTGGAGGCT GCTGTGGACTACGAGCGTACCCGCACCTACGAGCTGGACGTGCGGGCCCAGGATCGCGGTGCCAGCCCCAGGACCGCGA CCTGCACCGTCGTGGTGCGCCTTACCGACATCAACGACAACGCGCCACGCATCAGCATCAGCGCCCTGCGTGGTGCCGG CAGCGCCGCCGGCGTGGCCTACGTCAGCGAGGCGGCTGCCAGTGAGAGCTTCGTGGCCCTCGTCAGCGCTTCCGACCGT GACTCTGGCACCAACGGGCAGGTGCGCTGCAGCCTGCGAGGCCACGACCACTTTGCCCTGCAGCGGGCCTACGAGGACA GCTACATGATCGTCACCACGGCGGCGCTGGATCGCGAGCGCATCCCCGAGTACAATCTCACCGTGGTGGCCGAGGACCT GGGCTCCCCGCCCTTCAAGACCGTCCGCCAGTACACGGTGCGGGTGAGCGACGAGAACGACAACGCGCCGCTCTTTGCC AAGCCCCTCTATGAGGTGGCTGTGCCGGAGAACAACCCGCCAGGTGCCTACATCACCACTGTGGTGGCCCGTGACCCTG ATCTTGGCCACAATGGGAAGGTCACCTACCGGCTGCTGGAGACCCAAGTCATGGGGGCCCCAATCTCTACCTACGTCTC GGTTGACCCTGCCACTGGGGCCATCTATGCCCTCAGAACGTTCAACTATGAGATCCTCAAGCAGCTTGACCTGAGGATC CAGGCCACTGATGGGGGCTCCCCGCAGCTCTCCAGCAGCACGCTTGTCAAGGTGAGGATGGTGGACCAGAATGACAACC CTCCAATCATCATCCACCCAGTGCTCACCAATGGGTCGGTGGAAATCAGTGTCTCCAGCAAGACCTCCCGTGATGCCTT GGTGGCCCAGATCAAAGCCCGCGATGCAGATGATGGGGCCAACGCTGAGCTTACCTTTGCCTTCCTGGAAGAACCCCAG CAGGACCTTTTTGCCATCAACCCAAGCACTGGAGACATCGTGCTGAGGGGTGACCTCTCTGAAGAGCTGGGGCAGCTGT TCAAGGTCATCCTCACTGTGACAGATAATGGGAGGCCCCCTCTGGCCACAACTGCCACTGTTAACTTCCTGGTGACCGC CACTGCTCCTTCCAGCATCCAGGAGGTAGCCAAGCCCAGCTCCTGGCAAGGAAAGGCCTTGCAGTGGGACATCCCTCTG ATTGTGATCATTGTCCTGGCGGGCAGCTGCACCCTCCTCCTGGTGGCCATCATCACCATCGCCACCACCTGCAACAGGC GCAAGAAAGGGAACGAGATCAAAAACAACAGTCCCCTGAAGGAGCAGATCGACATTTCCCACCTGGAGAAGGGTCGGCA GGAGGACAGCAGCCAGAGGGGAAACGTGTTCGAGGTACGAACCTTCCCCAGCAAAACCCCTTTCAGCGGCCCCGACCCC CCTTCAGCAGCTGAGGAGATCTCCACCGCGGAGAGCAGCAGCGACAGCGCCTGCCTCTACGACGGTCAGAAGCGGCTCA GAGGAGCCAGCGGGGAGCAGG
Citation	Lin J, Wang C, Redies C Expression of delta-protocadherins in the spinal cord of the chicken embryo. J Comp Neurol. 2012 May 1;520(7):1509-31.
Copyright	Copyright © 2011 Wiley Periodicals, Inc.
Comments	The cDNA template used to generate the probe was obtained by PCR as described in the paper.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Spinal Cord

19-24

stage 20

stage 24

Notochord
Spinal Cord
Spinal Ganglia and Nerves

Cdh7: green
Higher magnification of boxed area in C.
Pax3: red
Pcdh10: green
Pcdh18: red
Pcdh7: red
Pcdh8: green
Pcdh8: red
ncc, neural crest cells

25-30

stage 29

stage 30

Spinal Cord
Spinal Ganglia and Nerves

Cervical section of the spine.
Higher magnification of boxed are in E.
Higher magnification of boxed area in G.
Immunostaining: Islet1 in red
In situ hybridization: PC8 in green
Lumbar section of the spine.
PC8: green fluorescence
Sacral section of the spine.
Thoracic section of the spine.
dh, dorsal horn

31-36

stage 34

stage 36

Spinal Cord
Spinal Ganglia and Nerves

Higher magnification of boxed area in I.
Higher magnification of the boxed area in K.
lm, lateral motor column
me, meninges
rp, roof plate

Entry 4

GEISHA Id	PCDH8.Lin.2013	Data Source	Publication
Complete cDNA Template Probe	show TCGCGAGCGCCAGCCTTCCTACACCCTGGAGCTGGTGGCCAAGGACGGCGGCAGCCCTGCACGCTCAGGCACAGCCACG GTGCACGTCCGCGTCCTCGATGCCAACGACAACAGCCCCACTTTTGCCCGGAGCTCTGTGACAGTGGAGCTGCCGGAAG ACGCGCCGCCTGGCTCCCTGCTGCTTGACCTGGATGCCGAAGACCCCGACGAGGGCCCCAACGGAGAGGTCGTCTATGC CTTTGGCAGTCAGGTGCCCCCTGAGGCACGGCGGCTCTTCCGCCTAGACCCACGCTCAGGCCGCCTGACGCTGGAGGCT GCTGTGGACTACGAGCGTACCCGCACCTACGAGCTGGACGTGCGGGCCCAGGATCGCGGTGCCAGCCCCAGGACCGCGA CCTGCACCGTCGTGGTGCGCCTTACCGACATCAACGACAACGCGCCACGCATCAGCATCAGCGCCCTGCGTGGTGCCGG CAGCGCCGCCGGCGTGGCCTACGTCAGCGAGGCGGCTGCCAGTGAGAGCTTCGTGGCCCTCGTCAGCGCTTCCGACCGT GACTCTGGCACCAACGGGCAGGTGCGCTGCAGCCTGCGAGGCCACGACCACTTTGCCCTGCAGCGGGCCTACGAGGACA GCTACATGATCGTCACCACGGCGGCGCTGGATCGCGAGCGCATCCCCGAGTACAATCTCACCGTGGTGGCCGAGGACCT GGGCTCCCCGCCCTTCAAGACCGTCCGCCAGTACACGGTGCGGGTGAGCGACGAGAACGACAACGCGCCGCTCTTTGCC AAGCCCCTCTATGAGGTGGCTGTGCCGGAGAACAACCCGCCAGGTGCCTACATCACCACTGTGGTGGCCCGTGACCCTG ATCTTGGCCACAATGGGAAGGTCACCTACCGGCTGCTGGAGACCCAAGTCATGGGGGCCCCAATCTCTACCTACGTCTC GGTTGACCCTGCCACTGGGGCCATCTATGCCCTCAGAACGTTCAACTATGAGATCCTCAAGCAGCTTGACCTGAGGATC CAGGCCACTGATGGGGGCTCCCCGCAGCTCTCCAGCAGCACGCTTGTCAAGGTGAGGATGGTGGACCAGAATGACAACC CTCCAATCATCATCCACCCAGTGCTCACCAATGGGTCGGTGGAAATCAGTGTCTCCAGCAAGACCTCCCGTGATGCCTT GGTGGCCCAGATCAAAGCCCGCGATGCAGATGATGGGGCCAACGCTGAGCTTACCTTTGCCTTCCTGGAAGAACCCCAG CAGGACCTTTTTGCCATCAACCCAAGCACTGGAGACATCGTGCTGAGGGGTGACCTCTCTGAAGAGCTGGGGCAGCTGT TCAAGGTCATCCTCACTGTGACAGATAATGGGAGGCCCCCTCTGGCCACAACTGCCACTGTTAACTTCCTGGTGACCGC CACTGCTCCTTCCAGCATCCAGGAGGTAGCCAAGCCCAGCTCCTGGCAAGGAAAGGCCTTGCAGTGGGACATCCCTCTG ATTGTGATCATTGTCCTGGCGGGCAGCTGCACCCTCCTCCTGGTGGCCATCATCACCATCGCCACCACCTGCAACAGGC GCAAGAAAGGGAACGAGATCAAAAACAACAGTCCCCTGAAGGAGCAGATCGACATTTCCCACCTGGAGAAGGGTCGGCA GGAGGACAGCAGCCAGAGGGGAAACGTGTTCGAGGTACGAACCTTCCCCAGCAAAACCCCTTTCAGCGGCCCCGACCCC CCTTCAGCAGCTGAGGAGATCTCCACCGCGGAGAGCAGCAGCGACAGCGCCTGCCTCTACGACGGTCAGAAGCGGCTCA GAGGAGCCAGCGGGGAGCAGG
Citation	Lin J, Wang C, Redies C. Expression of multiple delta-protocadherins during feather bud formation. Gene Expr Patterns. 2013 Mar-Apr;13(3-4):57-65. Epub 2013 Jan 11.. DIO: 10.1016/j.gep.2013.01.001.
Copyright	Copyright © 2013 Elsevier B.V. All rights reserved.
Comments	The complete cDNA template sequence was obtained from the information from the publication as described in Lin et al. 2013.