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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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TNNI3
troponin I type 3 (cardiac)

Gene

Official Symbol	TNNI3
Official Full Name	troponin I type 3 (cardiac)
CGNC ID	49798
also known as	troponin I, cardiac muscle\|cardiac troponin I
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_213570
Polypeptide	NP_998735
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.820

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function	actin binding, metal ion binding
Biological Process
Cellular Component	troponin complex

Links to other databases

Entrez Gene	396428
Ensembl Gene
KEGG	gga:396428

Entry 1

GEISHA Id	CTNI.vandenBerg.2011	Data Source	Publication
Complete cDNA Template Probe	show CAGCACCGATGCACGATGGCTGAGGAAGAGGAGCCGAAGCCCCCCCCTCTGCGGCGCAAATCATCCGCCAACTACCGGG GTTACGCTGTGGAGCCCCACGCCAAGAGACAATCCAAGATCTCGGCTTCCCGCAAGCTGCAGCTCAAGACGTTGCTGCT GCAGAGAGCAAAGCGGGAGTTGGAGCGCGAGGAGCAGGAGAGGGCAGGAGAGAAACAGCGGCACCTGGGGGAGCTGTGC CCCCCCCCGGAGCTGGAGGGCTTGGGGGTGGCCCAGCTGCAGGAGCTGTGCCGGGAGCTGCACGCCCGCATAGGGCGCG TGGATGAGGAGCGCTACGACATGGGGACGCGCGTCAGCAAGAACATGGCCGAGATGGAGGAGCTGCGGCGCCGCGTGGC CGGGGGTCGCTTTGTTCGCCCCGCGCTGCGCCGCGTGCGGCTGTCGGCCGACGCCATGATGGCGGCGCTGCTGGGCTCC AAGCACCGCGTGGGCACCGACCTGAGGGCCGGGCTGCGGCAGGTGCGGAAGGACGACGCCGAGAAGGAGAGCCGCGAGG TCGGCGATTGGCGGAAGAACGTGGACGCGCTGAGCGGCATGGAGGGGCGCAAGAAGAAGTTCGAGGCGCCGGGGGGGGG GCAGGGCTGAGGACC
Citation	van den Berg G, Moorman AF. Development of the pulmonary vein and the systemic venous sinus: an interactive 3D overview. PLoS One. 2011;6(7):e22055. Epub 2011 Jul 11.
Copyright	The work is protected by copyright held by the authors under the terms of the creative commons public license, attribution 2.5.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc AY463242.1)

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Aorta
Atria
Outflow Tract
Sinus Venosus
Ventricles

Entry 2

GEISHA Id	cTNI.Houweling.2002	Data Source	Publication
Complete cDNA Template Probe	show AAGCTGCAGCTCAAGACGTTGCTGCTGCAGAGAGCAAAGCGGGAGTTGGAGCGCGAGGAGCAGGAGAGGGCAGGAGAGA AACAGCGGCACCTGGGGGGACTGTGCCCCCCCCCGGAGCTGGAGGGCTTGGGGGTGGCCCAGCTGCAGGAGCTGTGCCG GGAGCTGCACGCCCGCATAGCGGTGGATGAGGAGCGCTACGACATGGGGACGCGCGTCAGCAAGAACATGGCCGAGATG GAGGAGCTGCGGCGCCGCGTGGCCGGGGGTCGCTTTGTTCGCCCCGCGCTGCGCCGCGTGCGGCTGTCGGCCGACGCCA TGATGGCGGCGCTGCTGGGCTCCAAGCACCGCGTGGGGACCGACCTGAGGGCCGGGCTGCGGCAGGTGCGGAAGGACGA CGCCGAGAAGGAGAGCCGCGAGGTCGGCGATTGGCGGAAAAACGTGGACGCGCTGAGCGGCATGGAGGGGCGCAAGAAG AAGTTCGAGGCGCCGGGGGGGGGGCAGGGCTGAGGGTGAGGACCCCCCCCGGAGCCCCCCCACCCCTCTCCACAAACCC CCTCCACGGATCCCCCCACCAGGACCCTCACCATTCAGACCTTCACCTTTAAGGGCAGCGTTGTGGCATGCAGCACCTT TAATCTCCACTCCCCCCCCCCCAATAATTTACTCCCCTCAATAAATTACCCCTCCCCCCAAC
Citation	Houweling AC, Somi S, Van Den Hoff MJ, Moorman AF, Christoffels VM. Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken. Anat Rec. 2002 Feb 1;266(2):93-102.
Copyright	Copyright © 19992012 John Wiley & Sons, Inc. All Rights Reserved.
Comments	Authors indicated the probe sequence is associated with GenBank # M73703 ....

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 9

Heart
Myocardium
Splanchnic Mesoderm

cardiac mesoderm ( CM) , anterior intestional portal show as "aip" in photo
early tubular heart / cardiac mesoderm

19-24

stage 21

stage 24

Myocardium
Outflow Tract
Ventricles

atrioventricular canal, inner curvature
outflowtract ( oft) and inflow tract ( ift)

25-30

stage 28

stage 30

Atria
Heart
Myocardium
Ventricles

atrioventricular bundle indicated by arrow
epicardium designated by abbreviation " ec" in photo , " me" referes to mesenchyme
epicardium is designated with by "ep" in photo

Entry 3

GEISHA Id	CTNI.vandenBerg.2007	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CAGCACCGATGCACGATGGCTGAGGAAGAGGAGCCGAAGCCCCCCCCTCTGCGGCGCAAATCATCCGCCAACTACCGGG GTTACGCTGTGGAGCCCCACGCCAAGAGACAATCCAAGATCTCGGCTTCCCGCAAGCTGCAGCTCAAGACGTTGCTGCT GCAGAGAGCAAAGCGGGAGTTGGAGCGCGAGGAGCAGGAGAGGGCAGGAGAGAAACAGCGGCACCTGGGGGAGCTGTGC CCCCCCCCGGAGCTGGAGGGCTTGGGGGTGGCCCAGCTGCAGGAGCTGTGCCGGGAGCTGCACGCCCGCATAGGGCGCG TGGATGAGGAGCGCTACGACATGGGGACGCGCGTCAGCAAGAACATGGCCGAGATGGAGGAGCTGCGGCGCCGCGTGGC CGGGGGTCGCTTTGTTCGCCCCGCGCTGCGCCGCGTGCGGCTGTCGGCCGACGCCATGATGGCGGCGCTGCTGGGCTCC AAGCACCGCGTGGGCACCGACCTGAGGGCCGGGCTGCGGCAGGTGCGGAAGGACGACGCCGAGAAGGAGAGCCGCGAGG TCGGCGATTGGCGGAAGAACGTGGACGCGCTGAGCGGCATGGAGGGGCGCAAGAAGAAGTTCGAGGCGCCGGGGGGGGG GCAGGGCTGAGGACC
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI Acc# NM_213570.1.