Geisha

home

contact

A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

Quick Search

Data Class

Enter Text:

Gene Family QuickSearch

	Transcription Factors
	Growth Factors
	Receptors
	MicroRNAs

Human Disease Gene Search

	Downloads
	Protocols
	About GEISHA
	Contact Us

	Transgenic Birds
	Model Organism Databases
	Gene Expression Databases
	Genomic Resources
	Anatomical Atlases
	Chicken Stage Series

FLRT3
fibronectin leucine rich transmembrane protein 3

Gene

Official Symbol	FLRT3
Official Full Name	fibronectin leucine rich transmembrane protein 3
CGNC ID	6617
also known as	leucine-rich repeat transmembrane protein FLRT3\|fibronectin-like domain-containing leucine-rich transmembrane protein 3
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	XM_426107
Polypeptide	XP_426107
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.50441

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	23767	ENSG00000125848		MIM:604808
Mouse	71436	ENSMUSG00000051379	All phenotypic alleles (6):Targeted, knock-out(4) Targeted, other(2)	MGI:1918686
Xenopus	100144692, 503672			490900
Zebrafish	558864	ENSDARG00000076895	ZDB-GENE-030131-1988	ZFIN:ZDB-GENE-030131-1988

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	428552
Ensembl Gene
KEGG	gga:428552

Entry 1

GEISHA Id	I38	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show GATCTATCTCAGACTTCTTTTTCTCTCTCGAAATCACCTTAGCACCATTCCCTGGGGTTTGCCTAAAACAATAGAAGAG CTACGCTTGGATGATAATCGTATTTCCACGATTTCAGAGCTGTCCCTTCAAGACCTTACAAATCTAAAACGCCTTGTTC TAGATGGAAATCTTCTAAATAACCACGGATTAGGAGACAAGGTCTTTATGAATCTAGTCAATCTTACAGAACTGTCATT GGTTCGCAATTCACTTACAGCCGCACCAGTAAATTTGCCAGGAACAAACCTAAGGAAGCTTTATCTGCAAGAAAACCAT ATCAATCACGTGCCACCCAATGCTTTCTCTTACTTAAGGCAGTTGTATCGACTAGATATGTCCAATAACAATCTCAGCA ATTTACCTCAGGGTGTCTTTGATGACCTGGACAACATAACTCAGCTGTTTCTTCGCAACAACCCTTGGCACTGTGGGTG CAAAATGAAGTGGGTCCGTGACTGGTTACAGTCATTGCCTTTGAAAGTGAACGTACGTGGGCTGATGTGCCAGGCGCCG GAAAAAGTGCGCGGAATGGCTATCAAAGACCTCAACGCGGAACTGTTCGATTGTAAGGATGACATGAGCACCATCCAGA TCACTACTGCGGTACCCAACACGCTGTACCCGGCCCAGGGACACTGGCCGGTTTCTGTGACCAAACAACCAGACATCAA GACCCCCCACCTAAACAAGAACTACAGAACCACGGCCAGCCCAGTACGCAAGATCATTACAATATTTGTGAAATCGGTG AGCACAGAGACCATCCACATCTCCTGGAAAGTTGCGCTNGCCAATGACAGCCTTACGGCTCAGCTGGCTCAAGATGGGC CACAGCCCTGCCTTTGGATCTATAACTGAAACCATCGTAACAGGGGACAGGAGTGACTACCTGCTTACAGCACTCGAAC CAGAGTCGCCATACCGCGTGTGCATGGTCCCCATGGAAACCAGCAACATCTATCTCTCTGACGAGACACCTGAATGCAT CGAGACCGAGACAGCTCCCCTTAAGATGTACAACCCGACAACCACACTCAACCGGGAGCAGGAAAAGGAGCCCTACAAG AACTCCAGCGTGCCTCTGGCCGCCATCATCGGTGGCGCGGTGGCACTGGTGGCCCTGGCACTGCTGGCCCTGGTGTGCT GGTACGTCCACAGGAATGGGGCCCTCTTCTCCCGGCACTGCGCCTACAGCAAGGGGCGCAGGAGGAAGGACGACTATGC TGAGGCGGGTACCAAGAAGGACAACTCCATCCTAGAAATCAGGGAGACTTCTTTTCAGATGATACCCATCACTAACGAC CAGGTGTCCAAGGAGGAGTTTGTAATACACACCATTTTCCCCCCTAATGGCATGAATCTGTATAAGAACAGCCACAGTG AAAGCAGTAGTAACAGGAGCTACAGAGACAGTGGTATTCCAGATTCAGATCATTCACACTCATGATACTGAAGGACATG AAAGACTGGTTTGGTTTTGGTTGGTTTGTTGTTTGGTTTTTTTT

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Unlabeled

4-6

stage 4

stage 6

Endoderm, early
Extraembryonic
Germinal Crescent
Hypoblast

7-12

stage 11

stage 12

Anterior Intestinal Portal
Endoderm
Heart
Heart Tube
Somites

13-18

stage 14

Ear/Otic Placcode
Somites
Surface Ectoderm

19-22

stage 22

Heart
Leg AER
Somites
Tail

Entry 2

GEISHA Id	Flrt3.Smith.2006	Data Source	Publication
Forward cDNA Template Probe	show AGATGAATTCCCCACTAACCTCCCTAAGTACGTCAAAGAACTGCATTTGCAGGAGAATAATATAAGGACCATCACTTAC GATTCACTTTCACAAATTCCTTATCTGGAAGAACTGCATTTGGATGATAATTCTGTTTCCGCTGTTAGCATTGAAGATG GAGCTTTCAGGGACAACATCTATCTCAGACTTCTTTTTCTCTCTCGAAATCACCTTAGCACCATTCCCTGGGGTTTGCC TAAAACAATAGAAGAGCTACGCTTGGATGATAATCGTATTTCCACGATTTCAGAGCTGTCCCTTCAAGACCTTACAAAT CTAAAACGCCTTGTTCTAGATGGAAATCTTCTAAATAACCACGGATTAGGAGACAAGGTCTTTATGAATCTAGTCAATC TTACAGAACTGTCATTGGTTCGCAATTCACTTACAGCCGCACCAGTAAATTTGCCAGGAACAAACCTAAGGAAGCTTTA TCTGCAAGAAAACCATATCAATCACGTGCCACCCAATGCTTTCTCTTACTTAAGGCAGTTGTATCGACTAGATATGTCC AATAACAATCTCAGCAATTTACCTCAGGGTGTCTTTGATGACCTGGACAACATAACTCAGCTGTTTCTTCGCAACAACC CTTGGCACTGTGGGTGCAAAATGAAGTGGGTCCGTGACTGGTTACAGTCATTGCCTTTGAAAGTGAACGTACGTGGGCT GATG
Reverse cDNA Template Probe	show ATGACATGAGCACCATCCAGATCACTACTGCGGTACCCAACACGCTGTACCCGGCCCAGGGACACTGGCCGGTTTCTGT GACCAAACAACCAGACATCAAGACCCCCAACCTAAACAAGAACTACAGAACCACGGCCAGCCCAGTACGCAAGATCATT ACAATATTTGTGAAATCGGTGAGCACAGAGACCATCCACATCTCCTGGAAAGTTGCGCTGCCAATGACAGCCTTACGGC TCAGCTGGCTCAAGATGGGCCACAGCCCTGCCTTTGGATCTATAACTGAAACCATCGTAACAGGGGACAGGAGTGACTA CCTGCTTACAGCACTCGAACCAGAGTCGCCATACCGCGTGTGCATGGTCCCCATGGAAACCAGCAACATCTATCTCTCT GACGAGACACCTGAATGCATCGAGACCGAGACAGCTCCCCTCAAGATGTACAACCCGACAACCACACTCAACCGGGAGC AGGAAAAGGAGCCCTACAAGAACTCCAGCGTGCCTCTGGCCGCCATCATCGGTGGCGCGGTGGCACTGGTGGCCCTGGC ACTGCTGGCCCTGGTGTGCTGGTACGTCCACAGGAATGGGGCCCTCTTCTCCCGGCACTGCGCCTACAGCAAGGGGCGC AGGAGGAAGGACGACTATGCTGAGGCGGGTACCAAGAAGGACAACTCCATCCTAGAAATCAGGGAGACTTCTTTTCAGA TGATACCCATCACTAACGACCAGGTGTCCAAGGAGGAGTTTGTAATACACACCATTTTCCCCCCTAATGGCATGAATCT GTATAAGAACAGCCACAGTGAAAGCAGTAGTAACAGGAGCTACAGAGACAGTGGTATTCC
Citation	Smith TG, Tickle C. The expression of Flrt3 during chick limb development. Int J Dev Biol. 2006;50(8):701-4.
Copyright	"Reprinted from Int. J. Dev. Biol., 50, Smith TG, Tickle C, The expression of Flrt3 during chick limb development, 701-4, Copyright (2006), with permission from Copyright © 2006 UBC Press". All rights reserved.
Comments	According to authors , "The chick Flrt3 orthologue was discovered from a search inthe ENSEMBL Gallus gallus genome browser: Identification ENSGALG00000008716. PCR was performed on stage HH16 whole-embryo cDNA to generate two probes that hybridized to different regions of the cFlrt3 gene. Primers used were 1) For: 5- AGATGAATTCCCCACTAACCTC -3 and Rev: 5- ACGTGCGGCCGCATCAGCCCACGTACGTTCAC -3. 2) For: 5- ACGTGAATTCATGACATGAGCACCATCCAG -3 Rev: 5- ACGTGCGGCCGCGGAATACCACTGTCTCTGTAG -3. Each PCR product was cloned into pBluescript (KS)." As information provided was insufficient to verify exact probe used for photos, the two probes are shown as 5' and 3' reads drawn from NCBI (acc # XM_426107).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 17

stage 18

Dermomyotome
Somatic Mesoderm
Wing AER
Wing Mesenchyme

19-24

stage 19

stage 20

stage 24

Dermatome
Isthmus/MHB
Lateral Plate Mesoderm
Leg Mesenchyme
Limb Buds
Midbrain
Nasal Placcode/Nerve
Retina
Somatic Mesoderm
Somites
Tail
Wing AER
Wing Mesenchyme

stage 25

Lateral Plate Mesoderm
Leg AER
Leg Mesenchyme
Paraxial Mesoderm
Wing AER
Wing Mesenchyme

Entry 3

GEISHA Id	FLRT3.Tomas.2011	Data Source	Publication
Complete cDNA Template Probe	show TCCAAGATCATTACAATATTTGTGAAATCGGTGAGCACAGAGACCATCCACATCTCCTGGAAAGTTGCGCTGCCAATGA CAGCCTTACGGCTCAGCTGGCTCAAGATGGGCCACAGCCCTGCCTTTGGATCTATAACTGAAACCATCGTAACAGGGGA CAGGAGTGACTACCTGCTTACAGCACTCGAACCAGAGTCGCCATACCGCGTCTGCATGGTCCCCATGGAAACCAGCAAC ATCTATCTCTCTGACGAGACACCTGAATGCATCGAGACCGAGACAGCTCCCCTCAAGATGTACAACCCGACAACCACAC TCAACCGGGAGCAGGAAAAGGAGCCCTACAAGAACTCCAGCGTGCCTCTGGCCGCCATCATCGGTGGCGCGGTGGCACT GGTGGCCCTGGCACTGCTGGCCCTGGTGTGCTGGTACGTCCACAGGAATGGGGCCCTCTTCTCCCGGCACTGCGCCTAC AGCAAGGGGCGCAGGAGGAAGGACGACTATGCTGAGGCGGGTACCAAGAAGGACAACTCCATTCTAGAAATCAGGGAGA CTTCTTTTCAGATGATACCCATCACTAACGACCAGGTGTCCAAGGAGGAGTTTGTAATACACACCATTTTCCCCCCTAA TGGCATGAATCTGTATAAGAACAGCCACAGTGAAAGCAGTAGTAACAGGAGCTACAGAGACAGTGGTATTCCAGATTCA GATCATTCACACTCATGAT
Citation	Tomás AR, Certal AC, Rodríguez-León J. FLRT3 as a key player on chick limb development. Dev Biol. 2011 Jul 15;355(2):324-33.
Comments	Probe for flrt3 was obtained from chicken EST ChEST840j5.