According to authors , "The chick Flrt3 orthologue was discovered from a search inthe ENSEMBL Gallus gallus genome browser: Identification ENSGALG00000008716. PCR was performed on stage HH16 whole-embryo cDNA to generate two probes that hybridized to different regions of the cFlrt3 gene. Primers used were 1) For: 5- AGATGAATTCCCCACTAACCTC -3 and Rev: 5- ACGTGCGGCCGCATCAGCCCACGTACGTTCAC -3.
2) For: 5- ACGTGAATTCATGACATGAGCACCATCCAG -3
Rev: 5- ACGTGCGGCCGCGGAATACCACTGTCTCTGTAG -3.
Each PCR product was cloned into pBluescript (KS)." As information provided was insufficient to verify exact probe used for photos, the two probes are shown as 5' and 3' reads drawn from NCBI (acc # XM_426107).