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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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PIWIL1
piwi like RNA-mediated gene silencing 1

Gene

Official Symbol	PIWIL1
Official Full Name	piwi like RNA-mediated gene silencing 1
CGNC ID	50348
also known as	CIWI, PIWI, piwi-like protein 1\|piwi-like 1
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_001098852
Polypeptide	NP_001092322
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.16962

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly	3355150			FBgn0250816
Human	9271	ENSG00000125207		MIM:605571
Mouse	57749	ENSMUSG00000029423	All phenotypic alleles (2):Targeted, other(2)	MGI:1928897
Xenopus	100488735			963232
Zebrafish	100331962, 368200	ENSDARG00000041699	ZDB-GENE-030813-2	ZFIN:ZDB-GENE-030813-2

Gene Ontology

Molecular Function	DNA binding, mRNA binding, piRNA binding, single-stranded RNA binding
Biological Process	gene silencing by RNA, meiosis, multicellular organismal development, piRNA metabolic process, more...
Cellular Component	chromatoid body, cytoplasm, mRNA cap binding complex, P granule, polysome

Links to other databases

Entrez Gene	416804
Ensembl Gene	ENSGALG00000002645
KEGG	gga:416804

Entry 1

GEISHA Id	PIWIL1.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	ACTGTCTTTAGCAGATAGGGGCG
Reverse cDNA Template Primer	AATTAACCCTCACTAAAGGCACATTTCCTCTCTTCCTCCGATAC	cDNA Template Length	901
Forward cDNA Template Probe	show GACGGATCAGACAGGCTGGTTCAAGTCACTGATATCTTGGTTATATTGCCTTTTGTAGTAGTCTATATAGCTGATTTCA GAGCCATCTGCTCGTCTAAAAGTACACTGTGGATTGGCATCCCAGTCGATGTCATCAACTCTGTATGTTCTGTTATTGT ACTTCGTAAGAACAATTACACCTATCAGCTCCTTTGCACAGGCATCTCTAAATCTTCTCTCTTCAACCTGTTCATAGAG ACTGTACATAAAATCCAAAACTGTTTCACTACGAAGAATCTTATGGCTCACATCTGCACATAACATAATGCTCTCCTCA TACTGGAGAATAGAACTTGTGAAGCCTGGCCAAACCATCAACCTGTGATTAGGGATGCTGACTGGGTCCTTAGGGTTGT AATAGTTACGTCCAATTTGCTGAAAATTCATCATCTTCAGAAGCCTTCTAAAAATGATGTTGTAAAACTGCAGACATGT AGGTGAAGTAGGTGGTAATTCATTAGTCAATGTGATCGTGATCCTCACATCTTCTCCATTTCGGGTCTTAGAATATACT TCAGTAACCTTATTTGGCAGTCTTTTTGGCAAGAATAATATTGATCCATCAAATGCATGCGTTTTTCCAATTAGGTCTT CATGCTGAAAGAGCAAACCTGATCGAAGGCGGCGTGCTTCCATCTCAGGATTATAGTCTACATGGTATTGATATAAAGC CCATTGGGGTCGAGATGTGAGACGAAAAAAATTTGCAATTAATTTTATCATAGCACCTGAAGAGCCAGTTTTTGATTCT CTAACGTGTTCTATGGCTTGTCGAGTATTTACCCCGAGGTCATGGAAATCCCGACGACGTCCGCCCCTATCTGCAAAAN NACAGT

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 6

Unlabeled

7-12

stage 7

stage 8

stage 10

stage 12

Ectoderm
Endoderm
Lateral Plate Mesoderm
Neural Plate/Tube
Notochord
Paraxial Mesoderm

19-24

stage 19

stage 23

stage 24

Amnion
Ectoderm
Eye
Foregut
Head Mesenchyme
Intermediate Mesoderm
Liver
Lung
Mesonephros
Neural Plate/Tube
Pharyngeal Arches and Clefts
Sclerotome
Somatic Mesoderm
Spinal Ganglia and Nerves
Tail

Dorsal Mesentery
dorsal mesentery

stage 25

Allantois
Foregut
Liver
Lung
Mesonephros
Pharyngeal Arches and Clefts
Spinal Cord
Tail

Entry 2

GEISHA Id	CIWI.Kim.2012	Data Source	Publication
Complete cDNA Template Probe	show `CGTGATGGTGTAGGAGATGGACAGCTCAATACTTTAGTGAACTATGAAGTGCCTCAGTTTCTGGATTGCTTGAAGACTG TTGGTAAAGACTACAATCCAAGACTGACTGTGATCGTTGTGAAGAAACGAGTGAGTACCAGATTCTTTGCGCAGGCTGG TGGAGGACTTAAAAACCCACCCCCTGGTACTGTCGTTGATATAGAGGTGACCAGACCAGAATGGTATGATTTCTTTATT GTGAGTCAGGCAGTGAGAAATGGTTGTGTCGCACCCACTCATTATAACGTAGTGTATGACACTAGCAAACTGAAACCAG ATCATGTACAACGTTTAACCTACAAACTTTGCCACATGTACTATAACTGGTCGGGTGTTATCAGAGTACCTGCTCCTTG CCAGTATGCCCATAAACTGGCTTTCCTTG`
Citation	Kim TH, Yun TW, Rengaraj D, Lee SI, Lim SM, Seo HW, Park TS, Han JY. Conserved functional characteristics of the PIWI family members in chicken germ cell lineage. Theriogenology. 2012 Dec;78(9):1948-59. doi: 10.1016/j.theriogenology.2012.07.019
Copyright	Copyright © 2012 Elsevier Inc. All rights reserved.