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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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GJA1
gap junction protein alpha 1

The human orthologue of this gene is associated
with the following human diseases:

Gene

Official Symbol	GJA1
Official Full Name	gap junction protein alpha 1
CGNC ID	11038
also known as	CX43, connexin-43, gap junction alpha-1 protein\|connexin 43
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204586
Polypeptide	NP_989917
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.3195

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	2697	ENSG00000152661	MIM:600309	MIM:121014
Mouse	14609	ENSMUSG00000050953	All phenotypic alleles (21):Chemically induced(2) Targeted, knock-out(3) Targeted, other(14) Gene trapped(1) Transgenic(1)	MGI:95713
Xenopus	394450, 373664			876601
Zebrafish	30236, 100034754	ENSDARG00000041799	ZDB-GENE-991105-4, ZDB-GENE-050616-10	ZFIN:ZDB-GENE-991105-4, ZFIN:ZDB-GENE-050616-10

Gene Ontology

Molecular Function	transmembrane transporter activity
Biological Process	cell-cell signaling, heart development
Cellular Component	connexon complex, integral component of membrane

Links to other databases

Entrez Gene	395278
Ensembl Gene	ENSGALG00000014873
KEGG	gga:395278

Entry 1

GEISHA Id	GJA1.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	5’-AGAGCTCAAGGTGGTCCAGA-3’
Reverse cDNA Template Primer	5’-AATTAACCCTCACTAAAGGAGAGTTGGAGATGGTGCTGC-3’	cDNA Template Length	655
Complete cDNA Template Probe	show AGAGCTCAAGGTGGTCCAGAATGATGGTGTGAATGTGGATATGCACCTCAAACAAATAGAAAGTAAGAAATTCAAGTAT GGAATTGAAGAGCACGGCAAGGTAAAGATGCGTGGAGGACTGCTTCGTACTTACATCATCAGCATCCTGTTTAAGTCTG TCTTCGAGGTGGCTTTCCTGCTGATACAGTGGTACATTTATGGGTTTAGCCTGAGTGCCATCTACACCTGTGAGCGAGA CCCATGCCCTCACAGGGTGGACTGTTTCCTGTCCCGTCCAACTGAGAAAACCATTTTCATCGTCTTCATGCTGGTAGTG TCTTTGGTGTCTCTTGCCTTGAACATCATTGAGCTTTTCTACGTGTTCTTCAAGGGTGTCAAGGATCGTGTGAAAGGAA AAACCGACCCCTACTCCCACAGTGGCACGATGAGCCCTTCCAAGGACTGTGGTTCCCCCAAATATGCGTATTACAATGG CTGTTCCTCACCGACCGCCCCCTTGTCTCCCATGTCTCCCCCGGGGTACAAGCTTGTTACCGGAGACAGGAACAATTCC TCCTGTCGTAACTACAATAAGCAAGCCAGCGAGCAAAACTGGGCCAACTACAGTGCGGAGCAGAATAGGATGGGGCAGG CTGGCAGCACCATCTCCAACTCT

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Early Mesoderm
Ectoderm
Epiblast
Neural Plate/Tube
Notochord
Primitive Streak

7-12

stage 9

Anterior Neuropore
Ectoderm
Head Mesenchyme
Hindbrain
Intermediate Mesoderm
Lateral Plate Mesoderm
Midbrain
Neural Crest
Neural Plate/Tube
Notochord
Pharynx
Primitive Streak
Somatic Mesoderm
Somites

13-18

stage 13

stage 16

stage 17

Dermomyotome
Ear/Otic Placode
Eye
Forebrain
Head Mesenchyme
Intermediate Mesoderm
Isthmus/MHB
Lateral Plate Mesoderm
Leg Mesenchyme
Liver
Mesonephros
Myotome
Neural Crest
Notochord
Oral Pharynx
Pancreas
Pharyngeal Arches and Clefts
Primitive Streak
Renal
Somatic Mesoderm
Somites
Tail
Widespread Expression
Wing Mesenchyme

19-24

stage 21

stage 23

Allantois
Dermatome
Diencephalon
Ear/Otic Placode
Endoderm
Eye
Forebrain
Foregut
Head Mesenchyme
Hindbrain
Leg Mesenchyme
Liver
Lung
Mesonephros
Midbrain
Myelencephalon
Myotome
Nasal Placode/Nerve
Nephric Ducts
Neural Plate/Tube
Notochord
Pancreas
Pharyngeal Arches and Clefts
Sclerotome
Somatic Mesoderm
Wing Mesenchyme

stage 25

Allantois
Dermatome
Ear/Otic Placode
Foregut
Head Mesenchyme
Leg AER
Leg Mesenchyme
Liver
Myotome
Nasal Placode/Nerve
Pancreas
Pharyngeal Arches and Clefts
Sclerotome
Somatic Mesoderm
Wing AER
Wing Mesenchyme

Entry 2

GEISHA Id	CX43.Berthoud.2004	Data Source	Publication
Complete cDNA Template Probe	show GTGCGTTTCTGGGTTCTGCAGATCATATTTGTGTCTGTACCAACCCTCTTGTACCTGGCGCACGTGTTCTACGTGATGA GGAAAGAAGAGAAGCTGAACAAAAGAGAGGAAGAGCTCAAGGTGGTCCAGAATGATGGTGTGAATGTGGATATGCACCT CAAACAAATAGAAAGTAAGAAATTCAAGTATGGAATTGAAGAGCACGGCAAGGTAAAGATGCGTGGAGGACTGCTTCGT ACTTACATCATCAGCATCCTGTTTAAGTCTGTCTTCGAGGTGGCTTTCCTGCTGATACAGTGGTACATTTATGGGTTTA GCCTGAGTGCCATCTACACCTGTGAGCGAGACCCATGCCCTCACAGGGTGGACTGTTTCCTGTCCCGTCCAACTGAGAA AACCATTTTCATCGTCTTCATGCTGGTAGTGTCTTTGGTGTCTCTTGCCTTGAACATCATTGAGCTTTTCTACGTGTTC TTCAAGGGTGTCAAGGATCGTGTGAAAGGAAAAACCGACCCCTACTCCCACAGTGGCACGATGAGCCCTTCCAAGGACT GTGGTTCCCCCAAATATGCGTATTACAATGGCTGTTCCTCACCGACCGCCCCCTTGTCTCCCATGTCTCCCCCGGGGTA CAAGCTTGTTACCGGAGACAGGAACAATTCCTCCTGTCGTAACTACAATAAGCAAGCCAGCGAGCAAAACTGGGCCAAC TACAGTGCGGAGCAGAATAGGATGGGGCAGGCTGGCAGCACCATCTCCAACTCTCATGCCCAGCCCTTCGACTTCGCCG ATGAGCACCAGAACACGAAAAAACTGGCGTCGGGACACGAGCTGCAACCCCTCACCATTGTGGACCAGAGGCCTCCCAG CAGAGCCAGCAGCCGAGCCAGCAGCAGGCCTCGACCTGATGACCTGGAGATCTA
Citation	Berthoud VM, Singh R, Minogue PJ, Ragsdale CW, Beyer EC. Highly restricted pattern of connexin36 expression in chick somite development. Anat Embryol (Berl). 2004 Nov;209(1):11-8.
Copyright	© Springer-Verlag 2004
Comments	Authors indicated probe sequence shown here.

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Dermatome
Intermediate Mesoderm
Somatic Mesoderm
Spinal Cord
Surface Ectoderm

double label

Entry 3

GEISHA Id	CX43.Reza.2007	Data Source	Publication
Complete cDNA Template Probe	show GCACTTTTCTTTCGCTGGTGGGGAAGGAAGGATGAAAAATACAAAGCAGGAGTAGACTTTTAAAGTTTAAATAGACAGG TCCGAGCGCTTGAGCGTGCTCTTCCGTTTTCCCTTAACCCTCCAGAGAAGTACCCCGAGCCACTCGCACTGCTCATCAG CAGCGCCAATATCCAGTGAAGATGGGTGATTGGAGTGCCCTGGGAAAACTTCTTGACAAGGTTCAAGCTTATTCTACTG CAGGAGGAAAAGTGTGGCTGTCTGTCCTCTTTATTTTCCGAATCCTGCTATTGGGGACAGCAGTCGAGTCTGCTTGGGG AGATGAACAGTCTGCTTTTCGGTGCAATACTCAACAGCCTGGTTGTGAGAACGTCTGCTATGACAAGTCCTTCCCCATC TCCCACGTGCGTTTCTGGGTTCTGCAGATCATATTTGTGTCTGTACCAACCCTCTTGTACCTGGCGCACGTGTTCTACG TGATGAGGAAAGAAGAGAAGCTGAACAAAAGAGAGGAAGAGCTCAAGGTGGTCCAGAATGATGGTGTGAATGTGGATAT GCACCTCAAACAAATAGAAAGTAAGAAATTCAAGTATGGAATTGAAGAGCACGGCAAGGTAAAGATGCGTGGAGGACTG CTTCGTACTTACATCATCAGCATCCTGTTTAAGTCTGTCTTCGAGGTGGCTTTCCTGCTGATACAGTGGTACATTTATG GGTTTAGCCTGAGTGCCATCTACACCTGTGAGCGAGACCCATGCCCTCACAGGGTGGACTGTTTCCTGTCCCGTCCAAC TGAGAAAACCATTTTCATCGTCTTCATGCTGGTAGTGTCTTTGGTGTCTCTTGCCTTGAACATCATTGAGCTTTTCTAC GTGTTCTTCAAGGGTGTCAAGGATCGTGTGAAAGGAAAAACCGACCCCTACTCCCACAGTGGCACGATGAGCCCTTCCA AGGACTGTGGTTCCCCCAAATATGCGTATTACAATGGCTGTTCCTCACCGACCGCCCCCTTGTCTCCCATGTCTCCCCC GGGGTACAAGCTTGTTACCGGAGACAGGAACAATTCCTCCTGTCGTAACTACAATAAGCAAGCCAGCGAGCAAAACTGG GCCAACTACAGTGCGGAGCAGAATAGGATGGGGCAGGCTGGCAGCACCATCTCCAACTCTCATGCCCAGCCCTTCGACT TCGCCGATGAGCACCAGAACACGAAAAAACTGGCGTCGGGACACGAGCTGCAACCCCTCACCATTGTGGACCAGAGGCC TCCCAGCAGAGCCAGCAGCCGAGCCAGCAGCAGGCCTCGACCTGATGACCTGGAGATCTAAGCTTCTCACTGCAGTACT TACTCAACTATGAAACAACGTGCATTGGAAGATGCAGTCAGTGCTGATCTTGTTCCAGTGGAGGTGGTACTTAACAGTC TCAAGCAGGAGATTTTAACAATCGTAAAAACAAACAGGCAAACAAACTTTTAAAATACTTGCTGTTTTTTGGGGAGGAT ATGGGGCTGGTGTGGGGTGGTACAATACACATTGGTATTTAATGTAGCTGGTTTAAA
Citation	Reza HM, Urano A, Shimada N, Yasuda K. Sequential and combinatorial roles of maf family genes define proper lens development. Mol Vis. 2007 Jan 16;13:18-30.
Copyright	This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204586.2).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-16

stage 16

Lens
Retina

Entry 4

GEISHA Id	GJA1.Singh.2016	Data Source	Publication
Complete cDNA Template Probe	show GTAGTGTCTTGGTGTCTCTTGCCTTGAACATCATTGAGCTTTTCTACGTGTTCTTCAAGGGTGTCAAGGATCGTGTGAA AGGAAAAACCGACCCCTACTCCCACAGTGGCACGATGAGCCCTTCCAAGGACTGTGGTTCCCCCAAATATGCGTATTAC AATGGCTGTTCCTCACCGACCGCCCCCTTGTCTCCCATGTCTCCCCCGGGGTACAAGCTTGTTACCGGAGACAGGAACA ATTCCTCCTGTCGTAACTACAATAAGCAAGCCAGCGAGCAAAACTGGGCCAACTACAGTGCGGAGCAGAATAGGATGGG GCAGGCTGGCAGCACCATCTCCAACTCTCATGCCCAGCCCTTCGACTTCGCCGATGAGCACCAGAACACGAAAAAACTG GCGTCGGGACACGAGCTGCAACCCCTCACCATTGTGGACCAGAGGCCTCCCAGCAGAGCCAGCAGCCGAGCCAGCAGCA GGCCTCGACCTGATGACCTGGAGATCTAAGCTTCTCACTGCAGTACTTACTCAACTATGAAACAACGTGCATGGGAAGA TGCAGTCAGTGCTGATCTTGTTCCAGTGGAGGTGGTACTTAACAGTCTCAAGCAGGAG
Citation	Singh PN, Ray A, Azad K, Bandyopadhyay A. A comprehensive mRNA expression analysis of developing chicken articular cartilage. Gene Expr Patterns. 2016 Jan;20(1):22-31.
Copyright	Copyright © 2015 Elsevier B.V. All rights reserved.
Comments	The complete cDNA template sequence was obtained from the information provided in the publication as described in Singh et al. 2016. The probe was synthesized from cDNA clone ChEST644i10. Gja1 is also known as CX43, and connexin-43.