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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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NODAL
nodal growth differentiation factor

Gene

Official Symbol	NODAL
Official Full Name	nodal growth differentiation factor
CGNC ID	49212
also known as	nodal homolog\|nodal-related protein 1, Nodal, NR1, Nodal-related protein 1
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	XM_424385
Polypeptide	XP_424385
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.28

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus	549075, 378543			487168
Zebrafish	799352, 338101	ENSDARG00000057096, ENSDARG00000014309	ZDB-GENE-990415-256, ZDB-GENE-030219-1	ZFIN:ZDB-GENE-990415-256, ZFIN:ZDB-GENE-030219-1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	395205
Ensembl Gene	ENSGALG00000003209
KEGG	gga:395205

Entry 1

GEISHA Id	NODAL.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	CGGCTGGGCAGTGTTCAAC
Reverse cDNA Template Primer	ATTAACCCTCACTAAAGGCACCTGGCTGGGCTTGTAGAG	cDNA Template Length	529
Forward cDNA Template Probe	show CCCCACGCACAGCTCGCAGTACGCGTGATTGGTGGGCTTGAAGCTCTCATCTACAGGTGATGGGCACAGCCCCTCGCAG CGGTAGGCGTTGTACTTCTTGGGGTAGACAATCCAGCTGCCCCAGCCCGTCTGCTCAAAGTCCACCATCATGTCCACCC TCCTGCACAGGGACCTGGCCTCGTCCACCGGGCTGGCAGCGGGAGCATCGCTCACCCTGAGCCTCTGCCTCTCCTTCCT GGTGCGGCGGTGCCGGCGGGTGCCGACATCCCTGGGACCGCCATCACGCATGACGTGCTTGGAGCTCTCCACTGCCCTG AGGAGGCTGTGCTGTCCGGGTGCTTTGTCCTTGGAGAAGACGAGCAGCAGGACGCTGTCTGCCAGCTCCTGGGGCACTG CTGTCTCCTGGGGGTCCGGTGGGACAGTGACCGGGGCGGCTGCAGGATCAGGAGCTGCGTCCTGGTGCAGCCATGCTCT GAGCGTGCTGGTGATGTTGAACACGGCCCAGCCGA

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 25

Aorta
Eye
Foregut
Gut
Mesonephros
Pharyngeal Arches and Clefts
Sclerotome
Sinus Venosus
Spinal Cord
Spinal Ganglia and Nerves

Entry 2

GEISHA Id	Nodal.Mikawa.2004	Data Source	Publication
Complete cDNA Template Probe	show GCTCCCTGCAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGACGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGC TGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCACAACGTCACCGTGGACATCTACCACAGCCCGCGG CCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCTGGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAG TGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACGCAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGT CCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGACAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAA GCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAGCACGTCATGCATGATGGCGGTCCCAGGGATGTCG GCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCAGGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGA CGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTTTGAGCAGACGGGCTGGGGCAGCTGGATCGTCTAC CCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCACCTGTAGACGAGAGCTTCAAGCCCACCAATCACG CGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGCCGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCC CCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCACCACGAGGATATGATCATCGAGGAGTGCGGCTGC AACTGA
Citation	Mikawa, T, Poh, A, Kelly, K, Ishii, Y, Reese, D. Induction and Patterning of the Primitive Streak, an Organizing Center of Gastrulation in the Amniote. Dev Dyn. 2004 Mar;229(3):422-32.
Copyright	Copyright © 2004 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # XM_424385).

Stage

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1-3

stage 3

Primitive Streak

Nodal (bluish purple), Chordin (red)

stage 7

Hensen's Node
Lateral Plate Mesoderm

Nodal (bluish purple), Chordin (red)
abnormal
asymmetrical
bilateral
normal

Entry 3

GEISHA Id	Nodal.Chapman.2002	Data Source	Publication
Complete cDNA Template Probe	show `GAGACAGCAGTGCCCCAGGAGCTGGCAGACAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAAGCACCCGGACAGCACA GCCTCCTCAGGGCAGTGGAGAGCTCCAAGCACGTCATGCGTGATGGCGGTCCCAGGGATGTCGGCACCCGCCGGCACCG CCGCACCAGGAAGGAGAGGCAGAGGCTCAGGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGACGAGGCCAGGTCCCTG TGCAGGAGGGTGGACATGATGGTGGACTTTGAGCAAACGGGCTGGGGCAGCTGGATTGTCTACCCCAAGAAGTACAACG CCTACCGCTGCGAGGGGCTGTGCCCATCACCTGTAGATGAGAGCTTCAAGCCCACCAATCACGCGTACATGCAGAGCCT GCTGCAGCTTTACAAGCCCAGCCAGGTGCCGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCCCCTCTCCATGCTGTAC TATGAGAAGGGT`
Citation	Chapman SC, Schubert FR, Schoenwolf GC, Lumsden A. Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos. Dev Biol. 2002 May 1;245(1):187-99.
Copyright	2002 Elsevier Science (USA). All rights reserved.

Stage

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Location (click to highlight)

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X-XIV

stage XIV

Kohler's Sickle

1-3

stage 3

Primitive Streak

4-6

stage 4

stage 6

Hensen's Node
Primitive Streak

Entry 4

GEISHA Id	NR-1.Levin.1995	Data Source	Publication
Complete cDNA Template Probe	show GCTCCCTGCAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGACGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGC TGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCACAACGTCACCGTGGACATCTACCACAGCCCGCGG CCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCTGGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAG TGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACGCAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGT CCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGACAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAA GCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAGCACGTCATGCATGATGGCGGTCCCAGGGATGTCG GCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCAGGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGA CGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTTTGAGCAGACGGGCTGGGGCAGCTGGATCGTCTAC CCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCACCTGTAGACGAGAGCTTCAAGCCCACCAATCACG CGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGCCGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCC CCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCACCACGAGGATATGATCATCGAGGAGTGCGGCTGC AACTGA
Citation	Levin M, Johnson RL, Stern CD, Kuehn M, Tabin C (1995). A molecular pathway determining left-right asymmetry in chick embryogenesis. Cell. 1995 Sep 8;82(5):803-14.
Copyright	Copyright © 1995 Elsevier Inc. All Rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # XM_424385.2)

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Hensen's Node
Lateral Plate Mesoderm
Mesoderm

7-12

stage 7

stage 8

stage 9

Hensen's Node
Lateral Plate Mesoderm
Trunk Mesenchyme

double labeled

31-44

stage 44

Entry 5

GEISHA Id	Nodal.Hardy.2011	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show GCTCCCTGCAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGACGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGC TGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCACAACGTCACCGTGGACATCTACCACAGCCCGCGG CCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCTGGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAG TGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACGCAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGT CCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGACAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAA GCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAGCACGTCATGCATGATGGCGGTCCCAGGGATGTCG GCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCAGGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGA CGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTTTGAGCAGACGGGCTGGGGCAGCTGGATCGTCTAC CCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCACCTGTAGACGAGAGCTTCAAGCCCACCAATCACG CGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGCCGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCC CCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCACCACGAGGATATGATCATCGAGGAGTGCGGCTGC AACTGA
Comments	Images provided by Katharine M. Hardy and Mary J.C. Hendrix, and funded by NIH award R01CA121205

Stage

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Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XI

stage XIV

Kohler's Sickle

EG&K St. XII

4-6

stage 4

Primitive Streak

7-12

stage 9

stage 10

stage 11

Lateral Plate Mesoderm

13-18

stage 18

19-20

stage 19

stage 20

Entry 6

GEISHA Id	NODAL.Tavares.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCAGCGCCCCCTACCGGCGGCCGGCGGGAAGAGCGGGAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGA CGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGCTGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCA CAACGTCACCGTGGACATCTACCACAGCCCGCGGCCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCT GGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAGTGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACG CAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGTCCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGA CAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAAGCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAG CACGTCATGCATGATGGCGGTCCCAGGGATGTCGGCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCA GGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGACGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTT TGAGCAGACGGGCTGGGGCAGCTGGATCGTCTACCCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCA CCTGTAGACGAGAGCTTCAAGCCCACCAATCACGCGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGC CGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCCCCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCA CCACGAGGATATGATCATCGAGGAGTGCGGCTGCAACTGA
Citation	Tavares AT, Andrade S, Silva AC, Belo JA. Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo. Development. 2007 Jun;134(11):2051-60.
Copyright	copyright 2007 the authors. Published by the Company of Biologists Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # XM_424385.3).

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Lateral Plate Mesoderm

asymmetrical

stage 7

Lateral Plate Mesoderm

asymmetrical

Entry 7

GEISHA Id	NODAL.WangS.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCAGCGCCCCCTACCGGCGGCCGGCGGGAAGAGCGGGAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGA CGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGCTGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCA CAACGTCACCGTGGACATCTACCACAGCCCGCGGCCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCT GGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAGTGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACG CAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGTCCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGA CAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAAGCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAG CACGTCATGCATGATGGCGGTCCCAGGGATGTCGGCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCA GGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGACGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTT TGAGCAGACGGGCTGGGGCAGCTGGATCGTCTACCCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCA CCTGTAGACGAGAGCTTCAAGCCCACCAATCACGCGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGC CGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCCCCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCA CCACGAGGATATGATCATCGAGGAGTGCGGCTGCAACTGA
Citation	Wang S, Yu X, Zhang T, Zhang X, Zhang Z, Chen Y. Chick Pcl2 regulates the left-right asymmetry by repressing Shh expression in Hensen's node. Development. 2004 Sep;131(17):4381-91. Epub 2004 Aug 4.
Copyright	copyright 2004 to the authors. Published by the Company of Biologists Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #XM_424385.3 ).

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Lateral Plate Mesoderm

asymmetric

7-11

stage 11

Lateral Plate Mesoderm

asymmetric

Entry 8

GEISHA Id	NODAL.Schlange.2002	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCAGCGCCCCCTACCGGCGGCCGGCGGGAAGAGCGGGAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGA CGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGCTGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCA CAACGTCACCGTGGACATCTACCACAGCCCGCGGCCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCT GGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAGTGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACG CAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGTCCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGA CAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAAGCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAG CACGTCATGCATGATGGCGGTCCCAGGGATGTCGGCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCA GGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGACGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTT TGAGCAGACGGGCTGGGGCAGCTGGATCGTCTACCCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCA CCTGTAGACGAGAGCTTCAAGCCCACCAATCACGCGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGC CGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCCCCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCA CCACGAGGATATGATCATCGAGGAGTGCGGCTGCAACTGA
Citation	Schlange T, Arnold HH, Brand T. BMP2 is a positive regulator of Nodal signaling during left-right axis formation in the chicken embryo. Development. 2002 Jul;129(14):3421-9.
Copyright	Copyright is held by the authors. Published by the Company of Biologists Ltd.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #XM_424385.3 )

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 7

Lateral Plate Mesoderm

double labeled

Entry 9

GEISHA Id	NODAL.Katsu.2012	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCAGCGCCCCCTACCGGCGGCCGGCGGGAAGAGCGGGAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGA CGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGCTGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCA CAACGTCACCGTGGACATCTACCACAGCCCGCGGCCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCT GGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAGTGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACG CAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGTCCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGA CAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAAGCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAG CACGTCATGCATGATGGCGGTCCCAGGGATGTCGGCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCA GGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGACGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTT TGAGCAGACGGGCTGGGGCAGCTGGATCGTCTACCCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCA CCTGTAGACGAGAGCTTCAAGCCCACCAATCACGCGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGC CGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCCCCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCA CCACGAGGATATGATCATCGAGGAGTGCGGCTGCAACTGA
Citation	Katsu K, Tokumori D, Tatsumi N, Suzuki A, Yokouchi Y. BMP inhibition by DAN in Hensen's node is a critical step for the establishment of left-right asymmetry in the chick embryo. Dev Biol. 2012 Mar 1;363(1):15-26.
Copyright	Copyright © 2012. Published by Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (Acc. # XM_424385.3).

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 6

Paraxial Mesoderm

control embryo

7-8

stage 7

stage 8

Area Pellucida
Hensen's Node
Lateral Plate Mesoderm

control embryo

Entry 10

GEISHA Id	NODAL.Tsikolia.2012	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show ATGGCAGCAGCGCCCCCTACCGGCGGCCGGCGGGAAGAGCGGGAGAATGGCTCCCGCTGGGCACTCTCCTTTGACATGA CGTCCCTGTCTGGAAGCCAGGAGGTGAGCCTGGCTGAGCTTCGCATCCGCCCGCACGCTCTGTCCTTTTCCCTGTCCCA CAACGTCACCGTGGACATCTACCACAGCCCGCGGCCGCCGTGCCGGGGCACCAAAGACCTCTTCCTGGGCACCGTGGCT GGTGGCCCCTCATCCCACCAGGCCGGCTGGGCAGTGTTCAACATCACCAGCACGCTCAGAGCATGGCTGCACCAGGACG CAGCTCCTGATCCTGCAGCCGCCCCGGTCACTGTCCCACCGGACCCCCAGGAGACAGCAGTGCCCCAGGAGCTGGCAGA CAGCGTCCTGCTGCTCGTCTTCTCCAAGGACAAAGCACCCGGACAGCACAGCCTCCTCAGGGCAGTGGAGAGCTCCAAG CACGTCATGCATGATGGCGGTCCCAGGGATGTCGGCACCCGCCGGCACCGCCGCACCAGGAAGGAGAGGCAGAGGCTCA GGGTGAGCGATGCTCCCGCTGCCAGCCCGGTGGACGAGGCCAGGTCCCTGTGCAGGAGGGTGGACATGATGGTGGACTT TGAGCAGACGGGCTGGGGCAGCTGGATCGTCTACCCCAAGAAGTACAACGCCTACCGCTGCGAGGGGCTGTGCCCATCA CCTGTAGACGAGAGCTTCAAGCCCACCAATCACGCGTACATGCAGAGCCTGCTGCAGCTCTACAAGCCCAGCCAGGTGC CGTGCCCTGCCTGCTCGCCCATCAGGATGAGTCCCCTCTCCATGCTGTACTATGAGAAGGGTGAGATCGTCGTCCGCCA CCACGAGGATATGATCATCGAGGAGTGCGGCTGCAACTGA
Comments	According to the authors, "Digoxigenin-labeled mRNA probes for nodal, shh, fgf8 were produced using the plasmid DNAs previously published ( Boettger et al., 1999, Essner et al., 2002 and Levin et al., 1995)" As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence was obtained from NCBI (acc# XM_424385.3).