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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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OTX2
orthodenticle homeobox 2

The human orthologue of this gene is associated
with the following human diseases:

Gene

Official Symbol	OTX2
Official Full Name	orthodenticle homeobox 2
CGNC ID	14164
also known as	homeobox protein OTX2\|orthodenticle homolog 2, homeobox protein OTX2 , Otx2 , orthodenticle homeobox 2
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204520
Polypeptide	NP_989851
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.10

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	5015		MIM:613986	MIM:600037
Mouse	18424	ENSMUSG00000021848	All phenotypic alleles (32):Targeted, knock-out(11) Targeted, other(21)	MGI:97451
Xenopus	548931, 432013, 399342			485219
Zebrafish	30501	ENSDARG00000011235	ZDB-GENE-980526-406	ZFIN:ZDB-GENE-980526-406

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	395191
Ensembl Gene
KEGG	gga:395191

Entry 1

GEISHA Id	Otx2	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CATGGTTTTTTTTTCACACACCAACATTTATTCCAAGACACGTTACAGCTGGGAAGAGGCGCACACGTTTCACTCCTGG CTCTCAGGCAAAACATCACAGATCAGCCCCTTCCAGGCCCCATAGCAGCCCCTTCCAGGCCCCATAGCAGCCCCTTCCA GGCCCCATAGCAGCCCCTTCCAGGCCCCCCACAAAGGCTCGGGCACTTCTAACAAGCTTCCAAGCCACCCCCAAACAAG CATCCTGCCCACAAGGCACCGTTCCCCACACACTGGGACCCACCCTGGGTCAGAAACGCAGCTCCTGCCCTGCCCCACA GCACCCACAAGCAGAGCGCTGCCATCCAGCTGTGCTCAGAGCTGTTAGGGTCGCTGAATTTGGAGAGGACCCGACCGGC GGCTATGCCCCCCGCGCCCCCCAGCCGTGCCAGCGGCCCCGCAGGCACCTGGCGGCCCCCCCCTCCGCCCGACGGCACC CCCAGCACCGCCCGGGGCAGGCAGGGCGCTCGGGCGGACCCCCCTCGCCCGGATTTCGGCCAACTCTCCTCCCCGCTCC AACTTTAGCATGATGTCTTATCTTACGCAACCACCTTACGCAGTCATTGGCTTGAGTTTCACCACTTCGGGCATGGATT TGTTGCATCCGTCAGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACCACCTTCACCCGGGCGCAGCT GGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGGAGGTGGCCTTGAAAATCAAC CTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCAGCAGCAGCAGCAGCAGAACG GGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGCTCGGAGAGCGGGACCAGCGG GCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCGTGTCCATCTGGAGCCCGGCG TCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAAGTCCTACCCCATGACCTACACCCAGG CATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGTGGATCTTATTTGACCCCTAT GCACCACCAGCTCCCCGGACCGGGGGCCACCCTGATTCCCATGGGTGCCAATGCGGTCACCAGCCACCTCAACCATTCT CCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCATTTTGGGCTTTAACTCAACCACCGATTGCTTGGATTATAAAA ACCAAACCGCTTCTTGGAATTTAAACTTCAATGCTGACTGCTTGGATTATAAAAACCAAACTTCCTCATGGAAGTTCCA GGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATTGTGACGAGAACAAAACACGCTGGGCCCGAACGT TCCAGCTTTAGTCAGGTTTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGACAAACAAATGAACATAAATC CGACGACGACAACAACGGGGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTCATCTCCTGCTCAGACGCTC TGGAAAAGGAACGACAAAGAGGAAAACGGAGGAGGAAGGCCTTTAAAACGGACAGAATCATTTAGTGAGGCAGAAAACC GGACAGACCAATCCGTGTTCTTTAATAGTTTTAGGCAATTTGTTGGGGTTTCTTTTGTTTGGAAGAGGTGGGAGATCGT TCCCACCTATGGGCCAGTTTTTAAACAAAAAAAAAAAAGTCTAGGTTTTTATTTCTTTTTGTGTGTGCGTGGAAAGATC CTTCACCCAGAGGTTTCCAACGTCTTAGCCAACCCTTGGTTAAAATATTCGGAATGGACAGCGTCTCTCTTTCTCTTTT TCTCCCCTTTTTTCTCCCTCCTTCCCTCCCTCTTTCTTTCTCTCTCGCGCTCATCCAACTGTTTGACGCCCAACTCAAG TGGGCACCGAGAGAACTTGGTTCAGGGCTGTGTGGGTTGTGTGACTGATTGTCCTAGCTGCACTACTTTATTTAAAGAA AAAAAAAATAATGTTCATAAGGAGTCAATATGTAGTTTTTAAGAGACAATCAGTGTGTGTCTTATATAAATGGTACATC TGTGGTTTTTAATCTGTGCTAGACTTCAAAACTGTGATCTCCTGTATTGTATGCAACTACGAACTCCGCACCAGCGGGA GTTCCGCTGTGATTTAAATAGGTTATAATTATAAGTGAAATTCAGAGCAACTGAGTTACCTATTATCATCTTTCTAAAA A
Comments	Images provided by Susan Chapman

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Forebrain
Midbrain

13-17

stage 17

Cranial Ganglia
Forebrain
Midbrain
Pharyngeal Arches and Clefts

Entry 2

GEISHA Id	Otx2.Chapman.2002	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Chapman SC, Schubert FR, Schoenwolf GC, Lumsden A. Analysis of spatial and temporal gene expression patterns in blastula and gastrula stage chick embryos. Dev Biol. 2002 May 1;245(1):187-99.
Copyright	2002 Elsevier Science (USA). All rights reserved.

Stage

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Location (click to highlight)

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X-XIV

stage XIII

stage XIV

Epiblast
Hypoblast

1-3

stage 3

Endoderm
Head Process
Hensen's Node
Neural Plate/Tube
Primitive Streak

4-5

stage 4

stage 5

Early Endoderm
Ectoderm
Epiblast
Forebrain
Midbrain
Neural Plate/Tube

Entry 3

GEISHA Id	OTX2.Ohuchi.2007	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Ohuchi H, Hayashibara Y, Matsuda H, Onoi M, Mitsumori M, Tanaka M, Aoki J, Arai H, Noji S. Diversified expression patterns of autotaxin, a gene for phospholipid-generating enzyme during mouse and chicken development. Dev Dyn. 2007 Apr;236(4):1134-43.
Copyright	Copyright © 2007 Wiley-Liss, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204520).

Stage

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Location (click to highlight)

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13-15

stage 15

Diencephalon
Midbrain

Entry 4

GEISHA Id	Otx2.Abe.2006	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Abe Y, Chen W, Huang W, Nishino M, Li YP. CNBP regulates forebrain formation at organogenesis stage in chick embryos. Dev Biol. 2006 Jul 1;295(1):116-27. Epub 2006 Apr 19.
Copyright	Reprinted from Developmental Biology, 295, Yoko Abea, Wei Chena, Wei Huangc, Mizuho Nishinod and Yi-Ping Li, CNBP regulates forebrain formation at organogenesis stage in chick embryos, 116-127, Copyright 2006, with permission from Elsevier. Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204520)

Stage

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Location (click to highlight)

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19-21

stage 19

stage 21

Forebrain
Midbrain

Entry 5

GEISHA Id	Otx2.vonFrowein.2006	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Comments	Authors said "Plasmid templates were used to generate digoxygenin antisense riboprobes: Otx2 (Wassef et al., 1987). However, no Otx2 clone is listed in this paper. As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204520).

Stage

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Location (click to highlight)

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19-24

stage 22

Forebrain

25-28

stage 28

Forebrain

Entry 6

GEISHA Id	Otx2.Sanchez-Calderon.2007	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Sánchez-Calderón H, Francisco-Morcillo J, Martín-Partido G, Hidalgo-Sánchez M (2007). Fgf19 expression patterns in the developing chick inner ear.Gene Expr Patterns. Jan;7(1-2):30-8. Epub 2006 May 19.
Copyright	Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204520).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 15

Ear/Otic Placcode

Figure 1. includes HH stage 14 and 15. Also, includes comparison of Fgf19 expression to Fgf8, Fgf10 and Otx2 expression in chick embryos.

19-24

stage 20

stage 24

Ear/Otic Placcode

Figure 2 includes comparison of Fgf19 expression to Fgf8, Otx2 and Bmp4 expression in stage 20 chick embryos.
Figure 3. includes comparison of Fgf19 expression to Fgf8, Otx2 and Bmp4 expression in stage 24 chick embryos.

Entry 7

GEISHA Id	Otx2..Badde.2005	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Badde A, Bumsted-O'Brien KM, Schulte D. Chick receptor protein tyrosine phosphatase lambda/psi (cRPTPlambda/cRPTPpsi) is dynamically expressed at the midbrain-hindbrain boundary and in the embryonic neural retina. Gene Expr Patterns. 2005 Aug;5(6):786-91.
Copyright	Copyright © 2005 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204520).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-16

stage 15

stage 16

Forebrain
Midbrain

Entry 8

GEISHA Id	Otx2.Chapman.2008	Data Source	Direct Submission
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Comments	Insufficient information provided to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204520). Images Provided by Susan Chapman.

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

stage 3

Area Pellucida
Epiblast
Hensen's Node
Neural Plate/Tube
Primitive Streak

4-6

stage 4

stage 5

stage 6

Epiblast
Forebrain
Hensen's Node
Midbrain
Neural Plate/Tube
Primitive Streak

7-12

stage 7

stage 8

stage 9

stage 10

stage 12

Forebrain
Midbrain
Neural Plate/Tube

13-17

stage 17

Cranial Ganglion
Forebrain
Midbrain
Neural Plate/Tube

Entry 9

GEISHA Id	OTX2.Agarwala.2005	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Agarwala S, Aglyamova GV, Marma AK, Fallon JF, Ragsdale CW. Differential susceptibility of midbrain and spinal cord patterning to floor plate defects in the talpid2 mutant. Dev Biol. 2005 Dec 1;288(1):206-20. Epub 2005 Oct 21.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	According to the authors, "Digoxigenin (DIG)- and fluorescein-labeled riboprobes were synthesized from chick cDNA plasmids..." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_204520).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-26

stage 26

Isthmus/MHB

Entry 10

GEISHA Id	Otx-2.Albazerchi.2007	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Albazerchi A, Stern CD. A role for the hypoblast (AVE) in the initiation of neural induction, independent of its ability to position the primitive streak. Dev Biol. 2007 Jan 15;301(2):489-503.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #NM_204520.2). Although experiments are shown, normal expression can be seen in the embryo to the left.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Forebrain
Hensen's Node
Neural Plate/Tube

4-5

stage 4

stage 5

Forebrain
Neural Plate/Tube
Primitive Streak

Entry 11

GEISHA Id	OTX1.Plouhinec.2005	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Plouhinec JL, Leconte L, Sauka-Spengler T, Bovolenta P, Mazan S, Saule S (2005). Comparative analysis of gnathostome Otx gene expression patterns in the developing eye: implications for the functional evolution of the multigene family. Dev Biol. 2005 Feb 15;278(2):560-75.
Copyright	Copyright © 2004 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify sequence used to synthesize probe. NCBI lists Otx1 as a conserved domain in Otx2. Sequence below was obtained from NCBI (NM_204520).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 8

stage 10

stage 12

Ear/Otic Placcode
Ectoderm
Eye
Forebrain
Midbrain
Surface Ectoderm

Neural Ectoderm

13-18

stage 15

stage 16

Eye
Forebrain
Midbrain
Retina

Marginal zone of the Neural Retina
Prospective Pigmented Retina

19-24

stage 20

stage 22

Retina

Marginal zone of the Neural Retina
Prospective Pigmented Retina

31-44

stage 35

stage 37

stage 40

stage 44

Eye
Retina

Ciliary Process
Prospective Iris

Entry 12

GEISHA Id	OTX2.Plouhinec.2005	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Plouhinec JL, Leconte L, Sauka-Spengler T, Bovolenta P, Mazan S, Saule S (2005). Comparative analysis of gnathostome Otx gene expression patterns in the developing eye: implications for the functional evolution of the multigene family. Dev Biol. 2005 Feb 15;278(2):560-75.
Copyright	Copyright © 2004 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204520).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 16

Eye
Forebrain
Midbrain
Pharyngeal Arches and Clefts
Retina

Prospective Pigmented Retina

19-24

stage 22

Eye
Retina

Prospective Pigmented Retina

25-30

stage 26

stage 30

Retina

31-44

stage 37

stage 40

stage 44

Retina

Actual Stage: P2

Entry 13

GEISHA Id	OTX2.Zhang.2001	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Zhang XM, Yang XJ (2001). Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis. Dev Biol. 2001 May 15;233(2):271-90.
Copyright	Copyright © 2001 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204520).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 22

stage 24

Eye

Pigmented Epithelium

Entry 14

GEISHA Id	OTX2.Foley.2000	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Foley AC, Skromne I, Stern CD. Reconciling different models of forebrain induction and patterning: a dual role for the hypoblast. Development. 2000 Sep;127(17):3839-54.
Copyright	Printed in Great Britain © The Company of Biologists Limited 2000
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204520).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XIII

Hypoblast

Stage XII-XIII EG&K

1-3

stage 2

Hypoblast
Primitive Streak

stage 4

Hypoblast
Primitive Streak

Entry 15

GEISHA Id	OTX2.Spieler.2004	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Spieler D, Bäumer N, Stebler J, Köprunner M, Reichman-Fried M, Teichmann U, Raz E, Kessel M, Wittler L. Involvement of Pax6 and Otx2 in the forebrain-specific regulation of the vertebrate homeobox gene ANF/Hesx1. Dev Biol. 2004 May 15;269(2):567-79.
Copyright	Copyright © 2004 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204520).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Neural Plate/Tube

7-8

stage 8

Neural Plate/Tube

Anterior Neural Folds

Entry 16

GEISHA Id	OTX2.Bok.2005	Data Source	Publication
Complete cDNA Template Probe	show CAAATGGACAAACAAATGAACATAAATCCGACGACGACAACAACGGGGACAAAAGAGGGACAGTGTTGGTGTGACCACG TTCAGACTCATCTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAGAGGAAAACGGAGGAGGAAGGCCTTTAAAACGG ACAGAATCATTTAGTGAGGCAGAAAACCGGACAGACCAATCCGTGTTCTTTAATAGTTTTAGGCAATTTGTTGGGGTTT CTTTTGTTTGGAAGAGGTGGGAGATCGTTCCCACCTATGGGCCAGTTTTTAAACAAAAAAAAAAAAGTCTAGGTTTTTA TTTCTTTTTGTGTGTGCGTGGAAAGATCCTTCACCCAGAGGTTTCCAACGTCTTAGCCAACCCTTGGTTAAAATATTCG GAATGGACAGCGTCTCTCTTTCTCTTTTTCTCCCCTTTTTTCTCCCTCCTTCCCTCCCTCTTTCTTTCTCTCTCGCGCT CATCCAACTGTTTGACGCCCAACTCAAGTGGGCACCGAGAGAACTTGGTTCAGGGCTGTGTGGGTTGTGTGACTGATTG TCCTAGCTGC
Citation	Bok J, Bronner-Fraser M, Wu DK. Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear. Development. 2005 May;132(9):2115-24. Epub 2005 Mar 23.
Copyright	Copyright for articles in Development are retained by the authors. Permission granted by MBF.
Comments	According to the authors, "A 562 bp of 3′-untranslated region (nucleotides 1183-1745) of chicken Otx2 (GenBank: AJ489221) was used to generate an antisense probe for Otx2." Sequence below was obtained from NCBI using this information.

no images

Entry 17

GEISHA Id	OTX2.Sanchez-Calderon.2004	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Sánchez-Calderón H, Martín-Partido G, Hidalgo-Sánchez M. Otx2, Gbx2, and Fgf8 expression patterns in the chick developing inner ear and their possible roles in otic specification and early innervation. Gene Expr Patterns. 2004 Oct;4(6):659-69.
Copyright	Reprinted with permission from Elsevier. Copyright © 2004 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204520.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-30

stage 27

Ear/Otic Placode

31-34

stage 34

Ear/Otic Placode

Entry 18

GEISHA Id	OTX2.Sanders.2002	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Sanders TA, Lumsden A, Ragsdale CW. Arcuate plan of chick midbrain development. J Neurosci. 2002 Dec 15;22(24):10742-50.
Copyright	Copyright © 2002-2011 by the Society for Neuroscience
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc# NM_204520.2)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-26

stage 26

Forebrain
Hindbrain
Midbrain
Rhombomeres

Hindbrain Expression is localized to the caudal end of Rhombomere 1.

Entry 19

GEISHA Id	OTX2.Halilagic.2007	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Halilagic A, Ribes V, Ghyselinck NB, Zile MH, Dollé P, Studer M. Retinoids control anterior and dorsal properties in the developing forebrain. Dev Bio 2007 Mar 1;303(1):244-58
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc #NM_204520.2). Images are quail, but sequence and map shown are chick.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 14

Eye

quail embryo

stage 19

Retina

quail embryo

Entry 20

GEISHA Id	OTX2.Tossell.2011	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Tossell K, Kiecker C, Wizenmann A, Lang E, Irving C. Notch signalling stabilises boundary formation at the midbrain-hindbrain organiser. Development. 2011 Sep;138(17):3745-57. Epub 2011 Jul 27.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204520.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-10

stage 9

stage 10

Isthmus/MHB
Mesencephalon
Midbrain
Rhombomeres

double in situ: Dl1 (blue) and Otx2 (red)
double in situ: Otx2 (red) and Gbx2 (blue)
double in situ: Lfng (blue), Otx2 (red)
double in situ: Otx2 (red), Gbx2(blue)
double in situ: Ser1 (blue), Otx2 (red)

Entry 21

GEISHA Id	OTX2.Tossell.2011B	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Tossell K, Andreae LC, Cudmore C, Lang E, Muthukrishnan U, Lumsden A, Gilthorpe JD, Irving C. Lrrn1 is required for formation of the midbrain-hindbrain boundary and organiser through regulation of affinity differences between midbrain and hindbrain cells in chick. Dev Biol. 2011 Apr 15;352(2):341-52.
Copyright	Copyright © 2011 Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204520.2).

no images

Entry 22

GEISHA Id	OTX2.Lorente-Cánovas.2012	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Lorente-Cánovas B, Marín F, Corral-San-Miguel R, Hidalgo-Sánchez M, Ferrán JL, Puelles L, Aroca P. Multiple origins, migratory paths and molecular profiles of cells populating the avian interpeduncular nucleus. Dev Biol. 2012 Jan 1;361(1):12-26.
Copyright	Copyright © 2011 Elsevier Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204520.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

25-30

stage 27

stage 29

Rhombomeres

PAX7 antibody (brown)
in situ hybridization with OTX2 (blue)

31-39

stage 36

stage 39

Rhombomeres

PAX7 antibody (brown)
in situ hybridization with OTX2 (blue)

Entry 23

GEISHA Id	OTX2.Acloque.2011	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Hervé Acloque, Oscar H. Ocaña, Ander Matheu, Karine Rizzoti, Clare Wise, Robin Lovell-Badge, M. Angela Nieto Reciprocal Repression between Sox3 and Snail Transcription Factors Defines Embryonic Territories at Gastrulation. Dev Cell. 2011 September 13; 21(3): 546558.
Copyright	Copyright © 2011 Published by Elsevier Inc.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204520.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Neural Plate/Tube

Entry 24

GEISHA Id	OTX2.Adams.2000	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Adams KA, Maida JM, Golden JA, Riddle RD. The transcription factor Lmx1b maintains Wnt1 expression within the isthmic organizer. Development. 2000 May;127(9):1857-67.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors.
Comments	As insufficient information is provided in the publication to verify the exact sequence used to synthesize the probe, the sequence below was obtained from NCBI (acc # NM_204520.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

Diencephalon
Mesencephalon

13-15

stage 15

Mesencephalon

Entry 25

GEISHA Id	OTX2.Hirashima.2008	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Hirashima M, Kobayashi T, Uchikawa M, Kondoh H, Araki M. Anteroventrally localized activity in the optic vesicle plays a crucial role in the optic development. Dev Biol. 2008 May 15;317(2):620-31. doi: 10.1016/j.ydbio.2008.03.010. Epub 2008 Mar 20.
Copyright	Copyright © 2008 Elsevier Inc. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI EF410002.1

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-11

stage 11

Eye

Entry 26

GEISHA Id	OTX2.Miyazaki.2006	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Miyazaki H, Kobayashi T, Nakamura H, Funahashi J. Role of Gbx2 and Otx2 in the formation of cochlear ganglion and endolymphatic duct. Dev Growth Differ. 2006 Sep;48(7):429-38.
Copyright	Copyright © 19992013 John Wiley & Sons, Inc. All Rights Reserved.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 11

13-18

stage 14

19-22

stage 20

stage 22

Entry 27

GEISHA Id	OTX2.Marcucio.2005	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Marcucio RS, Cordero DR, Hu D, Helms JA. Molecular interactions coordinating the development of the forebrain and face. Dev Biol. 2005 Aug 1;284(1):48-61.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence was obtained from NCBI (NM_204520.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-22

stage 22

Hypothalamus
Telencephalon

all

Entry 28

GEISHA Id	Otx2.Jungbluth.2001	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Stefan Jungbluth, Chris Phelps, Andrew Lumsden. CEPU-1 expression in the early embryonic chick brain. Mech Dev. 2001 Mar;101(1-2):195-7.
Copyright	Copyright © 2001 Elsevier Science Ireland Ltd.
Comments	Authors indicated that Otx2 probe was a gift of A. Simeone. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence above was obtained from NCBI (accession NM_204520.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Forebrain
Isthmus/MHB
Midbrain

double in situ: Otx2 in red, CEPU-1 in blue

13-18

stage 14

Forebrain
Isthmus/MHB
Midbrain

double in situ: Otx2 in red, CEPU-1 in blue

stage 19

Cranial Ganglia
Forebrain
Isthmus/MHB
Midbrain
Pharyngeal Arches and Clefts

double in situ: Otx2 in red, CEPU-1 in blue

Entry 29

GEISHA Id	Otx2.Hidalgo-Sánchez.1999	Data Source	Publication
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Citation	Hidalgo-Sánchez M, Millet S, Simeone A, Alvarado-Mallart RM. Comparative analysis of Otx2, Gbx2, Pax2, Fgf8 and Wnt1 gene expressions during the formation of the chick midbrain/hindbrain domain. Mech Dev 1999 Mar;81(1-2):175-8.
Copyright	Copyright © 1999 Elsevier Science Ireland Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence above was obtained from NCBI (accession NM_204520.2).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

Midbrain

Double ISH, Otx2 in purple.
Double ISH, Otx2 in red.

19-20

stage 20

Midbrain

Double ISH, Otx2 in purple.
Double ISH, Otx2 in red.
Double ISH, Otx2 in red.

Entry 30

GEISHA Id	OTX2.Trevers.2023	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CCAACTCTCCTCCCCGCTCCAACTTTAGCATGATGTCTTATCTTAAGCAACCACCTTACGCAGTCAATGGCCTGAGTCT CACCACCTCGGGCATGGATTTGTTGCATCCGTCCGTCGGTTATCCCGCCACCCCCCGAAAGCAGCGGCGGGAGCGCACC ACCTTCACCCGGGCGCAGCTGGACGTGCTGGAGGCCCTGTTCGCCAAGACCCGCTACCCCGACATCTTCATGCGGGAGG AGGTGGCCTTGAAAATCAACCTGCCCGAGTCCAGAGTGCAGGTGTGGTTCAAAAACCGCCGGGCCAAGTGCCGGCAGCA GCAGCAGCAGCAGCAGAACGGGGGCCAGAACAAGGTGAGGCCGGCCAAAAAGAAGAACTCGCCGGCCCGGGAAGTGAGC TCGGAGAGCGGGACCAGCGGGCAGTTCACACCCCCCTCCAGCACCTCGGTCCCCACCATTTCCAGCAGCAGTGCCCCCG TGTCCATCTGGAGCCCGGCGTCCATCTCCCCGCTCTCCGATCCCCTGTCCACTTCTTCCTCCTGCATGCAGAGGTCCTA CCCCATGACCTACACCCAGGCATCAGGTTACAGCCAAGGATATGCCGGCTCGACCTCCTATTTCGGAGGGATGGACTGT GGATCTTATTTGACCCCTATGCACCACCAGCTCCCCGGACCGGGGGCCACCCTGAGTCCCATGGGTGCCAATGCGGTCA CCAGCCACCTCAACCAGTCTCCAGCCTCCCTCTCCACCCAGGGCTATGGAGCCTCCAGTTTGGGCTTTAACTCGACCAC CGATTGCTTGGATTATAAAGACCAAACCGCCTCCTGGAAGTTAAACTTCAATGCTGACTGCTTGGATTATAAAGACCAG ACATCCTCATGGAAGTTCCAGGTTTTGTGAAGGCCCGTGGGAACCTTTGTGATAATAAGAGAAATCGTGACGAGAACAA AACACGCTGGGCCGAACGCTCCAGCTTTAGTCAGGTCTTGGTTAAATTAAAGAGAAAATAACTAAATGGACAAATGGAC AAACAAATGAACATAAATCCGACGACGACAACAACGGCGACAAAAGAGGGACAGTGTTGGTGTGACCACGTTCAGACTC ATTTCCTGCTCAGACGCTCTGGAAAAGGAACGACAAAAGAGGAAAACGGAGGAGGAAGGCCTTAAACGGACAGATCATC TAGTGAGCAGAAAACAGACAGACCAATCCGTGTTCTTTATAGTTTTAGGCAATTGTTGGGTTTCTTTGTTTGGAAAAGG TGGGAGATCGTTCCACCTATGGGCCAGTTTTTAAAAAAAAA
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204520 ).